Life Sciences Commons^™

The Shoreline Protection Potential Of Oyster Reefs: A Systematic Review And Meta-Analysis, Jessica R. Fergel, Robert E. Isdell, Gabriella Dipetto, Karinna Nunnez, Sean T. Gregory, Evan Hill, Donna Marie Bilkovic

Biological Sciences Faculty Publications

Nature-based solutions for erosion control that incorporate oyster reefs, alone or in combination with other habitats, are an increasingly popular approach due to their potential to protect shorelines and enhance oyster production. However, the extent to which natural or constructed oyster reefs provide shoreline protection remains unclear. We conducted a global systematic literature review and meta-analysis to summarize and evaluate the potential of oyster reefs in attenuating waves, promoting sediment accretion, and/or reducing shoreline erosion. Factors extracted from studies included shoreline protective measures examined, oyster reef structure type, and oyster reef tidal location. The results of the meta-analysis showed generally …

Ovarian Cancer Immunology And Immunotherapy: Editorial Note, Chris D. Platsoucas

Biological Sciences Faculty Publications

[Introduction] As the guest editor of the Special Issue on Ovarian Cancer Immunology Immunotherapy, I would like to include certain brief comments on the current state of the field and to introduce the Special Issue. Ovarian carcinoma and malignant melanoma are the first human tumors where immune responses, primarily restricted to autologous tumor cells, were first demonstrated. T-cell lines and clones developed in recombinant interleukin-2 (rIL-2) from tumor-infiltrating lymphocytes (TIL) from these tumors exhibited cytotoxicity and/or cytokine production, primarily restricted to autologous tumor cells. The T-cell receptor (TCR) and the CD3 differentiation antigen on effector T cells and HLA on …

Bayesian Multivariate Modelling Of Lone Star (Amblyomma Americanum) Tick Life Stage Abundance And Temporal Trends To Inform Public Health Risk In Virginia, Thabo Lephoto, Henry Mwambi, Oliver Bodhlyera, Holly Gaff

Biological Sciences Faculty Publications

The increasing abundance of ticks poses a growing public health concern due to heightened human exposure to tick bites. The lone star tick (Amblyomma americanum: Ixodida: Ixodidae), a common human-biting species in the United States, has expanded its range in recent years. However, how its different life stages vary across time, habitats, and locations remains insufficiently understood. We analyzed tick abundance data collected in southeastern Virginia between 2009 and 2018, focusing on larval, nymphal, and adult life stages. A Bayesian multivariate modelling framework was used to examine seasonal patterns, habitat effects, spatial variation, and biological links between life stages. Two …

Evaluating Species At Risk In Data-Limited Fisheries: A Productivity-Susceptibility Analysis For Marine Aquarium Fish, Gabrielle A. Baillargeon, Alice A. Wynn, Jemelyn Grace P. Baldisimo, Michael F. Tlusty, Andrew L. Rhyne

Biological Sciences Faculty Publications

The marine aquarium trade (MAT) is a significant global industry harvesting millions of wild-caught, live coral reef fishes for public and private aquaria markets in the United States and Europe annually, while supporting fisher livelihoods in the Indo-Pacific. This diverse and species-rich trade is considered data-limited, creating barriers to quantifying the current and future socio-ecological sustainability of the fishery. We present a revised and expanded productivity–susceptibility analysis (PSA) that serves as a holistic risk assessment to estimate the vulnerability of marine aquarium fish to overfishing. Our global analysis includes 306 species that are actively in trade. Improvements to the PSA …

Does Fishery Management For Groupers (Teleostei: Epinephelidae) Protect Them Effectively? Context From The Iucn's Red List Of Threatened Species, Sean T. Fennessy, Christi Linardich, Kevin Rhodes, Joao P. Barreiros, David Pollard, Eloy Sosa-Cordero, Felicia Coleman, Alfonso Aguilar-Perera, Christopher R. Malinowski, Thierry Brulé, Pedro Afonso, Kayan Ma, Min Liu, Muktha Menon, Colin Wen, Stanley K. H. Shea, Sean N. Porter, Matthew Craig, Yvonne Sadovy De Mitcheson

