Life Sciences Commons^™

Mitochondrial Disorders Should Be Considered As A Differential Diagnosis Of Thiamine Deficiency, Josef Finsterer

HCA Healthcare Journal of Medicine

This letter to the editor addresses limitations to diagnosing thiamine deficiency for multisystem disorders and discusses the potential differential diagnoses that must be considered.

Green Connectivity In Metropolitan Jabodetabek: Climate Change And The Challenge Of Urban Forest, Safira Maharani Putri, Hendricus Andy Simarmata

CSID Journal of Infrastructure Development

Urban areas in Indonesia, particularly the Greater Jakarta metropolitan region (Jabodetabek), are increasingly experiencing the adverse effects of climate change, such as the Urban Heat Island (UHI) phenomenon. Rapid urbanization has contributed significantly to rising greenhouse gas emissions and deteriorating environmental quality. Urban forests are one of the key solutions to mitigate these impacts; however, their spatial distribution in Jabodetabek remains suboptimal and poorly connected. This study uses NDVI as a proxy for urban forest density, where “urban forest” refers to vegetated areas within cities as defined by Presidential Regulation No. 60/2020. The research addresses the following question: How has …

Chia Seed Gel Powder As A Clean-Label Enhancer Of Texture, Physicochemical Quality, Antioxidant Activity, And Prebiotic Function In Probiotic Low-Fat Yogurt, Mahmoud E. A. Hamouda, Ratul Kalita, Abdelfatah K. Ali, Pratibha Chaudhary, Pramith U. Don, Omar A. A. Abdelsater, Anjali Verma, Yaser Elderwy

Nutrition, Dietetics, and Food Science Student Research

This study evaluated the effect of incorporating chia seed gel powder (CSGP) as a natural, clean-label stabilizer on the physicochemical, functional, microbiological, microstructural, antioxidant, and sensory properties of probiotic low-fat yogurt (PLFY) during 21 days of refrigerated storage. Six formulations were prepared using 0–2.5% CSGP, including Control (0% CSGP), YOG1 (0.5% CSGP), YOG2 (1.0% CSGP), YOG3 (1.5% CSGP), YOG4 (2.0% CSGP), and YOG5 (2.5% CSGP). Results showed that increasing CSGP levels noticeably enhanced the total solids, protein content, viscosity, hardness, and water-holding capacity of the PLFY (p < 0.05), while consistently reducing syneresis. Antioxidant activity also rose with higher CSGP concentrations, with YOG5 exhibiting the greatest DPPH scavenging activity (35.12%). Confocal laser scanning microscopy revealed a denser and more uniform protein network in PLFY fortified with CSGP, consistent with rheological measurements showing increased storage (G′) and loss (G″) moduli. Probiotic viability significantly increased (p < 0.05) in CSGP-added samples, indicating a potential prebiotic effect of CSGP. Sensory results demonstrated that although higher CSGP levels slightly darkened the yogurt color, body, texture, flavor, and total sensory scores improved markedly, with YOG5 gaining the highest total score (81.77). The results demonstrate that CSGP acts as a highly effective, multifunctional ingredient that enhances texture, stability, probiotic viability, and antioxidant capacity, making it a strong clean-label candidate for developing high-quality, functional probiotic low-fat yogurt.

Semi-Supervised Learning For Annotation And Representation Of Single-Cell Rna Sequencing And Spatial Transcriptomics Data, Haoran Liu

Dissertations

Semi-supervised learning has emerged as a powerful paradigm for analyzing single-cell RNA sequencing (scRNA-seq) and spatial transcriptomics (ST) data, where full annotation is often costly or impractical. scRNA-seq technologies measure the expression of thousands of genes across tens of thousands of cells, whereas ST additionally captures the spatial coordinates of gene expression within intact tissue sections. Annotation is a key step in both scRNA-seq and ST analysis pipelines, aiming to identify cell types, spatial domains, and latent biological structures. However, most existing annotation approaches rely on separate clustering methods that are typically fully unsupervised and fail to leverage side information …

