Life Sciences Commons^™

Extinct Or Extant? A New Species Of Termitodius Wasmann, 1894, (Coleoptera: Scarabaeidae: Aphodiinae: Rhyparini) With A Short Review Of The Genus, Paul E. Skelley, Julian Clavijo-Bustos, Oliver Keller

Insecta Mundi

A new species of Termitodius Wasmann (Coleoptera: Scarabaeidae: Aphodiinae: Rhyparini) is de­scribed from Colombia, Termitodius woodruffi Skelley, Clavijo-Bustos, and Keller, new species. This species is both extant and abundantly preserved in copal. The genus Termitodius is reviewed with a key and brief accounts to all species.

Una nueva especie de Termitodius Wasmann (Coleoptera: Scarabaeidae: Aphodiinae: Rhyparini) es descrita de Colombia, Termitodius woodruffi Skelley, Clavijo-Bustos, y Keller, nueva especie. Esta especie es existente y abundantemente preservada en copal. El género Termitodius es revisado con una clave y reseñas breves para todas las especies.

The pantropical tribe Rhyparini is represented …

Phenotypical Characters Associated With The Loss Of The Seed And The Production Of Biomass In Panicum Coloratum Var. Makarikariense, Maria F. Moresco Lirusso, Mónica B. Sacido, Guillermo R. Pratta, Silvina A. Felitti

IGC Proceedings (1977-2023)

Since the beginning of humanity, plants have been manipulated by humans by artificial selection to obtain changes in their characteristics. This progress has been achieved through the domestication of the species. Panicum coloratum is a species of summer perennial grass, megathermic, tolerates waterlogging, cold and slightly saline soils. It is of African origin and used as fodder in various parts of the world. In Argentina around 130.500 hectares are sown, the most widespread varieties are: var. coloratum, and var. makarikariense Goossens. Its characteristics of determined flowering and ununiform ripening within the panicle, added to a very low retention of …

The Role Of Bacteria And Fungi On Forage Degradation In Vitro, Gilberto V. Kozloski, D. C. Rösler, C. A. Pozo, L. E. Leonardi, Mariana P. Mezzomo

IGC Proceedings (1977-2023)

The study was conducted to evaluate the interactive role of bacteria and fungi on forage degradation in vitro. Samples of Cynodon spp. were incubated in a 48-h in vitro gas assay with incubation medium containing or not antimicrobial substances. Treatments were: antibiotic (Ab), antifungal (Af), negative control (i.e. without antimicrobials) or positive control (i.e. with both Ab and Af). Three replicate assays were conducted and, in each assay the gas volume was measured at 3, 6, 9, 12, 24, 36 and 48 h of incubation. Data of cumulative gas production in each flask in each assay was fitted to …

Performance Of Rotational Grazing Of Urochloa Hybrid Cv. Cayman In The Caribbean Region Of Costa Rica, M. Hernández-Chaves, R. Van Der Hoek, S. Abarca-Monge, R. Soto-Blanco, J. Arango

IGC Proceedings (1977-2023)

In Costa Rica pasture-based grass is the basis for both dairy and beef production. The Caribbean region with a rainfall exceeding 3000 mm has four seasons: dry (Feb-May), rainy (May-Aug), less rainy (Sep-Oct) and intermediate (Oct-Feb). Many pastures are dominated by Ischaemum ciliare (“Retana”), of poor nutritional quality and permanently grazed with low stocking rates (one animal per ha). To assess the potential of combining improved grasses with rotational grazing, a trial with Urochloa hybrid cv. Cayman was implemented at the INTA research station in Guápiles, Limón province, during four years (2014 to 2018). An intensive pasture management system based …

Comparative Analysis Of The Nutritional, Biochemical And Hematological Parameters Of Pregnant Women Attending The University Of Abuja Teaching Hospital, Nigeria, Anthony Uchenna Emeribe, Amos Dangana, Hezekiah Alkali Isa, Solomon Elochi Onoja, Theresa Ohunene Otu, Yakubu Ibrahim, Amilia Afzan Mohammad Jamil, Moses D. Lugos, Dorcas Aliyu, Shamsudeen Haruna, Mala Alhaji Baba Mallam, Hadiza Yahaya, Maijidda Aminu Saidu, Silifat Oyewusi, Mustapha Bakare, Idris Nasir Abdullahi, Justin Onyebuchi Nwofe

BioMedicine

Background: Despite the efforts to encourage the intake of nutritional supplements during antenatal periods, there are still many cases of anemia and protein-energy malnutrition during pregnancy. Hence, this study determined the incidence of anemia, protein-energy malnutrition, and associated risk factors among pregnant women in Abuja, Nigeria.