Biological Sciences Faculty Publications

Worldwide, groupers (Epinephelidae) are commercially valued fishes, which also play key ecological roles on tropical and subtropical reefs. In 2007 and 2016, the IUCN's Groupers and Wrasses Specialist Group assessed all 160+ grouper species, with 17 of these being identified as threatened in 2016 and the major threat factor being overexploitation. Our present study aimed to identify whether management measures (MMs) for previously assessed groupers were established, whether these measures aligned with IUCN's Red List categories, and whether they effectively protect grouper populations. Experts in grouper biology and management assigned scores per grouper species based on the extent to which …

Three Decades Of Classifying Threatened Species: Lessons Learned From And About The Iucn Red List Criteria For Quantifying Extinction Risk, H. Resit Akçakaya, E. J. Milner-Gulland, Mike Hoffmann, Helen M. Regan, Ilona Naujokaitis-Lewis, Andre E. Punt, Kevin J. Gaston, David A. Keith, Stuart H. M. Butchart, Simon N. Stuart, Nigel J. Collar, Moreno Di Marco, Monika Bohm, Alex J. Berryman, David P. Mallon, Axel Hochkirch, Nicholas K. Dulvy, Malin Rivers, Thomas M. Brooks, James R. S. Westrip, Jonathan Paul Rodriguez, Craig Hilton-Taylor, Janet Scott, Simon Tarr, Sophie H. Ledger, Christi Linardich

Biological Sciences Faculty Publications

The IUCN Red List of Threatened Species, the most widely used global system for assessing species' extinction risk, has become a foundational source of information for conservation management, policy and research. Since the adoption of quantitative extinction risk criteria more than three decades ago, the Red List has expanded substantially in scope and influence, informing decisions ranging from species conservation and protected area designation to international agreements, corporate risk assessments and global biodiversity indicators. Given its central role, maintaining scientific rigour, transparency and trust in the Red List system is essential. Feedback from users, emerging from evolving applications and scientific …

The Coral Microbiome Under Climate Change: Shifting Symbiotic Interactions, Katherine E. Parker, Kate M. Quigley

Biological Sciences Faculty Publications

Host–microbe symbioses are central to organismal health, yet these complex partnerships are often strongly shaped by the environment. Climate change is increasingly altering environmental conditions, disrupting the balance of host–microbe interactions and pushing them along a continuum from mutualism (both partners benefit) toward parasitism (one partner gains at the other’s expense). Reef-building corals provide a clear example of this vulnerability, as the coral holobiont, an ecological unit comprising diverse photosynthetic algal symbionts (Symbiodiniaceae) and other microbial partners, depends largely on these mutualistic interactions for nutrition, immunity, and stress tolerance. Increasingly, evidence suggests that under heat stress, the algal symbionts retain …

[Review Of The Book A Conspectus Of The North American Isoetaceae, By D. F. Brunton, J. T. Kartesz, M. Nishino, And P. C. Sokoloff]., Lytton John Musselman

Biological Sciences Faculty Publications

Isoetes (Isoetaceae; Lycopodiopsida; quillworts) constitutes a highly successful, cosmopolitan genus with over 200 species. Isoetes is derived from isos Greek for equal and eter for year, referring to the typically long persisting leaves of the sole genus in this ancient plant family. The leaves resemble a ‘quill-of-feather,’ hence the common name ‘Quillworts,’ wort, from old Germanic wurti, meaning plant. In large measure due to its simple form, inconspicuous appearance and negligible economic value, it represents one of the least studied and least collected of all North American genera. Isoetes constitutes a taxonomically enigmatic group with numerous polyploid species that arose …

Mitochondrial Genome Of The Indo-Pacific Mesophotic Coral Leptoseris Columna (Scleractinia: Agariciidae) Assembled Using Pacbio Long-Read Sequencing, Nomita Rani Adhikary, Daniel J. Barshis, J. Antonio Baeza

