Mutagenesis Of The Btea Gene Encoding A Bordetella Virulence Protein,
2020
Eastern Washington University
Mutagenesis Of The Btea Gene Encoding A Bordetella Virulence Protein, Xiaolei Mao
2020 Symposium Posters
Bordetella Type III Secretion System Effector A (BteA) is a virulence protein found in members of the genus Bordetella which include important pathogens of humans and other mammals. Bordetella pertussis is a causative agent of the whooping cough, a highly contagious respiratory disease that is especially dangerous, and sometimes deadly, for infants. The BteA protein appears to be an important factor in the ability of these pathogens to cause disease, as it leads to rapid killing of a wide range of mammalian cells. The aim of this project is to determine which regions of the DNA are important for mediating …
Additive Modulation Of Dna-Dna Interactions By Interstitial Ions,
2020
Hefei University / George Washington University
Additive Modulation Of Dna-Dna Interactions By Interstitial Ions, Wei Meng, Raju Timsina, Abby Bull, Kurt Andresen, Xiangyun Qiu
Physics and Astronomy Faculty Publications
Quantitative understanding of biomolecular electrostatics, particularly involving multivalent ions and highly charged surfaces, remains lacking. Ion-modulated interactions between nucleic acids provide a model system in which electrostatics plays a dominant role. Using ordered DNA arrays neutralized by spherical cobalt3+ hexammine and Mg2+ ions, we investigate how the interstitial ions modulate DNA-DNA interactions. Using methods of ion counting, osmotic stress, and x-ray diffraction, we systematically determine thermodynamic quantities, including ion chemical potentials, ion partition, DNA osmotic pressure and force, and DNA-DNA spacing. Analyses of the multidimensional data provide quantitative insights into their interdependencies. The key finding of this study is that …
Integrated Unicellular/Filamentous Algal Production, Harvesting And Remediation System,
2020
Center for Metabolic Disease, Ordway Research Institute
Integrated Unicellular/Filamentous Algal Production, Harvesting And Remediation System, Paul N. Black, James Allen, Timothy J Nicodemus
Department of Biochemistry: Faculty Publications
A method of removing nitrogen-bound nitrate from at least one of groundwater, surface water, or waste water is disclosed. The method includes providing contaminant-containing water from groundwater, surface water, and/or waste water sources. The method further includes adding the contaminant-containing water to an algal photobioreactor system. The method further includes adding an alga culture to the alga photobioreactor system. The method further includes adjusting temperature, CO2 concentration, pH, light wavelength, and/or light intensity in the algal photobioreactor system to optimize the growth of the algea. The method further includes separating the aglae from the water and harvesting algal biomass.
Minding The Gaps In Regulation Of Do-It-Yourself Biotechnology,
2020
University of Florida
Minding The Gaps In Regulation Of Do-It-Yourself Biotechnology, Barbara J. Evans
DePaul Journal of Health Care Law
No abstract provided.
Recovery And Purification Of Glycerine As By-Product From Philippine Coconut Methyl Ester,
2020
Universiti Brunei Darussalam
Recovery And Purification Of Glycerine As By-Product From Philippine Coconut Methyl Ester, Briones Annabelle V.
ASEAN Journal on Science and Technology for Development
The study is about the recovery and purification of glycerine as a by-product from coconut methyl ester production in the Philippines. The aqueous layer produced from settling or phase splitting of the methyl ester after the transesterification process was subjected to various treatments like acidification, neutralization, concentration in vacuo in order to get back the crude glycerine. The crude glycerine obtained from the laboratory and scale-up process conformed with the specification set by the British standard for crude glycerine. The recovered glycerine is composed of: 84.92%, glycerine; 8.03%, ash; 4.72%, H20; 2.32%, MONG. Further distillation yielded a refined glycerine that …
Expression Analyses Of Hippocampal And Cortical Proteins In A Rat Model For Alzheimer’S Disease,
2020
CUNY Hunter College
Expression Analyses Of Hippocampal And Cortical Proteins In A Rat Model For Alzheimer’S Disease, Rangon Islam
Theses and Dissertations
Currently, Alzheimer’s disease (AD) has no cure. Using a rat AD model, we identified aberrantly expressed proteins during pre-pathology as potential biomarkers. The expression of certain biomarkers was reversed by diazoxide, a repurposed hypertension drug. These results suggest that drug repurposing at an early stage of AD has therapeutic potential.
