Laboratory and Basic Science Research Commons^™

A Novel Cyanopyridine Derived Fluorescent Sensor For Selective Determination Of Uranyl Ions In Different Water Samples, Ekram Hany Mohamed, Amany R Salem, Abeer M. El-Naggar,, Mohammed A. Amin,, Mohamed S. S. Attia

Pharmacy

A newly developed spectrofluorometric method is based on the fabrication of an optical cyanopyridine derived sensor, 4-(6-diaryl)-2-oxo -1,2-dihydropyridine-3-carbonitrile using microwave irradiation. The fluorescence intensity of the proposed chemo sensor was obviously quenched in DMSO owing to the presence of UO22+ ions at λex/λem = 320/360 nm. The quenching effect of the cyano-derivative based sensor was found to be proportional to the concentration of UO22+ ion ranging between 9.60 × 10–9–5.28 × 10–5 mol L−1 with a detection and quantification limits of 1.71 × 10–11 and 5.2 × 10–11 mol L−1, respectively. © 2021, Akadémiai Kiadó, Budapest, Hungary.

Chronic Adolescent Stress As A Predictive Factor For The Risk Of Developing Ptsd-Like Symptoms In Adulthood, Grace K. Young

Theses and Dissertations

Post-traumatic stress disorder is a stress and trauma based psychological disorder that is defined by the DSM-IV as an anxiety disorder that affects approximately 7.8% of people in the United States. PTSD is when those who suffer a traumatic event have intense and distressing feelings, emotions, and memories for a prolonged period of time after the event. A prominent feature of PTSD is the impaired ability to properly extinguish a fear response after a dangerous trigger or stressor is no longer present, also known as safety learning. Stressors are threats perceived within the environment that activate a response within the …

Regulation Of Skeletal Muscle Plasticity By The Gut Microbiome, Taylor R. Valentino

Theses and Dissertations--Physiology

Recent evidence suggests that the gut microbiome could play a role in skeletal muscle plasticity, providing novel treatments for muscle wasting diseases and/or performance enhancements. I first sought to determine if the gut microbiome is necessary for skeletal muscle adaptation to exercise. Forty-two, four-month old, female C57Bl/6J underwent nine weeks of weighted wheel running or remained in cage with a locked wheel, without or without the administration of antibiotics (treated). In response to wheel running, I found that antibiotic depletion of the microbiome led to a blunted hypertrophic response in the soleus muscle as measured by normalized muscle wet weight …

D-Dimer Testing In Covid-19: From Basics To Clinical Application, Bushra Moiz

Department of Pathology and Laboratory Medicine

No abstract provided.

Safety In The Woods: Studying The Effectiveness Of The Kentucky Master Logger Program, Austin Winn Leake

Theses and Dissertations--Community & Leadership Development

Logging is a dangerous field; in this study the researcher looks to see the knowledge both before and after professional loggers participate in the Kentucky Master Logger Program. This information was used to see the overall effectiveness of the Kentucky Master Logger Program. The researcher found an overall improvement in logging professionals’ perceptions and understanding of logging safety. The researcher recommends creating an emphasis on a safe work culture through safety incentives and a greater focus on less used personal protective equipment such as insecticide

Identifying And Minimizing Sources Of Variability Within Modern Spectroscopic Techniques For The Forensic Analysis Of Glass, Oriana Christy Ovide

Graduate Theses, Dissertations, and Problem Reports

Broken glass is a trace material frequently found at crime scenes such as hit-and-runs, burglaries, assaults, and homicides. Existing research encompassing the forensic analysis of glass evidence is vast. Published studies cover the analysis and interpretation of various types of glass. However, organizations such as the National Institute of Justice (NIJ), National Institute of Standards and Technology Organization of Scientific Area Committees (NIST-OSAC), and American Society of Trace Evidence Examiners (ASTEE) continue to identify glass-specific research needs to strengthen the scientific foundations of the field. Current gaps within the forensic glass community involve understanding modern glass formulations, re-evaluating the performance …

Tetramerization Of Stat5 Has Opposing Roles In Governing Autoimmune Pathogenesis, Kelly Lynn Monaghan

Graduate Theses, Dissertations, and Problem Reports

Signal transducers and activators of transcription 5 (STAT5A and STAT5B) are members of the STAT protein family. STAT5 plays a critical role in mediating cellular responses following cytokine stimulation. Critical STAT5 signaling is achieved following functional dimerization, which occurs via SH2 domain interactions. The binding of two STAT5 dimers to tandemly linked TTCN₃GAA γ-interferon-activated sequence (GAS) motifs and N-terminal domain interactions, facilitates functional tetramerization. The biological role of STAT5 tetramers has been investigated using the Stat5a-Stat5b N-domain double knock-in (DKI) mouse strain, in which STAT5 tetramers cannot be formed due to substitutions in critical amino acid …

