Entomology Commons^™

Vector Competence Of Aedes Aegypti Populations From Kilifi And Nairobi For Dengue 2 Virus And The Influence Of Temperature, Edith Chepkorir, Joel Lutomiah, James Mutisya, Francis Mulwa, Konongoi Limbaso, Benedict Orindi, Zipporah Ng'ang'a, Rosemary Sang

All Peer-Reviewed Publications

Background: Susceptibility of Ae. aegypti mosquito to dengue virus (DENV) varies geographically and can be influenced by climatic factors such as temperature, which affect the incidence, seasonality and distribution of vector-borne diseases. The first outbreak of dengue fever (DF) in Kenya occured in 1982 in the coastal towns of Malindi and Kilifi. Unlike Nairobi where no active dengue transmission has been reported, DF is currently re-emerging at the Coast causing major outbreaks. This study investigated the vector competence of Ae. aegypti populations from two urban areas, Kilifi (Coast of Kenya) and Nairobi (Central Kenya), for DEN-2 virus and the influence …

Organic Treatments For Control Of Pepper Weevil (Coleoptera: Curculionidae), Karla M. Addesso, Philip A. Stansly, Barry C. Kostyk, Heather J. Mcauslane

Agricultural and Environmental Sciences Faculty Research

The pepper weevil, Anthonomus eugenii Cano (Coleoptera: Curculionidae) is a major pest of pepper (Capsicum spp.; Solanales: Solanaceae) in the southern United States, Mexico, Central America and the Caribbean. Feeding and oviposition cause flower and fruit abscission and internal fruit damage resulting in serious yield losses. Females lay eggs in flower buds and small fruits, shielding larvae from contact pesticides, leaving only the adult stage vulnerable. The purpose of this study was to investigate low-risk and organic products for use against the pepper weevil to provide both organic and conventional growers with more control options. A neem product (Ecozin® …

Nebline, September 2014

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: 4-H Youth Showcase Their Achievements at Super Fair

Food & Health

Family Living

Farm & Acreage

Home & Family Living

Horticulture

Pests & Wildlife

4-H & Youth

Extension Calendar

and other extension news and events

Influence Of Resource Levels, Organic Compounds And Laboratory Colonization On Interspecific Competition Between The Asian Tiger Mosquito Aedes Albopictus (Stegomyia Albopicta) And The Southern House Mosquito Culex Quinquefasciatus, D.W. Allgood, Donald A. Yee

Faculty Publications

The mosquitoes Aedes albopictus (Stegomyia albopicta) (Skuse) and Culex quinquefasciatus (Say) (Diptera: Culicidae) are common inhabitants of tyres and other artificial containers, which constitute important peridomestic mosquito breeding habitats. We tested the hypotheses that interspecific resource competition between the larvae of these species is asymmetrical, that the concentration of chemicals associated with decomposing detritus affects the competitive outcomes of these species, and that wild and colonized strains of Cx. quinquefasciatus are affected differently by competition with Ae. albopictus. We conducted two laboratory competition experiments wherein we measured survivorship and estimated population growth (λ′) in both species under …

Factors Limiting The Spread Of The Protective Symbiont Hamiltonella Defensa In Aphis Craccivora Aphids, Hannah R. Dykstra, Stephanie R. Weldon, Adam J. Martinez, Jennifer A. White, Keith R. Hopper, George E. Heimpel, Mark K. Asplen, Kerry M. Oliver

Entomology Faculty Publications

Many insects are associated with heritable symbionts that mediate ecological interactions, including host protection against natural enemies. The cowpea aphid, Aphis craccivora, is a polyphagous pest that harbors Hamiltonella defensa, which defends against parasitic wasps. Despite this protective benefit, this symbiont occurs only at intermediate frequencies in field populations. To identify factors constraining H. defensa invasion in Ap. craccivora, we estimated symbiont transmission rates, performed fitness assays, and measured infection dynamics in population cages to evaluate effects of infection. Similar to results with the pea aphid, Acyrthosiphon pisum, we found no consistent costs to infection using …