Entropic Forces Near Fluctuating Surfaces, Rubayet Hassan

Dissertations

Nanoscale structures are inherently dynamic due to persistent thermal fluctuations. Even in solid materials, these random deformations, driven by ambient thermal energy, can become significant when their characteristic scale approaches that of the structure itself and strongly influence their overall mechanical behavior. Examples of such structures include crystalline membranes, appearing in diverse forms such as nanotubes, nanoribbons, and kirigami/origami structures. Similarly, biological nanostructures, including lipid membranes, microtubules, actin filaments, and DNA, exhibit extreme flexibility and responsiveness due to their low bending rigidity. Numerous physiological processes are intrinsically linked to these thermal fluctuations, including exocytosis and endocytosis, membrane fusion, pore formation, …

Computational Methods For Single-Cell And Multi-Omic Data Integration And Regulatory Network Inference, Jianlan Ren

Dissertations

Single-cell and multi-omic technologies have transformed the dissection of cellular heterogeneity and regulatory dynamics in health and disease. However, the high dimensionality, technical variability, and biological complexity of these datasets present significant challenges for integration, annotation, and interpretation. In this dissertation, a suite of computational approaches is introduced to address key problems in single-cell and multi-omic data analysis through model-based innovations and applied statistical frameworks.

First, a constrained deep learning framework for single-cell data integration, label transfer, and clustering is proposed. By incorporating biologically motivated constraints into the training process, robust performance is achieved across simulated and benchmark datasets spanning …

Optimal Sizing Of Pv Water Pumping System For Off-Grid Rural Communities, Basma Abulkheir, Eid Gouda, A. A. Hegazi, Amir Abdel Menaem

Mansoura Engineering Journal

A large population worldwide lives in remote rural communities without access to electricity due to the limited reach of national transmission networks. This creates significant challenges in accessing water, hindering improvements in living conditions for off-grid communities. Photovoltaic water pumping systems (PVWPSs) offer a viable solution to this issue, but their sustainability must be examined due to the unpredictable nature of solar radiation. This paper presents a practical techno-economic approach to optimally size a PVWPS. A Particle Swarm Optimization (PSO) algorithm is employed, considering technical and economic indicators like life cycle cost, loss of load probability and PV curtailment rate. …

Environmental Science And Sustainable Development: Behavioral Transformation To Knowledge System Integration For Sustainability Action, Ahyahudin Sodri, Herdis Herdiansyah

Journal of Environmental Science and Sustainable Development

Sustainability issues are now a global concern. However, knowing the root causes of problems and the target is not the only factor that constantly motivates change for the better (Wamsle & Osberg, 2022) as such change also requires encouragement from actors, including individuals, organizations, and communities. Therefore, the human aspect is crucial for the successful achievement of sustainability (Hariram et al., 2023). In other words, questions on sustainability are not just about "what should be done?" and also on “how to get people to do it?” and “why are currently available interventions not yet fully utilized?” …

Change In Community Composition And Structure On Subtidal Rock Walls In The Gulf Of Maine Over Four Decades Of Warming, Breck A. Mccollum

Graduate Doctoral Dissertations

The Gulf of Maine (GOM) has faced many changes over the last 47 years from overfishing of its iconic cod fish stocks to the introduction on non-native species, but no change has been as dramatic as the warming ocean temperatures that began to increase rapidly in the 2010s. This thermal shift is due largely to the northward movement of the Gulf Stream current mixing with Arctic meltwater before entering the GOM. Average summer temperatures jumped by 3.35°C between the periods of 1980-1985 and 2015-2020. This warming trend, combined with an increasing frequency of marine heat wave events, has led to …

Intercontinental Variation In Population Genetics And Life-History Traits In Native And Invasive Populations Of The Aquatic Plants Myriophyllum Spicatum And Myriophyllum Sibiricum, Anastasia V. Mozharova

Graduate Doctoral Dissertations

Biological invasions pose significant threats to freshwater ecosystems worldwide, and understanding their evolutionary, genetic, and biogeographic drivers is essential for effective management. My dissertation integrates microsatellite and ITS rDNA data to examine the dynamics of both invasive and native populations of two closely related aquatic plant species, Myriophyllum spicatum (Eurasian watermilfoil) and M. sibiricum (Northern watermilfoil).

