Multiplexed Crispr Libraries For Cancer Functional Genomics,
2020
The University of Texas MD Anderson Cancer Center UTHealth Graduate School of Biomedical Sciences
Multiplexed Crispr Libraries For Cancer Functional Genomics, Jintan Liu
Dissertations and Theses (Open Access)
High-throughput forward genetic screenings are invaluable tools to systematically explore genetic interactions and to link gene disruption with disease contexts. The adaptation of CRISPR/Cas9 has improved the sensitivity and specificity of functional screenings. Despite this advance, there remains a long-standing need to improve functional screenings with smaller and more versatile pooled libraries. Capitalizing on the inherent multiplexing capability of a class 2 CRISPR enzyme AsCpf1, we developed a multiplexed, high throughput screening strategy that has avoided the usual trade-off between library size and library penetration, allowing library minimization without sacrificing gene targeting efficiency. We optimized the AsCpf1 protein for functional …
Editing Fusarium Graminearum Using Crispr/Cas9,
2020
University of Northern Iowa
Editing Fusarium Graminearum Using Crispr/Cas9, Grace Marie Sack
Honors Program Theses
Fusarium graminearum is a pathogenic fungus that causes scab or Fusarium head blight disease in barley and wheat. Alternative approaches to protect barley from this devastating disease are badly needed. In recent years, an RNA-guided genome-editing system known as clustered regularly interspaced short palindromic repeat and CRISPR-associated protein 9 (CRISPR/Cas9), has become a popular method for genome modification (Jinek et al., 2012). We applied CRISPR/Cas9 technology in an effort to make F. graminearum less infectious. We used CRISPR/Cas9 plasmids constructed by a former UNI student (Akers, 2019) to mutate three genes in F. graminearum. One of the genes ( …
Understanding Forest Fire Management In Indonesia From A Global Perspective,
2020
Universiti Brunei Darussalam
Understanding Forest Fire Management In Indonesia From A Global Perspective, Riyanto Indra Agus, Cahyadi Ahmad, Kurniadhini Faricha, Bachtiar Hafidz, Apriyana Dwiki, Caraka Brian Kannardi Aji
ASEAN Journal on Science and Technology for Development
Forest fires are one of the global issues that attract worldwide attention. Russia, Brazil, Canada, the United States, and Indonesia are among the countries with the largest forest cover and long records of massive forest fires. Forest fire management is, therefore, critical to decreasing the severity level of these fires. Current conditions indicate that, compared with the four other countries, Indonesia has significantly reduced forest fires within the past five years. Consequently, adopting a global perspective to study the characteristics of forest fire disaster management has become necessary. For each management parameter, this research employed a literature review and descriptive …
The Indonesian Throughflow And Its Impact On Biogeochemistry In The Indonesian Seas,
2020
Universiti Brunei Darussalam
The Indonesian Throughflow And Its Impact On Biogeochemistry In The Indonesian Seas, Taufiqurrahman Edwards, Wahyudi A’An J., Masumoto Yukio
ASEAN Journal on Science and Technology for Development
It has been widely known that the Indonesian Throughflow (ITF) is an important inter-ocean connection with unique and complex oceanographic and geographic conditions, as well as a strong relation to both regional and global ocean currents and climate systems. Many studies on characteristics, mechanisms, and impacts of the ITF have been conducted, mainly focusing on the ITF pathways, transport, water mass mixing processes, and their variability in connection with monsoons and climate systems. In this paper, we summarize some of the critical aspects related to ocean conditions within the Indonesian Seas and the Indonesian Throughflow, with the main focus on …
Anthropogenic Signatures In The Context Of Landscape Evolution: Evidence From Citanduy Watershed, Java, Indonesia,
2020
Universiti Brunei Darussalam
