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Limnological Assistance For Lake Mead National Recreation Area: Quarterly Report, Period Ending December 31, 2011, Margaret N. Rees

Limnological Studies

Project 1

• Technical assistance with the implementation of the Interagency Monitoring Action Plan (IMAP) for Quagga Mussels is ongoing; remaining analyses are underway with report delivery rescheduled for 05/2012.
• Data have been delivered for Veliger abundance and settlement at different depths of Lake Mead with report delivery re-scheduled for 05/2012.
• Growth of adult quagga mussels within Las Vegas Wash/Las Vegas Bay
• The Interagency Quagga Mussel Meeting was held on 11/17/2011; the next meeting is scheduled for 02/16/2011.

Project 2

• Appendix 7, an addition to the Long-term Limnological and Aquatic Resource Monitoring and Research Plan for Lakes Mead and Mohave, has …

Characterisation Of Rapeseed Oil Based Resins Using Infrared And Thermogravimetry Techniques, L. Y. Mwaikambo

Tanzania Journal of Science

Rapeseed oil which is a triglyceride had undergone chemical modification on its structure to obtain hydroxyl groups. The hydroxylated chemical structure was crosslinked using methylene-pphenyl diisocyanate to produce a thermoset rapeseed oil resin. The cross linking process was monitored in situ using the Attenuated Total Internal Reflectance Fourier Transform-Infrared spectroscopy and the thermogravimetric analysis techniques. The infrared technique was also used to monitor chemical groups of the unmodified rapeseed oil. A reduction of the absorption band around 3300 cm-1 which is due to the presence of hydroxyl groups was observed indicating the occurrence of the reaction between the hydroxylated rapeseed …

Landsat Remote Sensing Data As An Alternative Approach For Geological Mapping In Tanzania: A Case Study In The Rungwe Volcanic Province, South-Western Tanzania, E. E. Mshiu

Tanzania Journal of Science

Rungwe Volcanic Province (RVP) is mostly covered by extrusive rocks that overlain the Precambrian basement. The use of Landsat data in this area has revealed the need of effective use of these data in geological mapping programs in Tanzania. Landsat band ratios 5/1, 3/7, 5/7 and 5/4 as well as R: G: B composite images 7:4:1, 7:5:4, 1/3:5/7:3/5 and 4/5:6/7:4/6 played an important role in identifying different rock types in the study area. Ratio images managed clearly to distinguish between mafic and felsic rocks whereby two lithological blocks were identified, Block 1 covers a combination of intrusive and metamorphic rocks …

Growth Performance, Yields And Economic Benefits Of Nile Tilapia Oreochromis Niloticus And Kales Brassica Oleracea Cultured Under Vegetable-Fish Culture Integration, A. P. Shoko, A. Getabu, G. Mwayuli, Y. D. Mgaya

Tanzania Journal of Science

An experiment was conducted for 210 days to demonstrate the role of vegetable-fish culture integration in the growth, yields and economic benefits of fish and vegetables. Two 200 m2 earthen fishponds were stocked with Nile tilapia Oreochromis niloticus at 20,000 fish fingerlings per hectare. Pond A was fertilized with chicken manure and stocked fish fed on 35% crude protein supplementary diet referred to here as treated fish pond (TFP). Another fish pond was not fertilized and the fish stocked in it did not receive any supplementary diet referred to here as non treated fish pond (NTFP). Twelve vegetable plots of …

Variability In Parasites’ Community Structure And Composition In Cat Fish With Respect To Level Of Pollution In Mwanza Gulf, Lake Victoria, Tanzania, C. J. Mwita

Tanzania Journal of Science

This study investigated the composition and structure of the parasite communities in Cat fish with respect to levels of water pollution in Lake Victoria. A total of 1071 Clarias gariepinus with mean TL range of 19 to 27 cm were analyzed from three localities in Mwanza Gulf (Kirumba, 298 fish infected with 15 parasite species), Nyegezi (376 fish, with 15 parasites species) and Malimbe (397 fish, with 16 parasites species). Trematodes and nematodes dominated the population with six (35.3%) and five (29.4%) species, respectively. Mean species richness and heteroxenous/monoxenous ratio showed significant changes (Fr = 8.000; p = 0.0046), being …

Host-Parasites Relationship For Lake Victoria Clariid Fishes And Their Parasite Fauna, C. J. Mwita