Biological Sciences Faculty Publications

Leptoseris columna, a mesophotic coral species belonging to the family Agariciidae, is distributed throughout the Indo-Pacific region. This species is considered as of "Least concern" by the IUCN, yet, faces multiple local and global stressors. To support conservation plans, this study sequenced and characterized the complete mitochondrial genome of L. columna. The complete mitochondrial genome of Leptoseris columna was assembled using PacBio long-reads with a coverage of 459× per bp. The AT-rich mitochondrial genome of Leptoseris columna is 18,546 bp long and comprises 13 protein-coding genes (PCGs), 2 transfer RNA genes (trnM and trnW), and 2 ribosomal RNA …

Larval Genomics As A Viable, Fisheries-Independent Tool For Investigating Population Structure In Tropical Pacific Tunas, Jacob E. Jaskiel, Hannah E. Aichelman, Giulia Anderson, Louw Claassens, James E. Fifer, Christina M. Hernandez, Alexa K. Huzar, Joel K. Llopiz, Jan W. Witting, Sean P. Mullen, Randi D. Rotjan

Biological Sciences Faculty Publications

Understanding how dispersal, life history, and environmental variability shape genetic connectivity in the open ocean remains a central challenge in evolutionary biology. Highly migratory marine predators like tunas have traditionally been considered genetically homogeneous across ocean basins, yet emerging genomic evidence suggests that cryptic population structure can persist even in species with high gene flow and large effective population sizes. We used 2bRAD sequencing of 348 larval and subadult skipjack (Katsuwonus pelamis), yellowfin (Thunnus albacares), and bigeye tuna (T. obesus) collected from the central Pacific across 7 years of sampling to examine species boundaries, …

A Re-Evaluation Of Atlantic Bluefin Tuna (Thunnus Thynnus) Spawning Distribution In The Western Atlantic Ocean, David E. Richardson, Katrin E. Marancik, Malgorzata Adamus, Elizabeth A. Broughton, Robert K. Cowen, Trika Gerard, Christina M. Hernández, John J. Hoey, Joel K. Llopiz, Molly E. Lutcavage, Estrella Malca, Debra L. Palka, Lourdes Vásquez-Yeomans, Harvey J. Walsh, C. Michelle Willis, Glenn A. Zapfe

Biological Sciences Faculty Publications

There is currently a lack of consensus on the spatial, seasonal and decadal-scale patterns of Atlantic bluefin tuna (Thunnus thynnus) spawning in the western Atlantic Ocean. We compiled ichthyoplankton and reproductive data to characterize bluefin spawning patterns and the uncertainty in these patterns. Ichthyoplankton data indicates that bluefin spawn in the Gulf of America/Gulf of Mexico, northwest Caribbean Sea, north of the Bahamas, along the inshore edge of the Florida Current off the Carolinas and in the western Slope Sea. Reproductive studies identified these same spawning areas, as well as the Windward Passage and waters east of the …

Comparative Analysis Of Six New Chloroplast Genomes In Platanthera (Orchidaceae) Enhances Understanding Of Its Diversification, Lisa E. Wallace, Martin I. Batalla, Matthew Maisonave

Biological Sciences Faculty Publications

Platanthera is among the most diverse genera of orchids in north temperate regions, with high species diversity in Asia and North America. Despite many ecological studies in this group, little is known about its genomic diversity. Here, we report the newly annotated chloroplast genomes from six species in subgenus Limnorchis from North America and compare them with plastomes published for Platanthera species from Asia and Europe. We found that the plastomes of species in subg. Limnorchis are among the smallest reported for Platanthera thus far. While major structural differences were not detected in the newly sequenced species, ndh genes were …

Herbivore Identity And Species Richness Shape Fruit Chemical And Quality Responses To Foliar Herbivory, Xavier Ozowara, Taylor M. Sloey, Susan R. Whitehead

Biological Sciences Faculty Publications

The cascading consequences of foliar herbivory on fruit chemistry and quality remain relatively underexplored, with most existing studies focusing on the effects of only one or two attackers. In this study, we examine how increasing herbivore species richness during foliar herbivory alters strawberry (Fragaria × ananassa Duch) fruit quality and phenolic chemistry. We conducted a greenhouse experiment with single species feeding and different combinations of feeding by three generalist lepidopteran species: Spodoptera frugiperda, Heliothis virescens, Helicoverpa zea. Species fed on leaves for 72 h as fruits ripened, and fully ripe fruits were harvested 72 h after …

