Effects Of Diesel And Biodiesel Blends On Engine Performance And Efficiency,
2010
University of Arkansas, Fayetteville
Effects Of Diesel And Biodiesel Blends On Engine Performance And Efficiency, Christopher Hunt, Donald Johnson, Don Edgar
Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences
Tests were conducted during the summer of 2009 on a John Deere 3203 diesel tractor to determine differences in specific fuel consumption (sfc), power take-off (PTO) torque (Nm), and PTO power (kW), between ultralow sulfur No. 2 Diesel (D2), 20% biodiesel (B20), 50% biodiesel (B50), and100% biodiesel (B100). Four 1-hr tests were conducted with D2, while three 1-hr tests were conducted with B20, B50, and B100. The results indicated that there was no significant (p < 0.05) difference between D2 and B20 for power or torque. Fueling with B50 resulted in significantly lower power and torque than fueling with D2 or B20, but significantly higher power and torque than fueling with B100. There were significant differences between each fuel in sfc; as the biodiesel blend increased, sfc also increased. Based on these data, B20 appears to be the optimal biodiesel blend for this and similar compact utility tractors since fueling with B20 resulted in no significant loss in power or torque (compared to D2) and only a slight increase in fuel consumption.
Evaluating The Target Pipeline In A Pharmaceutical Acquisition,
2010
Ursinus College
Evaluating The Target Pipeline In A Pharmaceutical Acquisition, Daniel Vass
Business and Economics Honors Papers
Many firms in the pharmaceutical industry turn to acquisitions when faced with gaps in their drug development pipelines and patent expirations as an alternative to making long-term investments in internal research and development. Investors are generally negative on this strategy, and upon the announcement of a pharmaceutical acquisition the stock of the acquiring firm often drops. This decline in share price creates an opportunity for the investor who can identify the characteristics of a target firm that increase the probability that the transaction will ultimately be a success, as measured by the subsequent appreciation in the acquirer's stock. It is …
Unstandard Standardization: The Case Of Biology,
2010
Duke Law School
Unstandard Standardization: The Case Of Biology, Arti K. Rai
Faculty Scholarship
How applicable are the approaches adopted by information and communication technology standards-setting organizations to biological standards? Most engineering-based industries construct products from standard, well understood components. By contrast, despite the early attachment of the moniker “genetic engineering” to biotechnology, standardization in the biological sciences has been relatively rare.
Quantitative Pcr Assay For Mycobacterium Pseudoshottsii And Mycobacterium Shottsii And Application To Environmental Samples And Fishes From The Chesapeake Bay,
2010
Old Dominion University
Quantitative Pcr Assay For Mycobacterium Pseudoshottsii And Mycobacterium Shottsii And Application To Environmental Samples And Fishes From The Chesapeake Bay, D. T. Gauthier, K. S. Reece, J. Xiao, M. W. Rhodes, H. I. Kator, R. J. Latour, C. F. Bonzek, J. M. Hoenig, W. K. Vogelbein
Biological Sciences Faculty Publications
Striped bass (Morone saxatilis) in the Chesapeake Bay are currently experiencing a very high prevalence of mycobacteriosis associated with newly described Mycobacterium species, Mycobacterium pseudoshottsii and M. shottsii. The ecology of these mycobacteria outside the striped bass host is currently unknown. In this work, we developed quantitative real-time PCR assays for M. pseudoshottsii and M. shottsii and applied these assays to DNA extracts from Chesapeake Bay water and sediment samples, as well as to tissues from two dominant prey of striped bass, Atlantic menhaden (Brevoortia tyrannus) and bay anchovy (Anchoa mitchilli). Mycobacterium …
Estimation Of The Product Yield And Collection Efficiency Of Peripheral Blood Stem Cell Harvesting Using Peripheral Blood Cd34[+] Cells Quantification.,
2010
Universiti Malaya
Estimation Of The Product Yield And Collection Efficiency Of Peripheral Blood Stem Cell Harvesting Using Peripheral Blood Cd34[+] Cells Quantification., Habsah Aziz
Student Works (2010-2019)
