Biofouling Community Structure In A Tropical Estuary Of Goa On The West Coast Of India,
2018
Universiti Brunei Darussalam
Biofouling Community Structure In A Tropical Estuary Of Goa On The West Coast Of India, Desai Dattesh V, Krishnamurthy Venkat, Anil Arga Chandrashekar
ASEAN Journal on Science and Technology for Development
Biofouling community structure was studied in a tropical monsoon-influenced Mandovi estuary in Goa, west coast of India. Monthly, seasonal and yearly observations on biofouling on polyvinyl chloride (PVC) panels immersed at subsurface water level were recorded and photographed from May 2012 to September 2013. The barnacle Balanus amphitrite was the dominant fouling organism followed by calcareous polychaetes. The settlement and recruitment of barnacles took place year-round, with the exception of July 2012 and June 2013 (monsoon months). However, their peak abundance was observed during the later months of monsoon (August and September). Polychaetes were dominant during late post-monsoon and pre-monsoon …
Ballast Water Risk Assessment: The Indian Experience,
2018
Universiti Brunei Darussalam
Ballast Water Risk Assessment: The Indian Experience, Krishnamrthy Venkat, Sawant Subhash, Chandrashekar Anil Arga
ASEAN Journal on Science and Technology for Development
The Ballast Water Risk Assessment serves as a “Decision Support System” for any given port to take appropriate ballast water management actions. Risk assessment involves collation of data from the ballast water reporting forms to identify the source of ballast water. The environmental characteristics of the source and recipient ports are utilized to evaluate the similarities. This is then combined with other risk factors, including voyage duration and risk species to gain a preliminary indication of the overall risk posed by each source port. The results will help in evaluating the risk posed by ballast water introductions, and decide whether …
Application Of Fluorescence In Situ Hybridization-Flow Cytometry (Fish-Fcm) Technique To Detect And Quantify Vibrio Cholerae Population From Different Geographic Regions,
2018
Universiti Brunei Darussalam
Application Of Fluorescence In Situ Hybridization-Flow Cytometry (Fish-Fcm) Technique To Detect And Quantify Vibrio Cholerae Population From Different Geographic Regions, Khandeparker Lidita, Desai Dattesh V., Anil Arga Chandrashekar, Sawant S. S., Venkat K., Mapari Kaushal, Jolkifli Zuliza, Abd. Karim, Thoha Hikmah, Hadiyanto Hadiyanto, Dalasane Soukaseum, Chounlamountry Kongngeun, Khaing Myint Myint, Dungca Jenelle Clarisse, Azanza Rhodora, Lim Chin Sing, Tan Koh Siang, Kajonwattanakul Sumana, Phuttapreecha Ratchanee, Le Hoang Mai
ASEAN Journal on Science and Technology for Development
Rapid and species-specific detection, and quantification of pathogenic bacteria are fundamental for monitoring and assessment of the risk they pose to any ecosystem. The study employed Vibrio cholerae, a human pathogen responsible for the life-threatening diarrhoeal disease, cholera and one among the most unwanted from marine bioinvasion point of view. The present study coupled fluorescence in situ hybridization (FISH) technique, a powerful tool in molecular phylogenetic discrimination, with flow cytometry (FCM), a technique used for rapid and accurate quantification of both viable but non-cultivable and non-viable microorganisms. The FISH-FCM technique was used for the first time to quantify V. cholerae …
Role Of Reporting In Compliance Monitoring And Enforcement Of Ballast Water Management,
2018
Universiti Brunei Darussalam
Role Of Reporting In Compliance Monitoring And Enforcement Of Ballast Water Management, Mapari Kaushal E., Krishnamurty Venkat, Anil Arga Chandrashekar
ASEAN Journal on Science and Technology for Development
The Decision Support System (DSS) for ballast water management in any given port is dependent on the availability of information on ballast water carried by a ship in advance. Collation of information through Ballast Water Reporting Forms (BWRF) has been adopted by several countries. This paper provides a comparison of the reporting forms adopted by some of the countries and the International Maritime Organization (IMO) recommended BWRF. The manually submitted reporting forms have several limitations and India has developed a self-validating Electronic Ballast Water Reporting Form (e-BWRF) to overcome such issues. In addition, the possible direction for reporting in the …
Fouling Polychaetes In Tanjung Priok Port Of Jakarta, Indonesia,
2018
Universiti Brunei Darussalam
Fouling Polychaetes In Tanjung Priok Port Of Jakarta, Indonesia, Hadiyanto Hadiyanto
ASEAN Journal on Science and Technology for Development
Fouling polychaetes in Tanjung Priok Port, Jakarta and their status were studied in order to update the list of marine alien species in Indonesia. Polychaetes were collected using six PVC panels that were submerged at a depth of 0.5 m over three months in Jakarta Bay. Polychaetes were identified to the lowest taxon possible based on characteristics of external morphology. The geographical distribution of identified polychaetes was then determined based on taxonomic literature in order to justify whether they were native or alien species for Indonesian waters. Twelve fouling polychaetes belonging to nine families are reported here. Of these, nine …