Materials and Methods: This hospital-based, case-control study involved randomly selected 176 pregnant and non-pregnant women attending the University of Abuja Teaching Hospital (UATH), Gwagwalada, Nigeria. Hemoglobin and hematocrit measurements were used to determine anemia incidence, while plasma protein, zinc levels and body mass index (BMI) were used to determine energy index status. …

Effect Of Rehmannia Glutinosa Libosch Extract On Proliferation And Cardiogenic Pre-Differentiation Of Human Mesenchymal Stem Cells, Huu Dat Nguyen, Len Ho Thi, Xuan Bach Ho, Van Anh Cao, Duy Minh Le Hoang

BioMedicine

Background: Vietnamese medicine tried and tested certain bioactive compounds from plants to increase the rate of tissue immunomodulation, regeneration, and differentiation. Although there are many research papers discovered about phytochemicals of Rehmannia glutinosa Libosch and differentiation induction potential of some substances purified from this herbal, it finds difficult to seek research that investigated the effect of Rehmannia glutinosa Libosch extract on proliferation and cardiogenic differentiation of mesenchymal stem cells, even though it has commonly been used for a long time because of its function as a restorative and as a critical role in cardiovascular treatment in traditional.

Results: Our …

Anti-Herpes Virus Activity Of Lactobacillus’ Postbiotics, Neli Vilhelmova_Ilieva, Georgi Atanasov, Lora Simeonova, Lili Dobreva, Kapka Mancheva, Madlen Trepechova, Svetla Danova

BioMedicine

Background: Recently, various lactic acid bacteria (LAB) and their post-metabolites, have shown many positive effects on human and animal welfare. They appear to be beneficial in different disorders and pathological conditions, including in a broad-spectrum of infectious diseases.

Aim: To estimate in vitro the anti-herpes simplex activity of 11 postbiotic samples (lysates or cell-free supernatants - CFS), produced during the fermentation of six candidate-probiotic Lactobacillus strains, isolated from Bulgarian fermented milk products.

Materials and methods: In vitro protocols for assessment of different LAB samples on the Herpes simplex virus type 1 (HSV-1) replication, adsorption and virucidal effects were applied using …

The Effect Of Heparin Concentration On Results Of Venous Blood Gas Of Patients Admitted To Cardiac Intensive Care Unit: A Double-Blind Clinical Trial, Fatemeh Shima Hadipourzadeh, Rasool Lakziyan, Fidan Shabani, Zohreh Sarchahi, Saeideh Mazloomzadeh

BioMedicine

Background: the aim of the present study was to investigate the effect of heparin (1000 IU/mL) in the blood sample on the results of venous blood gases of patients admitted to the cardiac intensive care unit.

Materials and Methods: The present double-blind randomized clinical trial study was performed on 282 samples from 141 patients admitted to the cardiac intensive care unit. Insulin syringes with heparin (1000 IU/mL) and heparin (5000 IU/mL) and 1 cc of blood sample were taken from the peripheral vein, then distributed in two syringes and given to the analyzer.

Results: In the present …

Applying Artificial Neural-Network Model To Predict Psychiatric Symptoms, Elaheh Allahyari, Narges Roustaei

BioMedicine

Introduction: Mental disorders result in mental disabilities and discomfort in the affected person as they affect both thinking and behavior. Therefore, more vulnerable people must first be identified to improve the psychological level of society.

Aim: This study aims to determine the importance of gender, employment, education, place of residence, and age in predicting mental disorders using artificial neural network modeling.

