Entomology Commons^™

Comparison Of The Effects Of Extracts From Three Vitex Plant Species On Anopheles Gambiae S.S. (Diptera: Culicidae) Larvae, Mokua G. Nyamoita, Innocent Ester, Mbwambo H. Zakaria, Lwande Wilber, Ochola J. Bwire, Hassanali Ahmed

All Peer-Reviewed Publications

Acetone and methanol extracts of different parts of three Vitex species (leaves and stem bark of Vitex trifolia, leaves, stem bark and root bark of Vitex schiliebenii and stem and root bark of Vitex payos) were evaluated for their potential to control Anopheles gambiae Giles s.s. larvae (Diptera: Culicidae). The extracts gave different levels and rate of mortality of the larvae. Some (methanol extract of V. trifolia leaves, acetone extracts of stem bark and leaves of V. schiliebenii, acetone extract of root bark of V. payos) caused 100% mortality at 100ppm in 72h, with those of V. schiliebenii and V. …

Occurrence, Diversity And Pattern Of Damage Of Oplostomus Species (Coleoptera: Scarabaeidae), Honey Bee Pests In Kenya, Ayuka T. Fombong, Fiona N. Mumoki, Elliud Muli, Daniel K. Masiga, Richard T. Arbogast, Peter E.A. Teal, Baldwyn Torto

All Peer-Reviewed Publications

Several arthropod pests including the hive beetles Aethina tumida and Oplostomus haroldi and the ectoparasite Varroa destructor have recently been identified as associated with honey bee colonies in Kenya. Here, we report the first documentation of Oplostomus fuligineus in Kenya, a related scarab of O. haroldi, and distribution, diversity and pattern of damage of the two scarab species on honey bee colonies. Sequence analyses of mitochondrial cytochrome oxidase I gene revealed that there was sufficient sequence divergence to separate both Oplostomus beetles. The same molecular marker separated O. haroldi according to place of origin in Kenya. We further show from …

Development Of A Gravid Trap For Collecting Live Malaria Vectors Anopheles Gambiae S.L, Sisay Dugassa, Jenny M. Lindh, Florence Oyieke, Wolfgang R. Mukabana, Steven W. Lindsay, Ulrike Fillinger

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BACKGROUND: Effective malaria vector control targeting indoor host-seeking mosquitoes has resulted in fewer vectors entering houses in many areas of sub-Saharan Africa, with the proportion of vectors outdoors becoming more important in the transmission of this disease. This study aimed to develop a gravid trap for the outdoor collection of the malaria vector Anopheles gambiae s.l. based on evaluation and modification of commercially available gravid traps. METHODS: Experiments were implemented in an 80 m(2) semi-field system where 200 gravid Anopheles gambiae s.s. were released nightly. The efficacy of the Box, CDC and Frommer updraft gravid traps was compared. The Box …

Development Of Integrated Pest Management For Sugarcane Borer, Diatraea Saccharalis In Rice, Jaspreet Kaur Sidhu

LSU Doctoral Dissertations

Rice is grown over an area of approximately 500,000 acres in Louisiana. The lepidopteran stem borer complex attacking rice in the southern U.S includes stalk borer Chilo plejadellus Zincken, sugarcane borer (SCB) Diatraea saccharalis (F.) and Mexican rice borer, Eoreuma loftini Dyar. With the increasing impact of stem borers in Louisiana, an urgent need exists to develop strategies for management. Currently, no IPM program is in place for stem borers in Louisiana rice and research has been initiated to develop an IPM program for these pests. The first objective of this research was focused on host plant resistance. For this …

Taxonomy And Systematics Of New Zealand Faronitae (Coleoptera: Staphylinidae: Pselaphinae), Jong-Seok Park

LSU Doctoral Dissertations

The New Zealand Faronitae was revised at the species level, and a phylogenetic study was performed based on morphological and molecular data. Eight new genera and 143 new species were discovered. Three new genera, “Brounea”, “Ahnea” and “Pseudoexeirarthra” were designated based on the species originally described in the genus Sagola. Five new genera, “Aucklandea”, “Chandlerea”, “Nunnea”, “Leschenea” and “Pseudostenosagola” were designated by new species. Three known genera, Exeirarthra, Stenosagola and Sagola were also revised at species level. Eighty four names were synonymized, two in Exeirarthra, four in Stenosagola and 78 in Sagola. Lectotypes of seven species, Sagola terricola Broun, S. …

