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Composition Of The Adult Digestive Tract Bacterial Microbiome Based On Seven Mouth Surfaces, Tonsils, Throat And Stool Samples, Nicola Segata, Susan Kinder Haake, Peter Mannon, Katherine P. Lemon, Levi Waldron, Dirk Gevers, Curtis Huttenhower, Jacques Izard 2012 Harvard University

Composition Of The Adult Digestive Tract Bacterial Microbiome Based On Seven Mouth Surfaces, Tonsils, Throat And Stool Samples, Nicola Segata, Susan Kinder Haake, Peter Mannon, Katherine P. Lemon, Levi Waldron, Dirk Gevers, Curtis Huttenhower, Jacques Izard

Department of Food Science and Technology: Faculty Publications

Background: To understand the relationship between our bacterial microbiome and health, it is essential to define the microbiome in the absence of disease. The digestive tract includes diverse habitats and hosts the human body’s greatest bacterial density. We describe the bacterial community composition of ten digestive tract sites from more than 200 normal adults enrolled in the Human Microbiome Project, and metagenomically determined metabolic potentials of four representative sites.

Results: The microbiota of these diverse habitats formed four groups based on similar community compositions: buccal mucosa, keratinized gingiva, hard palate; saliva, tongue, tonsils, throat; sub- and supra-gingival plaques; …


Metabolic Reconstruction For Metagenomic Data And Its Application To The Human Microbiome, Sahar Abubucker, Nicola Segata, Johannes Goll, Alyxandria M. Schubert, Jacques Izard, Brandi L. Cantarel, Beltran Rodriguez-Mueller, Jeremy Zucker, Mathangi Thiagarajan, Bernard Henrissat, Owen White, Scott T. Scott, Barbara Methé, Patrick D. Schloss, Dirk Gever, Makedonka Mitreva, Curtis Huttenhower 2012 Washington University School of Medicine

Metabolic Reconstruction For Metagenomic Data And Its Application To The Human Microbiome, Sahar Abubucker, Nicola Segata, Johannes Goll, Alyxandria M. Schubert, Jacques Izard, Brandi L. Cantarel, Beltran Rodriguez-Mueller, Jeremy Zucker, Mathangi Thiagarajan, Bernard Henrissat, Owen White, Scott T. Scott, Barbara Methé, Patrick D. Schloss, Dirk Gever, Makedonka Mitreva, Curtis Huttenhower

Department of Food Science and Technology: Faculty Publications

Microbial communities carry out the majority of the biochemical activity on the planet, and they play integral roles in processes including metabolism and immune homeostasis in the human microbiome. Shotgun sequencing of such communities’ metagenomes provides information complementary to organismal abundances from taxonomic markers, but the resulting data typically comprise short reads from hundreds of different organisms and are at best challenging to assemble comparably to single-organism genomes. Here, we describe an alternative approach to infer the functional and metabolic potential of a microbial community metagenome. We determined the gene families and pathways present or absent within a community, as …


Aquatic Heteroptera Of The Lake Manguao Catchment, Palawan And New Rank Of Rhagovelia Kawakamii Hoberlandti Hungerford & Matsuda 1961, Hendrik Freitag, Herbert Zettel 2012 Ateneo de Manila University

Aquatic Heteroptera Of The Lake Manguao Catchment, Palawan And New Rank Of Rhagovelia Kawakamii Hoberlandti Hungerford & Matsuda 1961, Hendrik Freitag, Herbert Zettel

Biology Faculty Publications

Results of an inventory of the fauna of aquatic and semiaquatic true bugs (Insecta: Hemiptera) of small streams in a lake catchment of northern Palawan are presented. Twenty-one species were recorded. Taxonomic and ecological notes, distribution and collection sites are given for each identified taxon. Rhagovelia hoberlandti Hungerford & Matsuda 1961 is newly ranked as a subspecies of R. kawakamii (Matsumura 1913): Rhagovelia kawakamii hoberlandti Hungerford & Matsuda 1961, stat.n. Cercotmetus asiaticus Amyot & Serville 1843 is recorded for the first time from the Philippines. Endemic and few undescribed taxa are discussed. Additional environmental data of the sampled waters are …