Tick Lipocalin Triggers Mammalian Igfbp-3-Mediated Apoptosis In Macrophages And Keratinocytes, Krittika Nandy, P. P. Mahesh, Lichao Liu, Daniel E. Sonenshine, Hameeda Sultana, Girish Neelakanta

Biological Sciences Faculty Publications

Ticks secrete several molecules, including lipocalins, in their saliva during blood feeding on a vertebrate host. In this study, we provide novel evidence on the role of Ornithodoros turicata americanus tick lipocalin (Otlip) in modulating cytokine expression and in triggering apoptosis in mammalian macrophages and keratinocytes. The cytokine array analysis revealed significantly increased secretion of insulin-like growth factor-binding protein 3 (IGFBP3) from murine macrophage cell line upon treatment with recombinant Otlip protein (rGST-Otlip) when compared to the secretion noted from cells treated with a control protein (rGST). Similar observation with cytokine protein array analysis was noted when murine macrophages were …

Microarray Analysis Of Human Abdominal Aortic Aneurysm With Emphasis On Cardiovascular Genes Revealed Differentially Expressed Genes, Song Lu, Li Ping Li, John V. White, Xiaoying Zhang, Ifeyinwa Nwaneshiudu, Adaobi Nwaneshiudu, Nectaria Ntaoula, John Gaughan, Dimitri S. Monos, Wan-Lu Lin, Charalambos C. Solomides, Emilia L. Oleszak, Chris D. Platsoucas

Biological Sciences Faculty Publications

Background/Aim: We examined gene expression profiles in abdominal aortic aneurysm (AAA) lesions vs. normal aortas by cDNA microarray and real-time quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR).

Materials and Methods: Phosphorus (32P)-labeled cDNA from AAA specimens (mean AAA size 6.65 cm) and normal aortas were hybridized with a 588-gene microarray primarily of the cardiovascular system. The results were validated by qRT-PCR.

Results: A total of 35 out of the 588 genes were differentially expressed, with either log2 ratio of AAAs/controls ≥1 (upregulated; 20 genes) or ≤−1 (downregulated; 15 genes) in AAA lesions vs. normal aorta, and 25 of these were significantly …

Challenging And Diagnosing Structured Population Models By Testing Predictions From Stochastic Demography, Stephen P. Ellner, Robin E. Snyder, Daniel T. Blumstein, Dylan Z. Childs, Joshua C. Fowler, Christina M. Hernández, Julien G. A. Martin, María Paniw, Yngvild Vindenes

Biological Sciences Faculty Publications

1. Structured population models are parameterized to accurately project expected population sizes, stage/state distributions and population growth rates, but they also predict the variation in outcomes among individuals, such as the variance and skewness of lifetime reproductive output (LRO) and lifespan, the probability of never reproducing, and many other life-history metrics.

2. Testing such predictions about individual outcomes can be very useful model 'stress tests', because they depend on how components of the model (e.g. submodels for survival and fecundity) interact over multiple time steps, not just on the accuracy of the submodels. Because data on among-individual variation is rarely …

Rapid Urbanization Reduces Genetic Diversity And Increases Genetic Differentiation Of A Lynx Spider Oxyopes Sertatus In Central Taiwan, Ying-Yuan Lo, Chi Wei, Wan-Jyun Chen, Chung-Ping Lin

Biological Sciences Faculty Publications

Urbanization is a dominant force driving destructive and irreversible changes of natural habitats in modern times. While the effects of urbanization on community composition and phenotypic responses are well-documented, its influence on genetic diversity and population structure remains understudied, particularly for invertebrates in subtropical regions. This study tested the hypothesis that urbanization reduces genetic diversity and increases population differentiation in the lynx spider Oxyopes sertatus, a common foliage-dwelling spider in Taiwan. We sampled 245 individuals from 17 sites distributed along an urban-rural gradient and quantified urbanization intensity using land-use composition at both landscape (4 km²) and local (0.25 km²) …

Differential Growth Dynamics Of Common Salt Marsh Species Spartina Alterniflora And Juncus Roemerianus Under Varied Sediment Amendments, Caitlin Hemphill, Richard P. Hale, Erik S. Yando

Biological Sciences Faculty Publications

Introduction

Many coastal wetlands are at high risk of degradation or loss due to sea‐level rise. Restoration techniques for maintaining coastal marshes are paramount, with thin‐layer placement (TLP) emerging as one feasible solution. Despite TLP's utilization, additional research is needed on species‐ and sediment‐specific responses.