Anthropogenic Signatures In The Context Of Landscape Evolution: Evidence From Citanduy Watershed, Java, Indonesia, Malawani Mukhamad Ngainul, Mardiatno Djati, Haryono Eko
ASEAN Journal on Science and Technology for Development
The impact of humans on landscapes may differ across regions because anthropogenic intervention on the respective landscapes occurs in different human-induced contexts. This study was designed to assess the human-induced landscape evolution of the Citanduy Watershed, Java, Indonesia, and determine its anthropogenic signatures. Several approaches were used, namely anthropogenic landscape identification, anthropogenic soil development, sediment analysis, and monitoring of changes to Segara Anakan lagoon at the mouth of the Citanduy Rier. Five types of anthropogenic landscapes were observed in the research area, from a slightly modified landscape to a fully anthropogenic landscape. The anthropogenic signature was also found in the …
Comparison Of 3d Coral Photogrammetry And Coral Video Transect For Coral Lifeform Analysis Using Low-Cost Underwater Action Camera,
2020
Universiti Brunei Darussalam
Comparison Of 3d Coral Photogrammetry And Coral Video Transect For Coral Lifeform Analysis Using Low-Cost Underwater Action Camera, Ahmad Zuhairi Bin, Mohd Jinah Muhammad Idzham Helmi Bin, Saad Shahbudin Bin
ASEAN Journal on Science and Technology for Development
This research analysed the use of 3D Coral Photogrammetry (CP) and Coral Video Transect (CVT) images collected from SCUBA divers using a low-cost underwater action camera to examine the coral lifeform. A comparison was made between data sets obtained using both methods on nine transects with different coral lifeform compositions and percentage cover within an area of 4 × 7 m. The comparison of the statistical analysis for CPCe revealed that there were no significant differences (p < 0.05) between CP and CVT photos where dead corals (p = 0.006), sand (p = 0.011), and unknown (p = 0.002) are present. Additionally, the coral value (p = 0.131) between CP and CVT was not significant. CP was capable of producing prominent branching, massive, and plate coral morphology results. This suggests that survey methods using low-cost action cameras for 3D Coral Photogrammetry would yield appropriate results in terms of coral lifeform detection. Hypothetically, by improving camera quality, it will yield a higher accuracy of 3D coral images that are suitable for use in scientific research and management. Other benefits of using CP include the possibilities for future studies with 3D coral surveys using remotely operated vehicles, less field time, and 3D coral seabed information.
Problem Solving Of Isopropyl Alcohol – Water Azeotropic Characteristics Using Packed (Natural Zeolite) Bed Adsorber,
2020
Universiti Brunei Darussalam
Problem Solving Of Isopropyl Alcohol – Water Azeotropic Characteristics Using Packed (Natural Zeolite) Bed Adsorber, Prasakti Laras, Hartono Muhammad, Jati Pandu Prabowo, Setiaji Muhammad Fajar, Wirawan Sang Kompiang, Sudibyo Hanifrahmawan
ASEAN Journal on Science and Technology for Development
The adsorption kinetics of water from an azeotropic mixture of isopropyl alcohol and water using chemically activated natural zeolites with and without a binder (starch) was investigated. In addition, an investigation of the compressive strength of zeolite pellets was conducted to ascertain the performance of the adsorbent for long-term operation. Three parameters were applied in the zeolite-making process: the particle size of zeolite (20, 30, 50, and 80 mesh), the sintering temperature (550, 750, and 1,000°C), the compaction pressure (2, 4, and 6 tonnes), and the starch-to-zeolite weight ratio (0, 1:3, 1:5, and 1:7). Initial screenings indicated that the strongest …
An Analysis Of Crispr-Cas Gene Editing In Agriculture,
2020
University of Connecticut
An Analysis Of Crispr-Cas Gene Editing In Agriculture, Ashley Laliberte
Honors Scholar Theses
