Life Sciences Commons^™

Crowdfunding For Biotechs: How The Sec’S Proposed Rule May Undermine Capital Formation For Startups, Brian J. Farnkoff

Journal of Contemporary Health Law & Policy (1985-2015)

No abstract provided.

U.S. Drought Monitor, December 31, 2013, Matthew Rosencrans

United States Agricultural Commodities in Drought Archive

Drought map of U.S. for December 31, 2013 (12/31/13) plus: U.S. crop areas experiencing drought (map), Approximate percentage of crop located in drought, by state (bar graph), Percent of crop area located in drought, past 52 weeks (line graph) for: Corn, Soybeans, Hay, Cattle, Winter wheat.

Partitioning The Fitness Components Of Rna Populations Evolving In Vitro, Carolina Diaz Arenas, Niles Lehman

Chemistry Faculty Publications and Presentations

All individuals in an evolving population compete for resources, and their performance is measured by a fitness metric. The performance of the individuals is relative to their abilities and to the biotic surroundings – the conditions under which they are competing – and involves many components. Molecules evolving in a test tube can also face complex environments and dynamics, and their fitnessmeasurements should reflect the complexity of various contributing factors as well. Here, the fitnesses of a set of ligase ribozymes evolved by the continuous in vitroevolution system were measured. During these evolution cycles there are three different catalytic …

The Processing Of Amyloid Precursor Protein In The Central Nervous System Of Humans And Rhesus Macaques, Justyna Anna Dobrowolska

All Theses and Dissertations (ETDs)

Alzheimer's disease: AD) is the most common dementia, currently afflicting 30 million people worldwide, with prevalence steadily increasing. Over 99% of AD cases are classified as sporadic, in which the major risk factors are age: greater than 65 years) and the ApoE-ε4 allele: in a gene dose dependent manner). The minority of AD cases: less than 1%) are caused by autosomal dominant inheritance of a genetic mutation in one of three genes: Amyloid Precursor Protein: APP), Presenilin-1: PSEN1), or Presenilin-2: PSEN2). Mutation carriers will generally notice cognitive decline starting at a relatively young age, anytime between their 30s-50s, and eventually …

The Development Of Fisheries Management In Liberia : Vessel Monitoring System (Vms) As Enforcement And Surveillance Tools : National And Regional Perspectives, Sheck Abdul Sherif

World Maritime University Dissertations

No abstract provided.

The Effects Of Nesting Environment On Neonatal Hypoxic-Ischemic Injury, Laura Grace Rollins

Graduate Masters Theses

Term neonates with hypoxic-ischemic (HI) injury are at risk for devastating neurological sequelae. The objective of this study is to determine if altering the early environment for maternal care-taking impacts the immediate and long-term sequelae of HI offspring. The Rice-Vannucci model was used to induce HI in postnatal day (PND) 7 Long-Evans pups. Litters were assigned to a closed nest (CN) or normal standard housing (SH) condition. Neurobehavioral development, cognitive ability, and stress response were assessed to establish any benefits of the CN condition. Finally, postmortem brain tissue was analyzed for morphometric markers of injury.

The Regulation Of Prospore Membrane Shape In Saccharomyces Cerevisiae, Emily M. Parodi

Graduate Doctoral Dissertations

The size and shape of a membrane is an important determinant in cell morphology. My work focuses on how membrane size and shape is determined, using the budding yeast S. cerevisiae as a model. During sporulation in S. cerevisiae, the diploid cell remodels its interior such that four spores are formed within an ascus. During this remodeling process, the prospore membrane is synthesized de novo and grows to surround each of the meiotic products. The prospore membrane is a double lipid bilayer and ultimately determines the sizes and shapes of the newly formed spores. My work focuses on the …

2013 Oregon Wine Harvest Report, Oregon Wine Board

Oregon Wine Board Documents

This harvest report for the Oregon wine industry from 2013 describes the season as "a play in two acts, with a wet intermission." Vintners were surprised by record rainfall one weekend in late September after a warm and dry summer. The rains, remnants from a typhoon in Japan, mostly affected the Willamette Valley, but effects were variable even within that region; southern and eastern Oregon were less affected by the storm.

