Marine Biology Commons^™

Fish Assemblage Dynamics And Red Drum Habitat Selection In Bayou St. John And Associated Urban Waterways Located Within The City Of New Orleans, Louisiana, Patrick W. Smith Mr.

LSU New Orleans Theses and Dissertations

Bayou St. John (BSJ) and City Park Lakes and Lagoons (CPLL) are urban waterways in New Orleans, Louisiana. I studied habitat selection of red drum in BSJ, and fish assemblage change in BSJ and CPLL over 40 years. Temperature was found to be the best predictor of red drum habitat selection in Bayou St. John, while salinity and change in depth also were found to be good predictors for certain sites. Potential prey item abundance did not appear to influence habitat selection. Using data from 1971 – 2010, nearshore habitats in CPLL were affected by Hurricane Katrina, but have sense …

Nitrogen, Phosphorus, And Suspended Solids Concentrations In Tributaries To The Great Bay Estuary Watershed In 2011, New Hampshire Department Of Environmental Services, Matthew A. Wood, Philip Trowbridge

PREP Reports & Publications

Nitrogen, phosphorus, and sediment loads to the Great Bay Estuary are a growing concern. The Piscataqua Region Estuaries Partnership (PREP) calculates the nitrogen load from tributaries to the Great Bay Estuary for its State of the Estuaries reports. Therefore, the purpose of this study was to collect representative data on nitrogen, phosphorus, and suspended sediment concentrations in tributaries to the Great Bay Estuary in 2011. The study design followed the tributary sampling design which was implemented by the New Hampshire Department of Environmental Services between 2001 and 2007 and by the University of New Hampshire in 2008 and 2010, so …

Salinity Preference Of Alaskan Threespine Stickleback: Test For Divergence In Halotaxis Between Ancestral And Landlocked Populations, David C. Fryxell

Honors Scholar Theses

Glacial retreat during the Pleistocene caused landlocking of anadromous Alaskan threespine stickleback, Gasterosteus aculeatus, furnishing a natural 'experiment' in osmoregulatory divergence. The objective of this study was to assess the effects of individual acclimation and population divergence on salinity preference. Full-sibling families of marine, anadromous, and freshwater-landlocked populations of stickleback were reared in common environments until 3 weeks post-hatch, then were split and acclimated to low or high salinity. At 6 to 8 weeks of age the six experimental groups were tested for salinity preference in a tank that offers fish a choice of compartments with different salinities arranged in …

Effectiveness Of Marine Protected Areas Across A Latitudinal Gradient, Chelsea Roy

Honors Scholar Theses

Use of marine protected areas (MPA) as a tool for conservation and sustainable use of marine biological diversity is increasing worldwide. However, the geographic extent of MPA designation varies, as does a full understanding of the ecological utility of such designations. Progress towards marine conservation goals needs to be evaluated in order to determine areas lacking effective MPA designation. The goal of this study is to evaluate the representation (in terms of communities and habitats) and performance of marine protected areas in the Northwestern Atlantic and Northeastern Pacific across a latitudinal gradient. Presence or absence of MPAs in specific ecological …

An Approach For Use Of Dual Frequency Identification Sonar (Didson) To Quantify Behavioral Aspects Of Piscivory At Ecologically Relevant Time And Space Scales, Victoria E. Price

Master's Theses

Predator-prey interactions of large vagile fishes are difficult to study in the ocean due to limitations in the space and time requirements for observations. Small-scale direct underwater observations by divers (<10m >radius) and large-scale hydroacoustic surveys (10s - 100s km2) are traditional approaches. However, large piscivorous predators identify and attack prey at the scale of meters to tens of meters. Dual- Frequency Identification Sonar, or DIDSON, is a high-resolution acoustic camera operating in the MHz range that provides detailed continuous video-like imaging of objects out to 30 m range. This technology can be used to observe predator-prey interactions at ecologically …

Big Blue And You: An Interdisciplinary Look At Science And The Ocean, A Students Teaching Students Course, Megan M. Nepshinsky, Alexa Kretsch, Benjamin Negrete

