Entomology Commons^™

Integrating Genomic And Phenomic Breeding Tools For Wheat Stem Sawfly Resistance In Hard Winter Wheat, Sydney E. Graham

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

The wheat stem sawfly (WSS; Cephus cinctus) is an insect pest that affects wheat (Triticum aestivum) in the Northern Great Plains. This pest causes stem cutting that makes the plants susceptible to lodging and results in up to 30% yield loss. The primary management strategy for producers is utilizing host plant resistance, for which the main trait is stem solidness. This trait increases the pith thickness and provides resistance to the WSS by increasing larval mortality and reducing stem cutting. While stem solidness is largely controlled by a known major gene (SSt1), the phenotypic expression is also …

Maggot Mass Effect On The Development And Survival Of Forensic Blow Flies, Akomavo Fabrice Gbenonsi

School of Natural Resources: Dissertations, Theses, and Student Research

Forensic entomology scientists use blow flies' developing patterns to estimate the postmortem interval (PMI). However, larval aggregations produce metabolic heat, resulting in complex microclimates that may change growth timetables. This study investigates how larval density and associated temperature changes affect the development and survival of two forensically essential blow fly species, Lucilia sericata and Calliphora vicina.

This study examined how larval density and associated thermal profiles affect the development of both blow fly species. Using a split-plot design, we tested four larval densities at 25°C and 30°C, with temperature gradients measured via thermocouple at four mass positions three times …

The Prevalence Of Cytauxzoon Felis In Southern United States, Anna Haynes

Biological Sciences Undergraduate Honors Theses

Cytauxzoonosis, caused by transmission from a tick infected with the pathogen Cytauxzoon felis, is considered an emerging tick-borne disease in domestic felines. While not as fatal in other vertebrates such as the reservoir host Lynx rufus (bobcat), in domestic cats, infection is fatal nearly 100% of the time. This study sought to screen ticks pulled from felines for the pathogen across three states in an area identified as a hotspot. Out of 80 ticks screened, 12 tested positive for the pathogen. The positive population of ticks came from 3 neighboring counties in Southern Missouri: Jasper, McDonald, and Newton. McDonald …

Impact Of Temperature And Microbial Infection On The Honey Bee Hemocytes, Michael E. Oeth

Master's Theses

Introduction

Honey bees (Apis mellifera) rely on hemocytes for immunity, wound healing, and hemolymph homeostasis. While 32°C reflects optimal hive conditions, suboptimal temperatures (22°C) and stressors like pathogens (Varroa destructor, bacteria) or pesticides impair hemocyte function. Using clodronate liposomes (CLD) to deplete hemocytes, we assessed how immune suppression interacts with these stressors.

Methods

Newly emerged (5-day-old) and older (15-day-old) bees were assigned to: CLD, control liposomes (LP), PBS, injection wound, or no treatment. Cohorts were exposed to 32°C (optimal) or 22°C (stressful for non-immunosuppressed bees). Fifteen-day-old bees were challenged with Escherichia coli or Staphylococcus aureus. Survival, hemolymph volume, and hemocyte …

Illustrating Insects: Using Art To Encourage Experiences In Entomology, Nia Kaufmann

Honors Program: Senior Projects (Public)

The goal of this creative project is to use art to highlight the diversity of our insect neighbors and encourage others to explore their local environments. In order to accomplish this, I created a poster-sized illustration of five insects I’ve encountered on the University of Nebraska-Lincoln’s City Campus. All insects are posted within the silhouette of Memorial Stadium, each with an ecological and personal significance. The contrast of a well-known building with less popular insects invokes the audience to reconsider how much attention they pay to the wilderness around them.