Mapping Plant Biodiversity Hotspots At The County Scale: A New Tool For Establishing Resource Conservation Strategies, Kristie Haydu 2012 California Polytechnic State University, San Luis Obispo

Mapping Plant Biodiversity Hotspots At The County Scale: A New Tool For Establishing Resource Conservation Strategies, Kristie Haydu

Master's Theses

Myers first identified the world’s 25 biodiversity hotspots and pioneered innovative ideas about the usefulness of biodiversity models for establishing long-term resource conservation strategies at global scales. Since Myers, most of the subsequent studies using hotspot science for biodiversity modeling have used large spatial scales like countries, provinces or states, and other biogeoraphic regions. The California Floristic Province continues to be one of the recognized global biodiversity hotspots. Our study site, San Luis Obispo County is within this hotspot and we created a map of plant biodiversity hotspots at the county scale using GIS technology. We wanted to determine the …


Preserving Wildlife In San Luis Obispo City By Way Of San Luis Obispo Creek, Sydni Nicolici 2012 California Polytechnic State University - San Luis Obispo

Preserving Wildlife In San Luis Obispo City By Way Of San Luis Obispo Creek, Sydni Nicolici

Liberal Arts and Engineering Studies

The effectiveness of San Luis Obispo wildlife easements and city open space utilized to preserve species of concern is correlated to the health and maintenance of the San Luis Obispo Creek. I propose that the health of the Creek be addressed before more open space is added to the green belt surrounding the city. This bottom-up approach to preservation will ensure all steps of habitat will function as they should.


Notes: Red-Tailed Hawk Predation Of A Striped Skunk, Steven G. Platt, Thomas R. Rainwater 2012 Sul Ross State University, Alpine

Notes: Red-Tailed Hawk Predation Of A Striped Skunk, Steven G. Platt, Thomas R. Rainwater

The Prairie Naturalist

Skunks (Mephitidae) are capable of projecting pungent, oily musk from paired anal glands (Verts 1967), which acts as a central nervous system depressant (Wade- Smith and Verts 1982), and can incapacitate birds of prey if directed into the eyes (Garcelon 1981). Consequently, few raptors are known to prey on striped skunks (Mephitis mephitis). Great horned owls (Bubo virginianus) are the only raptor that regularly preys on skunks (Bent 1938b, Lowery 1974, Houston et al. 1998), although occasional instances of predation by golden eagles (Aquila chrysaetos; Olendorff 1976, Palmer 1988a), bald eagles (Haliaeetus leucocephalis; Broley 1952, Wade-Smith and Verts 1982), northern …


Least And Merriam’S Shrews From Banner County, Nebraska, Jennifer N. Merlino, Alexandra R. Frohberg, Jamie Harmon, Keith Geluso 2012 University of Nebraska—Kearney

Least And Merriam’S Shrews From Banner County, Nebraska, Jennifer N. Merlino, Alexandra R. Frohberg, Jamie Harmon, Keith Geluso

The Prairie Naturalist

Four species of shrews occur in the panhandle of western Nebraska – the masked shrew (Sorex cinereus), Merriam’s shrew (S. merriami), dwarf shrew (S. nanus), and least shrew (Cryptotis parva; Genoways et al. 2008). Little information is known regarding the distribution and habitat of those species due to few captures of individuals throughout the region (e.g., Jones 1964, Freeman et al. 1993, Benedict et al. 1999, 2000, Geluso et al. 2004). To date, no shrew has been reported from the southwestern part of the panhandle in Banner, Kimball, or Cheyenne counties. Here we present distributional records for the least shrew …


