Systems Biology Commons^™

Larval Performance Of A Major Forest Pest On Novel Hosts And The Effect Of Stressors, Donnie Lee Peterson, Don Cipollini

Biological Sciences Faculty Publications

Novel hosts lacking a coevolutionary history with herbivores can often support improved larval performance over historic hosts; e.g., emerald ash borer [Agrilus planipennis (Fairmaire) Coleoptera: Buprestidae] on North American ash (Fraxinus spp.) trees. Whether trees are novel or ancestral, stress on plants increases emerald ash borer preference and performance. White fringetree [Chionanthus virginicus (L.) Lamiales: Oleaceae] and olive [Olea europaea (L.) Lamiales: Oleaceae] are closely related non-ash hosts that support development of emerald ash borer to adulthood, but their relative suitability as hosts and the impact of plant stress on larval success has not been well …

On The Potential For Saturated Buffers In Northwest Ohio To Remediate Nutrients From Agricultural Runoff, Stephen J. Jacquemin, Gregory J. Mcglinch, Theresa Dirksen, Angela Clayton

Biological Sciences Faculty Publications

Nutrient loading from nonpoint source runoff in the Midwest has emerged as one of the largest threats to water quality as the frequency of harmful algal blooms, hypoxic zones, and issues associated with human-resource interactions have risen abruptly over the past several decades. In this study, a saturated buffer ~500 m in length located in the western basin of the Lake Erie watershed was evaluated for its potential to reduce edge of field runoff and nutrient loading. Saturated buffers reduce runoff by routing subsurface tile drainage water into the riparian zone, providing an opportunity for drainage volume as well as …

The Role Of Manganese In Streptococcus Sanguinis, Tanya M. Puccio

Theses and Dissertations

Streptococcus sanguinis is primarily associated with oral health as a commensal bacterium. As an opportunistic pathogen, S. sanguinis is capable of colonizing heart valve vegetations, leading to the disease infective endocarditis. Previous studies from our lab have identified the high-affinity manganese transporter SsaACB as important for endocarditis virulence. The impact that manganese depletion has on S. sanguinis had never been evaluated and a secondary manganese transporter has not been identified. Thus, we employed the use of a fermentor to control large-scale growth over time and depleted manganese in an ΔssaACB mutant using a metal chelator, EDTA. The changes in …

The Origin And Evolution Of Flower Characters In Angiosperms With A Case Study On The Genetic Basis Of Zygomorphic Corolla Development In Bee And Hummingbird Pollinated Species Of Schizanthus (Solanaceae), Ghadeer Bukhari

Theses and Dissertations

This Ph.D. dissertation investigates two main projects separated into two chapters, one for each. The first chapter explores the evolution of flowering plants (angiosperms), a macro-scale study. The second project is on a micro-scale that focus on exploring the genes that regulate the similarities and the differences of flower morphology reflecting their different pollination syndromes in two species of Schizanthus (Solanaceae), i.e., S. pinnatus, a bee-pollinated flower, and S. grahamii, a hummingbird- pollinated flower.

In the first chapter, I used floral diagrams to study the origin and the evolution of floral traits in angiosperms, a new set of morphological data …

A Discrete Age Structured Model Of Hantavirus In A Rodent Reservoir In Paraguay, Megan A. Rúa

Biological Sciences Faculty Publications

Many rodent-borne hantaviruses are zoonotic pathogens that can cause disease in humans through inhalation of aerosolized rodent excreta. To evaluate the prevalence of Jaborâ virus (JABV) over time within its rodent reservoir, Akodon montensis, we formulated a mathematical model with multiple rodent age classes and unique infection class progression features. We then parameterized the model with data collected from a survey of JABV harbored by Akodon montensis in the Mbaracayú Reserve in Paraguay. Our model incorporates three types of infection over the lifetime of the rodent as well as a recovered class. A new feature of the model allows transition …

Food Web Effects Of Lake Trout (Salvelinus Namaycush) Invasion In Northwestern Montana, Charles Wainright

Graduate Student Theses, Dissertations, & Professional Papers

Around the turn of the 20th century, lake trout (Salvelinus namaycush) were widely introduced in several lakes and reservoirs outside their native range in western North America. Since then, lake trout have become problematic in many lakes where they were introduced, causing significant declines in popular sport fishes and native species, most notably federally protected bull trout (Salvelinus confluentus). Despite evidence that invasive fish can cause cascading trophic effects in aquatic communities, the impacts of lake trout introduction / invasion on aquatic food webs remain poorly understood. Moreover, native fish restoration programs tend to focus on suppression …

Characterizing The Requirements For The Matricellular Protein, Dccn, In Nervous System Function, Elizabeth L. Catudio Garrett