Keywords: Entomology, Natural Biodiversity, Illustration, Insects, Appreciation of Nature

Landscape Effects On The Beneficial Arthropod Community In Agriculturally Dominated Environments, Hannah E. Stowe

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Ecosystem services in agriculturally dominated regions rely, at least in part, on arthropods. Environment and biodiversity influence beneficial arthropods in population size, community structure, diversity, and ecosystem service provisioning. Predation is among the most important services provided by arthropods in agroecosystems. Understanding what alters predation and predator activity can support the successful application of biological control and, more broadly, integrated pest management. Land use and edge adjacency, field characteristics, landscape, seasonality, and diel period all alter predatory behavior. Crop fields dominate Nebraska agroecosystems, although interspersed perennial grasslands persist through the Conservation Reserve Program (CRP). Such grasslands supply crucial resources and …

Ndp 54 V1 - National Diagnostic Protocol For Meadow Spittlebug (Philaenus Spumarius), Melinda Moir

Biosecurity research reports

National Diagnostic Protocols (NDPs) are diagnostic protocols for the unambiguous taxonomic identification of plant pests. NDPs:

• are a verified information resource for plant health diagnosticians
• are consistent with ISPM No. 27 – Diagnostic Protocols for Regulated Pests
• provide a nationally consistent approach to the identification of plant pests enabling transparency when comparing diagnostic results between laboratories; and,
• are endorsed by regulatory jurisdictions for use (either within their own facilities or when commissioning from others) in a pest incursion.

Where an International Plant Protection Convention (IPPC) diagnostic protocol exists it should be used in preference to NDPs, unless it is shown …

Do Behavioral Attitudes Of Livestock Farmers In Cameroon Influence The Adoption Of Insect-Based Feed? An Application Of The Theory Of Planned Behavior, Ruth T. Nembu, Boris D. Soh Wenda, Hervé K. Mube, Hervé W. Napi, Shaphan Y. Chia, Sali A. Ndindeng, Rodrigue B. Kamga, Dorothy E. Fon

All Peer-Reviewed Publications

This study investigates the effects of behavioral factors on the adoption of insect-based feed technology among livestock farmers in Cameroon. We use partial least squares structural equation modeling (PLS-SEM) to analyze primary data collected from 131 poultry and fish farmers in the Centre region of Cameroon. Our findings reveal that 18.32 % of the surveyed farmers are users of insect-based feed, whereas 81.68 % are non-users. We also show that livestock farmers’ attitudes directly influence the decision to use insect-based feed. Our findings suggest that perceived behavior control and subjective norms indirectly reinforce the decision to use insect-based feed-through attitudes. …

Thermal Performance Drifts Between The Egg Parasitoid Telenomus Remus And The Fall Armyworm May Threaten The Efficacy Of Biological Control Under Climate Change, Macdonald Mubayiwa, Honest Machekano, Brighton M. Mvumi, Winnifred A. Opio, Bame Segaiso, Frank Chidawanyika, Casper Nyamukondiwa

All Peer-Reviewed Publications

The fall armyworm, Spodoptera frugiperda J.E. Smith, is a significant global agricultural pest known for its rapid invasion and devastating impact on crops. While pesticides may be effective for controlling the pest in the short-term, they cause several socioeconomic and ecological costs that highlight the need for more sustainable management strategies. Telenomus remus (Nixon) is a promising egg parasitoid for its biological control. For the parasitoid to provide effective ecosystem services, it should be able to survive and coexist within the same ecological niches as its host. However, there is limited information regarding the potential responses of T. remus to …

Stomoxys Flies (Diptera, Muscidae) Are Competent Vectors Of Trypanosoma Evansi, Trypanosoma Vivax, And Other Livestock Hemopathogens, Julia W. Muita, Joel L. Bargul, John Mark O. Makwatta, Ernest M. Ngatia, Simon K. Tawich, Daniel K. Masiga, Merid N. Getahun

All Peer-Reviewed Publications

Stomoxys flies are widely distributed and economically significant vectors of various livestock pathogens of veterinary importance. However, the role of Stomoxys spp. in pathogen transmission is poorly understood. Therefore, we studied the feeding patterns of these blood feeders collected from specific locations in Kenya to identify various vertebrate hosts they fed on, the livestock hemopathogens they carried, and to elucidate their role in pathogens transmission. Our findings show that field-collected Stomoxys flies carried several pathogens, including Trypanosoma spp., Anaplasma spp., and Theileria spp., which were also detected in the blood of sampled livestock, namely camels and cattle. The findings on …