Grass And Canada Goldenrod (Solidago Canadensis) Competition And Implications For Management In The Northern Tallgrass Prairie, Alexander J. Smart, Gary E. Larson, Peter J. Bauman 2012 South Dakota State University

Grass And Canada Goldenrod (Solidago Canadensis) Competition And Implications For Management In The Northern Tallgrass Prairie, Alexander J. Smart, Gary E. Larson, Peter J. Bauman

The Prairie Naturalist

Canada goldenrod (Solidago canadensis L.) is a native perennial forb that can form dense clonal patches and become weedy in pastures of the Northern Tallgrass Prairie Ecoregion. Our objectives were to determine 1) the competitive effects between Canada goldenrod and grass, 2) the relationship between Canada goldenrod stem density and grass biomass, and 3) the distribution of Canada goldenrod stem density at the pasture scale. We used regression analysis to develop a relationship between Canada goldenrod stem density and grass biomass. Additionally, we estimated the frequency distribution of Canada goldenrod stem density categories using three evenly distributed 100 × 100-m …


Spatiotemporal Variation In Vegetation Structure Resulting From Pyric-Herbivory, Sherry Leis, Lloyd W. Morrison, Michael D. Debacker 2012 Missouri State University

Spatiotemporal Variation In Vegetation Structure Resulting From Pyric-Herbivory, Sherry Leis, Lloyd W. Morrison, Michael D. Debacker

The Prairie Naturalist

Pyric-herbivory is a process that is widely assumed to create greater habitat heterogeneity in grasslands at the landscape scale than could be achieved by either fire or grazing alone. Yet, few studies have actually quantified the effects of pyric- herbivory on vegetation structure within layers of the grass canopy. Here we quantify the effects of pyric-herbivory on a pasture at Tallgrass Prairie National Preserve in Kansas. We subdivided the pasture into three patches and burned one patch each year in a three-year rotation. We estimated visual obstruction for 25-cm strata and recorded maximum vegetation height. We found that recently burned …


Sickleweed On The Fort Pierre National Grassland: An Emerging Threat, Jack L. Bulter, Stefanie D. Wacker 2012 US Forest Service, Rocky Mountain Research Station, Forest and Grassland Research Laboratory

Sickleweed On The Fort Pierre National Grassland: An Emerging Threat, Jack L. Bulter, Stefanie D. Wacker

The Prairie Naturalist

We report the first detailed field survey of sickleweed (Falcaria vulgaris L.) in the United States. Sickleweed is native to Europe, the Mediterranean, Asia Minor, and Iran and was first reported in the United States in 1922. It is listed by the Nebraska Invasive Species Council as a Category II invasive plant species. In recent years, abundance and distribution of sickle- weed has increased dramatically in and around the Fort Pierre National Grassland (FPNG), South Dakota. Management of such a rapidly expanding population is hampered by a general lack of baseline information on the biology and ecology of sickleweed. We …


Spatial Ecology Of Urban Raccoons In Northeastern Ohio: Implications For Oral Rabies Vaccination, Are R. Berentsen, Mike R. Dunbar, Chadd E. Fitzpatrick, W. David Walter 2012 USDA/APHIS/WS/National Wildlife Research Center

Spatial Ecology Of Urban Raccoons In Northeastern Ohio: Implications For Oral Rabies Vaccination, Are R. Berentsen, Mike R. Dunbar, Chadd E. Fitzpatrick, W. David Walter

The Prairie Naturalist

In 1977, rabies was detected in a raccoon (Procyon lotor) in West Virginia, and since the mid-1980s raccoon variant rabies has spread throughout the eastern United States and moved west as far as the eastern edge of Cleveland, Ohio. The primary tool to combat this spread is the distribution of oral rabies vaccine (ORV) baits. A thorough knowledge of raccoon space use is critical in determining bait placement, particularly in urban areas. We monitored nine raccoons in urban areas of Cleveland, Ohio, calculated home range sizes, monitored raccoon movement with respect to potential movement barriers, and used resource selection functions …