Methods: The pattern held between variables in this study will be identified using multilayer feed-forward back-propagation neural networks with five inputs and 10 outputs. To determine the neural network with the least sum of square errors, we evaluated the …

First Record Of The Genus Compsobuthus (Scorpiones: Buthidae) From Maharashtra, India, With Description Of A New Species, Vivek Waghe, Satpal Gangalmale, Akshay Khandekar

Euscorpius

A new species of the genus Compsobuthus Vachon, 1949 (Buthidae) belonging to the werneri group is described from two localities in Jalgaon District, Maharashtra, India. The new species can be easily distinguished from all four members of the werneri group known from India and Pakistan by combination of nonoverlapping morphological characters. Compsobuthus satpuraensis sp. n. is the first species of this genus to be reported from the state of Maharashtra and fourth to be reported from India.

Addition Of Inexpensive Lights To Aquatic Turtle Traps Improves Trapping Efficiency In Early Spring, Jenna B. Myers, Michael J. Bender, Maggie M. Woodall*, Jennifer L. Mook

Georgia Journal of Science

Aquatic turtles are essential contributors to many freshwater ecosystems, but they face a myriad of threats, necessitating periodic monitoring of population status. Increasing turtle trapping efficiency has the potential to improve conservation efforts, particularly when population sizes are low or sampling conditions are suboptimal. In an effort to improve trapping efficiency, we added LED lights to turtle traps in an attempt to attract kinosternid turtles. Our investigation into the effectiveness of LED lights as an attractant was based on evidence suggesting that these turtles may forage using both visual and olfactory cues. Lights significantly increased captures of kinosternid turtles during …

Role Of Bpifb4 In Immune System And Cardiovascular Disease: The Lesson From Centenarians, Francesco Montella, Valentina Lopardo, Monica Cattaneo, Albino Carrizzo, Carmine Vecchione, Elena Ciaglia, Annibale A. Puca

Translational Medicine @ UniSa

Recent discoveries have shed light on the participation of the immune system in the physio pathology of the cardiovascular system underpinning the importance of keeping the balance of the first to preserve the latter. Aging, along with other risk factors, can challenge such balance triggering the onset of cardiovascular diseases.

Among several mediators ensuring the proper cross-talk between the two systems, bactericidal/permeability-increasing fold-containing family B member 4 (BPIFB4) has been shown to have a pivotal role, also by sustaining important signals such as eNOS and PKC-alpha.

In addition, the Longevity-associated variant (LAV), which is an haplotype allele in BPIFB4characterized by …

Making The Case For Rangelands To Become The Poster Child Of The Sdgs: How Rangelands Provide Fertile Ground For An Interconnected Approach To The Three Pillars Of Sustainable Development, B. Jones

IGC Proceedings (1977-2023)

The Sustainable Development Goals (SDGs) are a collection of 17 ambitious goals set by global leaders to transform the world by 2030 and create a sustainable future for all. They are founded on the three pillars of sustainable development – environmental, economic and social sustainability, or as they are more colloquially known, “people, planet and profit”. Creating a better world requires an integrated approach that simultaneously improves livelihoods and reduces inequalities whilst dramatically reducing environmental damage.

This paper argues that rangelands are an ideal arena for showcasing the SDGs because they are, by their very nature, interconnected landscapes in which …

Intellectual Property Protection – Stimulating Or Constraining Innovation And Technology Transfer?, John R. Caradus, C. A. Tumilson, J. Y. Lin

IGC Proceedings (1977-2023)

Grassland farming is becoming more technically advanced leading to improvements in productivity, environmental outcomes and animal welfare. As a result, farmers have an increasing range of new innovations being made available to them. The question examined here is whether widespread uptake of new innovations by farmers is more effective when driven by marketing through dedicated paths to market where the intellectual property (IP) is controlled or through multiple paths to market where there is no IP control?