The Rotator Cuff Tendon-To-Bone Interface: Maturation, Aging, And 3d Bioprinting For Regeneration, Xiping Jiang

Theses & Dissertations

Rotator cuff tendon injuries often occur at the tendon-to-bone interface (i.e., enthesis) area with a high prevalence for the elderly population. In addition, regeneration of the gradient structure of the enthesis is still a significant clinical challenge. Our studies aim to identify the histological, molecular, and biomechanical alterations of the rotator cuff enthesis with maturation and aging, and develop a novel therapeutic method using three-dimensional (3D) bioprinting technique to regenerate a functional enthesis. Striking variations of the entheses were observed both histologically and biomechanically during the maturation process. The histological features did not show many differences at the insertion site …

4d Ex Vivo Crispr/Cas9 Whole-Genome Screen To Identify Genes Regulating Lung Cancer Metastasis, Alexandria Plumer

Dissertations & Theses (Open Access)

Metastatic lung cancer has a 5-year survival rate of 5%. Lung cancers tend to be asymptomatic until late stages, and almost 90% are not diagnosed until they are advanced. Metastases are very rare events, often initiated by a single cell from a primary tumor into a new niche at a distant location. Investigation of the early metastatic process is of urgent need for the development of early diagnostics and targeted therapeutics. We performed a proof-of-concept CRISPR/Cas9 whole genome knockout screen in the A549 lung adenocarcinoma cell line and utilized a novel ex vivo 4D lung metastasis model to find gene …

Influence Of Saltwater Submergence On Geohydraulic Properties Of Sand: A Laboratory Investigation, Sudip Basack, Ghritartha Goswami, Sumanpran Sonowal, Moses Karakouzian

Civil and Environmental Engineering and Construction Faculty Research

Saline water intrusion into freshwater aquifers is a major geohydraulic problem relevant to coastal environment. Apart from contaminating the fresh groundwater resources, the saltwater intrusion alters the geotechnical properties of the aquifer materials, affecting the coastal water resource planning and management. The present study focuses on an in-depth laboratory investigation of the influence of saltwater submergence on the geohydraulic properties of sand. The fine sand sample was submerged under saline water of specified concentrations for specific periods, and the alteration in their engineering properties has been studied. It is observed that the specific gravity, dry density, and permeability of fine …

Differentiating The Mechanistic Role And Chemotherapeutic Potential Of Src And Podoplanin In Oncogenic Transformation, Edward P. Retzbach

Graduate School of Biomedical Sciences Theses and Dissertations

There were an estimated 20 million new cancer cases worldwide in 2020, resulting in nearly 1000 deaths per hour [1]. Oral cancer exemplifies the difficulties of treating cancer patients. The first line for oral cancer treatment is surgery and radiation that can lead to patient disfigurement and decreased quality of life in cancer survivors [2-4]. Though there have been many developments in chemotherapy in the last 30 years, the 50% mortality rate associated with oral cancer has not changed [4, 5]. Longitudinal studies that track survival rates in oral cancer patients demonstrate a 3-fold reduction in patient deaths when patients …

Effect Of Hydrogen Peroxide On Algae And Microcystin In Control And Lake Waters, Tatiana Castillo Hernandez

Biological and Agricultural Engineering Undergraduate Honors Theses

Cyanobacteria are photo-autotrophic organisms with a worldwide distribution, which can result in Harmful Algal Blooms (HABs) producing toxins. One of the most common strains of cyanobacteria is Microcystis, which produces the most abundant cyanotoxin, microcystin. In this study, we analyzed the effect of H₂O₂ on algae and microcystin using both lake and reagent grade water. The first objective was to determine the effect of H₂O₂ on algae and cyanobacteria in lake water that was nutrient enriched. The second objective was to detect the effect of H₂O₂ at oxidizing microcystin in …

The Role Of The Erbb Signaling Pathway In Cardiovascular Progenitor Cell-Based Repair, Christopher Ramos

Electronic Theses, Projects, and Dissertations

Adult mammalian hearts lack self-renewal and proliferative capabilities necessary for cardiovascular regeneration. Current treatments using cardiac progenitor cells (CPCs) for cell-based repair do not restore cardiac function in patients who experience a myocardial infarction (MI). Our laboratory has been studying Islet-1+ neonatal CPCs as a promising candidate for cell-based repair due to their ability to significantly improve cardiac function after MI in sheep. The current study addresses the hypothesis that the ERBB pathway is linked to the Hippo-pathway to activate YAP1 by the involvement of an autocrine loop that upregulates neuregulin (NRG). In our sheep model of MI and cardiovascular …