Validation Of Pcr-Based Assays And Laboratory Accreditation For Environmental Detection Of Aquatic Invasive Species, Invasive Species Advisory Committee 2012 University of Nebraska - Lincoln

Validation Of Pcr-Based Assays And Laboratory Accreditation For Environmental Detection Of Aquatic Invasive Species, Invasive Species Advisory Committee

National Invasive Species Council

This white paper provides:

a) Background information on the use, accuracy and reliability of PCR-based assays such as environmentally sampled DNA (eDNA) for early detection of aquatic invasive species (AIS) and;

b) Recommendations for establishing a system for validating assays and accrediting laboratories that report on the presence or absence of AIS.

This white paper was developed by the members of ISAC and discusses the need for developing validation requirements for Polymerase Chain Reaction (PCR) and other DNA-based molecular assays that are increasingly being used to detect AIS. It does not provide a simplified checklist for evaluation of their ability …


Northern Lights: Fourth World Nations And The Geopolitical Dance In The Arctic, Heidi G. Bruce 2012 SIT Graduate Institute

Northern Lights: Fourth World Nations And The Geopolitical Dance In The Arctic, Heidi G. Bruce

Capstone Collection

Over the past decade, the Arctic region has received increased attention from climate scientists, politicians, and transnational corporations. Human-induced climate change is causing glaciers to recede, resulting in new northern sea passages that are highly sought after by businesses and governments alike. Deeply affected by this increased northern exposure are Arctic Fourth World nations – politically and culturally distinct nations encapsulated by states – that have lived in the Arctic for millennia. This paper examines the impacts that expanded northern sea routes are having on Arctic Fourth World nations and the conflict mitigation approaches being used in the region. Research …


Fish Assemblage Dynamics And Red Drum Habitat Selection In Bayou St. John And Associated Urban Waterways Located Within The City Of New Orleans, Louisiana, Patrick W. Smith Mr. 2012 University of New Orleans

Fish Assemblage Dynamics And Red Drum Habitat Selection In Bayou St. John And Associated Urban Waterways Located Within The City Of New Orleans, Louisiana, Patrick W. Smith Mr.

LSU New Orleans Theses and Dissertations

Bayou St. John (BSJ) and City Park Lakes and Lagoons (CPLL) are urban waterways in New Orleans, Louisiana. I studied habitat selection of red drum in BSJ, and fish assemblage change in BSJ and CPLL over 40 years. Temperature was found to be the best predictor of red drum habitat selection in Bayou St. John, while salinity and change in depth also were found to be good predictors for certain sites. Potential prey item abundance did not appear to influence habitat selection. Using data from 1971 – 2010, nearshore habitats in CPLL were affected by Hurricane Katrina, but have sense …


Traversing Swanton Road, 8th Ed., James A. West 2012 California Polytechnic State University, San Luis Obispo

Traversing Swanton Road, 8th Ed., James A. West

Academic, Government & Associated Organizational Research Resources

Situated at the northwest end of Santa Cruz County and occupying circa 30 square miles of sharply contrasted terrain, the Scott Creek Watershed concentrates within its geomorphological boundaries, at least 10-12% of California's flora, both native and introduced. Paradoxically, the use/abuse that the watershed has sustained over the past 140+ years, has not necessarily diminished the biodiversity and perhaps parallels the naturally disruptive but biologically energizing processes (fire, flooding, landslides and erosion), which have also been historically documented for the area for +60 years. This is an early edition of an extensive document by James A. West. Please visit the …


Cholesterol Lowering Effects Of Milk With Added Phytosterols, Laura Kells Cusack 2012 University of Connecticut - Storrs

