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Evaluation Of Intra-Species Diversity Of Oxalobacter Formigenes Strains Using Pulsed-Field Gel Electrophoresis (Pfge) And Multiplex Pcr, Nivedita Pareek 2019 Eastern Illinois University

Evaluation Of Intra-Species Diversity Of Oxalobacter Formigenes Strains Using Pulsed-Field Gel Electrophoresis (Pfge) And Multiplex Pcr, Nivedita Pareek

Masters Theses

Oxalobacter formigenes is a beneficial, strict anaerobic gut bacterium that plays an important role in the prevention of kidney stone disease with a unique characteristic of using only oxalate as a carbon and energy source. To date, twenty-one strains of O. formigenes have been isolated which are further divided into two groups based on differences in the patterns of cell membrane lipids, cellular proteins, and nucleic acid fragments. The objective of the study was to evaluate intra-species diversity of O. formigenes strains using pulsed-field gel electrophoresis (PFGE) and multiplex PCR.

Nine pure cultures of O. formigenes strains (5 strains group …


An Assessment Of Night Time And Seasonal Electrofishing In The Lower Wabash River, Eric Christopher Hine 2019 Eastern Illinois University

An Assessment Of Night Time And Seasonal Electrofishing In The Lower Wabash River, Eric Christopher Hine

Masters Theses

Large rivers are highly important systems; being exploited both commercially and recreationally. Because of this usage by humans, close monitoring of the ecology of theses rivers is of the utmost importance. The Long-Term Electrofishing project (LTEF) monitors the fish communities of the Illinois, Mississippi, Wabash, and Ohio rivers using day time, pulsed-DC electrofishing during the late Spring through the early Fall each year. Given that previous studies have noted diel and seasonal changes in catch and composition of fish communities, the addition of night time electrofishing may be beneficial to the overarching goals of the LTEF. This study sought to …


Elucidating Patterns Of Bat Species Occupancy Across A Disturbed Landscape In California's Central Valley, Trinity N. Smith 2019 Humboldt State University

Elucidating Patterns Of Bat Species Occupancy Across A Disturbed Landscape In California's Central Valley, Trinity N. Smith

Cal Poly Humboldt theses and projects

California’s Central Valley, one of the most productive agricultural regions in the world, is home to 14 species of resident and migratory bats. The Central Valley has been identified as a crisis ecoregion, and a high number of species are at risk due to anthropogenic land use. In addition, the Central Valley has faced severe drought, effects of which are intensified on the natural landscape by agricultural irrigation practices. In response to the historical drought of 2012-2015, California Department of Fish and Wildlife (CDFW) implemented the Terrestrial Species Stressor Monitoring (TSSM) project, which in part aimed to collect information on …


Next-Generation Sequencing, Morphology, And Culture-Based Methods Reveal Diverse Algal Assemblages Throughout The Florida Springs, Alyssa Garvey 2019 University of North Florida

Next-Generation Sequencing, Morphology, And Culture-Based Methods Reveal Diverse Algal Assemblages Throughout The Florida Springs, Alyssa Garvey

UNF Graduate Theses and Dissertations

Algae are a group of highly diverse photosynthetic organisms found in variety of habitats. As the primary energy base in ecosystems, knowledge of the diversity and presence of certain algal lineages is paramount to our understanding of the trophic state of aquatic habitats. In recent years, the state of Florida has seen an increase of both marine and freshwater algal blooms. Similarly, filamentous algae have begun outcompeting vascular macrophytes throughout many of Florida’s springs as nutrient enrichment from anthropogenic sources increases. Traditionally, the Florida algal spring communities have been assessed using classic morphological methods, which may underrepresent the true biodiversity …


Natural History Of The Eastern Harvest Mouse In Southeastern Virginia, Robert K. Rose 2019 Old Dominion University

Natural History Of The Eastern Harvest Mouse In Southeastern Virginia, Robert K. Rose

Biological Sciences Faculty Publications

The Eastern Harvest Mouse, Reithrodontomys humulis, has been studied extensively in southeastern Virginia since 1979, using a combination of live and pitfall trapping methods. This smallest rodent of eastern North America also is one of most versatile, occupying a range of habitats in southeastern Virginia from old fields in different stages of succession, brushy edges, and forests of different types. As with other species of Reithrodontomys, R. humulis often is associated with the Hispid Cotton Rat, Sigmodon hispidus, with both reaching modest densities in old fields. Two capture-mark-release studies of small mammal communities in southern Chesapeake lasting …


Analysis Of Unconventional Oil And Gas Impacts On Downstream Fish Assemblages And Physiological Stress, Joshua Noah Ankeny 2019 West Virginia University

Analysis Of Unconventional Oil And Gas Impacts On Downstream Fish Assemblages And Physiological Stress, Joshua Noah Ankeny