Peripheral blood stem cells (PBSC) have been used for transplantation since the early 1990s. CD34+ has been used as a marker of hematopoietic stem cells to determine the optimal timing for PBSC harvesting. However, a wide ranges of CD34+ cells cut-off values from 8/ul to 66/ul have been used to initiate PBSC harvesting in autologous patients. We performed here a study to determine the peripheral blood CD34+ cells cut-off value for the prediction of stem cell yield of 2 x106 CD34+ cells/kg body weight patients, to study the correlation between peripheral blood CD34+ cells and stem cell yield, to study …
Diversity And Antioxidant Activity Of Trametes Fr. In Malaysia.,
2010
Universiti Malaya
Diversity And Antioxidant Activity Of Trametes Fr. In Malaysia., Noraswati Mohd Nor Rashid
Student Works (2010-2019)
Research on antioxidant properties as a natural antioxidant, particularly in polypores, have been widely done since the past decade. It is known that antioxidant capacity is essential in protecting organisms and cells from damage brought on by oxidative which is believed to be the cause for ageing and degenerative diseases. In this study, biodiversity and antioxidant activity of Malaysian Trametes spp. which is from the group of polypores were investigated. Polypores were collected in Peninsular Malaysia forests from the year 2006 until 2007. The species were identified by taxonomic keys based on macromorphology and micromorphology analysis. Trametes hirsuta, T. pocas, …
Identification And Characterization Of Oxalate Oxidoreductase,
A Novel Thiamine Pyrophosphate-Dependent 2-Oxoacid
Oxidoreductase That Enables Anaerobic Growth On Oxalate,
2010
University of Michigan, Ann Arbor
Identification And Characterization Of Oxalate Oxidoreductase, A Novel Thiamine Pyrophosphate-Dependent 2-Oxoacid Oxidoreductase That Enables Anaerobic Growth On Oxalate, Elizabeth Pierce, Donald F. Becker, Stephen W. Ragsdale
Department of Biochemistry: Faculty Publications
Moorella thermoacetica is an anaerobic acetogen, a class of bacteria that is found in the soil, the animal gastrointestinal tract, and the rumen. This organism engages the Wood-Ljungdahl pathway of anaerobic CO2 fixation for heterotrophic or autotrophic growth. This paper describes a novel enzyme, oxalate oxidoreductase (OOR), that enables M. thermoacetica to grow on oxalate, which is produced in soil and is a common component of kidney stones. Exposure to oxalate leads to the induction of three proteins that are subunits of OOR, which oxidizes oxalate coupled to the production of two electrons and CO2 or bicarbonate. Like …
Functional Hybrid Rubisco Enzymes With Plant Small
Subunits And Algal Large Subunits
Engineered Rbcs Cdna For Expression In Chlamydomonas,
2010
University of Nebraska-Lincoln
Functional Hybrid Rubisco Enzymes With Plant Small Subunits And Algal Large Subunits Engineered Rbcs Cdna For Expression In Chlamydomonas, Todor Genkov, Moritz Meyer, Howard Griffiths, Robert J. Spreitzer
Department of Biochemistry: Faculty Publications
There has been much interest in the chloroplast-encoded large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) as a target for engineering an increase in net CO2 fixation in photosynthesis. Improvements in the enzyme would lead to an increase in the production of food, fiber, and renewable energy. Although the large subunit contains the active site, a family of rbcS nuclear genes encodes the Rubisco small subunits, which can also influence the carboxylation catalytic efficiency and CO2/O2 specificity of the enzyme. To further define the role of the small subunit in Rubisco function, small subunits from spinach, Arabidopsis, …
Increased Perfusion And Angiogenesis In A Hindlimb Ischemia Model With Plasmid Fgf-2 Delivered By Noninvasive Electroporation,
2010
Old Dominion University
Increased Perfusion And Angiogenesis In A Hindlimb Ischemia Model With Plasmid Fgf-2 Delivered By Noninvasive Electroporation, B. Ferraro, Y. L. Cruz, M. Baldwin, D. Coppola, R. Heller
Bioelectrics Publications
Gene therapy approaches delivering fibroblast growth factor-2 (FGF-2) have shown promise as a potential treatment for increasing blood flow to ischemic limbs. Currently, effective noninvasive techniques to deliver plasmids encoding genes of therapeutic interest, such as FGF-2, are limited. We sought to determine if intradermal injection of plasmid DNA encoding FGF-2 (pFGF) followed by noninvasive cutaneous electroporation (pFGFE+) could increase blood flow and angiogenesis in a rat model of hindlimb ischemia. pFGFE+ or control treatments were administered on postoperative day 0. Compared to injection of pFGF alone (pFGFE-), delivery of pFGFE+ significantly increased FGF-2 expression for 10 days. Further, the …