Challenges In Managing Marine Bio-Invasions Via Shipping In Singapore,
2018
Universiti Brunei Darussalam
Challenges In Managing Marine Bio-Invasions Via Shipping In Singapore, Sing Lim Chin, Tan Koh Siang
ASEAN Journal on Science and Technology for Development
The economic, social and environmental impacts arising from the transfer and establishment of non indigenous marine species (NIMS) mediated through ship hull biofouling and ballast water discharges in the coastal marine environment require a regional approach to manage bio-invasions. As the coordinating body for maritime shipping affairs and protection of the marine environment, the International Maritime Organization (IMO) has been working with member states to adopt and ratify global conventions aimed at preventing invasive species transfer through shipping. As a major shipping hub and port-of-call in Southeast Asia, Singapore faces multiple challenges in managing marine bio-invasions including managing the transit …
Numerical Modelling Of Ballast Water Dispersion In Different Bioregions Along The Coast Of India,
2018
Universiti Brunei Darussalam
Numerical Modelling Of Ballast Water Dispersion In Different Bioregions Along The Coast Of India, Babu M.T., Sudheesh K, Vethamony P, Anuvindha S
ASEAN Journal on Science and Technology for Development
Aquatic organisms and pathogens may become major threats to the coastal and marine environment when introduced into a region beyond their natural distributions through ballast water (BW). Coastal currents induced by tides and winds, especially ebb currents, may facilitate the spread of these marine organisms along nearshore and inshore areas. Numerical modelling of hydrodynamics is an effective tool to track the dispersion of these organisms in the receiving water body through BW release. Particle transport models can be used to track the advection and dispersion of these organisms. Alternatively, the difference in salinity of the BW and coastal waters can …
Recent Trends Of Demersal Marine Fish And Invertebrate Production In Southeast Asia – A Hypothesis-Based Analysis,
2018
Universiti Brunei Darussalam
Recent Trends Of Demersal Marine Fish And Invertebrate Production In Southeast Asia – A Hypothesis-Based Analysis, Padate Vinay P., Rivonker Chandrashekher U.
ASEAN Journal on Science and Technology for Development
Demersal marine fish and invertebrate production data for Southeast Asia (1996–2007) obtained from the Southeast Asian Fisheries Development Center (SEAFDEC) statistical bulletin indicated a reduced production by Thailand largely due to over-exploitation and altered coastal ecosystems. In contrast, increased production by Philippines, Indonesia and Malaysia was due to an increase in mechanised fishing fleets. Moreover, marginal increases in the ecosystem indicators were attributed to increased exploitation of high and mid trophic level organisms suggesting the development of “top-down” cascade effect in the future. In this bioregion, land use pattern affecting water quality coupled with altered monsoonal sequences and rising sea …
Survey Of Fouling Organisms At Songkhla Port In Thailand,
2018
Universiti Brunei Darussalam
Survey Of Fouling Organisms At Songkhla Port In Thailand, Phuttapreecha Ratchanee, Kajonwattanakul Sumana, Songkai Phatcharin, Choamanee Chaovadee
ASEAN Journal on Science and Technology for Development
The fouling organisms at Songkhla Port were investigated from November 2011 to December 2012. Samples were collected using PVC panels (10 cm x 20 cm) submerged for one-month and three-month periods. Analysis of fouling panels was carried out using PhotoGrid software. In addition to slime and silt, three types of fouling organisms, namely encrusting bryozoa, barnacles and calcareous polychaetes, were observed on the submerged PVC panels. Calcareous polychaetes and molluscs were the most diverse groups (ten species) on panels that were submerged for one-month and three-month periods. Barnacles (Balanus spp.) were most abundant on panels submerged for three-month periods, while …
Development Of A Reliable Method To Express And Purify Vaccinia Virus Transcription Factors A7 And D6 In Bacteria,
2018
Seton Hall University
Development Of A Reliable Method To Express And Purify Vaccinia Virus Transcription Factors A7 And D6 In Bacteria, Younghoon Park
Seton Hall University Dissertations and Theses (ETDs)
Poxvirus early genes code for viral products involved in host interaction, immune evasion, replication, and transcription of the viral genome. These viral early genes contain a unique and conserved promoter sequence that is recognized by early transcription factors (ETFs). The working hypothesis is pharmacologically targeting the structure of the poxvirus early promoters can inhibit transcription of poxvirus early genes. Our group has identified unique secondary structure which deviates from a scrambled control near the Vaccinia Virus (VACV) E9L promoter. Targeting these unique structures, which most likely may be G-quadruplexes, with structure specific ligands may distort local DNA structures at the …