Graduate Theses, Dissertations, and Problem Reports (ETD)

Unconventional Oil and Gas (UOG) production has been steadily expanding throughout the mid-Atlantic since 2008. Increased sedimentation, degraded water chemistry and an overall decrease in habitat quality due to UOG is anticipated to negatively impact aquatic inhabitants, a common observation in other stressed landscapes (i.e., mining, agriculture, development). We assessed stream health through both community analysis and physiological parameters (i.e., growth and hematocrit). A before-after-control-impact study uncovered three fish metrics that were significantly different following UOG disturbances. The invertivore-piscivore metric decreased following UOG disturbance (p = 0.045) whereas two benthic metrics saw a surprising increase (p = 0.003 and p …


Drought And Coyotes Mediate The Relationship Between Mesopredators And Human Disturbance In California, Molly K. Parren 2019 Cal Poly Humboldt

Drought And Coyotes Mediate The Relationship Between Mesopredators And Human Disturbance In California, Molly K. Parren

Cal Poly Humboldt theses and projects

Mesopredators in California are facing two major changes to their ecosystem: drought and the expansion of human disturbance. As a result, mesopredators are likely shifting their habitat use as well as their interspecies interactions to balance resource needs and risk-taking on the landscape. In response to severe drought, the California Department of Fish and Wildlife deployed 585 camera traps throughout the Mojave Desert and Central Valley documenting mammalian mesopredator presence in a drought year (2016) and a post-drought year (2017). The objectives of this study were to examine spatial patterns of mesopredator occurrence and co-occurrence with a dominant predator, the …


Distribution And Habitat Use Of Benthic Fishes In The Lower Kanawha River, West Virginia, Nathaniel V. Owens 2019 West Virginia University

Distribution And Habitat Use Of Benthic Fishes In The Lower Kanawha River, West Virginia, Nathaniel V. Owens

Graduate Theses, Dissertations, and Problem Reports (ETD)

The Kanawha River in West Virginia is a historically diverse system in terms of ichthyofauna although, like many other large rivers, it has been subjected to a suite of anthropomorphic perturbations. These include this system being modified to allow the passage of commercial vessels for industry thus altering flow regimes, increased sedimentation, water depth, and channelization, and decreased interactions with its floodplain. Additionally, the fishes of this system have suffered from deleterious historical water quality issues caused by intensive timber operations, mining, and industrial waste. Post Clean Air Act, Clean Water Act, and Surface Mining Control and Reclamation Act water …


Impact Of Seasonal And Host-Related Factors On The Intestinal Microbiome And Cestode Community Of Sorex Cinereus And Sorex Monticola, Katelyn D. Cranmer 2019 Georgia Southern University

Impact Of Seasonal And Host-Related Factors On The Intestinal Microbiome And Cestode Community Of Sorex Cinereus And Sorex Monticola, Katelyn D. Cranmer

College of Graduate Studies: Theses & Dissertations

The intestinal microbiome of mammals plays a significant role in host health and response to environmental stimuli and can include both beneficial native bacteria as well as parasitic worms. In this study, I examined the intestinal cestode and bacterial communities of two closely related species of shrew, Sorex monticola and Sorex cinereus, over a six month period in 2016. Specimens were collected approximately every three weeks from May to October from the Sangre de Cristo Mountains in Cowles, New Mexico. A total of 79 shrews were prepared with the gastrointestinal tracts removed and flash-frozen in liquid nitrogen. An additional …


A Multi-Scale Analysis Of Jaguar (Panthera Onca) And Puma (Puma Concolor) Habitat Selection And Conservation In The Narrowest Section Of Panama., Kimberly A. Craighead 2019 Antioch University of New England

A Multi-Scale Analysis Of Jaguar (Panthera Onca) And Puma (Puma Concolor) Habitat Selection And Conservation In The Narrowest Section Of Panama., Kimberly A. Craighead

Antioch University Dissertations & Theses

Over the past two centuries, large terrestrial carnivores have suffered extreme population declines and range contractions resulting from the synergistic anthropogenic threats of land-use change and indirect effects of climate change. In Panama, rapid land use conversion coupled with climate change is predicted to negatively impact jaguar (Panthera onca) and puma (Puma concolor). This dissertation examined the environmental variables and scales influencing jaguar and puma habitat selection by season (annual, wet, and dry), using multi-scale optimized habitat suitability models and a machine-learning algorithm (Random Forests), in the narrowest section of Panama. The models derived from the data of an intensive …


Habitat Characteristics And Nesting Ecology Of Golden Eagles In Arizona, Michele J. Losee 2019 Antioch University of New England

Habitat Characteristics And Nesting Ecology Of Golden Eagles In Arizona, Michele J. Losee