The role of IP in stimulating the development, manufacture and sale of new technologies is hotly debated. For plant breeders and patent owners, …

High Altitude Rangelands And Pastoralism In Bhutan: Using Sustainable Development Goals To Address Land Degradation And Poverty, J. E. Millar, K. Tenzing

IGC Proceedings (1977-2023)

Semi-nomadic pastoralists in Bhutan live in high altitude rangelands where they seasonally migrate with yak and cattle herds from 3,000m to 5,000m. Population increase, overgrazing and climate change in eastern Bhutan have led to severe land degradation, winter fodder shortage and reduced milk production per head for herding families. This paper describes how Sustainable Development Goals (SDGs) were used to design a rangeland rehabilitation and livelihoods improvement program with herders in Merak district from 2016 to 2019. The aim was to restore degraded rangelands, improve pastures, develop savings schemes, resolve conflicts and increase conservation knowledge. The approach involved gender sensitive …

Conversion Of Forest To Agro-Silvo-Pastoral System – Montado – In Mediterranean Environments, J. Potes, F. Jorge, T. Montes, D. Palha

IGC Proceedings (1977-2023)

The Mediterranean environment regions are characterized by climate and soil specificities that justify low productive capacity for primary production when compared to other environments on earth. The Mediterranean forests are essentially formed by trees and shrubs. The shrubs are the perfect fuel to feed the forest fires that occur in a natural and cyclical manner in these environmental conditions. Therefore, the Mediterranean Systems of Agriculture have evolved to control the shrubs and the extensification of the systems because of low productivity. Sustainability is achieved by increase of productivity through improvement of the soil and irrigation. The application of these principles …

Fundulus Parasite Database, Derek Garvey, Christopher Blanar, David Kerstetter

Biology Faculty Datasets

Data gathered from field sampling and literature review of presence/absence and abundance data for all metazoan parasite taxa described in Fundulus spp. populations from sample locations along the Atlantic coasts of the USA and Canada.

REVISION 03-13-2023: This revision added two tabs to the database. The "Taxonomy Key" tab was added to show the classification scheme used to group the observed parasite taxa. The "EndoEcto Key" tab was added to show the classification of each parasite taxon as either endoparasitic or ectoparasitic. The original database file can be found in supplemental content below.

Carbon-Sensitive Pedotransfer Functions For Plant Available Water, Dianna K. Bagnall, Cristine L. S. Morgan, Michael Cope, Gregory M. Bean, Shannon Cappellazzi, Kelsey Greub, Daniel Liptzin, Charlotte L. Norris, Elizabeth Rieke, Paul Tracy, Ezra Aberle, Amanda Ashworth, Oscar Bañuelos Tavarez, Andy Bary, Roland Louis Baumhardt, Alberto Borbón Gracia, Daniel Brainard, Jameson Brennan, Dolores Briones Reyes, Darren Bruhjell, Cameron Carlyle, James Crawford, Cody Creech, Steven Culman, William Deen, Curtis Dell, Justin Derner, Thomas Ducey, Sjoerd Willem Duiker, Miles Dyck, Benjamin Ellert, Martin Entz, Avelino Espinosa Solorio, Steven J. Fonte, Simon Fonteyne, Ann-Marie Fortuna, Jamie Foster, Lisa Fultz, Audrey V. Gamble, Charles Geddes, Deirdre Griffin-Lahue, John Grove, Stephen K. Hamilton, Xiying Hao, ‪Zachary D. Hayden, Julie Howe, James Ippolito, Gregg Johnson, Mark Kautz, Newell Kitchen, Sandeep Kumar, Kirsten Kurtz, Francis Larney, Katie Lewis, Matt Liebman, Antonio Lopez Ramirez, Stephen Machado, Bijesh Maharjan, Miguel Angel Martinez Gamiño, William May, Mitchel Mcclaran, Marshall Mcdaniel, Neville Millar, Jeffrey P. Mitchell, Philip A. Moore, Amber Moore, Manuel Mora Gutiérrez, Kelly A. Nelson, Emmanuel Omondi, Shannon Osborne, Leodegario Osorio Alcalá, Philip Owens, Eugenia M. Pena-Yewtukhiw, Hanna Poffenbarger, Brenda Ponce Lira, Jennifer Reeve, Timothy Reinbott, Mark Reiter, Edwin Ritchey, Kraig L. Roozeboom, Ichao Rui, Amir Sadeghpour, Upendra M. Sainju, Gregg Sanford, William Schillinger, Robert R. Schindelbeck, Meagan Schipanski, Alan Schlegel, Kate Scow, Lucretia Sherrod, Sudeep Sidhu, Ernesto Solís Moya, Mervin St. Luce, Jeffrey Strock, Andrew Suyker, Virginia Sykes, Haiying Tao, Alberto Trujillo, Laura L. Van Eerd, Nele Verhulst, Tony John Vyn, Yutao Wang, Dexter Watts, David Wright, Tiequan Zhang, Charles Wayne Honeycutt