The CRISPR-Cas system is a promising form of gene editing, especially for the agriculture industry. The ability to make single-nucleotide edits within a gene of interest, without the need to introduce foreign DNA, is a powerful tool for designing healthier and more efficient crops and food animals. This system provides opportunity for increased nutritional value, decreased food waste, and more economically and environmentally sustainable food production. Though this biotechnology is facing mechanistic limitations due to off-target effects and inefficient homology-directed repair, vast improvements have already been made to improve its efficacy. The CRISPR-Cas system is already the most advanced form …
Inconceivable: An Analysis Of Assisted Reproductive Technology For The Church,
2020
Liberty University
Inconceivable: An Analysis Of Assisted Reproductive Technology For The Church, Mary Elizabeth Gresham
Senior Honors Theses
Infertility pushes the boundaries of emotional and physical health, which is why many couples inside and outside the church turn to Assisted Reproductive Technology (ART) for a solution. Despite what has seemed like silence from the Church, some individuals have braved the biological confusion and ethical dilemmas to evaluate the technology. Three major ethical viewpoints have emerged that each prioritize something over medical technology, namely community, order, or human dignity. This paper serves to educate pastors and church leaders on the ever-changing biology of ART as well as give voice to Christians that have spoken out on this issue. At …
Insight On Thermal Stability Of Magnetite Magnetosomes: Implications For The Fossil Record And Biotechnology,
2020
Universidade Federal do Rio de Janeiro
Insight On Thermal Stability Of Magnetite Magnetosomes: Implications For The Fossil Record And Biotechnology, Jefferson Cypriano, Mounib Bahri, Kassioge Dembele, Walid Baaziz, Pedro Leao, Dennis A. Bazylinski, Fernanda Abreu, Ovidiu Ersen, Marcos Farina, Jacques Werckmann
Life Sciences Faculty Research
Magnetosomes are intracellular magnetic nanocrystals composed of magnetite (Fe3O4) or greigite (Fe3S4), enveloped by a lipid bilayer membrane, produced by magnetotactic bacteria. Because of the stability of these structures in certain environments after cell death and lysis, magnetosome magnetite crystals contribute to the magnetization of sediments as well as providing a fossil record of ancient microbial ecosystems. The persistence or changes of the chemical and magnetic features of magnetosomes under certain conditions in different environments are important factors in biotechnology and paleomagnetism. Here we evaluated the thermal stability of magnetosomes in a temperature range between 150 and 500 °C subjected …
The Growing Threat Of Agroterrorism And Strategies For Agricultural Defense,
2020
Liberty University
The Growing Threat Of Agroterrorism And Strategies For Agricultural Defense, Alyssa Forrest
Senior Honors Theses
Due to the dynamic nature of human conflict, non-traditional terror tactics have evolved to undermine the socioeconomic stability of targeted societies. Considering the landscape in which terrorists operate, emphasis on more subversive methods of biological terror have become prominent in recent decades. Agroterrorism, or the use of plant pathogens to infect a nation’s cultivated crops, is an emerging topic due to its threat to global food security and economic stability. Although emergency preparedness objectives have been enacted at national, state, and even local levels, preemptive measures can no longer remain the sole responsibility of intelligence and law enforcement agencies. The …
Research And Review Of Polymethyl-Methacrylate Copolymers In The Development Of Enhanced Pharmaceutical Delivery Systems,
2020
Liberty University
Research And Review Of Polymethyl-Methacrylate Copolymers In The Development Of Enhanced Pharmaceutical Delivery Systems, Jake Dyess
Senior Honors Theses
The following thesis shall primarily explore the current research regarding the implementation of modifiable microscopic and nanoscopic polymer particles as novel pharmaceutical delivery systems. Polymethacrylate-based copolymers, such as EudragitTM, are sensitive to alterations in pH levels, becoming increasingly more soluble in response to an increase in pH. The selective solubility and modifiable nature of these polymers allows for greater flexibility of treatment options for patients (Patra et al., 2017). The wide range of functionality of polymethacrylate (PMA) may provide solutions to challenges relating to the current treatment and therapy of certain conditions. Research indicates that PMA based micro- …