Drivers Of Extracellular Polysaccharide Production By A Mat-Forming Diatom, Kaitlin A. Stansbury

FIU Electronic Theses and Dissertations

Microbial biofilms are held together by extracellular polymeric substances (EPS), which can be secreted by many organisms. EPS production can facilitate intercellular communication and inter-guild microbial mutualisms, intraspecific gamete exchange, nutrient sequestration, and desiccation resistance. Benthic microbial mats (periphyton) of the Florida Everglades and other karstic wetlands contain abundant EPS generated by mat-producing filamentous blue-green algae and many other species of mat-dwelling algae, cyanobacteria, and bacteria, including the most abundant Everglades diatom, Mastagloia calcarea. The benthic diatom genus Mastogloia is characterized by several morphological and physiological features that foster production of a ‘halo’ of EPS around the frustule, but the …

Laboratory Research Skills, Joanna Burkhardt

Library Impact Statements

No abstract provided.

Laboratory Research Skills (Permanent), Joanna Burkhardt

Library Impact Statements

No abstract provided.

Reproducibility Of Alkaline Inorganic Phosphate Quantification Using 31p-Magnetic Resonance Spectroscopy At 3t, Alexs A. Matias

Masters Theses

INTRODUCTION: The detection of a second inorganic phosphate (Pi) resonance, a possible marker of mitochondrial content in vivo, using phosphorus magnetic resonance spectroscopy (31P- MRS) at 3T is technically challenging, which may prevent its reproducible quantification. PURPOSE: To determine the reproducibility of resting alkaline inorganic phosphate (Pialk) measurement using 31P-MRS in human skeletal muscle at 3 tesla (T). METHODS: Resting 31P- MRS of the quadriceps muscles was acquired on two separate visits, within seven days, in 13 healthy, sedentary to moderately active young adults using a whole-body 3T MR system. Measurement variability related to coil position, shimming procedure, and spectral …

Motor Unit Firing Rate Control Of Agonist Skeletal Muscle During Voluntary Isometric And Shortening Contractions With Limb Movement, Eric A. Kirk

Electronic Thesis and Dissertation Repository

Voluntary movements throughout mammalian lifespan require skeletal muscle contractions that are controlled by time- and recruitment-dependent firing rate patterns. Single motor unit (MU) activity reflects the final efferent neural drive to the muscle, yet the underlying neural control of movement at the MU level is not well understood. Using intramuscular electromyography single MU recordings, relationships between voluntary contraction kinematics and MU firing rates were evaluated in vivo, in groups of young and older adult participants.

The purpose of study one was to characterize how MU firing rates are differently scaled among muscles relative to voluntary contraction intensity. Across 12 …

Exogenous Surfactant As A Delivery Vehicle For Intrapulmonary Therapeutics, Brandon J. Baer

Electronic Thesis and Dissertation Repository

As an organ system, the lung has unique advantages and disadvantages for direct drug delivery. Its contact with the external environment allows for the airways to be easily accessible to intrapulmonary delivery. However, its complex structure, which divides into more narrow airways with each branch, can make direct delivery to the remote alveoli challenging. The objective of this thesis was to overcome this issue by using exogenous surfactant, a lipoprotein complex used to treat neonatal respiratory distress syndrome, as a carrier for pulmonary therapeutics. It was hypothesized that therapeutics administered with a surfactant vehicle would display enhanced delivery to the …

Tb3+-Atorvastatin Doped In Poly(Ethylene Glycol) Optical Biosensor For Selective Determination Of Progesterone And Testosterone In Serum Samples, Ekram Hany Mohamed, Mohamed S. S. Attia, Safwat A. Mahmoud,, Amal M. Ahmed,, Tarek A. Amin, Ahmed O. Youssef, Mona N. Abou-Omar,, Mohammed A. C Amin,

Pharmacy

An innovative, simple and cost effective Tb3+-atorvastatin photo probe was designed and used as a core for a spectrofluorometric approach to sensitively determine two vital biological compounds in serum samples. Tb3+-atorvastatin complex displays a characteristic electrical band with λem at 545 nm with significant luminescence intensity, which is quenched in the presence of progesterone and testosterone at two variant sets of pH; 6.2 and 7.5, respectively. The conditions were optimized and the best solvent for operation was found to be acetonitrile with λex at 320 nm. Progesterone and testosterone were assessed in serum samples using the same optimal conditions within …