Senior Honors Projects

This class is the second project under the new Honors Program initiative, Students Teaching Students (STS). STS was brought to URI and implemented by Bridget Griffith as part of her Senior Honors Project in 2011. Students apply in their Junior year to design, research, and teach an honors course on a topic about which they are passionate. The STS program allows motivated honors students to have a unique opportunity to lead a class as student professors. This allows students a freedom of creativity on the topic, assignments, and teaching style. Drawing on our own experiences, we were able to create …

Big Blue And You: An Interdisciplinary Look At Science And The Ocean, A Students Teaching Students Course, Benjamin Negrete Jr., Megan Nepshinsky, Alexa Kretsch

Senior Honors Projects

This class is the second project under the new Honors Program initiative, Students Teaching Students (STS). STS was brought to URI and implemented by Bridget Griffith as part of her Senior Honors Project in 2011. Students apply in their Junior year to design, research, and teach an honors course on a topic about which they are passionate. The STS program allows motivated honors students to have a unique opportunity to lead a class as student professors. This allows students a freedom of creativity on the topic, assignments, and teaching style. Drawing on our own experiences, we were able to create …

Genetic Variation In Potentially Virulent Vibrio Parahaemolyticus From The Northern Gulf Of Mexico, Nicholas Felix Noriea Iii

Dissertations

Vibrio parahaemolyticus (V_p) is a gram-negative bacterium found naturally in marine and estuarine environments. V_p is found in oysters including those which are later consumed by the public. Sub-populations of potentially virulent Vp contain specific virulence factors and are relevant human pathogens capable of causing gastroenteritis, wound infection, and death. The tdh and trh genes, both encoding hemolysins, have been correlated with the majority of clinical V_p isolates but have not been shown to be the definitive virulence factors.

A total of 146 V_p isolates from the northern Gulf of Mexico were collected and probed …

Effects Of Hypoxia And 4-Tert-Octylphenol On Gene Expression Profiles Of The Sheepshead Minnow (Cyprinodon Variegatus), Arthur Alan Karels

Dissertations

Hypoxia occurs in estuaries of northern Gulf of Mexico and world-wide, with increasing frequency/severity via eutrophication and anthropogenic influences. Hypoxia inducible factors (HIFs) form transcriptional complex and bind DNA at hypoxia responsive elements (HREs) in promoter regions of genes needed for systemic and cellular adaptation of fish to low dissolved oxygen (hypoxia, DO <2.0 mg/ml). Hypoxia-induced activation of HIF-αs can lead to a cascade of downstream activation, such as erythropoietin (EPO). Return to normal DO levels (normoxia), prolyl hydroxylases (PHDs) are activated to degrade HIF-αs back to baseline. Fish are affected by environmental estrogen mimics, like 4-tert-octylphenol (4tOP), binding estrogen receptor alpha (ERα) at estrogen responsive elements (EREs) and activating genes vitellogenin (VTG). Previous research showed overlap or crosstalk between these two mechanistic pathways. Hypoxia triggers unknown factors regulating ERE-mediated ERα signaling pathway, and stressor combinations could increase/decrease hypoxic or endocrine pathway. Research examined molecular/physiological effects of hypoxia (acute and chronic, moderate and severe) and 4tOP (~60μg/L)on adult male and/or female sheepshead minnow (Cyprinodon variegatus). Three genes identified, cloned, and sequenced (HIF-1α, HIF-2α, and PHD3), plus previously identified genes EPO and VTG, were examined in liver/testes exposed to hypoxia and/or 4tOP for cellular/physiological changes. Endpoints examined included mRNA expression from real-time PCR of HIF-1α, HIF-2α, PHD3, EPO, and VTG using cDNA from total RNA extracts, and microarray analyses of genes expressed during the transition from hypoxia back to normoxia. Phylogenetic analyses confirmed isolation of two HIF-α isofoms (HIF-1α and HIF-2α) and the PHD3 isoform. Significant up-regulation of PHD3 occurred within 10 hrs of chronic hypoxia, and persisted when severe (1.5 mg/L) and declined when moderate (~2.5mg/L). Significant up-regulation of HIF-1α and EPO occurred within 30 minutes to 2 hours of onset of acute severe and very severe (~1.08mg/L) hypoxia. Hypoxia acted similar to an estrogen mimic, with huge up-regulation of VTG gene expression in males, and increased VTG levels (additive effect) when hypoxia was combined with 4tOP. Microarray analyses showed 125 genes with significant transcriptional change, with up- or down-regulation from transitions of: (1) hypoxia (72 hrs) to normoxia (74 hrs) and (2) hypoxia+4tOP (72 hrs) to normoxia+4tOP (74 hrs).