Tanzania Journal of Science

Host-parasite co-evolutionary relationships for the clariid fish species of the Lake Victoria basin and their parasite fauna was inferred by cytochrome b gene and the 18S rDNA using ‘a strict coevolutionary’ constrained tree method, the results of which revealed a phylogenetic incongruence between the fish hosts and their parasite fauna. The present study further indicated that the sympatric association between the clariid hosts and non-clariid fishes in Lake Victoria makes it possible for the parasites to switch from one host to another and hence the large number of shared parasites observed. The specificity of some of the parasites, e.g. Diplostomum …

Anthropogenic Disturbance On The Vegetation In Makurunge Woodland, Bagamoyo District, Tanzania, C. Mligo, C. Mligo

Tanzania Journal of Science

Makurunge woodland is part of the major vegetation component covering coastal forest landscape in Tanzania that has been severely affected by anthropogenic disturbance. The present study determined the effects of anthropogenic disturbance on biomass, diversity, plant communities and plant species distribution pattern using nested sample plots systematically established along transects. Six major vegetation communities with different biomass were found. Plant species diversity ranged from 2.5 to 3.3 and was high in scrub forests, riverine and thicketed habitats, although the difference among these habitats was not significant (P>0.05). About 61-90% of the woodland was affected and this influenced the plant …

Classification Of Plants According To Their Heavy Metal Content Around North Mara Gold Mine, Tanzania: Implication For Phytoremediation, N. Mganga, Mlk Manoko, Z. K. Rulangaranga

Tanzania Journal of Science

Plants like other living organisms respond differently under different environmental conditions. An elevated level of heavy metals is one of the stresses which results into three classes of plants depending on their heavy metal content. The classes of plant species according to their accumulated heavy metals around North Mara Gold Mine were not known. To study such classes, a line transect of 700m long was established opposite the gold mine wastes. A total of eight sampling points were systematically established each after every 100m in that transect. Fifteen plant species were sampled; at least one species per sampling point. Approximately …

Evaluation Of Single-Step Steam Pyrolysis-Activated Carbons From Lesotho Agro-Forestry Residues, I. Hapazari, V. Ntuli, W. Parawira

Tanzania Journal of Science

Activated carbon has been widely used worldwide as an effective filtration or adsorption material for removing biological and chemical contaminants from drinking water. The potential of producing activated carbon (AC) from local agroforestry residues by single-step steam pyrolysis processes was investigated. The research aims to promote innovative use of local raw materials and relatively low-cost techniques in production of activated carbon. Four varieties of activated carbons from four different agroforestry residues, pine (Pinus contorta) cones (PC), Abies (Abies cilicica) seeds (AS), maple (Acer ginnala) seeds (MS) and peach (Prunus persica) stones (PS), were prepared and evaluated. The ACs were evaluated …

Spt5 Affects The Rate Of Mrna Degradation And Physically Interacts With Ccr4 But Does Not Control Mrna Deadenylation, Yajun Cui, Yueh-Chin Chiang, Palaniswamy Viswanathan, Darren J. Lee, Clyde L. Denis

New Hampshire Agricultural Experiment Station Publications

The CCR4-NOT complex has been shown to have multiple roles in mRNA metabolism, including that of transcriptional elongation, mRNA transport, and nuclear exosome function, but the primary function of CCR4 and CAF1 is in the deadenylation and degradation of cytoplasmic mRNA. As previous genetic analysis supported an interaction between SPT5, known to be involved in transcriptional elongation, and that of CCR4, the physical association of SPT5 with CCR4 was examined. A two-hybrid screen utilizing the deadenylase domain of CCR4 as a bait identified SPT5 as a potential interacting protein. SPT5 at its physiological concentration was shown to immunoprecipitate CCR4 and …

Two Epiparasitic Species Of Phoradendron (Viscaceae) From Honduras: One New And For The Other A Range Extension And Host Determination, Delbert Wiens, Clyde L. Calvin

Aliso: A Journal of Systematic and Floristic Botany

A new species of Phoradendron, Ph. mathiasenii, is described, and a range extension and host are given for Ph. tikalense. Both species are from Honduras and grow as epiparasites, i.e., mistletoes parasitizing other mistletoes. The parasitic host for both epiparasitic species of Phoradendron is Psittacanthus angustifolius (Loranthaceae), which in turn is parasitic on the terrestrial host, Pinus oocarpa, a common forest tree in the collection area. Although the two species of Phoradendron parasitize the same parasitic host and co-occur in the area, they are distinct morphologically. Many plants of Ph. mathiasenii were observed at several collection …

Cover Page

Aliso: A Journal of Systematic and Floristic Botany

No abstract provided.