Design Of A 3d Printed Bioreactor For Bone Cancer Research,
2020
South Dakota State University
Design Of A 3d Printed Bioreactor For Bone Cancer Research, Brooklyn K. Vanderwolde, Katelyn Hillson
The Journal of Undergraduate Research
Bone cancer is an aggressive disease and has peak occurrence during physiological stimulation of growth and aging. Astronauts who undergo long-term space missions also acquire an increased risk of bone tissue degeneration and cancer. Few in-vitro models currently exist capable of reproducing the complex microenvironment of bone tissue to support multicellular activity in a three-dimensional structure. This limits the ability to understand disease progression and develop suitable treatment strategies. Therefore, developing a bone tissue model and an associated bioreactor is critical to understand the risks associated with cancer progression and improve treatment and preventions related to those risks. The goal …
Circulating Micrornas In Neuromuscular Disorders,
2020
The American University in Cairo
Circulating Micrornas In Neuromuscular Disorders, Nahla Mousa
Theses and Dissertations
Identification of the mutations and genetic causes of the muscular diseases is performed for the muscular dystrophies, atrophies and other genetic diseases using current molecular high throughput techniques that precisely allocate the defected genes and detect the aberration in the expressed proteins responsible for any phenotype. For example, more than 2000 mutations in the DMD gene were identified in Duchenne Muscular Dystrophy patients or the milder form of the disease Becker Muscular Dystrophy. Detection of different mutation in the same gene has critical role as biomarkers in disease diagnosis, since the severity of the symptoms is usually associated with the …
Using A Novel Cell- Penetrating Peptide Technology To Induce Pluripotency,
2020
Kennesaw State University
Using A Novel Cell- Penetrating Peptide Technology To Induce Pluripotency, Laura-Maria Oja
Master of Science in Chemical Sciences Theses
Somatic stem cells have been used as a regenerative medicine tool to treat diseases like leukemia for decades, but they are limited in their ability to self-renew and differentiate. A better alternative, induced pluripotent stem cells (iPSCs), has the ability to generate cells from all three germ layers and avoid tissue rejection as well as bypass the ethical concerns related to embryonic stem cells. In 2006, Takahashi and Yamanaka discovered that only four genes were required to induce pluripotency: Oct4, Sox2, Klf4, and c-Myc (OSKM). Since then, pluripotency has been induced with OSKM most effectively by transfection or transduction. However, …
Understanding Covid-19 With Single Cell Immune Profiling,
2020
University of San Francisco
Understanding Covid-19 With Single Cell Immune Profiling, Alex Palacio
Creative Activity and Research Day - CARD
Scientists all around the world are coming together for a single goal -- to find the cure for COVID-19; the virus that has sent millions of people worldwide to hospitals and hundreds of thousands of people to their death. While scientists are trying to uncover the mysteries of the virus, 10X Genomics product lines are able to help scientists achieve their goal. With the Single Cell Immune Profiling, scientists around the world are able to study the immune response and antigen specificity of the virus. The Immune Profiling product line is able to study millions of T-Cells and B-Cells on …
Chemoenzymatic Study Of Coa-Linked Rna In Bacteria,
2020
The University of Southern Mississippi
Chemoenzymatic Study Of Coa-Linked Rna In Bacteria, Krishna Sapkota
Dissertations
The ability of RNA to store genetic information and to catalyze biochemical transformations led to the speculation of the existence of RNA world before the evolution of contemporary ribonucleoprotein (RNP) world. Recent discovery of RNA molecules containing metabolic cofactors including coenzyme A and its various thioesters at their 5’ end further supported the RNA world hypothesis as these CoA-linked RNA molecules could be the molecular fossils with very ancient origin. As both RNA and Coenzyme A are believed to have co-existed since last universal common ancestor (LUCA) or even before, the CoA-RNA conjugates in current biology may reveal fundamental molecular …