Life History Of Paracoccus Marginatus (Hemiptera: Pseudococcidae) On Four Host Plant Species Under Laboratory Conditions, Kaushalya G. Amarasekare, Catharine M. Mannion, Lance S. Osborne, Nancy D. Epsky

Agricultural and Environmental Sciences Faculty Research

Life history of the mealybug, Paracoccus marginatus Williams and Granara de Willink, on three ornamental plants [Hibiscus rosa-sinensis L., Acalypha wilkesiana (Muell.-Arg.), and Plumeria rubra L.] and one weed species (Parthenium hysterophorus L.) was studied under laboratory conditions. Mealybugs were able to develop, survive, and reproduce on all four hosts; however, there were differences in the life history parameters. Adult females that developed on acalypha and parthenium emerged ≈1 d earlier than those that developed on hibiscus and plumeria. Adult males had a longer developmental time on plumeria than on the other hosts. Survival of first- and second-instar …

Sma-Causing Missense Mutations In Survival Motor Neuron (Smn) Display A Wide Range Of Phenotypes When Modeled In Drosophila, Kavita Praveen, Ying Wen, Kelsey M. Gray, John J. Noto, Akash R. Patlolla, Gregory D. Van Duyne, A. Gregory Matera

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Mutations in the human survival motor neuron 1 (SMN) gene are the primary cause of spinal muscular atrophy (SMA), a devastating neuromuscular disorder. SMN protein has a well-characterized role in the biogenesis of small nuclear ribonucleoproteins (snRNPs), core components of the spliceosome. Additional tissue-specific and global functions have been ascribed to SMN; however, their relevance to SMA pathology is poorly understood and controversial. Using Drosophila as a model system, we created an allelic series of twelve Smn missense mutations, originally identified in human SMA patients. We show that animals expressing these SMA-causing mutations display a broad range of …

Molecular Xenomonitoring Using Mosquitoes To Map Lymphatic Filariasis After Mass Drug Administration In American Samoa, Mark A. Schmaedick, Amanda L. Koppel, Nils Pilotte, Melissa Torres, Steven A. Williams, Stephen L. Dobson, Patrick J. Lammie, Kimberly Y. Won

Entomology Faculty Publications

BACKGROUND: Mass drug administration (MDA) programs have dramatically reduced lymphatic filariasis (LF) incidence in many areas around the globe, including American Samoa. As infection rates decline and MDA programs end, efficient and sensitive methods for detecting infections are needed to monitor for recrudescence. Molecular methods, collectively termed 'molecular xenomonitoring,' can identify parasite DNA or RNA in human blood-feeding mosquitoes. We tested mosquitoes trapped throughout the inhabited islands of American Samoa to identify areas of possible continuing LF transmission after completion of MDA.

METHODOLOGY/PRINCIPLE FINDINGS: Mosquitoes were collected using BG Sentinel traps from most of the villages on American Samoa's largest …

Nebline, August 2014

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: Cherry Creek Habitat Provides Opportunities for Pollinator Education

Food & Health

Family Living

Farm & Acreage

Home & Family Living

Horticulture

Pests & Wildlife

4-H & Youth

Extension Calendar

and other extension news and events

Special pullout section: Lancaster County Super Fair 2014 Schedule & Map

Hexapod Herald - Vol. 26, No. 4, August 2014

Hexapod Herald and Other Entomology Department Newsletters

Contents: Welcome - Congratulations - Faculty News - Publications - Grants - Travel - Meet an Off-Campus Student - Student News

Repair Of The Proboscis Of Brush-Footed Butterflies (Lepidoptera: Nymphalidae), Suellen Pometto