Objectives

Our research combines greenhouse and field experimentation to test species and sediment composition responses aimed at maximizing vegetation while maintaining increased elevation.

Methods

The greenhouse experiment used two common marsh plants, Spartina alterniflora and Juncus roemarianus. Five centimeters of sediment (mud, sand, and sand/mud mix) was added, and subsequent sediment and vegetation metrics …

Freshwater Mussels Of Kansas: Register Of Taxa, Synonyms, And Assumed Misidentifications, Mark E. Eberle

Biological Sciences Faculty Publications

The literature on freshwater mussels (Mollusca: Margaritiferidae and Unionidae) of Kansas includes a collection of names of species and subspecies that makes it difficult for even experienced malacologists to decipher which taxa actually were collected. Compounding this problem is the absence of voucher specimens for some of the studies conducted in the state. This list was compiled to aid those who are conducting research and want to assess their results with reference to the earlier accounts. The alphabetical list of valid taxa, synonyms, and assumed misidentifications of freshwater mussels of Kansas is drawn from the literature and not an examination …

Developmental Regulation Of Corazonin, Eclosion Hormone, And Bursicon Messages And Rnai Suppression Of Corazonin In Adult Female American Dog Ticks, Dermacentor Variabilis, Anirudh Dhammi, Brooke Bissinger, Loganathan Ponnusamy, Daniel E. Sonenshine, R. Michael Roe

Biological Sciences Faculty Publications

The insect molting process is critical to growth and development and is regulated in part by the neuropeptides corazonin, eclosion hormone, and α and β bursicon. We found messages in a synganglion transcriptome from adult, female American dog ticks, Dermacentor variabilis (that do not molt), with a high similarity to the larval insect neuropeptides that control molting. The phylogenetic analysis of the tick putative neuropeptides compared to other arthropods is discussed in detail. The relative gene expression of these peptides was determined by quantitative PCR during the following adult developmental stages: (i) virgin, unfed 0–24 h after entering the adult …

Schistosoma Mansoni Infection Causes Consistent Changes To The Fecal Bacterial Microbiota Of Mice Across And Within Sites, Mariam A. Mhanna, David T. Gauthier, Lisa M. Shollenberger

Biological Sciences Faculty Publications

Eggs of Schistosoma mansoni are produced by adult female worms in mesenteries of infected hosts. Eggs can cross the intestinal barrier and form granulomas in the tissue or breach and exit the host through fecal excretion. These interactions may affect the host microbiome assemblages. Given the potential for schistosomal alteration of host gut microbiome and subsequent effects on the fecal bacterial composition, it is important to conduct controlled microbiome studies on model animals. While pursuing these studies, it is important to take into account the different conditions in which microbiome studies are conducted and their consequent impacts on variability and …

The First Iucn Red List Of Cold-Water Corals Highlights Global Declines, Julia D. Sigwart, A. Louise Allcock, Renata Carolina Mikosz Arantes, Kelsey Archer Barnhill, Narissa Bax, Julia S. Beneti, Saskia Brix, Gudmundur Gudmundsson, Catherine S. Mcfadden, Severin A. Korfhage, Christi Linardich, Declan Morrissey, Pedro De Oliveira Nascimento, Bárbara De Moura Neves, Steinunn H. Ólafsdóttir, Stefán Ragnarsson, Kaveh Samimi-Namin, Íris Sampaio, Mark E. De Wilt