Optimization Of Rrna Depletion By Testing Various Kits,
2020
University of San Francisco
Optimization Of Rrna Depletion By Testing Various Kits, Alyssa Sancio
Creative Activity and Research Day - CARD
RNA sequencing (RNAseq) can be used to compare gene expression between different groups, identify mutations, and confirm gene knockouts. In RNAseq, RNA is extracted and built into a sequenceable library to analyze the transcriptome. Ribosomal RNA (rRNA) makes up roughly 90% of the total RNA extracted and does not provide useful transcriptome information, thus it must be removed in order to perform transcriptional analysis. At MBP Titan, the protocol used KAPA’s RiboErase Depletion kit using custom methanotroph oligonucleotides to specifically bind to rRNA for depletion. On average, the amount of rRNA remaining in the libraries ranged from 10-30%. Several depletion …
Plasmid Dna Upstream Manufacturing Process Development,
2020
University of San Francisco
Plasmid Dna Upstream Manufacturing Process Development, Shaquil Janif
Creative Activity and Research Day - CARD
The need to develop a robust method to produce large quantities of plasmid DNA has become vital due to the strides made in the field of gene therapy. More and more biopharmaceutical companies are starting to produce gene therapeutics to treat diseases at the molecular level. While the technology has been proven to work and success at treating disease using gene therapeutics have been made in clinical trials, a method to produce large quantities of DNA still remains a challenge. In this presentation, I will go over some of the current challenges associated with producing high copy numbers of plasmid …
Antibody Binding Screens Using Biolayer Interferometry (Bli) And Surface Plasmon Resonance (Spr),
2020
University of San Francisco
Antibody Binding Screens Using Biolayer Interferometry (Bli) And Surface Plasmon Resonance (Spr), Kerul Patel
Creative Activity and Research Day - CARD
BLI and SPR are both label-free technologies being used to measure biomolecular interactions in real-time. Distributed Bio uses computational immunology techniques to generate antibody libraries against a target and bio-panning to identify and select clones that best interact with the target. The interaction is further tested using BLI and SPR technology platforms that enable determination of binding kinetics such as association and dissociation rates also known as on-rates and off-rates. Analysis of the on-rate and off-rate data is further used to determine and report the binding affinity of the target to the antibody clones which are then delivered to the …
Hexadecane Petroleum, And Biofuel Utilization In Marine Bacteria Isolated From Ballast Tanks,
2020
University of Texas Permian Basin
Hexadecane Petroleum, And Biofuel Utilization In Marine Bacteria Isolated From Ballast Tanks, Alex Yashchenko
Graduate Theses
This study characterized the growth of bacteria isolated from ballast tank fluids in hexadecane, petroleum, plant, and algae-derived fuels. The study was performed to explore the capacity of ballast tank isolates to survive and grow within fuels that may be stored within ballast tanks. Results of the hexadecane analysis indicated that most isolates had higher viable cell counts in media supplemented with hexadecane. Members of Alteromonas, Pseudoalteromonas, and a single Brevundimonas species had viable cell counts that were one or several orders ofmagnitude greater than that of controls. Results offuel analysis indicated higher viable cell counts in pure JP-5 and …
Cloning And Expression Of Hydra Innexin 2, A Gap Junction Protein Required For Coordinated Contraction Of The Body Column,
2020
Buffalo State College
Cloning And Expression Of Hydra Innexin 2, A Gap Junction Protein Required For Coordinated Contraction Of The Body Column, Ashley O'Brien
Biology Theses