Agricultural and Environmental Sciences Faculty Research

Currently accepted pedotransfer functions show negligible effect of management-induced changes to soil organic carbon (SOC) on plant available water holding capacity (θAWHC), while some studies show the ability to substantially increase θAWHC through management. The Soil Health Institute's North America Project to Evaluate Soil Health Measurements measured water content at field capacity using intact soil cores across 124 long-term research sites that contained increases in SOC as a result of management treatments such as reduced tillage and cover cropping. Pedotransfer functions were created for volumetric water content at field capacity (θFC) and permanent wilting point (θPWP). New pedotransfer functions had …

A New Species Of Brachycerocoris Costa (Hemiptera: Heteroptera: Pentatomidae: Podopinae) From The Philippines, Joseph E. Eger Jr.

Insecta Mundi

A new species, Brachycerocoris woodruffi Eger, is described from Mindanao Island, Philippines. The new species is described, illustrated and compared to the two other species of Brachycerocoris Costa occurring in the Philippines, B. dromedarius (Vollenhoven) and B. davidii Roca-Cusachs and Salini.

Brachycerocoris Costa belongs to the Brachycerocoris group of the Podopinae (Rider et al. 2018) and is represented in Africa by three species, B. afer Stål 1876, B. congoanus Schouteden 1905, and B. patrizii Mancini 1939. The genus has historically contained just two species in the Oriental region, B. camelus Costa 1863, described from China, and B. dromedarius (Vollenhoven 1863), …

Long-Term Sustainability Implications Of Diverse Commercial Pollinator Mixtures For The Conservation Reserve Program, Amanda J. Ashworth, Taylor Adams, Alayna Jacobs

Plant and Soil Sciences Graduate Research

The persistence of Conservation Reserve Program (CRP) seed mixtures following planting is crucial for the long-term support of pollinator habitat; however, the role of disturbance regimes and their interactions in supporting this ecosystem service are largely unknown. This study set out to evaluate how ecological disturbances (prescribed burn, light disking, or fallow control) and commercially available diverse species mixtures (‘Hamilton’, ‘Bamert’, and ‘Holland’) affect pollinator habitat and the flowering period of commonly used CRP species mixtures. Specifically, three pollinator species mixtures were assessed for plant stand dynamics (plant density, yield, and dual-use pollinator-lignocellulosic feedstock potential); the resulting soil properties; and …

Evaluating Split Timing Fertilizer Applications For Improving Bread Baking Quality Of Soft Red Winter Wheat In Kentucky, Ammar Al Zubade, Krista Jacobsen, Tim Phillips, Mark Williams, David Van Sanford

Plant and Soil Sciences Graduate Research

There is growing interest among farmers to locally produce high protein and strong gluten wheat that is suitable for bread making and meet the demand of local artisanal bakers in Kentucky. The warm and humid weather in southeast region is ideal for soft red winter wheat (SRW) production which characterized by low protein content. The technique of splitting nitrogen (N) fertilization according to the growth stages has been suggested to improve protein content and its composition. This study evaluated the effect of split N application on yield and baking quality traits of two SRW wheat cultivars grown in the eastern …

Identifying Molecular Markers For Early Detection Of Toxic Cyanobacteria And Dinoflagellate, Shafqat F. Ehsan