Moderate Heat Application Enhances The Efficacy Of Nanosecond Pulse Stimulation For The Treatment Of Squamous Cell Carcinoma,
2018
Old Dominion University
Moderate Heat Application Enhances The Efficacy Of Nanosecond Pulse Stimulation For The Treatment Of Squamous Cell Carcinoma, Chelsea M. Edelblute, Sigi Guo, Embo Yang, Chunqi Jiang, Karl Schoenbach, Richard Heller
Bioelectrics Publications
Nanosecond pulse stimulation as a tumor ablation therapy has been studied for the treatment of various carcinomas in animal models and has shown a significant survival benefit. In the current study, we found that moderate heating at 43°C for 2 minutes significantly enhanced in vitro nanosecond pulse stimulation-induced cell death of KLN205 murine squamous cell carcinoma cells by 2.43-fold at 600 V and by 2.32-fold at 900 V, as evidenced by propidium iodide uptake. Furthermore, the ablation zone in KLN205 cells placed in a 3-dimensional cell-culture model and pulsed at a voltage of 900 V at 43°C was 3 times …
Supercharged Models Of Intrinsically Disordered Proteins And Their Utility In Sensing,
2018
CUNY Graduate Center
Supercharged Models Of Intrinsically Disordered Proteins And Their Utility In Sensing, Peter J. Schnatz
Dissertations, Theses, and Capstone Projects
In this thesis I show that greatly increasing the magnitude of a protein’s net charge using surface supercharging transforms that protein into a ligand-gated or counterion-gated conformational molecular switch. To demonstrate this I first modified the designed helical bundle hemoprotein H4 using simple molecular modeling, creating a highly charged protein which both unfolds reversibly at low ionic strength and undergoes the ligand-induced folding transition commonly observed in signal transduction by intrinsically disordered proteins in biology. Due to the high surface charge density, ligand binding to this protein is allosterically activated by low concentrations of divalent cations and the polyamine spermine. …
Induction Of Oil Accumulation By Heat Stress Is Metabolically Distinct From N Stress In The Green Microalgae Coccomyxa Subellipsoidea C169,
2018
University of Nebraska-Lincoln
Induction Of Oil Accumulation By Heat Stress Is Metabolically Distinct From N Stress In The Green Microalgae Coccomyxa Subellipsoidea C169, James W. Allen, Rahul Tevatia, Yaşar Demirel, Concetta C. Dirusso, Paul N. Black
Department of Biochemistry: Faculty Publications
Algae are often promoted as feedstock organisms to produce a sustainable petroleum fossil fuel alternative. However, to induce lipid accumulation most often requires a severe stress that is difficult to induce in large batch cultures. The objective of this study is to analyze and mathematically model heat stress on growth, chlorophyll content, triacylglyceride, and starch synthesis in algae. We initially screened 30 algal species for the most pronounced induction of lipid droplets from heat stress using confocal microscopy and mass spectroscopy techniques. One species, Coccomyxa subellipsoidea C169, was selected and subjected to further biochemical analyses using a jacketed bioreactor amended …
Microfluidic Electrical Impedance Spectroscopy,
2018
California Polytechnic State University, San Luis Obispo
Microfluidic Electrical Impedance Spectroscopy, John J. Foley
Master's Theses
The goal of this study is to design and manufacture a microfluidic device capable of measuring changes in impedance valuesof microfluidic cell cultures. Tocharacterize this, an interdigitated array of electrodes was patterned over glass, where it was then bonded to a series of fluidic networks created in PDMS via soft lithography. The device measured ethanol impedance initially to show that values remain consistent over time. Impedance values of water and 1% wt. saltwater were compared to show that the device is able to detect changes in impedance, with up to a 60% reduction in electrical impedance in saltwater. Cells were …
Identification Of Biologically Essential Nodes Via Determinative Power In Logical Models Of Cellular Processes,
2018
University of Nebraska at Omaha
Identification Of Biologically Essential Nodes Via Determinative Power In Logical Models Of Cellular Processes, Trevor Pentzien, Bhanwar L. Puniya, Tomas Helikar, Mihaela T. Matache
Department of Biochemistry: Faculty Publications
A variety of biological networks can bemodeled as logical or Boolean networks. However, a simplification of the reality to binary states of the nodes does not ease the difficulty of analyzing the dynamics of large, complex networks, such as signal transduction networks, due to the exponential dependence of the state space on the number of nodes. This paper considers a recently introduced method for finding a fairly small subnetwork, representing a collection of nodes that determine the states of most other nodes with a reasonable level of entropy. The subnetwork contains the most determinative nodes that yield the highest information …