In invertebrates gap junctions are formed by the innexin family of proteins. Remarkably, the genome of Hydra magnipapillata contains 17 innexin genes. This study focused on Hydra innexin-2 (h-Inx2) which is expressed in nerve cells and plays a role in contraction of the body column. The gene sequence of H-Inx2 was obtained from the National Center for Biotechnology Information (NCBI), the gene was synthesized externally and transferred to a vector suitable for expression in Xenopus oocytes (pcDNA3.1 CT-GFP TOPO). The TOPO CT-GFP vector includes a priming site for RNA polymerase which allows in vitro preparation of RNA. Another advantage is …
A Crispr-Based Approach To Identify Host Factors Influencing Aav Production,
2020
University of San Francisco
A Crispr-Based Approach To Identify Host Factors Influencing Aav Production, Bryant Lowry
Creative Activity and Research Day - CARD
Historically, the Adeno-Associated Virus (AAV) has been used as a vector for gene therapies due to its low pathogenicity, no known associated diseases, and clinical validity. However, transitioning from in-vitro AAV experiments to in-vivo prove challenging for both scalability and potency. It has been previously characterized that some host factors in human cells influence the packaging capabilities of the AAV vector. Therefore, with the assistance of Encoded Therapeutics, this project aims to validate previous efforts to identify human host factors that influence AAV production in cells with the intent to understand these mechanisms for therapeutic manufacturing benefit to lower AAV …
Project Manager: Antibody Discovery At Distributed Bio,
2020
University of San Francisco
Project Manager: Antibody Discovery At Distributed Bio, Pedro I. Justiniano
Creative Activity and Research Day - CARD
Antibody discovery platforms have opened a new frontier in therapeutic development. Distributed Bio has built antibody libraries that produce computationally optimized antibodies pre-engineered with therapeutically desirable traits such as humanness, high CDRH3 diversity, thermostability, and other molecular scaffolding proven to pass clinical trial requirements. One of their most popular libraries is the Super Human 2.0 library. This library analyzes billions of antibodies to find the most suited antibody to bind to a specific antigen target. / As a Project Manager intern at Distributed Bio, I have been helping to manage projects of different companies to provide them with a product …
Assessing The Influence Of Riparian Invasion By The Shrub Lonicera Maackii On Terrestrial Subsidies To Headwater Streams,
2020
University of Dayton
Assessing The Influence Of Riparian Invasion By The Shrub Lonicera Maackii On Terrestrial Subsidies To Headwater Streams, Erin C. Rowekamp, Julia I. Chapman, Ryan Mcewan
Biology Faculty Publications
Invasion of Amur honeysuckle (Lonicera maackii) into riparian areas of headwater streams creates strong potential for alterations of terrestrial subsidies that may drive bottom-up effects on aquatic biota. In this study, we analyzed effects of L. maackii on terrestrial subsidies in stream sites that represented a gradient of invasion intensity in temperate deciduous forests of southwestern Ohio (USA). Leaf litter biomass, terrestrial and aquatic fine woody debris (0.5–9.9 cm diameter) volume and count, and terrestrial and aquatic coarse woody debris (>9.9 cm diameter) volume were analyzed. Based on previous research, we hypothesized that sites with a higher …
Direct Evidence Of Missing Mepsps Using Ca2-Sensor Imaging,
2020
University at Albany, State University of New York
Direct Evidence Of Missing Mepsps Using Ca2-Sensor Imaging, Petar Gajic
Biological Sciences
Following an action potential in the presynaptic neuron there is evoked release of neurotransmitter into the synapse which activates ionotropic transmembrane receptors on the postsynaptic membrane that cause depolarizations in voltage that get recorded as excitatory postsynaptic potentials (EPSPs). In the absence of an action potential there is spontaneous release of neurotransmitter that postsynaptically gets recorded as miniature excitatory postsynaptic potentials (mEPSPs). According to the quantal hypothesis, postulated by Bernard Katz, the mEPSPs are allor- none changes in potential caused by a single quantum of neurotransmitter, which when added up create EPSPs. Following studies have found that these two modes …