Optimasi Teknik Sterilisasi Dan Induksi Tunas Tanaman Durian (Durio Zibethinus Murr) ‘Kamajaya’ Lokal Cimahi Secara In Vitro,
2020
Program Studi Agroteknologi, Fakultas Sains dan Teknologi, UIN Sunan Gunung Djati
Optimasi Teknik Sterilisasi Dan Induksi Tunas Tanaman Durian (Durio Zibethinus Murr) ‘Kamajaya’ Lokal Cimahi Secara In Vitro, Agung Rahmadi, Noladhi Wicaksana, Bambang Nurhadi, Erni Suminar, Siti Rakhmah Tenrisui Pakki, Syariful Mubarok
Jurnal Kultivasi
Durian ‘Kamajaya’ merupakan salah satu jenis durian lokal yang keberadaanya hampir punah sehingga perlu dilakukan konservasi, salah satunya yaitu dengan perbanyakan menggunakan kultur jaringan. Permasalahan yang muncul dalam kultur jaringan durian ini salah satunya adalah metode sterilisasi. Penelitian ini bertujuan untuk memperoleh metode sterilisasi yang tepat untuk perbanyakan tanaman durian ‘Kamajaya’ secara in vitro. Penelitian ini dilaksanakan di Laboratorium Kultur Jaringan Teknologi Benih, Fakultas Pertanian, Universitas Padjadjaran, sejak bulan Mei sampai September tahun 2019. Eksplan yang digunakan adalah tunas muda dari tanaman durian ‘Kamajaya’ yang berasal dari Cimahi. Sterilisasi untuk inisiasi dengan teknik kultur jaringan menggunakan kombinasi air mengalir, detergen, …
Role Of Microrna-208a As A Diagnostic And Prognostic Marker In Patients With St-Elevation Myocardial Infarction,
2020
The American University in Cairo
Role Of Microrna-208a As A Diagnostic And Prognostic Marker In Patients With St-Elevation Myocardial Infarction, Abou Bakr Mohamed Mohamed Hammad Salama
Theses and Dissertations
Acute Myocardial infarction (AMI) is the leading cause of death worldwide. Diagnosis of AMI depends on presenting symptoms, electrocardiogram (ECG), and cardiac troponins (cTns). Troponins are not good markers for patients presenting at the first hour of chest pain, so there is still a need for a marker that can be detected within the first hour of presentation, with high specificity and sensitivity, guide the medical decisions and give good insight on the expected prognosis. Previous studies reported miR-208a as a cardiac specific microRNA that can be detected in the plasma as early as 15 min of cardiac insult and …
Assessing Seed Handling Processes To Facilitate A Community-Engaged Approach To Regional Forest Restoration,
2020
Five RIvers Metroparks
Assessing Seed Handling Processes To Facilitate A Community-Engaged Approach To Regional Forest Restoration, Meredith Cobb, Michaela J. Woods, Ryan Mcewan
Biology Faculty Publications
In the Midwestern United States, there is a strong management impetus toward reforestation to replace trees lost to due to tree mortality and to establish forests on previously cleared properties. Here, we describe a public outreach and volunteer effort that aimed to generate positive, community-engaged restoration outcomes and report on a practical experiment involving seed handling. We obtained tree seeds that were donated by members of the community to local land managers. We evaluated the size of the seeds collected and tested the influence of common stratification media (none, sand and vermiculite) and seed size on germination success of three …
Optimization Of Xylanase Activity In The Presence Of Salts Of Different Metals For The Pulping Of Indigenous Non-Woody Raw Materials (Gossypium Arborium),
2020
Riphah International University, Faisalabad campus, Pakistan
Optimization Of Xylanase Activity In The Presence Of Salts Of Different Metals For The Pulping Of Indigenous Non-Woody Raw Materials (Gossypium Arborium), Muhammad Suleman, Abu Ul Hassan Faiz, Lariab Zahra Faiz
Journal of Bioresource Management