First Record Of Calophya Schini (Hemiptera: Calophyidae) In Ethiopia And Kenya, William A. Overholt, Robert S. Copeland, Susan E. Halbert

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A South American leaf galling psyllid, Calophya schini Tuthill, has recently been discovered attacking the South American tree Schinus molle L. in Kenya and Ethiopia. This represents the first record of the psyllid in each country and the only record of C. schini in Africa outside of South Africa. In Kenya, the psyllid was parasitized by an unidentified tetrastichine (Eulophidae), probably of the genus Tamarixia, while in Ethiopia C. schini nymphs were heavily parasitized by an unidentified hymenopteran. Copyright © icipe 2013.

Comparison Of Volatile Blends And Nucleotide Sequences Of Two Beauveria Bassiana Isolates Of Different Virulence And Repellency Towards The Termite Macrotermes Michealseni, D. M. Mburu, N. K. Maniania, A. Hassanali

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Isolates of the fungus Beauveria bassiana have different levels of virulence and repellency against the termite Macrotermes michaelseni. In the present study, we compared the volatile profiles and gene sequences of two isolates of the fungus with different levels of virulence and repellence to the termite. Gas chromatography-mass spectrometric analyses showed quantitative and qualitative differences in the composition of volatiles of the two isolates. The repellencies of synthetic blends of 10 prominent components that mimicked the volatiles of each of the two isolates were significantly different. Subtractive bioassays showed that the repellency of each isolate was due to synergistic effects …

Common Host-Derived Chemicals Increase Catches Of Disease-Transmitting Mosquitoes And Can Improve Early Warning Systems For Rift Valley Fever Virus, David P. Tchouassi, Rosemary Sang, Catherine L. Sole, Armanda D.S. Bastos, Peter E.A. Teal, Christian Borgemeister, Baldwyn Torto

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Rift Valley fever (RVF), a mosquito-borne zoonosis, is a major public health and veterinary problem in sub-Saharan Africa. Surveillance to monitor mosquito populations during the inter-epidemic period (IEP) and viral activity in these vectors is critical to informing public health decisions for early warning and control of the disease. Using a combination of field bioassays, electrophysiological and chemical analyses we demonstrated that skin-derived aldehydes (heptanal, octanal, nonanal, decanal) common to RVF virus (RVFV) hosts including sheep, cow, donkey, goat and human serve as potent attractants for RVFV mosquito vectors. Furthermore, a blend formulated from the four aldehydes and combined with …

Current Known Range Of The Platte River Caddisfly, Ironoquia Plattensis, And Genetic Variability Among Populations From Three Nebraska Rivers, Lindsay A. Vivian, Michael Cavallaro, Kate Kneeland, Erica Lindroth, W. Wyatt Hoback, Kerri M. Farnsworth-Hoback, Robert R. Harms, John E. Foster

Department of Entomology: Faculty Publications

The Platte River caddisfly (Ironoquia plattensis Alexander and Whiles 2000) was recently described from a warm-water slough along the Platte River in central Nebraska and was considered abundant at the type locality. Surveys of 48 sites in 1999 and 2004 found eight additional sites with this species on the Platte River. The caddisfly was not found at the type locality in 2004 and one additional site in 2007, presumably because of drought conditions. Because of its apparent rarity and decline, the Platte River caddisfly is a Tier I species in Nebraska. For this project, surveys for the caddisfly were …

Dietary Supplementation With Pollen Enhances Survival And Collembola Boosts Fitness Of A Web-Building Spider, Jason M. Schmidt, Julie A. Peterson, Jonathan Lundgren, James D. Harwood

Department of Entomology: Faculty Publications

Uncertainties exist about the value of non-prey food for predators that are commonly food-limited, and the dietary conditions where non-prey foods are beneficial for carnivorous species. Prior studies show that large quantities of pollen grains are intercepted in the webs of web-building spiders. We examined the nutritional benefits of pollen as a non-prey food for a common ground-dwelling, sheet web-building spider, Mermessus fradeorum (Berland) (Araneae: Linyphiidae). These predators were provided diets of prey or no prey in the presence and absence of pollen. Treatment effects were quantified by measuring predator body nutrient composition, survival, body size, and offspring production. Per …