Smokejumper Obituary: Foresman, James Buckey "Jim" (Mccall 1957), National Smokejumper Association

Smokejumper Obituaries

No abstract provided.

The Osh Family Of Proteins And Their Role In Caloric Restriction-Mediated Lifespan Extension In The Budding Yeast Saccharomyces Cerevisiae, William Douglas Craft

Theses and Dissertations

Oxysterol-binding protein homolouges (OSH) have been implicated in mediating non-vesicular sterol transport. Caloric restriction (CR) down-regulates sterol synthesis in a variety of organisms, including the budding yeast Saccharomyces cerevisiae. OSH6, when up-regulated, extends replicative lifespan. In contrast, OSH5 up-regulation shortens replicative lifespan. In this thesis I obtained evidence to show that the deletion of OSH5 extends replicative lifespan in normal media. Deletion of OSH5 in the presence of caloric restriction blocks the lifespan-extending properties of the CR media. My data demonstrate that the total cellular sterol levels of the longevity mutants are indistinguishable from that of wild type. This suggests …

Transport, Deposition, And Hygroscopic Growth Of Diverse-Regime Aerosols In Human Respiratory Airways Of Varying Ages, Jongwon Kim

Theses and Dissertations

Determining how many inhaled aerosols deposit in human respiratory airways and where they deposit (i.e., respiratory dosimetry) are of critical importance in a number of fields. Applications of respiratory dosimetry modeling include evaluating health effects of inhaled pollutants, establishing environmental exposure standards, and developing effective techniques for delivering pharmaceutical aerosols to the lung. However, the manifold characteristics of aerosols coupled with the complexity of respiratory tract make such analysis a challenging task as well as a highly multidisciplinary effort, entailing expertise from medicine, engineering, and physiology. In this study, three major factors that affect particle transport and depositions were systematically …

Novel And Predominant Pathogen Responsible For The Enterovirus-Associated Encephalitis In Eastern China, Lei Zhang, Jie Yan, David M. Ojcius, Huakun Lv, Ziping Miao, Yin Chen, Yanjun Zhang, Jvying Yan

All Dugoni School of Dentistry Faculty Articles

Enteroviruses (EV) have been increasingly identified as the causative agent for unknown etiological encephalitis in many parts of the world, but the long period surveillance for enterovirus-associated encephalitis (EAE) was not reported in China. From 2002-2012 in Zhejiang, Coxsackieviruses A9, B1, B2, B3, B4, B5; and echoviruses 3, 4, 6, 9, 14, 25, 30 were detected from the unknown etiological encephalitis cases, with coxsackievirus B4 been identified here for the first time. From 2002-2004 and 2010-2012, echovirus 30 was found to be the periodically predominant serotype for in the EAE. The molecular typing results showed that all the EV isolates …

A Multifaceted Analysis Of Triple Negative Breast Cancer With High-Throughput Technologies, Aleksandra A. Markovets

Theses and Dissertations

As a subtype of breast cancer, Triple Negative Breast Cancer (TNBC) is characterized by a unique molecular profile, distinct patterns of metastasis and a very aggressive phenotype. Without viable, confirmed therapeutic targets, treatment of TNBC remains one of the most pressing clinical challenges. Hypothesis-driven research on TNBC has focused on a handful of molecules suspected of playing a role in the disease etiology and has yielded limited success. The mechanisms underlying the origin and development of this disease still remain unknown. This gap in understanding the molecular mechanisms in TNBC was critical for undertaking the following analysis of this disease. …

Smokejumper Obituary: Fusko, George M. (Missoula 1962), National Smokejumper Association

Smokejumper Obituaries

No abstract provided.