Biological Sciences Faculty Publications

The most well-known species-based conservation tool is the International Union for the Conservation of Nature (IUCN) Red List of Threatened Species. The current coverage of species in the Red List is known to under-represent benthic marine species. Cold-water corals (CWCs) are increasingly recognised as key to deep-water biodiversity and integral to protected vulnerable marine ecosystems (VMEs), but no deep-sea coral species were previously included in the Red List. We selected 22 cold-water coral species in the Northeast Atlantic, including 4 reef-forming stony corals and 18 octocorals including sea pens and gorgonians, and completed the first IUCN Red List global assessments …

A Blueprint To Greener Shorelines: Advancing The Effectiveness, Sustainability, And Widespread Adoption Of Coastal Nature-Based Solutions Through Transdisciplinary Research, Taylor M. Sloey, Sierra Hildebrandt, Rebecca L. Morris, Matthew V. Bilskie, Aaron Bland, David Bushek, Gabriella Dipetto, Daniel Elefant, Vincent Encomio, Ramin Familkhalili, Christine Hladik, Danielle Kreeger, Avery B. Paxton, Cindy M. Palinkas, Latina Steele, Andrew Scheld, Daisuke Taira, Jason D. Toft, Armando J. Ubeda, Christine Whitcraft, Donna Marie Bilkovic

Biological Sciences Faculty Publications

Coastal nature-based solutions (NbS) have emerged as powerful tools to enhance sustainable development and ecological restoration goals. As a rapidly growing field spanning across social, political, ecological, economic, and engineering disciplines, it is critical that researchers working in coastal NbS regularly attempt to identify emerging focal areas for scientific inquiry. Following the 27th Biennial meeting of the Coastal and Estuarine Research Federation, we provide a transdisciplinary perspective (including biologists, engineers, oceanographers, geoscientists, economists, and facilitators of workforce training programs) of pertinent research questions that, if answered, will advance the effectiveness, sustainability, and widespread adoption of coastal NbS. These suggestions for …

Thermal Tolerance Limits Of Mesophotic Corals In American Samoa, Daniel Barshis, Veronica Z. Radice, Katherine E. Parker, Nicholas Evensen

Biological Sciences Faculty Publications

We analyzed the thermal tolerances of multiple coral species from an upper mesophotic (38–40m) and a shallow reef (5–10m) with our portable Coral Bleaching Automated Stress System (CBASS). Depth generalist Montipora grisea (deep and shallow) and depth specialists Pachyseris speciosa and Leptoseris sp. (deep only) were assayed from Leone, located on the western coast of Tutuila in American Samoa. Fragments from 10 genotypes of each species were exposed to a 17-hour thermal stress assay reaching four maximum temperatures ranging from 30–38°C under in situ light conditions (150 μmol photons m⁻² s⁻¹ under a Lee Filters “deep …

Form And Function In Mobulids: A Comparative Analysis Of Filter Morphology With Bioinspiration Applications, J. B. Teeple, S. R. Kahane-Rapport, K. E. Cohen, L. Hamann, J. A. Strother, E. W. M. Paig-Tran

Biological Sciences Faculty Publications

Mobulas (manta and devil rays) are large-scale ram filter feeders that separate planktonic food particles from large volumes of water with minimal clogging. This contrasts with most human-made filters that can suffer from problematic clogging requiring additional mechanisms for clearing blocked surfaces and maintaining performance. Prior studies have shown that mobulas employ a unique mechanism referred to as ricochet separation to filter feed, whereby captive vortices in filter pores cause particles to bounce off the filter surfaces and away from the filter pores. This mechanism enables the filtration of particles smaller than the pore size and reduced clogging. However, few …

Carbon Dynamics Across The Mangrove-Tidal Flat Ecotone In A Tropical Seascape, E. S. Yando, I. J.B. Alemu, L. Kiah Eng, T. M. Sloey, M. Van Breugel, Bhatia N. Friess

Biological Sciences Faculty Publications

Mangroves and other blue carbon ecosystems are important for climate through the storage and sequestration of carbon. Most research estimates carbon dynamics by subsampling central portions of the ecosystem and are then upscales using habitat maps to patch, landscape, or regional levels. However, heterogeneity is rarely accounted for - particularly at edges. Further, adjacent tidal flat ecosystems have only recently been considered for carbon storage, but may be undervalued if areas where they transition with vegetated systems are not accounted for. To address the lack of information at coastal ecotones, we examined both soil carbon storage and carbon dioxide flux …

