Laboratory and Basic Science Research Commons^™

The Effect Of Barometric Pressure On Feeding Activity Of Yellow Perch, Daniel Vanderweyst

Journal of Earth and Life Science

This is the first study to our knowledge that has attempted to relate feeding activity of fish to barometric pressure. This study was conducted with the expectation that a difference in food ingestion would occur in a game fish species at different barometric pressures. The overall objective of this study is to determine if barometric pressure influences the feeding activity of a popular game fish. Yellow perch (Perca flavescens) were collected by seine and trap net from Lake Bemidji 12 September through 24 October 2013. The range in size and weight of yellow perch used for this study …

Use Of Cast Nets And Seine Hauls To Estimate Abundance Of Age-0 Yellow Perch, Michael Vaske

Journal of Earth and Life Science

Knowledge about fish population dynamics is essential for developing management plans and evaluating management success. In many lakes, yellow perch (Perca flavescens) are the main forage for many upper level predators and recreational fish species. To better manage recreational fisheries, an accurate estimate of prey abundance is necessary. The objectives of the study were to sample the littoral and limnetic zone to better estimate population of age-0 yellow perch, and obtain an estimate of the proportion of yellow perch being missed by traditional littoral seining methods. Littoral seines were conducted at three randomly selected locations in the south …

Changing Body Condition In Walleye With Reference To Ideal Sample Size Requirements, Kevin Longtin

Journal of Earth and Life Science

The objective of this study was to determine if walleye Sander vitreus percent dry weight, a measure of condition, differed among summer months of 2014 on Maple Lake, Minnesota. A second objective was to determine if there was a relationship between condition and body temperature, total length, or wet weight. Between three and six walleyes were collected per month by hook and line methods. All fish had internal body temperatures taken at the time of capture and were oven dried in the laboratory at 80 °C in order to measure percent dry weight. There was no relationship between condition and …

Receptor Of Advanced Glycation Endproducts (Rage) Is Positively Correlated With Tumor Necrosis Factor-Α In Adolescents With Obesity, Tasnim Rahman, Daniel H. Conrad, Anshu Gupta

UROP Posters

Introduction: Obesity in childhood is associated with an increased prevalence of diabetes and other traditional cardiometabolic risk factors, suggesting an epidemic of premature cardiovascular disease among today’s youth. Glycotoxins, known as advanced glycationend products (AGE’s), activating via the membrane-bound receptors (mRAGE), have been implicated in the pathophysiology of inflammation, (increased tumor necrosis factor-α [TNF-α ]), insulin resistance and vascular dysfunction in adults, but the role of RAGE in the early stages of metabolic disorders is unknown. In this study, we assessed relationship of cardiometabolicrisk factors, mRNA expression of TNF-α and RAGE in peripheral monocytes in adolescents with obesity.

Methods: Thirty …

The Effects That Liquid And Solid Cattle Manure Have On The Water Quality Of Drainage Ditches In Putnam County, Ohio, Janelle Horstman

Honors Projects

Lake Erie has experienced harmful algal blooms with increased frequency since the mid-1990s due to excess nutrients from Rivers, such as the Maumee River, and largely agricultural watersheds. Nonpoint source pollution from agriculture contributes to eutrophication, algal blooms, and the degradation of water quality. This creates stress on aquatic fauna, reduced aesthetic quality, odor, and limits of the water for usage of drinking, recreation, and industry. This research paper asks what the contributions of having access to manure application records, soil records, and information about antibiotics have on what is known about manure management and antibiotic resistance, which has been …

Defining The Physiochemical, Biological And Habitat Requirements For The Upper Extant Of Oncorhynchus Mykiss Distribution, Emily Powers

Journal of Earth and Life Science

The distribution of rainbow trout is strongly influenced by their habitat. As climatic conditions change, the distribution of rainbow trout populations will change along with them. The objective of this study was to identify the particular habitat parameters that rainbow trout select for in this lotic system and to specifically assess how this relates to the upper extant of their distribution. By assessing the distribution of the upper extant specifically, future research may be conducted to better assess climatic changes on a small and/or large scale basis. A 9,000 m section of the Clearwater River was used as the study …

The Effects Of Angling Pressure On Northern Pike Size Structure, Ryan Carrow

Journal of Earth and Life Science

A number of conditions within a lake system can affect northern pike (Esox lucius) size structure. Of these conditions, the amount of angling pressure may have the biggest effect on the size structure of northern pike in Minnesota lake systems. In this study, five private and five public lakes were chosen near Bemidji, Minnesota. Each lake was fished for twenty angling hours using live northern hog suckers (Hypentelium nigricans) or golden shiners (Notemigonus crysoleucas). Total length (mm) and weight (kg) were measured for each northern pike caught. The number of fish houses that were …

The Impact Of Dams On Nitrogen Cycling In The Messalonskee Stream, Rebecca K. Forgrave