All Theses

A key feature of the order Lepidoptera is the coilable proboscis, present in over 99% of lepidopteran species. The proboscis is used to obtain liquid nutrition, usually floral nectar. The proboscis is assembled from two elongate galeae immediately after emergence of the adult from the pupa. What happens if the galeae become separated? I studied the process of repair of the proboscis, behaviorally and functionally, at the organismal level. My research questions were as follows: 1) is the proboscis capable of repair, 2) is saliva necessary to proboscis repair, and 3) is the repaired proboscis able to acquire fluids? Test …

Rna Interference As A Tool For The Functional Analysis Of Genes In The Colorado Potato Beetle, Leptinotarsa Decemlineata (Say), Ashley Danielle Yates

Department of Entomology: Dissertations, Theses, and Student Research

RNA interference (RNAi) is a naturally occurring phenomenon in eukaryotes in which a double-stranded RNA (dsRNA) suppresses the expression of a target gene. RNAi has markedly changed the way in which functional genetics studies are performed, especially in non-model organisms. In insects, the efficacy of RNAi is influenced by several factors, including the species and the methods of dsRNAs delivery.

The Colorado potato beetle (CPB), Leptinotarsa decemlineata (Say), is a pest of the plant family Solanaceae. RNAi in the CPB is of interest for potential use in insect management and as a tool to study the interaction with host plants. …

Characterization Of Greenbug Feeding Behavior And Aphid (Hemiptera: Aphididae) Host Preference In Relation To Resistant And Susceptible Tetraploid Switchgrass Populations, Kyle G. Koch, Nathan Palmer, Mitch Stamm, Jeff D. Bradshaw, Erin Blankenship, Lisa M. Baird, Gautam Sarath, Tiffany M. Heng-Moss

Department of Entomology: Faculty Publications

Two choice studies were performed to evaluate greenbug, Schizaphis graminum (Rondani), and yellow sugarcane aphid, Sipha flava (Forbes), preference for two tetraploid switchgrass populations, Summer and Kanlow, and one experimental hybrid, K×S, derived by crossing Kanlow (male)×Summer (female) plants. Additionally, an assessment of S. graminum feeding behavior was performed on the same switchgrass populations, by using the electrical penetration graph (EPG) technique. Choice studies for S. flava indicated a lack of antixenosis, with no preference by aphids among any of the switchgrass populations at any time point. However, choice studies with S. graminum indicated a preference for the K×S plants …

Host Specificity And Ectoparasite Load Of Bat Flies In Utila, Honduras, Courtney Miller

Senior Honors Theses

Bat flies (Streblidae) are obligate blood-feeding ectoparasites of bats that display varying degrees of host specificity. A total of 265 streblid bat flies were collected from 122 bats belonging to the families Phyllostomidae and Natalidae from Utila, the smallest bay island of Honduras. Out of four host-parasite associations, three were considered primary. Out of the three bat species analyzed, one had significantly lower parasite prevalence and another had significantly higher parasite load and intensity. Both male and female bats were equally likely to be infested and variables of parasite density did not differ amongst host sex for any species. However, …

Similar Differentiation Patterns Between Pbp Expression Levels And Pheromone Component Ratios In Two Populations Of Sesamia Nonagrioides, Nicolas Glaser, Brigitte Frérot, Ene Leppik, Christelle Monsempes, Claire Capdevielle-Dulac, Bruno Le Ru, Thomas Lecocq, Myriam Harry, Emmanuelle Jacquin-Joly, Paul Andre Calatayud

All Peer-Reviewed Publications

Pheromone-binding proteins (PBPs) are thought to contribute to the specificity of the pheromone detection system through an initial selective binding with pheromone molecules. Here, we report different expression levels of PBP transcripts in the antennae of two populations of the stemborer Sesamia nonagrioides (Lepidoptera: Noctuidae), one collected in Europe and one in sub-Saharan Africa. The three PBP transcripts previously identified in this species were found to be expressed in both male and female antennae. Whereas PBP3 did not show any differential expression, PBP1 and PBP2 appeared to be expressed differently according to the population origin and sex. Simultaneously, we measured …