Honey Bee Colony Losses And Causes During The Active Beekeeping Season 2022/2023 In Nine Sub-Saharan African Countries, Beatrice T. Nganso, Workneh Ayalew, Abebe J. Wubie, Freweini Assefa, Lulseged Belayhun, Nelly N. Ndungu, Daniel Toroitich, Z. Ngalo Otieno-Ayayo, Mbatha B. Wambua, Yudah O. Oyieyo, Ntirenganya Elie, Rachidatou Sikirou, Souradji B. Idrissou, Willy Mwiza, S. Turner, Bridget O. Bobadoye, Sidonie T. Fameni, Sayemie Gaboe, Mawufe K. Agbodzavu, Patrick Mafwila, Geraud C. Tasse Taboue, Kimathi Emily, Tonnang Z.E. Henri, Saliou Niassy, Simplice N. Fonkou, Christian W.W. Pirk, Alison Gray, Robert Brodschneider, Victoria Soroker, Sevgan Subramanian

All Peer-Reviewed Publications

This study reports for the first-time a multi-country survey of managed honey bee colony loss rates and associated risk factors during the active beekeeping season 2022/2023 in nine Sub-Saharan African countries, namely Kenya, Ethiopia, Rwanda, Uganda, Benin, Liberia, Nigeria, Cameroon and Democratic Republic of the Congo. It also evaluates the sustainability of bee swarm catches as a primary source for expanding apiary size by African beekeepers. In this survey, the 1,786 interviewed beekeepers across these countries collectively managing 41,761 colonies registered an overall loss rate of 21.3%, which varied significantly among countries (from 9.7 to 45.3%) and hive types (from …

Influence Of Colony And Hive Attributes, Landscape, And Seasonality On Occurrence Of Large Hive Beetles (Coleoptera: Scarabaeidae) In Taita Taveta County, Kenya, Mbatha B. Wambua, Ayuka T. Fombong, Kiatoko Nkoba, Gladys K. Onyambu, Helen Lydiah Kutima, Sevgan Subramanian, Beatrice T. Nganso

All Peer-Reviewed Publications

Recently, an unexpectedly large number of large hive beetles, Oplostomus haroldi (Witte), was observed in Taita Taveta County (TTC), Kenya, suggesting a potential threat to the apicultural industry. This study investigated the influence of colony and hive attributes, landscape characteristics, and seasonality on the occurrence of O. haroldi within beehives in TTC. Field surveys assessed beetle populations alongside colony strength parameters, environmental variables, and hive types using standard protocols. Our findings revealed significant positive correlations between beetle occurrence and colony phenotypes, such as capped brood, pollen, and honey quantities. Higher beetle populations were also observed in areas with tree cover …

Constructing An Attractive Targeted Sugar Bait For Reducing Mosquito Populations, Xi Xian Ng

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Mosquito-borne pathogen transmission continues to pose a significant burden to global public health, well-being, and economic productivity. In the absence of universally available vaccines and amidst rising insecticide resistance that leads to product failures, there is an urgent need to develop new tools to mitigate mosquito-borne disease risk. Two general strategies exist to reduce transmission risk: personal protective measures and area-wide chemical interventions. Attractive Targeted Sugar Bait (ATSB) systems leverage the sugar-feeding behavior of mosquitoes—critical for sustaining their flight, metabolism, development, and fecundity—by delivering oral toxicants through attractively formulated sugar baits. This study aimed to develop an optimized ATSB formulation …

Efficiency Versus Quality: A Reflection On Making A Children’S Book, Elizabeth Diehl

Honors Projects

When first looking at a children’s book, it appears to be a simple creative project. However, when one finds out the components that go into making one, it becomes clear that simplicity is deceiving. Research, ideation, rough drafts, and scripts are just a few of the necessary components for the finished product, and each one of these components can take multiple days or weeks to complete. This essay is a reflection on my experience of creating a picture book in one semester as I attempt to answer the questions “What if the most efficient process for creating a children's picture …