Spatial Analysis Of Factors Influencing Long-Term Stress In The Grizzly Bear (Ursus Arctos) Population Of Alberta, Canada, Mathieu Bourbonnais, Trisalyn Nelson, Mark Cattet, Chris T. Darimont, Gordon Stenhouse

Physiology Collection

Non-invasive measures for assessing long-term stress in free ranging mammals are an increasingly important approach for understanding physiological responses to landscape conditions. Using a spatially and temporally expansive dataset of hair cortisol concentrations (HCC) generated from a threatened grizzly bear (Ursus arctos) population in Alberta, Canada, we quantified how variables representing habitat conditions and anthropogenic disturbance impact long-term stress in grizzly bears. We characterized spatial variability in male and female HCC point data using kernel density estimation and quantified variable influence on spatial patterns of male and female HCC stress surfaces using random forests. Separate models were developed for regions …

Effects Of Understory Vegetation And Litter On Plant Nitrogen (N), Phosphorus (P), N∶P Ratio And Their Relationships With Growth Rate Of Indigenous Seedlings In Subtropical Plantations, Jun Wang, Dafeng Hui, Hai Ren, Zhanfeng Liu, Long Yang

Biology Faculty Research

Establishing seedlings in subtropical plantations is very important for forest health, succession and management. Information on seedling nutrient concentrations is essential for both the selection of suitable indigenous tree species to accelerate succession of the established plantation and sustainable forest management. In this study, we investigated the concentrations of nitrogen ([N]), phosphorus ([P]), and N∶P ratio in leaves, stems and roots of seedlings of three indigenous tree species (Castanopsis chinensis, Michelia chapensis and Psychotria rubra) transplanted with removing or retaining understory vegetation and litter at two typical subtropical forest plantations (Eucalyptus plantation and native species plantation). We also measured the …

The Jikitou Biomedical Question Answering System: Facilitating The Next Stage In The Evolution Of Information Retrieval, Michael Anton Bauer

Theses and Dissertations

In clinical and biomedical settings researchers often use specialized search engines to acquire answers to technical questions or to verify experimental results through the use of peer reviewed scientific literature. The outcome of such queries typically results in the reading and scanning of multiple Web pages and documents. Information retrieval is the science of retrieving relevant items, question answering (QA) is a specialized type of information retrieval with the aim of returning precise short answers to queries posed as natural language questions. In this dissertation I describe and discuss a QA system named Jikitou (www.jikitou.com), which creates a dialog with …

Inflammatory Cytokine Gene Expression In Mesenteric Adipose Tissue During Acute Experimental Colitis, William Conan Mustain, Marlene E. Starr, Joseph Daniel Valentino, Donald A. Cohen, Daiki Okamura, Chi Wang, B. Mark Evers, Hiroshi Saito

Markey Cancer Center Faculty Publications

BACKGROUND: Production of inflammatory cytokines by mesenteric adipose tissue (MAT) has been implicated in the pathogenesis of inflammatory bowel disease (IBD). Animal models of colitis have demonstrated inflammatory changes within MAT, but it is unclear if these changes occur in isolation or as part of a systemic adipose tissue response. It is also unknown what cell types are responsible for cytokine production within MAT. The present study was designed to determine whether cytokine production by MAT during experimental colitis is depot-specific, and also to identify the source of cytokine production within MAT.

METHODS: Experimental colitis was induced in 6-month-old C57BL/6 …

U.S. Drought Monitor, December 24, 2013, Matthew Rosencrans

United States Agricultural Commodities in Drought Archive

Drought map of U.S. for December 24, 2013 (12/24/13) plus: U.S. crop areas experiencing drought (map), Approximate percentage of crop located in drought, by state (bar graph), Percent of crop area located in drought, past 52 weeks (line graph) for: Hay, Cattle, Winter wheat.

Table Of Contents, Michele Harmon

Journal of the South Carolina Academy of Science

No abstract provided.