Push-Pull: A Novel Ipm Strategy For The Green Revolution In Africa, Zeyaur R. Khan, Charles A.O. Midega, Jimmy O. Pittchar, John A. Pickett

All Peer-Reviewed Publications

Africa faces serious challenges in feeding its population, mainly due to poor yields of cereals that serve as both staple and cash crops occasioned by insect pests, weeds, and poor soil fertility, and more recently effects of climate change. A novel IPM approach dubbed push-pull has been developed and implemented in eastern Africa, based on locally available companion plants, that effectively addresses these constraints resulting in substantial grain yield increases. The technology involves intercropping cereal crops with stemborer moth repellent crops (push), the forage legume desmodium or molasses grass, and planting the attractive Napier grass (pull) as a border crop. …

An Evaluation Of Watermelon (Citrullus Spp.) Germplasm For Additional Sources Of Resistance To The Twospotted Spider Mite (Tetranychus Urticae Koch)., Hector Cantu Jr.

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Fourteen U.S plant introduction (PI) accessions of C. lanatus var. lanatus (4), C. lanatus var. citroides (5) C. colocynthis (5) and a known susceptible commercial cultivar ‘Sugar Baby’ were evaluated for resistance to the twospotted spider mite, Tetranychus urticae Koch, in a limited free-choice and free-choice bioassay under laboratory conditions. The limited free choice bioassay, involved nine Petri dish cages that held five randomly assigned leaves individually inoculated with two adult females and one adult male. Eggs, larva, and adults were counted over a nine day period. The free choice bioassay involved the even distribution of three mite infested …

Evaluating Green Stink Bug Damage And Insect Abundance In Edamame, Benjamin Carroll Thrash

Graduate Theses and Dissertations

Edamame are an emerging crop in Arkansas, with 2013 planting estimates at 680 hectares. Although edamame are the same species as conventional soybeans grown in Arkansas, differences in use, maturity, and harvest may require creation and implementation of insect pest management approaches that differ from those used in conventional soybean production systems in order for a quality crop to be produced. Studies were conducted to determine a green stink bug damage-density relationship for edamame. Cages were used to confine green stink bugs on field-planted edamame at densities of 0, 2, 6, or 12 green stink bugs per cage (0, 0.55, …

Potential Interactions Of Early Season Herbicides And Insecticides In Cotton: Thrips Control And Plant Health, Derek Lane Clarkson

Graduate Theses and Dissertations

Cotton growth early in the season is affected by damaging levels of thrips in many production fields in Arkansas. During this time, insecticides used to control thrips and herbicides used to control weeds are often present at the same time on the cotton plant. This research explores how various combinations of herbicides and insecticides influence thrips numbers and cotton plant growth parameters early in the growing season. Pathways evaluated included interactions between preemergence herbicides and insecticide seed treatments and interactions between common tank-mixed foliar herbicides and selected foliar insecticides. No interactions in thrips control or plant growth were observed when …

Nitrogen Management Practices And Colored Plastic Mulch Films Affecting Spider Mites And Aphids On Winter Strawberry In A High Tunnel, Kevin Philip Durden

Graduate Theses and Dissertations

Off season high tunnel strawberry production has the potential to augment income for Arkansas fruit growers during an unproductive time of the year, however management guidelines do not exist. At the University of Arkansas Agricultural Research and Extension Center in Fayetteville, AR studies were conducted to evaluate the effects of four nitrogen (46-0-0 urea fertilizer) fertigation rates (0.75, 0.50, 0.25, 0.0 kg N/day/ha) and red or black colored plastic mulch films on densities of twospotted spider mite, Tetranychus urticae Koch (Acari: Tetranychidae), cotton aphid, Aphis gossypii Glover (Hemiptera: Aphididae), numbers of parasitized cotton aphids, and fruit yield weight. Foliar and …