Dietary Preferences And Food-Seeking Behavior Of Discoid Cockroaches, Blaberus Discoidalis, Claire O'Brien, Darcci Freie, Robert Larson

2025 Student Academic Showcase

Blaberus discoidalis, commonly known as discoid cockroaches, are native to South American tropics where they consume leaf litter and decaying organic matter on forest floors. This experiment expands upon previous research concerning the diet preference of discoid cockroaches based on their loitering time when presented with different food types. Utilizing their preferred foods, peanuts, leaf litter, and banana, this experiment aims to determine how much of each food is being consumed to further support their diet preferences.

Meta-Transcriptomes Of The Microbial Communities Of The Different Developmental Life Stages Of Anastrepha Ludens (Diptera: Tephritidae), Erin L. Schuenzel

School of Integrative Biological & Chemical Sciences Faculty Publications

The Mexican fruit fly, Anastrepha ludens, is a polyphagous pest that is a constant threat to US agriculture because of its proximity to citrus production in border region of the United States and Mexico. Sterile Insect Technique has been used to manage and eradicate of the Mexican Fruit Fly. SIT, however, requires millions of sterile male flies to be released each week from mass-rearing facilities. The mass-reared colonies must deal with microbial infections that reduce survivorship, fecundity and male-male competition. Meta-transcriptomics joins culturing and next generation DNA sequencing techniques to understand these changing microbial communities. This study uses advances …

Contribution To The Knowledge Of Cave-Dwelling Fauna From The Prokletije Mountains, Joanna Kocot-Zalewska, Iwona Słowińska, Paweł Domagała, Jakub Bienias, Dominik Szymański, Dawid Szymański, Hubert Szymański, Paweł Sienkiewicz, Andrzej Józef Woźnica

International Journal of Speleology

The article presents the results of the study on invertebrate fauna inhabiting caves in the Prokletije Mountains. This mountain range is located on the border of Montenegro, Albania, and Kosovo. During the expeditions to Albania in 2021 and to Montenegro in 2022–2023, 17 caves and one bauxite mine were visited altogether. All the studied caves are located very high, above 1,700 m a.s.l., where the temperature inside is low, and several are filled with ice or snow. During the Albanian expedition 102 arthropods have been collected altogether. Among these were 19 beetles (Coleoptera), 44 flies (Diptera), three crickets (Orthoptera), five …

Mapping Mosquito Habitats With Girl Scouts: A Citizen Science Approach Using The “Mosquito Mission: Scout & Report!” Worksheet, Claire A. Klein

Honors Program: Senior Projects (Public)

Mosquitoes, though small, impact millions around the world by being significant vectors of diseases. The search for effective mosquito control efforts is ongoing, however, there is a gap in community involvement with a focus on recognizing and reducing potential breeding areas. Public engagement is a cornerstone of effective mosquito control. This honors thesis investigates the implementation of the Mosquito Mappers program with an original Girl Scout worksheet, “Mosquito Mission: Scout & Report!” This worksheet, which utilizes NASA’s GLOBE Observer Mosquito Habitat Mapper app, enables the Girl Scouts to contribute to real-time environmental monitoring. By guiding Girl Scouts through an inquiry-based …

Unearthing Connections: Characterizing Populations Of Local Harvester Ants, Carson Lyman

Student Research Symposium

What is Hybridization?

• Hybridization is the combination of two different species or subspecies, to create hybrid offspring¹.
• The offspring inherits some genetic material from both parents and so exhibits characteristics of both.

Exploring The Role Of Ethanol In Bumblebee-Yeast Interactions, Austen Grizzell

Student Research Symposium

Why care? Bumblebees are known to be attracted to yeasts present in nectar when foraging. Yeasts produce ethanol via fermentation of sugars found in flower nectar and bee honey. Ethanol may act as a foraging cue for bumblebee species. This has wider implications for plant-pollinator interactions, including the pollination of dozens of bumblebee-pollinated crops such as tomato, eggplant, and strawberries.