This study was conducted to optimize the process of degradation of agricultural wastes using xylanase by pulping. The cotton stalk was treated with enzyme and sugar with different concentration was obtained. The substrate concentration, incubation time, cofactors and activators on xylanase were investigated in the pulping and bleaching process. The study presents results that at pH 6.0 with time span (3, 6 and 9 hours) of incubation produced 56 mg/ml, 75 mg/ml and 112.5 mg/ml sugar respectively. While at pH 7.0 with time duration (3, 6 and 9 hours) of incubation 64 mg/ml, 75 mg/ml and 150 mg/ml of sugar …
Effect Of Homofermentative Inoculant On Fermentation Characteristics And Nutritive Values Of Corn Silage,
2020
Agripak Labs, Rawalpindi, Pakistan
Effect Of Homofermentative Inoculant On Fermentation Characteristics And Nutritive Values Of Corn Silage, Nafeesa Qudsia Hanif, Iffat Tahira, Nighat Sultana, Mujahid Hasan
Journal of Bioresource Management
An in vitro study was planned to assess the effects of a homofermentative microbial inoculant on the fermentation parameters and nutritive value of corn silage. The inoculant was applied at concentrations of 5x104cfu/g of forage (T1), 1x105cfu/g of forage (T2) 1.5x105cfu/g of forage (T3) and a negative control group (T0) without bacterial inoculant in three replicates each. At day 3, 7, 45 and 90 of the experiment individual buckets were opened to characterize the material, quick acidification, dry matter recovery, and aerobic stability of silage respectively. The temperature of the trial samples was 32.75±1.92 …
Calcium Enhances Plasmid Gene Transfection Efficiency In Jurkat Cells,
2020
University of Alabama in Huntsville
Calcium Enhances Plasmid Gene Transfection Efficiency In Jurkat Cells, V. S. S. Abhinav Ayyadevara
Research Horizons Day Posters
No abstract provided.
Transcriptome Analysis-Identified Long Noncoding Rna Crnde In Maintaining Endothelial Cell Proliferation, Migration, And Tube Formation,
2020
University of Nebraska - Lincoln
Transcriptome Analysis-Identified Long Noncoding Rna Crnde In Maintaining Endothelial Cell Proliferation, Migration, And Tube Formation, Matthew Moran, Xiao Cheng Zeng, Mohamed Sham Shihabudeen Haider Ali, Nishikant Wase, Nghi Nguyen, Weilong Yang, Chi Zhang, Concetta C. Dirusso, Xinghui Sun
Department of Biochemistry: Faculty Publications
Obesity is a leading risk factor for type-2 diabetes. Diabetes often leads to the dysregulation of angiogenesis, although the mechanism is not fully understood. Previously, long noncoding RNAs (lncRNAs) have been found to modulate angiogenesis. In this study, we asked how the expression levels of lncRNAs change in endothelial cells in response to excessive palmitic acid treatment, an obesitylike condition. Bioinformatics analysis revealed that 305 protein-coding transcripts were upregulated and 70 were downregulated, while 64 lncRNAs were upregulated and 46 were downregulated. Gene ontology and pathway analysis identified endoplasmic reticulum stress, HIF-1 signaling, and Toll-like receptor signaling as enriched after …
Molecular Differentiation Of Astragalus Species And Varieties From The Western United States: The Chloroplast Dna Bridge Between Evolution And Molecular Systematics,
2020
New Mexico State University
Molecular Differentiation Of Astragalus Species And Varieties From The Western United States: The Chloroplast Dna Bridge Between Evolution And Molecular Systematics, Marwa Neyaz, Daniel Cook, Rebecca Creamer
Poisonous Plant Research (PPR)
Locoweeds are the most widespread poisonous plant problem in the world and have been reported in the Western United States since the 1800s, causing tremendous losses in livestock. Consumption of locoweeds by grazing animals stimulates the neurological disease, locoism, characterized by weight loss, ataxia, and lack of muscular coordination. The name locoweed is used for Astragalus and Oxytropis species known to contain swainsonine, the toxic principle produced by the plant endophytic fungus Undifilum. Astragalus includes 2,500-3,000 species and many varieties that have almost identical morphological characteristics that overlap among species, leading to improper identification. Therefore, the aim of this study …