Bacillus Thuringiensis: Transgenic Crops, Julie A. Peterson, John J. Obrycki, James D. Harwood

Department of Entomology: Faculty Publications

Bacillus thuringiensis (Bt) crops, genetically modified to express insecticidal toxins that target key pests of corn, cotton, rice, potato, and other crops, have been rapidly adopted and have become dominant fixtures in agroecosystems throughout the world. Due to the constitutive nature of Bt toxin expression, insecticidal proteins may be found in nearly all plant tissues, presenting multiple sources for Bt toxins to enter the environment, thus creating complex direct and indirect pathways for non-target organisms to be exposed to insecticidal proteins. The environmental impacts of Bt crops have been widely debated, although both benefits and risks do exist. Benefits of …

Compositions And Methods For Synergistic Manipulation Of Plant And Insect Defenses, Graham Moores, Georgina Bingham

Department of Entomology: Faculty Publications

This invention relates to the control of plant pests, such as aphid and whitefly by treating plants with a compound which inhibits the plant pests ability to overcome plant defense responses, such as piperonyl butoxide or propyl gallate, in combination with a compound which activates plant defense responses, such as cis-Jasmone or beta-aminobutyric acid.

Doublesex Target Genes In The Red Flour Beetle, Tribolium Castaneum, Jayendra Nath Shukla, Subba Reddy Palli

Entomology Faculty Publications

Sex determination cascade in insects terminates with the production of sex-specific protein, Doublesex (Dsx). We identified the dsx homolog (Tcdsx) in Tribolium castaneum. The pre-mRNA of Tcdsx is sex-specifically spliced into three female (Tcdsxf1, Tcdsxf2 and Tcdsxf3) and one male-specific (Tcdsxm) isoforms. Cis-regulatory elements potentially involved in sex-specific splicing of the Tcdsx pre-mRNA were identified in the female-specific exon and the adjoining intronic sequences. All the three female-specific TcDsx proteins share common OD1 and OD2 domains and differ in their C-terminal sequences. Knockdown of Tcdsx resulted in a reduction in the oocyte development, egg production and hatching of eggs laid. …

Reactive Oxygen Species Production And Brugia Pahangi Survivorship In Aedes Polynesiensis With Artificial Wolbachia Infection Types, Elizabeth S. Andrews, Philip R. Crain, Yuqing Fu, Daniel K. Howe, Stephen L. Dobson

Entomology Faculty Publications

Heterologous transinfection with the endosymbiotic bacterium Wolbachia has been shown previously to induce pathogen interference phenotypes in mosquito hosts. Here we examine an artificially infected strain of Aedes polynesiensis, the primary vector of Wuchereria bancrofti, which is the causative agent of Lymphatic filariasis (LF) throughout much of the South Pacific. Embryonic microinjection was used to transfer the wAlbB infection from Aedes albopictus into an aposymbiotic strain of Ae. polynesiensis. The resulting strain (designated "MTB") experiences a stable artificial infection with high maternal inheritance. Reciprocal crosses of MTB with naturally infected wild-type Ae. polynesiensis demonstrate strong bidirectional incompatibility. Levels of reactive …

Investigations On Rangeland Grasshoppers: Ecoregion Level Distribution, Identification, Feeding Performance, And Vegetation Clipping, Muhammad Ullah

Department of Entomology: Dissertations, Theses, and Student Research

Short-horned grasshoppers (Orthoptera: Acrididae) were studied in the field as well as in laboratory for their distribution, identification, preferred food plants and feeding damage. These grasshoppers are important potential pests of rangelands where they compete for forage with cattle. Examination of the 20 most common grasshoppers by levels 3 and 4 of Nebraska ecoregions revealed specific habitat use and may be helpful in predicting hotspots. The relative abundance of all species analyzed showed at least some significant differences at ecoregion level 4 and two species showed single ecoregion that differed from all others at level 3.