Low Cost Production Of Proinsulin In Tobacco And Lettuce Chloroplasts For Injectable Or Oral Delivery Of Functional Insulin And,
2010
University of Central Florida
Low Cost Production Of Proinsulin In Tobacco And Lettuce Chloroplasts For Injectable Or Oral Delivery Of Functional Insulin And, Diane Burberry
Electronic Theses and Dissertations
Current treatment for type I diabetes includes delivery of insulin via injection or pump, which is highly invasive and expensive. The production of chloroplast-derived proinsulin should reduce cost and facilitate oral delivery. Therefore, tobacco and lettuce chloroplasts were transformed with the cholera toxin B subunit fused with human proinsulin (A, B, and C peptides) containing three furin cleavage sites (CTB-PFx3). Transplastomic lines were confirmed for site-specific integration of transgene and homoplasmy. Old tobacco leaves accumulated proinsulin up to 47% of total leaf protein (TLP). Old lettuce leaves accumulated proinsulin up to 53% TLP. Accumulation was so stable that up to …
Acetazolamide-Induced Decrease Of Apical Fluid Flow In Choroid Plexus Is Independent Of The Concomitant Changes In Aquaporin-1 Expression,
2010
University of Central Florida
Acetazolamide-Induced Decrease Of Apical Fluid Flow In Choroid Plexus Is Independent Of The Concomitant Changes In Aquaporin-1 Expression, Pouya Alexander Ameli
Electronic Theses and Dissertations
Acetazolamide (AZA), the only drug approved for treatment of hydrocephalus, is effective in only 25-30% of patients while its effect on fluid flow in the choroid plexus (CP) is unknown. The drug reversibly inhibits Aquaporin 4 (AQP4), the most highly expressed „water pore‟ in the brain, and it is postulated that it reduces cerebrospinal fluid (CSF) production by modulating AQP1 (mostly found in the apical membrane of the CP). In this study, we sought to elucidate the effect of AZA on AQP1 and fluid flow in CP. Primary CP culture from p10 Sprague-Dawley rats and TRCSF-B cell line were grown …
Electric Pulses To Prepare Feeder Cells For Sustaining And Culturing Of Undifferentiated Embryonic Stem Cells,
2010
Old Dominion University
Electric Pulses To Prepare Feeder Cells For Sustaining And Culturing Of Undifferentiated Embryonic Stem Cells, Lauren M. Browning, Tao Huang, Xiao-Hong Nancy Xu
Chemistry & Biochemistry Faculty Publications
Current challenges in embryonic-stem-cell (ESC) research include inability of sustaining and culturing of undifferentiated ESCs over time. Growth-arrested feeder cells are essential to the culture and sustaining of undifferentiated ESCs, and they are currently prepared using gammaradiation and chemical inactivation. Both techniques have severe limitations. In this study, we developed a new, simple and effective technique (pulsed-electric-fields, PEFs) to produce viable growth-arrested cells (RTS34st) and used them as high-quality feeder cells to culture and sustain undifferentiated zebrafish ESCs over time. The cells were exposed to 25 sequential 10- nanosecond-electric-pulses (10nsEPs) of 25, 40 and 150 kV/cm with 1s pulse interval, …
Studies On The Bioactive Properties Of Selected Plant And Fermented Extracts.,
2010
Universiti Malaya
Studies On The Bioactive Properties Of Selected Plant And Fermented Extracts., Saravana Kumar Sinniah
Student Works (2010-2019)
The aim of this study was to isolate protein/peptide and secondary metabolites from plant and fermented sources. The extracts were used to test for antimicrobial and antioxidant properties. In the current study protein/peptide extracts of Allium sativum showed promising results with a 15mm inhibition zone against Escherichia coli, 28mm against Staphylococcus aureus, 16.3mm against Bacillus cereus and 9mm against Pseudomonas aeruginosa. The minimum bacteriocidal concentration (MBC) results showed that Allium sativum protein/peptide extract is only bacteriostatic and not bacteriocidal. Andrographis paniculata showed the most promising result for the ethanolic extracts with an inhibition zone of 14mm against B. cereus, 11.5mm …
Purification And Analysis Of Bacterial-And Plant-Derived Scfv Antibodies For The Detection Of Cucumber Mosaic Virus,
2010
Universiti Malaya
Purification And Analysis Of Bacterial-And Plant-Derived Scfv Antibodies For The Detection Of Cucumber Mosaic Virus, Wong C.F.Y.
Student Works (2010-2019)
abstract N/A
Antibactertial Property And Toxcity Study Of Medicinal Plant Tinospora Crispa L.,
2010
Universiti Malaya
Antibactertial Property And Toxcity Study Of Medicinal Plant Tinospora Crispa L., Altammar Khadijah Ahmed Y.