Honors Theses

The Messalonskee Stream in central Maine has five hydroelectric dams on 16.6km. Each dam drastically changes the flow regime of the stream, dividing it into segments with different patterns of sediment settling and organic matter retention. I investigated how these disruptions impact nitrogen cycling, specifically nitrification rates above and below each dam. I expected higher nitrification rates above the dams, where levels of organic matter are higher, and lower rates below the dam where scouring removes organic matter and fine sediment from the streambed. I measured sediment nitrification rates with a nitrapyrin-inhibition assay and potential drivers of nitrification including sediment …

Pcb Effects On Development Of Behavior In Rats, Robert Witte

Honors Projects

Abstract

PCBs were originally used as industrial chemicals in many devices and applications, including electrical capacitors and transformers, lubricants, and sealants in buildings. PCBs have been investigated and their use has been slowly phased out starting in the U.S. in the 1970s. A worldwide ban was put on the use and manufacturing of PCB in 2001, however they remain in the food chain because of their lipophilic properties and their long half-life. Major sources of PCB include wild-life, fish, and other seafood. Individuals exposed to PCB during development can experience negative effects on neurobiological, cognitive, and behavior functioning in both …

A Brief Elevation Of Serum Amyloid A Is Sufficient To Increase Atherosclerosis, Joel C. Thompson

Theses and Dissertations--Pharmacology and Nutritional Sciences

Cardiovascular disease is now the leading cause of death worldwide. Serum amyloid A (SAA), a positive acute phase reactant, along with C-reactive protein is used clinically as a marker of cardiovascular disease risk. However, recent data has shed light on a possible causal role of SAA in the development of atherosclerosis, the most pervasive form of cardiovascular disease. Several inflammatory diseases such as diabetes and obesity are known to confer increased risk of developing cardiovascular disease. Individuals with these diseases all have modest but persistent elevation of SAA. To determine if SAA caused the development of atherosclerosis, apoe^-/-chow …

Winter Survival Strategies Of Benthic Invertebrates In Temporary Habitats In A Northern Minnesota Lake, Cal Oldenburg

Journal of Earth and Life Science

During the winter months in the northern hemisphere, above 40 degrees latitude, a freezing event occurs that can last several months. Some lakes may have large portions of the shallow littoral zone frozen completely solid down into the sediments. Invertebrates affected by these events have developed survival strategies to cope with the harsh environment. The two main strategies for invertebrates in these areas are to either migrate to a different area and recolonize the original area when it becomes available again, or to burrow into the sediments and withstand the freezing. A 1- month study took place on Lake Bemidji …

Report And Recommendations Of The Balance Of Crop Research Working Group Of The National Agricultural Research, Extension, Education And Economics (Nareee) Advisory Board, Mark Mclellan, Leo Holt, Twilya L'Ecuyer, Robert Taylor, Julia Sabin, Milo Shult, Anne Marie Thro, Paul Heisey

Biology Faculty Publications and Presentations

In December of 2012, the President’s Council of Advisors on Science & Technology (PCAST) issued a report to the President on Agricultural Preparedness and the Agriculture Research Enterprise. The PCAST report recognizes the long-term history of public investment in agricultural research. The report also recognized significant private investment into agricultural research. Significantly, the report is adamant that the agricultural research enterprise is not prepared to meet the challenges of the 21st century. The report cited two reasons:

1. too little competitive research and
2. an imbalance of research priorities between private and public funding sources.

This report addresses the latter issue.

Members …

Targeted Knockout Of Beclin-1 Reveals An Essential Function In Ovary And Testis, Thomas R. Gawriluk

Theses and Dissertations--Biology

An estimated 12% of couples worldwide are infertile. The contributing factor is approximately equal between men and women with nearly 25% diagnosed as idiopathic. Despite the increasing numbers of couples seeking assistance from infertility clinics, few molecular mechanisms have been identified for treatment. Autophagy is an evolutionarily conserved cellular process for bulk degradation and recycling of cytosolic components through the lysosome to maintain homeostasis. Several studies have observed increased levels of autophagy during ovarian folliculogenesis and gonadal steroidogenesis; however, no genetic studies to determine the significance of autophagy exist.

To investigate the function of autophagy in the ovary and testis, …

The Prevalence And Effect Of Wolbachia Infection On The Brown Widow Spider (Latrodectus Geometricus), Brittany Dane' Arrington

College of Graduate Studies: Theses & Dissertations

Wolbachia was recently identified in the Brown Widow Spider (Latrodectus geometricus). This symbiont has not previously been found in this genus, therefore the consequences on the host’s population dynamics are unknown. The frequency of endosymbiont infection can provide insight into the population dynamics of the host as this bacteria is known to alter host life history. In my 1^st chapter I determine the prevalence of Wolbachia pipentis infection in in the Brown Widow Spider (Latrodectus geometricus) from five locations in the Southeastern United States. Infection frequency differed significantly among sampling locations, ranging from 20% to …

Observing The Unwatchable Through Acceleration Logging Of Animal Behavior, Danielle D. Brown, Roland Kays, Martin Wikelski, Rory Wilson, A. Peter Klimley