A New Variety Of Lomatium Ravenii (Apiaceae) From The Northern Great Basin And Adjacent Owyhee Region, Kimberly M. Carlson, Donald H. Mansfield, James F. Smith

Aliso: A Journal of Systematic and Floristic Botany

Variability in the group of Lomatium species comprising L. nevadense, L. ravenii, and L. foeniculaceum has led to conflicting classification schemes. While some taxonomists have treated L. ravenii as a distinct species made up of all the populations from California, Nevada, Idaho, and Oregon, others considered L. ravenii to be nothing more than a morphological extreme of L. nevadense. We examined morphological and phylogenetic data from across the range of L. ravenii, concluding that variation in the species warrants varietal distinction. Non-metric multidimensional scaling analysis of 29 populations shows two distinct groups—one from the vicinity of …

Rediscovery Of Monotropastrum Sciaphilum (Andres) G.D.Wallace In China After 91 Years, Min Shen, Chang-Qin Zhang, Gary D. Wallace

Aliso: A Journal of Systematic and Floristic Botany

Monotropastrum sciaphilum (Ericaceae), a mycoheterotrophic member of subfamily Monotropoideae, was rediscovered at its type locality in Yunnan Province, China, 91 years after it was first collected. The type locality is the only locality from which it is known. Field observations in 2007–2010 indicated that inflorescences emerge from the soil between late April and early September. The restricted distribution of M. sciaphilum recommends its listing in the IUCN red book.

Index

Aliso: A Journal of Systematic and Floristic Botany

No abstract provided.

The Flora Of Greater San Quintín, Baja California, Mexico (2005–2010), Sula E. Vanderplank

Aliso: A Journal of Systematic and Floristic Botany

The plants of San Quintín (Baja California, Mexico) were documented through intensive fieldwork and collection of herbarium specimens to create a checklist of species. The region is located near the southernmost extent of the California Floristic Province, and the flora is influenced by the adjacent desert to the south. A total of 435 plant taxa were documented, of which 82% are native plants. Almost one in three native plant taxa in Greater San Quintín is a sensitive species, here documented as rare and/or locally endemic, and many taxa have yet to be evaluated fully for rarity and threats. Four major …

Enhanced Microbial Utilization Of Recalcitrant Cellulose By An Ex Vivo Cellulosome-Microbe Complex, Chun You, Xiao-Zhou Zhang, Noppadon Sathitsuksanoh, Lee R. Lynd

Dartmouth Scholarship

A cellulosome-microbe complex was assembled ex vivo on the surface of Bacillus subtilis displaying a miniscaffoldin that can bind with three dockerin-containing cellulase components: the endoglucanase Cel5, the processive endoglucanase Cel9, and the cellobiohydrolase Cel48. The hydrolysis performances of the synthetic cellulosome bound to living cells, the synthetic cellulosome, a noncomplexed cellulase mixture with the same catalytic components, and a commercial fungal enzyme mixture were investigated on low-accessibility recalcitrant Avicel and high accessibility regenerated amorphous cellulose (RAC). The cellbound cellulosome exhibited 4.5- and 2.3-fold-higher hydrolysis ability than cell-free cellulosome on Avicel and RAC, respectively. The cellulosome-microbe synergy was not completely …

Natural Variation In Gestational Cortisol Is Associated With Patterns Of Growth In Marmoset Monkeys (Callithrix Geoffroyi), Aaryn C. Mustoe, Andrew K. Birnie, Andrew V. Korgan, Jonathan Bruce Santo, Jeffrey French

Psychology Faculty Publications

High levels of prenatal cortisol have been previously reported to retard fetal growth. Although cortisol plays a pivotal role in prenatal maturation, heightened exposure to cortisol can result in lower body weights at birth, which have been shown to be associated with adult diseases like hypertension and cardiovascular disease. This study examines the relationship between natural variation in gestational cortisol and fetal and postnatal growth in marmoset monkeys. Urinary samples obtained during the mother’s gestation were analyzed for cortisol. Marmoset body mass index (BMI) was measured from birth through 540 days in 30- or 60-day intervals. Multi-level modeling was used …

Transcriptomic Profiles Of Peripheral White Blood Cells In Type Ii Diabetes And Racial Differences In Expression Profiles, Jinghe Mao, Junmei Ai, Xinchun Zhou, Ming Shenwu, Manuel Ong Jr., Marketta Blue, Jasmine T. Washington, Xiaonan Wang, Youping Deng

Faculty Publications

Background: Along with obesity, physical inactivity, and family history of metabolic disorders, African American ethnicity is a risk factor for type 2 diabetes (T2D) in the United States. However, little is known about the differences in gene expression and transcriptomic profiles of blood in T2D between African Americans (AA) and Caucasians (CAU), and microarray analysis of peripheral white blood cells (WBCs) from these two ethnic groups will facilitate our understanding of the underlying molecular mechanism in T2D and identify genetic biomarkers responsible for the disparities.