Effect Of Nanoparticles On The Biochemical And Behavioral Aging Phenotype Of The Nematode Caenorhabditis Elegans, Andrea Scharf, Annette Piechulek, Anna Von Mikecz

Biological Sciences Faculty Research & Creative Works

Invertebrate animal models such as the nematode Caenorhabditis elegans (C. elegans) are increasingly used in nanotechnological applications. Research in this area covers a wide range from remote control of worm behavior by nanoparticles (NPs) to evaluation of organismal nanomaterial safety. Despite of the broad spectrum of investigated NP-bio interactions, little is known about the role of nanomaterials with respect to aging processes in C. elegans. We trace NPs in single cells of adult C. elegans and correlate particle distribution with the worm's metabolism and organ function. By confocal microscopy analysis of fluorescently labeled NPs in living worms, we identify two …

Shellfish Tissue Monitoring In Piscataqua Region Estuaries 2013, Matthew A. Wood, Philip R. Trowbridge

PREP Reports & Publications

Originally conducted by the Gulf of Maine Council on the Marine Environment from 1993 to 2011, the Gulfwatch Program examined trends in the water quality of the Gulf of Maine by monitoring toxic contaminant concentrations in the tissues of shellfish. Starting in 2012 the Piscataqua Region Estuaries Partnership (PREP) continued this program in the Piscataqua Region. Each year, PREP collects blue mussels at three sites: Dover Point, NH (NHDP), Clark Cove on Seavey Island, ME (MECC), and Hampton-Seabrook Harbor (NHHS). The mussel tissue is analyzed to determine the concentrations of toxic contaminantss including heavy metals, chlorinated pesticides, polychlorinated biphenyls (PCBs), …

Wetlands Utilization And Rural Livelihood Diversification: A Case Of Simiyu Wetland, Magu, Tanzania, Emiliana Mwita

Journal of Humanities and Social Sciences

In many developing countries wetlands play an important role in supporting the livelihood of both urban and rural communities. In rural areas wetlands are increasingly becoming a focal point of production and livelihood diversification due to their richness and the diverse options they provide. This paper explores the livelihood options provided by Simiyu wetland in Magu District, Simiyu Region, to the community living arround it using social survey methods. This paper is based on a study which shows that wetland farming is the main activity (75%) and significantly contribute to cash income generation, followed by livestock keeping, fishing and handicraft. …

Floristic Composition, Diversity And Structure Of The Kimboza Forest In Morogoro Region, Tanzania, David S. Kacholi

Journal of Humanities and Social Sciences

Kimboza forest reserve in Morogoro region, is an important biodiversity hotspot among the Eastern Arc forests of Tanzania. High population pressure and encroachment threatens its conservation values. This is based on a study which aimed at investigating the composition, diversity, and structure of the tree community in the forest. All trees with DBH ≥ 10cm were inventoried from 0.72ha in a total of 18 plots of 20m × 20m placed randomly from the forest edge to the interior. Most important families and species were determined using familial importance value (FIV) and species importance value (IVI) indices, respectively. Diversity was analyzed …

Comparative Genomics And Functional Analysis Of Rhamnose Catabolic Pathways And Regulons In Bacteria, Irina A. Rodionova, Xiaoqing Li, Vera Thiel, Sergey Stolyar, Krista Stanton, James K. Fredrickson, Donald A. Bryant, Andrei L. Osterman, Aaron A. Best, Dmitry A. Rodionov

Faculty Publications

L-rhamnose (Rha) is a deoxy-hexose sugar commonly found in nature. L-Rha catabolic pathways were previously characterized in various bacteria including Escherichia coli. Nevertheless, homology searches failed to recognize all the genes for the complete L Rha utilization pathways in diverse microbial species involved in biomass decomposition. Moreover, the regulatory mechanisms of L-Rha catabolism have remained unclear in most species. A comparative genomics approach was used to reconstruct the L-Rha catabolic pathways and transcriptional regulons in the phyla Actinobacteria, Bacteroidetes, Chloroflexi, Firmicutes, Proteobacteria, and Thermotogae. The reconstructed pathways include multiple novel enzymes and transporters involved in the utilization of L-Rha and …