Comparison Of Creel Survey Data To Traditional Sampling Techniques In Pit-Lake Fisheries Of Muhlenberg County, Kentucky, Derek L. Rupert

Masters Theses & Specialist Projects

Populations of largemouth bass, Micropterus salmoides, and bluegill, Lepomis macrochirus, were evaluated from five pit-lakes in Muhlenberg County, Kentucky, to determine if accurate proportional stock density (PSD) data can be obtained from a mandatory creel survey. It was hypothesized that the proportion of stock-to-quality (300-400mm) and quality (+400mm) largemouth bass from four years (2007-2010) of creel survey data would be statistically similar to those generated through on-site sampling in 2011. Fish were collected via a combination of gill netting, seining, hook-and-line fishing, and boat-mounted electro-fishing. In two of the pit-lakes, the sampling-generated length frequency data was not significantly …

Predictive Model Of Ocean Sediment Binding, Erica Delaney

Honors Senior Capstones and Theses

This project explored the relationship between sediment size, sediment composition and the concentration of mercury. The samples were taken from Narragansett Bay in Rhode Island. Knowledge and understanding of the binding ability of heavy metals, specifically mercury, will help to improve remediation efforts in the future. Analysis of elemental composition was conducted using the Elementar Analyzer and much troubleshooting of the instrument was necessary. End result of the project was the ability to work successfully with an advisor, as well as the ability to troubleshoot, operate and maintain the instrument.

Red Sea Coral Reef Trajectories Over 2 Decades Suggest Increasing Community Homogenization And Decline In Coral Size, Bernhard Riegl, Andrew W. Bruckner, Gwilym Rowlands, Samuel J. Purkis, Philip Renaud

Marine & Environmental Sciences Faculty Articles

Three independent line intercept transect surveys on northern Red Sea reef slopes conducted in 1988/9 and 1997/8 in Egypt and from 2006–9 in Saudi Arabia were used to compare community patterns and coral size. Coral communities showed scale-dependent variability, highest at fine spatial and taxonomic scale (species-specific within and among reef patterns). At coarser scale (generic pattern across regions), patterns were more uniform (regionally consistent generic dominance on differently exposed reef slopes and at different depths). Neither fine- nor coarse-scale patterns aligned along the sampled 1700 km latitudinal gradient. Thus, a latitudinal gradient that had been described earlier from comparable …

Use Of Injectable Eugenol For Euthanasia And Anesthesia Of American Lobsters (Homarus Americanus) And Similar Species, Carlton Huntsberger

Biology Theses

Crustaceans are economically and ecologically significant, but current treatment and diagnostic protocols for crustacean health are limited. According to standards given by the American Veterinary Medical Association there are no current methods of euthanizing lobsters, nor are there effective methods of quick release anesthesia. The objective of this research demonstrated that eugenol by direct injection is a safe, efficient and reliable method for euthanizing or anesthetizing crustaceans. Anesthetic levels were determined by behavior responses, death was determined by a lack of response to stimuli. The results presented here suggest eugenol can be used as a euthanizing agent for American lobsters …

Evaluation Of Electrophoretic Protein Extraction And Database-Driven Protein Identification From Marine Sediments, Eli K. Moore, Brook L. Nunn, Jessica F. Faux, David R. Goodlett, H. Rodger Harvey

OES Faculty Publications

Intact proteins comprise a major component of organic carbon and nitrogen produced globally and are likely an important fraction of organic matter in sediments and soils. Extracting the protein component from sediments and soils for mass spectral characterization and identification represents a substantial challenge given the range of products and functionalities present in the complex matrix. Multiple forms of gel electrophoresis were evaluated as a means of enhancing recovery of sedimentary protein before proteomic characterization and compared with a direct enzymatic digestion of proteins in sediments. Resulting tryptic peptides were analyzed using shotgun proteomics and tandem mass spectra were evaluated …

Investigating The Roles Of Lhx1a, Lhx1b, And Lhx5 In Zebrafish Spinal Cord Interneuron Specification And Function, Samantha Balakirsky