Honors Program Theses and Research Projects

Harmful algal Blooms (HABs) develop when algal colonies grow out of control, causing toxicity or injury to humans, fish, shellfish, marine mammals, and birds. Most HABs of public health concern in saltwater generally are caused by eukaryotic dinoflagellates and diatoms. Prokaryotic cyanobacteria are usually responsible for freshwater blooms although they can contribute to saltwater and brackish blooms too. A common monitoring target of both groups is the saxitoxin-encoding genes. Saxitoxin(STX) is responsible for Paralytic shellfish poisoning, a foodborne illness developed from consumption of STX contaminated shellfish. Each cyanobacterial SXT gene cluster contains a set of core genes, common to all …

Not Only Severe Events: Moderate Dry Periods Impact The Hydraulic Functioning And Survival Of Planted Ponderosa Pine Seedlings, Carolyn R. Koehn, Matthew D. Petrie, Robert M. Hubbard

Life Sciences Faculty Research

Juvenile tree survival will increasingly shape the persistence of ponderosa pine forests in the western United States. In contrast to severe pulse disturbances that induce widespread adult and juvenile tree mortality, moderate periods of low rainfall and warm temperatures may reduce forest persistence by killing juvenile trees at the seedling stage. Intensification of these periods in a changing climate could therefore increasingly restrict both natural regeneration and artificial regeneration of planted seedlings. We conducted a controlled field experiment at a single site in the Front Range of Colorado, USA, to determine the responses and survival of 3 Colorado subpopulations of < 1 year old potted ponderosa pines to moderately dry conditions, variation in small rainfall events based on observed patterns, and shaded and unshaded microsite environments. Near surface soil moisture increased slightly following small rainfall events, but declined over the 45-day experimental period. Seedling transpiration and associated canopy cooling declined after ∼13 days, and further declines in transpiration and canopy cooling suggest that the majority of trees in lower rainfall treatments experienced hydraulic dysfunction between days ∼20–30. After 45 days, mortality across all subpopulations and treatments, inferred by relative water loss, exceeded 90–95%. Despite some uncertainty pertaining to the stress tolerance of nursery grown versus naturally germinated conifers, our results show that planted ponderosa pine seedlings < 1 year old are unlikely to survive moderate dry periods of 20+ days relying on small rainfall events. Although microsite conditions and soil moisture availability shaped tree hydraulic functioning early in the experiment (days 1–13), later functioning was shaped predominately by the legacy of rainfall treatments. Our results illustrate the importance of moderate dry events that occur consistently as part of seasonal variation in climate, and show how their intensification may constitute a sustained press that limits opportunities for natural and artificial regeneration.

Lost Grounds In Us Trade Integration, John C. Beghin

Cornhusker Economics

Growing World Trade Organization (WTO) membership and plurilateral trade agreements have increasingly occurred in the last 20 years leading to unprecedented market integration. However, in the last 5 years, the United States (US) has been diverging from its traditional role as a main driver of this integration. In contrast, Europe has been integrated progressively within itself and beyond, outpacing the US. The article documents these trends and changes. This article is based on a longer working paper and an article in EuroChoices coauthored with Jill O’Donnell.

Taxonomic And Phylogenetic Diversity Of Pitcher Plant Bogs In Georgia's Coastal Plain, Melanie Flood

Theses and Dissertations

Diversity studies via floristic survey and taxonomic metrics have proven essential for discerning community composition and biodiversity, however, phylogenetic analyses are necessary to reveal underlying evolutionary change and potential community assembly patterns. Pitcher plant bogs are highly diverse, highly understudied habitats that contain numerous listed and at-risk plant and animal species of Georgia.

In this study I investigated the taxonomic and phylogenetic diversity of pitcher plant bogs by asking the following questions:

1) What is the taxonomic diversity of pitcher plant bogs,

2) What is the dispersion of phylogenetic diversity within bog habitats, and

3) Does taxonomic and phylogenetic diversity …

New Fossil False Click-Beetles From The Americas (Coleoptera: Eucnemidae), Jyrki Muona

Insecta Mundi

One new genus and three new false click-beetle species (Coleoptera: Eucnemidae) are described from American fossil resins: Neusiokia new genus, type species Neusiokia appalachiensis new species (North Carolina resin), Thambus woodruffi new species (Dominican resin) and Asiocnemis colombicus new species (Colombian resin).