Understanding Crx-Associated Retinopathies Using Animal Models, Nicholas Minh Abell Tran

All Theses and Dissertations (ETDs)

Cone-rod homeobox: CRX) protein is a "paired-like" homeodomain transcription factor that is essential for regulating rod and cone photoreceptor transcription. Mutations in human CRX are associated with the dominant retinopathies Retinitis Pigmentosa: RP), Cone-Rod Dystrophy: CoRD) and Leber Congenital Amaurosis: LCA), with variable severity. The goal of my dissertation project was to develop and characterize animal models to understand genetic mechanisms of phenotypic diversity in CRX-associated disease. Heterozygous Crx Knock-Out: KO) mice: "+/-") have normal vision as adults and fail to model the dominant human disease.

We generated two Crx Knock-IN: K-IN) mouse models: CrxE168d2: "E168d2") and CrxR90W: "R90W"), which …

The Function Of Carmil1 In Migrating Cells, Marc Edwards

All Theses and Dissertations (ETDs)

This dissertation describes the physiological role of the Capping Protein- CARMIL interaction in migrating cells. I establish the CARMIL-CP complex as a key regulator of lamellipodial actin assembly and of lamellipodial dynamics. Membrane ruffling at the leading edge of motile cells and macropinocytosis were also found to be dependent on the CARMIL1-CP interaction. This is consistent with macropinocytosis and ruffling being dependent on a functional and dynamic lamellipodium.: Kerr and Teasdale, 2009).

In chapter two I demonstrate that the CBR of CARMIL1 is competent to inhibit CP in cells. I show that overexpression of the CBR in cells leads to …

Beef Cattle Body Temperature During Climatic Stress: A Genome-Wide Association Study, Jeremy T. Howard, Stephen D. Kachman, Warren M. Snelling, E. John Pollak, Daniel C. Ciobanu, Larry A. Kuehn, Matthew L. Spangler

Department of Animal Science: Faculty Publications

Cattle are reared in diverse environments and collecting phenotypic body temperature (BT) measurements to characterize BT variation across diverse environments is difficult and expensive. To better understand the genetic basis of BT regulation, a genome-wide association study was conducted utilizing crossbred steers and heifers totaling 239 animals of unknown pedigree and breed fraction. During predicted extreme heat and cold stress events, hourly tympanic and vaginal BT devices were placed in steers and heifers, respect i v e l y. I n d i v i dua l s we r e genotyped with t h e BovineSNP50K_v2 assay and data …

Sensitization Of Human Cancer Cells To Gemcitabine By The Chk1 Inhibitor Mk-8776: Cell Cycle Perturbation And Impact Of Administration Schedule In Vitro And In Vivo, Ryan Montano, Ruth Thompson, Injae Chung, Huagang Hou, Nadeem Khan, Alan Eastman

Dartmouth Scholarship

Chk1 inhibitors have emerged as promising anticancer therapeutic agents particularly when combined with antimetabolites such as gemcitabine, cytarabine or hydroxyurea. Here, we address the importance of appropriate drug scheduling when gemcitabine is combined with the Chk1 inhibitor MK-8776, and the mechanisms involved in the schedule dependence.

Secretion Of Interferon Gamma From Human Immune Cells Is Altered By Exposure To Tributyltin And Dibutyltin, Shanieek Lawrence, Jacqueline Reid, Margaret Whalen

Chemistry Faculty Research

Tributyltin (TBT) and dibutyltin (DBT) are widespread environmental contaminants found in food, beverages, and human blood samples. Both of these butyltins (BTs) interfere with the ability of human natural killer (NK) cells to lyse target cells and alter secretion of the pro-inflammatory cytokine tumor necrosis factor alpha (TNFα) from human immune cells in vitro. The capacity of BTs to interfere with secretion of other pro-inflammatory cytokines has not been examined. Interferon gamma (IFNγ) is a modulator of adaptive and innate immune responses, playing an important role in overall immune competence. This study shows that both TBT and DBT alter secretion …