Results: A whole human genome oligomicroarray of peripheral WBCs was performed on 144 …

Pentachlorophenol Decreases Tumor-Cell-Binding Capacity And Cell-Surface Protein Expression Of Human Natural Killer Cells, Tasia Hurd, Jasmine Walker, Margaret M. Whalen

Chemistry Faculty Research

Pentachlorophenol (PCP) is an organochlorine pesticide that decreases the tumor-cell killing (lytic) function of human natural killer (NK) cells. NK cells defend against tumor cells and virally infected cells. They bind to these targets, utilizing a variety of cell-surface proteins. This study examined concentrations of PCP that decrease lytic function for alteration of NK binding to tumor targets. Levels of PCP that caused loss of binding function were then examined for effects on expression of cell-surface proteins needed for binding. Exposure to 10 µm PCP for 24 h (which caused a greater than 70% loss of lytic function) decreased NK …

Development Of An Environmentally Friendly Binder System For Paper Products, Ashley Rhea Flory

Student Theses and Dissertations

It is important for industries to find ways to find green chemistries to protect the environment and the paper industry is no exception. Using renewable resources such as plant material will be an excellent substitute for the chemicals that are currently used as paper binders. In this project, carbohydrates, proteins and phenolic compounds were applied to air laid, pressed paper in order to obtain a binder that is as strong as the currently used factory binders while being organic and renewable. Out of the twenty binders surveyed soy protein, gelatin, zein protein, pectin and Salix lignin provided comparable strength results …

Children's Knowledge Of Healthy And Unhealthy Foods And Its Correlation With Body Mass Index, Sally Elizabeth Allen

Student Theses and Dissertations

Childhood obesity is a growing epidemic in America. Children's knowledge of healthy and unhealthy food could be correlated to their Body Mass Index (BMI). Therefore, the purpose of this study is to determine if there is a correlation between children's BMI and their knowledge of healthy and unhealthy foods and to determine if any knowledge differences exist between overweight, obese, and healthy weight children. Participants were 54 kindergarten and 1st grade students in a small rural Arkansas school district. Data were collected quantitatively through a) a 40 question flashcard test in which participants were asked to identify the name of …

A Shared Gene Expression Signature In Mouse Models Of Ebv-Associated And Non-Ebv-Associated Burkitt Lymphoma, Kathryn T. Bieging, Kamonwan Fish, Subbarao Bondada, Richard Longnecker

Microbiology, Immunology, and Molecular Genetics Faculty Publications

The link between EBV infection and Burkitt lymphoma (BL) is strong, but the mechanism underlying that link has been elusive. We have developed a mouse model for EBV-associated BL in which LMP2A, an EBV latency protein, and MYC are expressed in B cells. Our model has demonstrated the ability of LMP2A to accelerate tumor onset, increase spleen size, and bypass p53 inactivation. Here we describe the results of total gene expression analysis of tumor and pretumor B cells from our transgenic mouse model. Although we see many phenotypic differences and changes in gene expression in pretumor B cells, the transcriptional …

Morpholino Gene Knockdown In Adult Fundulus Heteroclitus: Role Of Sgk1 In Seawater Acclimation, Emily G. Notch, Joseph R. Shaw, Bonita A. Coutermarsh, Marisa Dzioba, Bruce A. Stanton

Dartmouth Scholarship

The Atlantic killifish (Fundulus heteroclitus) is an environmental sentinel organism used extensively for studies on environmental toxicants and salt (NaCl) homeostasis. Previous research in our laboratory has shown that rapid acclimation of killifish to seawater is mediated by trafficking of CFTR chloride channels from intracellular vesicles to the plasma membrane in the opercular membrane within the first hour in seawater, which enhances chloride secretion into seawater, thereby contributing to salt homeostasis. Acute transition to seawater is also marked by an increase in both mRNA and protein levels of serum glucocorticoid kinase 1 (SGK1) within 15 minutes of transfer. …

High Connectivity In The Deepwater Snapper Pristipomoides Filamentosus (Lutjanidae) Across The Indo-Pacific With Isolation Of The Hawaiian Archipelago, Michelle R. Gaither, Shelley A. Jones, Christopher Kelley, Stephen J. Newman, Laurie Sorenson, Brian W. Bowen