Grasshoppers belonging to subfamilies …

Chemical Communication In The Honey Bee Scarab Pest Oplostomus Haroldi: Role Of (Z)-9-Pentacosene, Ayuka T. Fombong, Peter E.A. Teal, Richard T. Arbogast, Paul N. Ndegwa, Lucy W. Irungu, Baldwyn Torto

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Oplostomus haroldi Witte belongs to a unique genus of afro-tropical scarabs that have associations with honey bee colonies, from which they derive vital nutrients. Although the attributes of the honey bee nest impose barriers to communication among nest invaders, this beetle still is able to detect conspecific mates for reproduction. Here, we show, through behavioral studies, that cuticular lipids serve as mate discrimination cues in this beetle. We observed five steps during mating: arrestment, alignment, mounting, and copulation, and a post-copulatory stage, lasting ~40-70 % of the total mating duration, that suggested mate guarding. Chemical analysis identified the same nine …

Farmers' Perceptions Of Cotton Pests And Their Management In Western Kenya, Charles A.O. Midega, Isaac M. Nyang'au, Jimmy Pittchar, Michael A. Birkett, John A. Pickett, Miguel Borges, Zeyaur R. Khan

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Cotton (Gossypium hirsutum L.) production in western Kenya is severely affected by insect pests, resulting in poor yields in spite of the growing demand for the commodity. One of the key constraints to establishing effective pest management approaches for smallholder farmers is the lack of adequate information about farmers' knowledge, perceptions and practices in pest management. This study was therefore conducted to (1) evaluate farmers' knowledge and perceptions of cotton pests; (2) examine farmers' current practices in managing cotton pests; (3) identify pest management challenges and intervention opportunities in order to develop efficient integrated pest management (IPM) approaches. A total …

Semiochemicals From Herbivory Induced Cotton Plants Enhance The Foraging Behavior Of The Cotton Boll Weevil, Anthonomus Grandis, D. M. Magalhães, M. Borges, R. A. Laumann, E. R. Sujii, P. Mayon, J. C. Caulfield, C. A.O. Midega, Z. R. Khan, J. A. Pickett, M. A. Birkett, M. C. Blassioli-Moraes

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The boll weevil, Anthonomus grandis, has been monitored through deployment of traps baited with aggregation pheromone components. However, field studies have shown that the number of insects caught in these traps is significantly reduced during cotton squaring, suggesting that volatiles produced by plants at this phenological stage may be involved in attraction. Here, we evaluated the chemical profile of volatile organic compounds (VOCs) emitted by undamaged or damaged cotton plants at different phenological stages, under different infestation conditions, and determined the attractiveness of these VOCs to adults of A. grandis. In addition, we investigated whether or not VOCs released by …

Species Richness And Nest Dispersion Of Some Tropical Meliponine Bees (Apidae: Meliponinae) In Six Habitat Types In The Kakamega Forest, Western Kenya, Kiatoko Nkoba, Suresh Kumar Raina, Elluid Muli, Klaus Mithofer, Jones Mueke

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A study to determine the species richness and spatial distribution of five meliponine bee species in three different habitats in the Kakamega forest was undertaken for the first time. Two forest (indigenous and mixed indigenous) and two grassland (with Eucalyptus spp. trees and indigenous trees) types, along with homesteads (in the vicinity of mixed indigenous forest and indigenous forest, respectively), were surveyed. Line transect methods were used in the nest survey in each habitat. The highest number of species was recorded in the indigenous forest, while no nest was discovered in the grassland with Eucalyptus spp. trees. The mean number …

Disentangling Dispersal, Vicariance And Adaptive Radiation Patterns: A Case Study Using Armyworms In The Pest Genus Spodoptera (Lepidoptera: Noctuidae), Gael J. Kergoat, Dorothy P. Prowell, Bruno P. Le Ru, Andrew Mitchell, Pascaline Dumas, Anne Laure Clamens, Fabien L. Condamine, Jean François Silvain

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Thanks to the recent development of integrative approaches that combine dated phylogenies with models of biogeographic evolution, it is becoming more feasible to assess the roles of dispersal and vicariance in creating complex patterns of geographical distribution. However, the historical biogeography of taxa with good dispersal abilities, like birds or flying insects, still remains largely unknown because of the lack of complete phylogenies accompanied by robust estimates of divergence times. In this study, we investigate the evolution and historical biogeography of the globally distributed pest genus Spodoptera (Lepidoptera: Noctuidae) using complete taxon sampling and an extensive set of analyses. Through …