Student Works (2010-2019)
Tinospora crispa is a medically important herb used for centuries in traditional medicine for the treatment of various ailments. The aim of this study was to investigate the safety of the aqueous and alcoholic extract of Tinospora crispa by determining its toxicological effects after acute administration in rats, and to investigate the antibacterial activity of both extracts. Disc diffusion and broth dilution methods were carried out in determining the antibacterial effects of the plant extract on the pure cultures of clinical isolates of 8 MRSA and S. aureus ATCC strain. The disc-diffusion method of the ethanolic extracts showed inhibition zone …
Biolistic Transformation Of Oil Palm Using The Phosphomannose Isomerase (Pmi) Gene As The Positive Selectable Marker.,
2010
Universiti Malaya
Biolistic Transformation Of Oil Palm Using The Phosphomannose Isomerase (Pmi) Gene As The Positive Selectable Marker., Bahariah Bohari
Student Works (2010-2019)
The selectable marker system based on the Escherichia coli phosphomannose isomerase (pmi) gene was adapted for genetic transformation of oil palm. This system makes use of the pmi gene that encodes phosphomannose isomerase, which converts mannose-6-phosphate to fructose-6-phosphate and uses mannose as the selection agent. This is to anticipate the future requirement of using non-antibiotic resistance genes for the commercialization of transgenic oil palm. The use of antibiotic or herbicide-based selection systems has caused much public concern due to inadequate knowledge of the agents’ impact on the environment and on human health. In this study, four transformation vectors, namely pMI3, …
Cytotoxic And Anti-Human Papillomavirus Activites In Selected Vegetables.,
2010
Universiti Malaya
Cytotoxic And Anti-Human Papillomavirus Activites In Selected Vegetables., Maizatul Azmah Ab. Latiff
Student Works (2010-2019)
An appropriate way to control cancer is chemoprevention as a large number of compounds have been observed to prevent the occurrence of cancer and variety of mechanisms exists for producing such protective effects. Numerous epidemiological studies have shown that diets rich in vegetables are associated with a decreased risk of cancer. Chemoprevention involves the use of natural or laboratory made substances to prevent cancer or reduce risk cancer.The E6 oncoprotein from oncogenic Human papillomaviruses (HPVs) has exhibit functions in tumorigenesis, regulation of transcriptions and apoptosis. E6 product from oncogenic viral type like HPV 16 can bind the tumor suppressor p53 …
Antagonistic Activity Of Selected Marine-Derived Streptomyces Spp. Against Plesiomonas Shigelloides.,
2010
Universiti Malaya
Antagonistic Activity Of Selected Marine-Derived Streptomyces Spp. Against Plesiomonas Shigelloides., Angelyn Melaya Kloni
Student Works (2010-2019)
In this study, primary and secondary screenings were conducted to evaluate the antibacterial and anti-yeast potential of the eleven selected marine-derived Streptomyces spp., particularly against P. shigelloides. The strains were categorized into three colour groups for morphological grouping according to the colour of aerial mycelium; namely grey, yellow green, and white colour groups. Diffusible pigments were produced by eight of the strains tested. Strain(s) from grey and white with red diffusible pigments colour groups displayed strong antibacterial activity. The yeasts tested were resistant to the bioactive metabolite(s) produced by some of the Streptomyces spp. which had shown high activity against …
Electroporation-Mediated Delivery Of A Naked Dna Plasmid Expressing Vegf To The Porcine Heart Enhances Protein Expression,
2010
Old Dominion University
Electroporation-Mediated Delivery Of A Naked Dna Plasmid Expressing Vegf To The Porcine Heart Enhances Protein Expression, W. G. Marshall Jr., B. A. Boone, J. D. Burgos, S. I. Gografe, M. K. Baldwin, M. L. Danielson, M. J. Larson, D. R. Caretto, Y. Cruz, B. Ferraro, L. C. Heller, K. E. Ugen, M. J. Jaroszeski, R. Heller
Bioelectrics Publications
Gene therapy is an attractive method for the treatment of cardiovascular disease. However, using current strategies, induction of gene expression at therapeutic levels is often inefficient. In this study, we show a novel electroporation (EP) method to enhance the delivery of a plasmid expressing an angiogenic growth factor (vascular endothelial growth factor, VEGF), which is a molecule previously documented to stimulate revascularization in coronary artery disease. DNA expression plasmids were delivered in vivo to the porcine heart with or without coadministered EP to determine the potential effect of electrically mediated delivery. The results showed that plasmid delivery through EP significantly …
Untreated And Enzyme-Modified Bovine Whey Products Reduce Association Of Salmonella Typhimurium, Escherichia Coli O157:H7 And Cronobacter Malonaticus (Formerly Enterobacter Sakazakii) To Caco-2 Cells,
2010
Technological University Dublin
Untreated And Enzyme-Modified Bovine Whey Products Reduce Association Of Salmonella Typhimurium, Escherichia Coli O157:H7 And Cronobacter Malonaticus (Formerly Enterobacter Sakazakii) To Caco-2 Cells, Rachel Halpin, D.B. Brady, E.D. O’Riordan, M. O’Sullivan
Articles
Adhesion of a micro-organism to a cell surface is often considered to be the first step in pathogenesis. Inhibiting this process may have therapeutic effects in vivo. This study investigates the inhibitory effects of various bovine whey products on the association of Salm. Typhimurium, E. coli O157:H7 and C. malonaticus (formerly Enterobacter sakazakii) to the human CaCo-2 cell line. Invasion of CaCo-2 cells by Salm. Typhimurium and C. malonaticus was also examined.