Cholesterol Lowering Effects Of Milk With Added Phytosterols, Laura Kells Cusack

Master's Theses

Foods incorporating plant sterols (PS) reduce Low-density lipoprotein (LDL) on average approximately 10%. PS with a higher lipid solubility may promote greater reductions. We examined the cholesterol lowering effect of a novel triglyceride recrystallized phystosterol (TRP). Twenty subjects (mean ± SD; age, 56 ± 10 years; BMI, 27 ± 5) with elevated LDL (>100 mg/dL) participated in three 4-week phases; Phase I, 2% milk; Phase II, milk with 2.0 grams (g) free PS; Phase III, milk with 2.0 g TRP. Before and after each phase two fasting blood draws were obtained for determination of serum cholesterol. Between the 2% …


Traversing Swanton Road, 7th Ed., James A. West 2012 California Polytechnic State University, San Luis Obispo

Traversing Swanton Road, 7th Ed., James A. West

Academic, Government & Associated Organizational Research Resources

Situated at the northwest end of Santa Cruz County and occupying circa 30 square miles of sharply contrasted terrain, the Scott Creek Watershed concentrates within its geomorphological boundaries, at least 10-12% of California's flora, both native and introduced. Paradoxically, the use/abuse that the watershed has sustained over the past 140+ years, has not necessarily diminished the biodiversity and perhaps parallels the naturally disruptive but biologically energizing processes (fire, flooding, landslides and erosion), which have also been historically documented for the area for +60 years. This is an early edition of an extensive document by James A. West. Please visit the …


Paleoecological Study Of Unguja: Can Past Environments Be Inferred From Fossilized Corals And Mollusks?, Meredith Burger, Jory Lerback 2012 SIT Study Abroad

Paleoecological Study Of Unguja: Can Past Environments Be Inferred From Fossilized Corals And Mollusks?, Meredith Burger, Jory Lerback

Independent Study Project (ISP) Collection

A study was conducted on Unguja, an island off the eastern coast of Tanzania, to provide an overview of the paleoecology found in the cliff shelves across the island. It is known that Zanzibar was a submerged reef from the Neogene, approximately 23 million years ago (Ma). Two tests were carried out in 16 sites around the island. One test looked at coral fossils, while the other studied fossilized shells. Both tests measured biodiversity, with one specifically studying patterns of ocean currents, and the other studying nutrient levels and deposition rates. Biodiversity tests were inconclusive but coral diversity showed a …


Creation Of A Natural History Information Database With Mobile Device Access For The Pace Campus In Pleasantville, Joshua J. Schwartz, Martina Blackwood 2012 Dyson College of Arts and Sciences, Pace University

Creation Of A Natural History Information Database With Mobile Device Access For The Pace Campus In Pleasantville, Joshua J. Schwartz, Martina Blackwood

Cornerstone 3 Reports : Interdisciplinary Informatics

No abstract provided.


A Conceptual Model To Facilitate Amphibian Conservation In The Northern Great Plains, David M. Mushet, Ned H. Euliss Jr., Craig A. Stockwell 2012 Northern Prairie Wildlife Research Center

A Conceptual Model To Facilitate Amphibian Conservation In The Northern Great Plains, David M. Mushet, Ned H. Euliss Jr., Craig A. Stockwell

Great Plains Research: A Journal of Natural and Social Sciences (through 2013)

As pressures on agricultural landscapes to meet worldwide resource needs increase, amphibian populations face numerous threats including habitat destruction, chemical contaminants, disease outbreaks, wetland sedimentation, and synergistic effects of these perturbations. To facilitate conservation planning, we developed a conceptual model depicting elements critical for amphibian conservation in the northern Great Plains. First, we linked upland, wetland, and landscape features to specific ecological attributes. Ecological attributes included adult survival; reproduction and survival to metamorphosis; and successful dispersal and recolonization. Second, we linked ecosystem drivers, ecosystem stressors, and ecological effects of the region to each ecological attribute. Lastly, we summarized information on …


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