Myriophyllum spicatum is native to Eurasia, where it inhabits a variety of freshwater aquatic habitats. Since its introduction to North America in the mid-20th century, it has become highly invasive, forming dense underwater mats that outcompete native vegetation, reduce biodiversity, and degrade water quality. …

Investigating The Role Of Cornichon-Homolog 3 (Cnih3) In Opioid Use, Contributing Risk Factors, And Associated Neural Substrates, Tania Lintz

Arts & Sciences Graduate Student Theses and Dissertations

Opioid misuse remains rampant as potent synthetic opioids such as fentanyl flood the market. Large-scale genetic tools like the GWAS identify previously unrecognized targets and biomarkers in opioid misuse with the hopes of combating the opioid epidemic and opioid use disorder (OUD). One such target is the AMPA receptor (AMPAR) auxiliary protein Cornichon Homolog-3 (CNIH3), which determines AMPAR subunit composition and kinetics. Though CNIH3 was identified as a gene of interest in OUD, its role in opioid use and accompanying risk factors has not been studied. Using mice with a CNIH3 deletion, we assess the role of CNIH3 in risk …

Biomarker And Age At Onset Endophenotypes Offer New Insights Into The Genetic Etiology Of Alzheimer’S Disease, Joseph Bradley

Arts & Sciences Graduate Student Theses and Dissertations

Introduction: Alzheimer’s Disease (AD) remains among the top 10 causes of death in the United States and is projected to affect nearly 14 million people by 2050. To this point, studies of Alzheimer’s genetics and endophenotypes have yielded a bounty of results that culminated in the first disease-modifying drugs, lecanemab and donanemab, in 2022. Most of the genetic studies for Alzheimer’s disease to date have been focused on late-onset AD or Autosomal dominant AD, leaving a critical need for studying sporadic early-onset AD (EOAD; ~10% of cases). Furthermore, the demand for clinical portability has created a need for high-sensitivity proteomic …

Construction And Data-Driven Analysis Of A Stochastic, Individual-Based Opioid Epidemiology Network Model, Leigh Bennett Pearcy, Owen Queen, Vincent Jodoin, Suzanne Lenhart, Christopher Strickland

Mathematical Modelling and Numerical Simulation with Applications

While substance use epidemiology has been an active area of mathematical research in recent years, the social and mental processes that are involved in the development of substance use disorders have presented challenges to advancing the epidemiological theory and how they differ from the contraction of pathogenic disease. Such distinction is especially pertinent in the context of the current United States opioid epidemic and its intersection with the recent COVID-19 pandemic, as both prescription drugs and social influence play major roles in the development of opioid use disorder. In this paper, we construct a stochastic network model capturing how individual …

Efficient Task Allocation Technique For Robots In Smart Warehouse Using Center Of Items (Coi) Methodology, ‪Ahmed Yahya‬‏, Mohamed S. Saraya, Ahmed I. Saleh

Mansoura Engineering Journal

Nowadays, online shopping is very important in Daily life. in 2020 During the quarantine during the spread of COVID-19, online orders increased dramatically, as a result of the great progress in fifth-generation technologies and using the Internet of Things (IoT) and industrial Internet of Things (IIoT), industry 4.0 and 5.0, fog computing, cloud computing, robots systems, and drones systems. Smart warehouses picking system becomes a Basic need and can be implemented to improve efficiency in logistics. Smart warehouses are growing more dependent on robot systems to make the process of meeting these requests more flexible and effective. Therefore, the allocation …

Gas-Flux-Determination-Method Effects On Greenhouse Gas Emissions Andglobal Warming Potential From Soybean In Southeast Arkans, Jonathan B. Brye, Diego Della Lunga, Kristofor R. Brye

Crop, Soil and Environmental Sciences Faculty Publications and Presentations

Greenhouse gas (GHG) research and quantification have increased in importance recently due to climate change. Non-flow-through chamber systems collect large quantities of gas concentration data to determine the gas flux, but gas-flux-determination (GFD) methods can vary between regression models, how negative fluxes are considered, and what agroecosystem is evaluated, which makes selecting an analysis method complicated. The objective of this study was to evaluate the effects of GFD method [i.e. linear (L) and exponential (E) regression models, with negative fluxes (WNF) and with reassigned negative fluxes (RNF)] on GHG fluxes, emissions, and global warming potential (GWP) from soybean (Glycine max) …

Speeding Up Interval Estimation For R2-Based Mediation Effect Of High-Dimensional Mediators Via Cross-Fitting, Zhichao Xu, Chunlin Li, Sunyi Chi, Tianzhong Yang, Peng Wei