Direct Quantification Of Deubiquitinating Enzyme Activity In Single Intact Cells,
2018
Louisiana State University and Agricultural and Mechanical College
Direct Quantification Of Deubiquitinating Enzyme Activity In Single Intact Cells, Nora Safabakhsh
LSU Doctoral Dissertations
Challenges in drug efficacy occur during the treatment of most types of cancer due to the heterogeneity of the tumor microenvironment. This has led to the development of personalized medicine. Due to the clinical success of the proteasome inhibitors Bortezomib and Carfilzomib in treatment of multiple myeloma, interest has shifted towards molecularly-targeted chemotherapeutics for ubiquitin-proteasome system (UPS). Deubiquitinating enzymes (DUBs) are an essential part of this pathway which have been found to promote Bortezomib resistance in multiple myeloma patients. Unfortunately, there is a lack of specific, high throughput biochemical assays to characterize DUB activity in patient samples before and after …
Targeting Neuropeptides To Bone Fractures For Accelerated Healing,
2018
Purdue University
Targeting Neuropeptides To Bone Fractures For Accelerated Healing, Nicholas A. Young, Jeffery J. Nielsen, Philip S. Low
The Summer Undergraduate Research Fellowship (SURF) Symposium
In patients over the age of 65 especially, bone fractures represent a significant disease burden. Non-invasive drug therapies are not available for bone fractures which represents a problem for this population. Vasoactive intestinal peptide (VIP) and Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP), two neuromodulator peptides in the glucagon superfamily, have demonstrated positive regulation of osteoblast proliferation and activity. Using acidic oligopeptides, we have developed ligands that target to and accumulate at fracture sites. These targeting ligands can be synthesized in sequence with bone anabolic peptides to minimize off target effects and increase potency at the fracture site to create safer and …
Cost-Effective Paper-Based Diagnostic Using Split Proteins To Detect Yeast Infections,
2018
Department of Agricultural and Biological Engineering, Purdue University
Cost-Effective Paper-Based Diagnostic Using Split Proteins To Detect Yeast Infections, Zachary R. Berglund, Kevin V. Solomon, Mohit S. Verma, Moiz Rasheed, Zachary Hartley, Kevin Fitzgerald, Kok Zhi Lee, Janice Chan, Julianne Dejoie, Makayla Schacht, Alex Zavala
The Summer Undergraduate Research Fellowship (SURF) Symposium
The common yeast infection, vulvovaginal candidiasis, affects three out of four women throughout their lifetime and can be spread to their child in the form of oral candidiasis (thrush). This disease is caused by the fungal pathogen Candida albicans, which is also a major cause of systemic candidiasis, a rarer but deadly disease with up to a 49% lethality rate. Current widely-used diagnostic methods include cell cultures, pH tests, and antibody detection, to assist effective treatment. Despite availability of various diagnostic methods, there is no inexpensive, rapid, and accurate way to detect C. albicans infection. This project aims to …
Engineering Bioluminescent Sensors Of Cyclic Amp To Study Opioid Signaling,
2018
Purdue University
Engineering Bioluminescent Sensors Of Cyclic Amp To Study Opioid Signaling, Alexander L. Tesmer, Alexander R. French, Mathew Tantama
The Summer Undergraduate Research Fellowship (SURF) Symposium
Opioids are small signaling molecules which bind to opioid receptors on the surface of cells. The kappa opioid receptor (KOR) is one of three major types of opioid receptors found in human neurons. When an opioid binds to a KOR, a variety of biochemical signaling pathways are activated inside the cell. Each of these pathways are associated with different physiological effects of KOR activation. The production of a small signaling molecule, cyclic adenosine monophosphate (cAMP), is known to be inhibited during KOR activation of the analgesic (pain-killing) signaling pathway. The ability to interrogate the individual responses of KOR signaling pathways …
Genome Analysis Of Multiple Mycobacteriophage,
2018
Purdue University
Genome Analysis Of Multiple Mycobacteriophage, Emily Kerstiens, Kari Clase, Yi Li, Gillian Smith, Sarah Bell
The Summer Undergraduate Research Fellowship (SURF) Symposium
Bacteriophage are viruses that infect and kill bacteria. They can be used as treatments for antibiotic resistant bacterial infections, but more knowledge is needed about phage and how they interact with bacteria in order to develop safe and effective phage therapy treatments. This study examines the genomes of eighteen mycobacteriophage that were isolated from the environment on and surrounding Purdue University. Phage genomes were annotated using several bioinformatics software, including DNA Master, GeneMark, and PECAAN. Evidence was examined to determine the correct location within the genome and the potential function. Approximately two thousand genes were annotated in this study. A …