Seventy-two fossil species are included in the family Eucnemidae presently. Half of them, 36, are based on Baltic amber materials (Muona 1993a; Muona 2021), 22 are from Myanmar amber deposits (Li et al. 2020; Muona 2020; Otto 2019) and 10 are mineral fossils, nine of these from China (Chang et al. 2016; Muona et al. 2020; Li et …

Bacterial Indole As A Multifunctional Regulator Of Klebsiella Oxytoca Complex Enterotoxicity, Nagender Ledala, Mishika Malik, Karim Rezaul, Sara Paveglio, Anthony Provatas, Aaron Kiel, Melissa Caimano, Yanjiao Zhou, Jonathan Lindgren, Kristyna Krasulova, Peter Illes, Zdenek Dvorak, Sandhya Kortagere, Sabine Kienesberger, Amar Cosic, Lisa Pöltl, Ellen L. Zechner, Subho Ghosh, Sridhar Mani, Justin D. Radolf, Adam P. Matson

Faculty Research 2022

Gastrointestinal microbes respond to biochemical metabolites that coordinate their behaviors. Here, we demonstrate that bacterial indole functions as a multifactorial mitigator of Klebsiella grimontii and Klebsiella oxytoca pathogenicity. These closely related microbes produce the enterotoxins tilimycin and tilivalline; cytotoxin-producing strains are the causative agent of antibiotic-associated hemorrhagic colitis and have been associated with necrotizing enterocolitis of premature infants. We demonstrate that carbohydrates induce cytotoxin synthesis while concurrently repressing indole biosynthesis. Conversely, indole represses cytotoxin production. In both cases, the alterations stemmed from differential transcription of npsA and npsB, key genes involved in tilimycin biosynthesis. Indole also enhances conversion of tilimycin …

A Tale Of Two-Hybrids: Investigating The Interactomes Of Cullin-Associated Proteins, Elyse Reitter

Dissertations and Theses

Cul3 is the major component of an E3 ligase in human cells. Cul3 was initially identified in the Singer lab as a protein that binds and degrades cyclin E, and subsequent studies have shown it to be part of a complex that is involved in a multitude of biological functions. The nature of this complex, its constituents, its regulation, and its dynamics is just beginning to be understood. The research presented here utilizes a series of two hybrid screens to identify families of interactomes with Cul3 at the center. This information will complement other work in the lab in which …

Development Of A Multivariate Model With Desirability-Based Optimization For Determination Of Atenolol And Hydrochlorothiazide By Eco-Friendly Hplc Method With Fluorescence Detection, Ekram Hany Mohamed, Ahmed Hemdan,, Naser F. Al-Tannak,

Pharmacy

Determination of a widely used antihypertensive combination of atenolol and hydrochlorothiazide was achieved by rapid and eco-friendly high-performance liquid chromatography method combined with fluorescence detection. The response surface methodology is conducive to the complete separation of the two drugs in a shorter analysis time. The separation of the mixture was achieved using an Inertsil C18 analytical column (150 × 4.6 mm, 5 µ). The mobile phase used was ethanol: potassium dihydrogen phosphate at pH 3 (65:35 v/v) and the flow rate was 0.7 mL/min. The fluorescence detector operated at excitation and emission wavelengths of 230 and 310 nm (atenolol) and …

The Planted Catalog: The Role Of The Botanical Garden In Botany, Douglas Tuers

Journal of the South Carolina Academy of Science

This article will investigate the role of the botanical garden in botany. What its role is and how it fills it. This article will take the Missouri Botanical Garden as a case study, drawing heavily from archival material compromised mostly of letters between Henry Shaw and the prominent botanists of his day. This study also draws on the prominent histories of the Missouri Botanical Garden. The analysis use the philosophical tradition around reference in logic and language. This study argues that the botanical garden is a bridge between locality and herbaria. The order of the flora travels to the locality …