Fisheries Research Articles

In the tropical Indo-Pacific, most phylogeographic studies have focused on the shallow-water taxa that inhabit reefs to approximately 30 m depth. Little is known about the large predatory fishes, primarily snappers (subfamily Etelinae) and groupers (subfamily Epinephelinae) that occur at 100–400 m. These long-lived, slow-growing species support fisheries across the Indo-Pacific, yet no comprehensive genetic surveys within this group have been conducted. Here we contribute the first range-wide survey of a deepwater Indo-Pacific snapper, Pristipomoides filamentosus, with special focus on Hawai'i. We applied mtDNA cytochrome b and 11 microsatellite loci to 26 samples (N = 1,222) collected across …

Reverse Protection Assay: A Tool To Analyze Transcriptional Rates From Individual Promoters, Yan O. Zubo, Victor V. Kusnetsov, Thomas Börner, Karsten Liere

Dartmouth Scholarship

Transcriptional activity of entire genes in chloroplasts is usually assayed by run-on analyses. To determine not only the overall intensity of transcription of a gene, but also the rate of transcription from a particular promoter, we created the Reverse RNase Protection Assay (RePro): in-organello run-on transcription coupled to RNase protection to define distinct transcript ends during transcription. We demonstrate successful application of RePro in plastid promoter analysis and transcript 3' end processing.

Design And Synthesis Of Cationic Steroid Antimicrobial Compounds, Synthesis Of Glycolipids Recognized By Natural Killer T Cells And Development Of Tlr-1, Tlr-6 Heterodimer Binders And Studies Of Their Immunology Activities, Yanshu Feng

Theses and Dissertations

Cationic steroid antimicrobial agents (CSAs) are a family of bile acid derivatives. These compounds are amphiphilic and mimic endogenous antimicrobial peptides. The antimicrobial activities of CSA-13 have been investigated and due to portent bactericidal activities and low toxicity, a large amount of CSA-13 is demanded for clinic trails and other antimicrobial applications. During our studies, we optimized the synthetic route of CSA-13, so that it can be prepared at the kilogram, even in tons scale. We investigated three routes and one of them is suitable for industry, because only recrystallization is needed in the synthesis. Natural killer T cells (NKT …

The Murine Caecal Microrna Signature Depends On The Presence Of The Endogenous Microbiota, Natasha Singh, Elize A. Shirdel, Levi Waldron, Regan-Heng Zhang, Igor Jurisica, Elena M. Comelli

Publications and Research

The intestinal messenger RNA expression signature is affected by the presence and compo-sition of the endogenous microbiota, with effects on host physiology. The intestine is also characterized by a distinctive micronome. However, it is not known if microbes also impact intestinal gene expression epigenetically. We investigated if the murine caecal microRNA expression signature depends on the presence of the microbiota, and the potential implica-tions of this interaction on intestinal barrier function. Three hundred and thirty four mi-croRNAs were detectable in the caecum of germ-free and conventional male mice and 16 were differentially expressed, with samples from the two groups clustering …

Design Of A Tetracycline Operon Inducible System For The Control Of Vaccinia Virus Replication: Implications For Vaccine Development, Caitlin J. Hagen

Master's Theses

The use of vaccinia virus (VACV) as a vaccine resulted in the eradication of smallpox in 1979. Characteristics that contribute to the effectiveness of VACV as a vaccine and viral vector include its ability to elicit strong, long-lived humoral and cell-mediated immune responses as a live-replicating virus and to accept large inserts of DNA into its genome. However, adverse events associated with its use as the smallpox vaccine have constrained it from being more widely utilized in vaccines and therapies. We propose to improve the safety of VACV as a live-replicating vector by using elements of the tet operon to …

Psychometric Evaluation Of The Timeline Followback For Exercise Among Young Adults, Gregory A. Panza

Master's Theses

Objectives: Two separate studies assessed psychometric properties of a retrospective behavioral measure adapted for exercise called the Timeline Followback for Exercise (TLFB-E). Study one examined criterion, convergent, and predictive validity. Study two examined test-retest reliability. Methods: Study one participants (N = 66) were college students 20.0 ± 1.4yr. Validity of frequency, intensity, time, and type of exercise as assessed on the TLFB-E was examined using Pearson r correlations with accelerometers, weekly exercise contracts between participants and researchers, College Alumni Questionnaire, and a health-related physical fitness battery. Study two participants were a separate sample (N = 40) of college …