Methodology and Animal Models in Research

Behavior is an important mechanism of evolution and it is paid for through energy expenditure. Nevertheless, field biologists can rarely observe animals for more than a fraction of their daily activities and attempts to quantify behavior for modeling ecological processes often exclude cryptic yet important behavioral events. Over the past few years, an explosion of research on remote monitoring of animal behavior using acceleration sensors has smashed the decades-old limits of observational studies. Animal-attached accelerometers measure the change in velocity of the body over time and can quantify fine-scale movements and body postures unlimited by visibility, observer bias, or the …

Investigation Of The Role Of Tor And Nutrient Restriction On Lifespan Of Arabidopsis Thaliana, Debashree Banerjee

Dissertations, Masters Theses, Capstones, and Culminating Projects

The Target of Rapamycin (TOR) kinase is evolutionarily conserved and is a key regulator, controlling many cellular functions to promote survival and growth in all eukaryotes. Several model organisms have indicated a relationship between TOR signaling and life expectancy, such as yeast, fruit flies and mice. Abundant nutrient availability promotes rapid growth and development, whereas depletion of nutrients reduces the activity of the pathways that are involved in growth and nutrient processing. This reduction in activity increases life expectancy, which is supported by evolutionary theories. The confirmed presence of the TOR homolog (a major regulator of growth and cell proliferation) …

Developmental And Molecular Functions Of Plakophilin-3, William A. Munoz

Dissertations and Theses (Open Access)

Plakophilin-3, the less studied member of the plakophilin-catenin subfamily, and the larger catenin family, binds directly to desmosomal cadherin cytoplasmic domains and enhances desmosome formation and stability. In mammals, plakophilin-3 is expressed at the highest levels in desmosome-enriched tissues such as epithelia, with the knock-out in mice producing corresponding reductions in ectodermal integrity. In tissue, cellular and intracellular contexts where plakophilin-3 is not at the desmosomal plaque, little is known about its functions in the cytoplasm or nucleus, where it also localizes.

My work employed embryos of the amphibian, Xenopus laevis, to examine plakophilin-3’s developmental roles. I first evaluated …

The Roles Of Phenotypic Plasticity And Genotypic Specialization In High Altitude Adaptation, Danielle M. Tufts

School of Biological Sciences: Dissertations, Theses, and Student Research

In vertebrates living at high altitude, arterial hypoxemia may be ameliorated by reversible changes in the oxygen-carrying capacity of the blood (regulated by erythropoiesis) and/or changes in blood–oxygen affinity (regulated by allosteric effectors of hemoglobin function). These hematological traits often differ between taxa that are native to different elevational zones, but it is often unknown whether the observed physiological differences reflect fixed, genetically based differences or environmentally induced acclimatization responses (phenotypic plasticity). Here, we report measurements of hematological traits related to blood–O₂ transport in populations of deer mice (Peromyscus maniculatus) that are native to high- and low-altitude …

Cytotoxicity In The Age Of Nano: The Role Of Fourth Period Transition Metal Oxide Nanoparticle Physicochemical Properties, Charles C. Chusuei, Chi-Heng Wu, Shravan Mallavarapu, Fang Yao Stephen Hou, Chen-Ming Hsu, Jeffrey G. Winiarz, Robert S. Aronstam, Yue-Wern Huang

Clinical Lab Sciences Faculty Research and Publications

A clear understanding of physicochemical factors governing nanoparticle toxicity is still in its infancy. We used a systematic approach to delineate physicochemical properties of nanoparticles that govern cytotoxicity. The cytotoxicity of fourth period metal oxide nanoparticles (NPs): TiO₂, Cr₂O₃, Mn₂O₃, Fe₂O₃, NiO, CuO, and ZnO increases with the atomic number of the transition metal oxide. This trend was not cell-type specific, as observed in non-transformed human lung cells (BEAS-2B) and human bronchoalveolar carcinoma-derived cells (A549). Addition of NPs to the cell culture medium did not significantly …

Incorporating New Technologies Into Toxicity Testing And Risk Assessment: Moving From 21st Century Vision To A Data-Driven Framework, Russell S. Thomas, Martin A. Philbert, Scott Auerbach, Barbara A. Wetmore, Michael J. Devito, Ila Cote, J. Craig Rowlands, Maurice Whelan, Sean M. Hays, Melvin E. Andersen, M.E. (Bette) Meek, Lawrence W. Reiter, Jason C. Lambert, Harvey J. Clewell Iii, Martin L. Stephens, Q. Jay Zhao, Scott C. Wesselkamper, Lynn Flowers, Edward W. Carney, Tim P. Pastoor, Dan D. Petersen, Carole L. Yauk, Andy Nong

in Vitro Research Models Collection

Based on existing data and previous work, a series of studies is proposed as a basis toward a pragmatic early step in transforming toxicity testing. These studies were assembled into a data-driven framework that invokes successive tiers of testing with margin of exposure (MOE) as the primary metric. The first tier of the framework integrates data from high-throughput in vitro assays, in vitro-to-in vivo extrapolation (IVIVE) pharmacokinetic modeling, and exposure modeling. The in vitro assays are used to separate chemicals based on their relative selectivity in interacting with biological targets and identify the concentration at which these interactions …