Graduate Student Theses, Dissertations, & Professional Papers

The brain is organized as a complex network of specialized neurons that communicate via a combination of electrical and chemical signals. Our brains function to generate movement, control organ function, or direct complex behaviors; all of which requires the ability to regulate the flow of communication between circuits and networks. Work in this thesis addresses two areas of neuron communication: first, how does the release of more than one neurotransmitter from a single neuron impact behavior, and second, are matricellular proteins (MCPs) key contributors to synaptic transmission and neuron function? The conserved CCN family of MCPs have a …

Non-Invasive Method For Leptin Supplementation In Zebrafish (Danio Rerio), Regan Mcnamara

Williams Honors College, Honors Research Projects

I tested the hypothesis that recombinant leptin protein can be introduced to zebrafish in vivo through non-invasive soaking in a solution containing the protein. One way to study various molecules’ effects in vivo is through intraperitoneal or intracerebroventricular injections during the embryonic or larval stage, which is invasive, difficult to administer, and can have a high mortality rate. 48 hours post fertilization (hpf) zebrafish were soaked in a His-tagged recombinant leptin protein solution at 10 nM and 100 nM concentrations (produced by Genscript). After soaking, zebrafish larvae were washed extensively to remove all recombinant protein on their exterior before homogenization. …

Small Mammal Communities In Grasslands At The Grand Forks Air Force Base, North Dakota, Usa, Lynda R. Lafond, Elizabeth H. Rave, Kathryn A. Yurkonis

The Prairie Naturalist

Small mammals are important in grasslands but are often overlooked in management and reconstruction efforts. We sampled small mammals in three sites on the Grand Forks Air Force Base (GFAFB) located in central Grand Forks County, North Dakota, USA. The study sites varied in their management history and represented the three major types of grasslands (reconstructed prairie, old field, and hay field) within Grand Forks County. We captured 463 individuals of six species with Sherman live traps in summer (June, July, August) 2014 and 2015. We captured the most individuals and species (295 individuals of 5 species), including all shrews …

Captive Ring-Necked Pheasant Response To Very High Experimental Doses Of Lead, Travis J. Runia, Alex J. Solem

The Prairie Naturalist

Ingestion of spent lead pellets is a well-documented source of lead exposure in free-ranging birds, although the consequence of lead ingestion varies widely among avian guilds. Ring-necked pheasants (Phasianus colchicus) appear to be less susceptible to lead poisoning than other game birds. Our objectives were to determine survival, liver lead accumulation, and body mass change of 129 captive-raised pheasants in response to being gavage-fed 5, 10, 20, or 40 lead pellets. All pheasants survived the 21-day experiment. Liver-lead levels were positively correlated with the number of lead pellets retained and negatively correlated with beginning body mass. Change in percent body …

The Host Gatekeeper: Using The Flagellar Pathway To Understand Symbiont Host Adaptation, Adam R. Pollio

Graduate Theses, Dissertations, and Problem Reports (ETD)

The acquisition of microbial partners is a strategy used by a diverse group of arthropods to overcome ecological barriers that might normally make certain niches uninhabitable. The unique phylogenetic opportunities attainable from the natural experiment of the Sodalis-allied clade allow for better understanding of how molecular structures evolve through time. Here, we focus on the evolution of the flagellar synthesis pathway, due to its complexity and ability to diverge in response to ecological pressures. We used this molecular pathway and natural experiment to show that normal evolutionary outcomes associated with symbiosis (i.e., genome reduction) do not explain the predicted conservation …

The Exploration Of Nanotoxicological Copper And Interspecific Saccharomyces Hybrids, Matthew Joseph Winans Phd

Graduate Theses, Dissertations, and Problem Reports (ETD)

Nanotechnology takes advantage of cellular biology’s natural nanoscale operations by interacting with biomolecules differently than soluble or bulk materials, often altering normal cellular processes such as metabolism or growth. To gain a better understanding of how copper nanoparticles hybridized on cellulose fibers called carboxymethyl cellulose (CMC) affected growth of Saccharomyces cerevisiae, the mechanisms of toxicity were explored. Multiple methodologies covering genetics, proteomics, metallomics, and metabolomics were used during this investigation. The work that lead to this dissertation discovered that these cellulosic copper nanoparticles had a unique toxicity compared to copper. Further investigation suggested a possible ionic or molecular mimicry …

Combinatorial Discovery And Validation Of Heptapeptides With Utp Binding Induced Structure, Daniela Kroiss, James Aramini, Sangitaa Ragoonath, Rein V. Ulijn, Tell Tuttle

Advanced Science Research Center

In biology, supramolecular recognition typically involves an ‘induced-fit’ mechanism, where structures rearrange upon complexation to accommodate binding ligands. Designing minimalistic compounds with such adaptability is challenging as they involve subtle conformational changes that are energetically similar. Here, we demonstrate the integration of combinatorial screening with molecular modelling to identify heptapeptides that form a stable loop upon recognition of uridine triphosphate (UTP). Peptide sequences selected using phage display were refined computationally and correlated with experimental KD values. This combined approach may serve as a method for the de novo selection and subsequent rationalization of the compositional and organizational principles that dictate …