Antioch University Dissertations & Theses

Golden Eagles (Aquila chrysaetos) have a broad range globally and in general are well-studied. However, Arizona’s Golden Eagle population remained essentially unstudied until 2011, when Arizona Game and Fish Department (AZGFD) began nest surveys for cliff nesting Golden Eagles throughout the state. As a result of this data collection, the natural history of Arizona’s Golden Eagles is finally revealing itself. This dissertation outlined a reliable description of their nesting phenology that provides a framework for timing surveys and a baseline to monitor the effects of climate change on Golden Eagles. The mean date for egg-laying was February 14 and pairs …


Just Preservation, Adrian Treves, Francisco J. Santiago-Ávila, William S. Lynn 2019 University of Wisconsin-Madison

Just Preservation, Adrian Treves, Francisco J. Santiago-Ávila, William S. Lynn

Animal Sentience

We are failing to protect the biosphere. Novel views of conservation, preservation, and sustainability are surfacing in the wake of consensus about our failures to prevent extinction or slow climate change. We argue that the interests and well-being of non-humans, youth, and future generations of both human and non-human beings (futurity) have too long been ignored in consensus-based, anthropocentric conservation. Consensus-based stakeholder-driven processes disadvantage those absent or without a voice and allow current adult humans and narrow, exploitative interests to dominate decisions about the use of nature over its preservation for futurity of all life. We propose that authentically non-anthropocentric …


Using Lidar To Approximate Keystone Structure And Evaluate Management Practices In Potential Habitats Of The Endangered Karner Blue Butterfly (Lycaeides Melissa Samuelis), Brandyn Balch 2019 West Virginia University

Using Lidar To Approximate Keystone Structure And Evaluate Management Practices In Potential Habitats Of The Endangered Karner Blue Butterfly (Lycaeides Melissa Samuelis), Brandyn Balch

Graduate Theses, Dissertations, and Problem Reports (ETD)

Keystone structure is the spatial structure required by a given species, at a scale that is determined by that species’ needs and mobility. The endangered Karner blue butterfly (Lycaeides melissa samuelis, hereafter KBB) has a keystone structure that incorporates trees and bushes to provide the mixture of sun and shade required to fulfil its life functions. Airborne light detection and ranging (lidar) is a potentially invaluable tool for characterizing keystone structures. However, lidar has yet to be utilized to evaluate structural suitability of KBB habitats. Therefore, I investigated the use of lidar for characterizing critical attributes of KBB habitat structure, …


Cerulean Warbler (Setophaga Cerulea) And Associated Species Response To Operational Silviculture In The Central Appalachian Region, Gretchen Emily Nareff 2019 West Virginia University

Cerulean Warbler (Setophaga Cerulea) And Associated Species Response To Operational Silviculture In The Central Appalachian Region, Gretchen Emily Nareff

Graduate Theses, Dissertations, and Problem Reports (ETD)

In this study, I assessed the response of Cerulean Warblers (Setophaga cerulea) and 5 additional songbird species to timber harvests prescribed through operational silviculture. The research took place in relatively contiguous mature deciduous forests in 4 states in the central Appalachian region—Kentucky, Pennsylvania, Virginia, and West Virginia, USA.

For the first part of the study, I collected Cerulean Warbler abundance and territory data through point counts and territory mapping, respectively. I used the point count data to model Cerulean Warbler abundance pre- and post-harvest at 5 study areas (Kentucky [n=1], Virginia [n=2], West Virginia [n=2]) and post-harvest at …


Human-Wildlife Conflict In And Around Borena Sayint National Park, Northern Ethiopia, Ayenew Biset, Girma Mengesha, Zerihun Girma 2019 Hawassa University

Human-Wildlife Conflict In And Around Borena Sayint National Park, Northern Ethiopia, Ayenew Biset, Girma Mengesha, Zerihun Girma

Human–Wildlife Interactions

We identified causes, impact, and traditional management measures of human– wildlife conflict (HWC) in and around Borena Sayint National Park, Ethiopia. We employed questionnaires, focus group discussions, direct observations, and key informant interviews to collect data. The respondents perceived an increase in the number of wildlife population (56 respondents; 42.4%) followed by human proximity to park areas (44 respondents; 33.3%) as the main causes of HWC. The respondents perceived leopards (Panthera pardus) and spotted hyenas (Crocuta crocuta) among the top livestock depredators while grivet monkeys (Cercopethicus aethiops) and porcupines (Hystrix cristata) were perceived …


Train–Elephant Collisions In A Biodiversity-Rich Landscape: A Case Study From Rajaji National Park, North India, Ritesh Joshi, Kanchan Puri 2019 Ministry of Environment Forest and Climate Change Indira Paryavaran Bhawan Jor Bagh Road New Delhi

Train–Elephant Collisions In A Biodiversity-Rich Landscape: A Case Study From Rajaji National Park, North India, Ritesh Joshi, Kanchan Puri