Caveolin-Mediated Endocytosis: Bacterial Pathogen Exploitation And Host-Pathogen Interaction, Dibyasri Barman, Rishi Drolia

Biological Sciences Faculty Publications

Within mammalian cells, diverse endocytic mechanisms, including phagocytosis, pinocytosis, and receptor-mediated endocytosis, serve as gateways exploited by many bacterial pathogens and toxins. Among these, caveolae-mediated endocytosis is characterized by lipid-rich caveolae and dimeric caveolin proteins. Caveolae are specialized microdomains on cell surfaces that impact cell signaling. Caveolin proteins facilitate the creation of caveolae and have three members in vertebrates: caveolin-1, caveolin-2, and caveolin-3. Many bacterial pathogens hijack caveolin machinery to invade host cells. For example, the Gram-positive facultative model intracellular bacterial pathogen Listeria monocytogenes exploits caveolin-mediated endocytosis for efficient cellular entry, translocation across the intestinal barrier, and cell–cell spread. Caveolin …

Lap-Hsp60 Complex Modulates Epithelial Tight Junction Barrier, Arun Bhunia, Manalee Manalee Samaddar, Chen Sun, Nicholas Gallina, Nicole Irizarry-Tardi, Donqi Liu, Shivendra Tenguria, Rishi Drolia, Anika Jain, Daisuke Kihara, Wen Jiang, Kee-Hong Kim, Abigail Cox, Kurt Ristroph, Gregory Knipp, Nicholas Noinaj

Biological Sciences Faculty Publications

During infection, Listeria adhesion protein (LAP), a housekeeping enzyme, acts as a tight junction modulator (TJM) through interaction with Hsp60 to facilitate Listeria monocytogenes translocation across the intestinal epithelial barrier. Here, we used purified LAP as a potential TJM to overcome the limiting and variable effects observed by other agents in the class. We structurally determined the LAP interaction alone and in complex with Hsp60 utilizing cryo-EM and computational analysis. LAP structure resolved at 2.83 Å, forms multimeric interlocking dimers and tetramers, and the N-domain interacts with Hsp60, while the C-domain bridges the bacterial surface receptor InlB. The structural studies …

Low Salinities Impair Heat Tolerance And Alter Reproductive Traits With No Implications For Survival In A Marine Annelid From Coastal Habitats, Gabrielle Newton, Gloria Massamba-N'Siala

Biological Sciences Faculty Publications

Climate change is expected to intensify salinity and temperature fluctuations in estuarine and coastal habitats. However, the impact of salinity variation on ectotherms’ thermal tolerance remains underexplored, limiting our understanding of the interplay between these stressors. This study investigated salinity-dependent changes in life history traits (survival, growth rate, fecundity, and egg size) over a 33-day exposure to a salinity gradient (12, 17, 22, 27, 32, 35, 40, 45, 50) in the marine annelid Ophryotrocha labronica. Individuals that survived the salinity treatments were assessed for their upper and lower thermal tolerance limits. Female individuals, the target of the study, exhibited …

Cellular Responses Of Astrangia Poculata (Ellis And Solander, 1786) And Its Symbiont To Experimental Heat Stress, Tyler E. Harman, Daniel Barshis, Briana Hauff Salas, Kevin B. Strychar

Biological Sciences Faculty Publications

Climate change has had devastating effects on tropical coral reefs; however, much less is known regarding how heat stress affects temperate coral. This research focuses on Astrangia poculata (Ellis and Solander, 1786) collected from Narragansett Bay, RI, during the summer and winter seasons and understanding the effect of experimental thermal extremes (i.e., 26 °C) on seasonally different populations. Photosynthetic efficiency (Fv/Fm), symbiont density (via an inverse relationship with pixel intensity), and oxidative stress via reactive oxygen species (ROS) concentrations were measured on symbiotic and aposymbiotic A. poculata. Higher Fv/Fm rates were observed in summer- vs. winter-collected corals (p ≤ …