Renée Crown University Honors Thesis Projects - All

Located in the vertebral column, the human spine is responsible for regulating body movements and receiving sensory input about pain and touch. Currently, few treatments for neurological diseases and spinal cord injuries exist, partly because we know little about how a fully functioning spinal cord is constructed. As such, studying spinal cord development, specifically neuronal specification and patterning, should be useful for developing better treatments for people with spinal cord injuries and diseases. Zebrafish are a prime model organism for studying neuronal specification because their transparent embryos develop outside the mother, allowing us to easily examine gene expression, cell movements …

Application Of A Hybrid 3d-Var Data Assimilation System In The Monterey Bay To Study Regional Dynamics Of The California Current System, Chudong Pan

Dissertations

A data assimilation system combining 3-dimensional variational scheme and Navy Coastal Ocean Model was applied to the Monterey Bay area to assimilate temperature and salinity glider data collected in August 2003. The hybrid background error covariance model in the present 3-dimensional variational system incorporates both the static and the flow-dependent background error covariance. To explore the impact of high temporal resolution on the overall skill of the assimilation system, the intended data assimilation interval was set to 1 hour in the present study. A Floating Temporal Window approach is designed to keep the computational efficiency of the scheme and to …

Potential Impact Of Herbivorous Fish On Ulva Bloom Biomass, Tanja Schollmeier

Senior Honors Projects

Potential Impact of Herbivorous Fish on Ulva Bloom Biomass

Tanja Schollmeier

Sponsor: Carol Thornber, Biological Sciences

The addition of anthropogenically produced nitrogen and phosphorous to coastal habitats is a major problem that frequently results in the rapid growth, or bloom, of macroalgae. Within Narragansett Bay, RI, blooms are commonly formed by two species: Ulva compressa and U. rigida. These blooms co-occur with a number of invertebrates that have been previously studied for their impacts on bloom biomass. However, few studies have examined the abundance or potential grazing impacts of herbivorous fish. To address this information gap, we conducted surveys …

Double-Power Double-Heterostructure Light-Emitting Diodes In Microalgae, Spirulina Platensis And Nannochloropsis Oculata, Cultures, Yean-Chang Chen, Meng-Chou Lee

Journal of Marine Science and Technology–Taiwan

The growth conditions of two microalgae, Spirulina platensis and Nannochloropsis oculata, were tested under various light sources including white cold DDH GaAlAs LED, traditional fluorescent lamps and natural light cultures, with no difference found in the growth of biomass, indicating that white cold DDH GaAlAs LED could replace traditional fluorescent lamps, thus providing for large saving on electricity costs and preservation of cultural space. To date, the use of white cold DDH GaAlAs LEDs as light source for microalgal cultures seems to be largely unstudied.

Thermal Impacts Of A Coal Power Plant On The Plankton In An Open Coastal Water Environment, Keun-Hyung Choi, Young-Ok Kim, Joon-Baek Lee, Soon-Young Wang, Man-Woo Lee, Pyung-Gang Lee, Dong-Sik Ahn, Jae-Sang Hong, Ho-Young Soh

Journal of Marine Science and Technology–Taiwan

Over a five-year period, this study examined the overall impact of coal power plant cooling processes on entrained copepods and the local plankton community on the west coast of Korea. Mortality differences between the intake and discharge water of the single most dominant copepod, Acartia hongi, were positively correlated with temperature differences between the two locations. Laboratory tests showed copepod sensitivity to temperature increase, and with very low chlorine concentration applied, thermal stress was the major source of copepod mortality. Chlorophyll a concentration, ciliate abundance, and total copepod abundance at the intake showed no discernable differences from the values at …

Eelgrass Habitat Creation In Nantucket Harbor, Massachusetts, David M. Burdick, Gregg Moore, Chris Peter

Natural Resources & the Environment

In response to eelgrass habitat losses associated with development and marine activities in and around Nantucket Harbor, a plan to restore a meadow by transplanting eelgrass to previously vegetated areas was developed in conjunction with the Nantucket Land Council. Over 6,000 eelgrass shoots were sustainably harvested from an extensive bed within the Harbor that was located just west of First Point and near the inlet to Nantucket Sound. Four weeks following collection, impacts from our collection were shown by a 24% decline in shoot density, but live eelgrass cover did not decline significantly. After 12 weeks, no effects of collecting …