Pharmacological Or Genetic Targeting Of Transient Receptor Potential (Trp) Channels Can Disrupt The Planarian Escape Response, Ziad Sabry , '21, A. Ho, Danielle Ireland, Christina Rabeler, O. Cochet-Escartin, Eva-Maria S. Collins

Biology Faculty Works

In response to noxious stimuli, planarians cease their typical ciliary gliding and exhibit an oscillatory type of locomotion called scrunching. We have previously characterized the biomechanics of scrunching and shown that it is induced by specific stimuli, such as amputation, noxious heat, and extreme pH. Because these specific inducers are known to activate Transient Receptor Potential (TRP) channels in other systems, we hypothesized that TRP channels control scrunching. We found that chemicals known to activate TRPA1 (allyl isothiocyanate (AITC) and hydrogen peroxide) and TRPV (capsaicin and anandamide) in other systems induce scrunching in the planarian species Dugesia japonica and, except …

Recent Observations Of Water Shrews In Northeastern South Dakota, Dennis Skadsen, Robert M. Timm

The Prairie Naturalist

North American water shrews in the genus Sorex are a complex of at least five species, three of which were recognized historically, Sorex alasksans, S. bendirii, and S. palustris (Hall 1981). Within what was previously considered the single, widespread northern species, S. palustris, two additional species are now recognized, S. albibarbis in the eastern US and Canada and S. navigator in the western United States and Canada (Hope et al. 2014; Nagorsen et al. 2017; Woodman 2018). The American water shrew (Sorex palustris) originally was documented in South Dakota by three females, two were collected 1876 and …

Review Of Sky Dance Of The Woodcock: The Habits And Habitats Of A Strange Little Bird, By Greg Hoch, David E. Andersen

The Prairie Naturalist

American Woodcock (Scolopax minor) have enthralled conservationists (including Aldo Leopold), bird watchers, wildlife enthusiasts, hunters, and others interested in the natural world for centuries. No doubt, woodcock also have enthralled humans in North America for millennia prior to written descriptions of the woodcock’s courtship displays, habitat preferences, and curious behavior and anatomy. As perhaps the most extensively studied species of shorebird in the world, there is a rich and extensive literature, both scientific and popular, focused on woodcock ecology, behavior, and hunting. To that extensive body of literature, Sky Dance of the Woodcock provides an updated summary of …

The Prairie Naturalist, Vol. 51, Issue 2, December 2019

The Prairie Naturalist

45 EDITOR’S NOTE: Final Thoughts as Editor-in-Chief, by Christopher N. Jacques

RESEARCH ARTICLES

47 Bats of the Loess Hills Ecoregion of Southeast Nebraska, by Virgil Brack, Jr., Dale W. Sparks, and Darwin C. Brack

58 Serological Survey and Pathogen Exposure of Adult Female White-tailed Deer in the Western Dakotas, by Katherine L. Moratz, Bailey S. Gullikson, Eric S. Michel, Daniel M. Grove, Jonathan A. Jenks, and William F. Jensen

68 Factors Limting Reintroduced Plains Topminnow, Fundulus sciadicus, Populations in Central Great Plains Streams, by Joseph D. Thiessen, Keith D. Koupal, and Casey W. Schoenebeck

NOTES

77 Recent Observations of Water …

Factors Limiting Reintroduced Plains Topminnow, Fundulus Sciadicus, Populations In Central Great Plains Streams, Joseph Thiessen, Keith D. Koupal, Casey W. Schoenebeck

The Prairie Naturalist

The plains topminnow (Fundulus sciadicus) is an endemic Great Plains stream fish that has experienced declines in geographic range and local abundance. Due to these declines, the species has been considered for federal protection and designated with conservation status in states throughout its historic range. The reasons for declines are likely similar to hypothesized factors for other endemic stream fish declines in the Great Plains. To investigate potential limiting factors a suite of 17 historic sites with reintroduced plains topminnow populations across Nebraska were evaluated for current populations and if plains topminnow were absent, additional fish were introduced. …

The Mayfly Newsletter, Donna Giberson

The Mayfly Newsletter

The Mayfly Newsletter is the official newsletter of the Permanent Committee of the International Conferences on Ephemeroptera

Final Thoughts As Editor-In-Chief, Christopher N. Jacques

The Prairie Naturalist

Greetings GPNSS members! I write this editorial during a time of reflection as Editor-in-Chief of The Prairie Naturalist (TPN), and during unprecedented times as the global COVID-19 pandemic continues. In full disclosure, I do not have a particular topic for this editorial, other than to offer a few final thoughts as my time serving the Great Plains Natural Science Society and TPN.