Human–Wildlife Interactions

Linear developments like railways and highways have a negative impact on ecological processes of wildlife species at a landscape level. The impacts in terms of wildlife mortality and threat to surviving populations of species have been well-studied; however, less work has been done to understand the potential causes of train–wildlife collisions, particularly large mega-fauna such as Asian elephants (Elephas maximus; elephant). In this case study, we review train–elephant collisions (TECs) that occurred in Rajaji National Park (RNP) and discuss some potential causes of TECs along with mitigation measures. The RNP, located in the upper Gangetic plains of northern …


Three Sister Crops: Understanding American Indian Agricultural Practices Of Corn, Beans And Squash, Sara Colombe, Madhav P. Nepal, Larry B. Browning, Matthew L. Miller, P. Troy White 2018 Hoven High School, Hoven, South Dakota

Three Sister Crops: Understanding American Indian Agricultural Practices Of Corn, Beans And Squash, Sara Colombe, Madhav P. Nepal, Larry B. Browning, Matthew L. Miller, P. Troy White

iLEARN Teaching Resources

American Indians have practiced an inter-planting system to produce corn, beans, and squash, for generations. These crops are known as the “Three Sisters”. In this lesson developed for secondary agriscience curriculum, students will understand the past, current and future production practices of the three important crops. Students will also apply their knowledge to understand the crop selection process and relate to the changing environment.


Understanding The Impacts Of Current And Future Environmental Variation On Central African Amphibian Biodiversity, Courtney A. Miller 2018 LSU New Orleans

Understanding The Impacts Of Current And Future Environmental Variation On Central African Amphibian Biodiversity, Courtney A. Miller

LSU New Orleans Theses and Dissertations

Global climate change is projected to impact multiple levels of biodiversity by imposing strong selection pressures on existing populations, triggering shifts in species distributions, and reorganizing entire communities. The Lower Guineo-Congolian region in central Africa, a reservoir for amphibian diversity, is predicted to be severely affected by future climate change through rising temperatures and greater variability in rainfall. Geospatial modelling can be used to assess how environmental variation shapes patterns of biological variation – from the genomic to the community level – and use these associations to predict patterns of biological change across space and time. The overall goal of …


Scorpions Of The Horn Of Africa (Arachnida, Scorpiones). Part Xvii.Revision Of Neobuthus, With Description Of Seven New Species From Ethiopia, Kenya And Somaliland (Buthidae), František Kovařík, Graeme Lowe, Ahmed Ibrahim Awale, Hassan Sh Abdirahman Elmi, Ali Abdi Hurre 2018 Marshall University

Scorpions Of The Horn Of Africa (Arachnida, Scorpiones). Part Xvii.Revision Of Neobuthus, With Description Of Seven New Species From Ethiopia, Kenya And Somaliland (Buthidae), František Kovařík, Graeme Lowe, Ahmed Ibrahim Awale, Hassan Sh Abdirahman Elmi, Ali Abdi Hurre

Euscorpius

New information about the taxonomy and distribution of the genus Neobuthus Hirst, 1911 is presented, based on material recently collected mainly from Somaliland, but also Djibouti and Kenya. Emended diagnoses are proposed for N. berberensis Hirst, 1911 and N. ferrugineus (Kraepelin, 1898) sensu stricto. New species described are: N. amoudensis sp. n. from Ethiopia and Somaliland; N. erigavoensis sp. n., N. factorio sp. n., N. gubanensis sp. n., N. maidensis sp. n., and N. montanus sp. n. from Somaliland; and N. kloppersi sp. n. from Kenya. This doubles the number of species in Neobuthus, unearthing a rich diversification …


Revision Of The Central Asian Scorpion Genus Anomalobuthus Kraepelin, 1900, With Descriptions Of Three New Species And A Generic Synonymy (Scorpiones: Buthidae), Rolando Teruel, František Kovařík, Victor Fet 2018 Marshall University

Revision Of The Central Asian Scorpion Genus Anomalobuthus Kraepelin, 1900, With Descriptions Of Three New Species And A Generic Synonymy (Scorpiones: Buthidae), Rolando Teruel, František Kovařík, Victor Fet

Euscorpius

We revise the Central Asian endemic genus Anomalobuthus Kraepelin, 1900, which was considered monotypic for more than 100 years until the recent addition of a second species from Iran (Teruel et al., 2014). We redefine the generic diagnosis of Anomalobuthus and reveal that it is composed of no less than six species, three of which are described as new: A. krivochatskyi, sp. n. (central Uzbekistan and extreme southern Kazakhstan), A. lowei, sp. n. (southeastern Kazakhstan), and A. pavlovskyi, sp. n. (south-central Kazakhstan and extreme northern Turkmenistan). The monotypic genus Psammobuthus Birula, 1911 (described from the Ferghana Valley …


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