Faculty, Staff and Student Publications

Mediation analysis is a useful tool in investigating how molecular phenotypes such as gene expression mediate the effect of exposure on health outcomes. However, commonly used mean-based total mediation effect measures may suffer from cancellation of component-wise mediation effects in opposite directions in the presence of high-dimensional omics mediators. To overcome this limitation, we recently proposed a variance-based R-squared total mediation effect measure that relies on the computationally intensive nonparametric bootstrap for confidence interval estimation. In the work described herein, we formulated a more efficient two-stage, cross-fitted estimation procedure for the R2 measure. To avoid potential bias, we performed …

Netrin-1 Promotes Pancreatic Tumorigenesis And Innervation Through Neo1, Hiroki Kobayashi, Yosuke Ochiai, Junya Arai, Masahiro Hata, Feijing Wu, Masaki Sunagawa, Tadashi Iida, Taisuke Baba, Ermanno Malagola, Takayuki Tanaka, Zhengyu Jiang, Ruth A White, Xiaofei Zhi, Jin Qian, Quin T Waterbury, Ruhong Tu, Biyun Zheng, Yi Zeng, Hualong Zheng, Puran Zhang, Shuang Li, Leah B Zamechek, Jonathan S Labella, Takahiro Sugie, Atsushi Enomoto, Holger K Eltzschig, Carmine F Palermo, Iok In Christine Chio, Kenneth P Olive, Timothy C Wang

Faculty, Staff and Student Publications

Nerves can regulate tumorigenesis and cancer progression. However, clarification of the role of axon guidance molecules in tumorigenesis, innervation, and metastasis is required to better understand the tumor-promoting functions of nerves. Using murine KrasG12D-mutant pancreatic organoids, we screened axon guidance molecules and identified netrin-1 upregulation. Netrin-1 was also upregulated in vivo during pancreatic tumorigenesis in humans and mice. Mutant KRAS and β-adrenergic signaling upregulated netrin-1 and its receptor NEO1 in epithelial cells in part through the MAPK pathway. Ex vivo culture of celiac ganglia showed that netrin-1 promoted the axonogenesis of sympathetic neurons through nerve NEO1. In the Pdx1-Cre;LSL-KrasG12D/+ model, …

Investigating The Incorporation Of Milled Rice In Brewing Non-Alcoholic Beer To Enhance Sensory Quality, Christian Schubert, Rahul Sen, Nils Rettberg, Scott Lafontaine

Food Science Faculty Publications and Presentations

The influence of substituting barley malt with milled rice on the sensory and chemical characteristics of nonalcoholic beers (NAB) was investigated. Physicochemical and volatile analyses were conducted on the NABs. Original gravities ranged from 6.71% w/w (100% barley malt) to 7.15% w/w (100% rice), with alcohol contents between 0.33% v/v (70% barley/30 %rice) and 0.45 % v/v (100% rice). Color decreased from 10.8 EBC (100% barley malt) to 3.3 EBC (100% rice). Descriptive analysis sensory evaluations by trained panelists in the United States and European Union were used to characterize the sensory profiles of the NABs. Principal component analysis showed …

Baseline Assessment Of Insect Biodiversity And Distribution In La Salle Botanical Gardens, Pampanga, Philippines, Amir Lyndon Gavriell C. Beup, Kaleela Euan G. Jayson, Alyssa Ira Z. Tan, Ronielle Elisha S. Pascual, Alexi Gabrielle S. Henson, Esperidion Josef P. Ladao Iii, Juan Carlo T. Gumangan

Sinaya: A Philippine Journal for Senior High School Teachers and Students

Insects are crucial for ecosystem functions, such as pollination and food web maintenance. This study aims to assess insect biodiversity in La Salle Botanical Gardens (LSBG) in Porac, Pampanga, focusing on its grassland and forestland areas. Using rapid biodiversity assessment, this study provides baseline data on insect diversity, abundance, dominance, and evenness for conservation. Sampling employed transect line intercept and quadrat methods. Specimens were collected using different collection and preservation techniques. Insects (n = 184) were collected from August to October 2024, with specimens (n = 46) belonging to seven insect orders in the grassland and specimens (n = 138) …

Comparing Individual Vs Mixed Treatments Of Chicken Bone Meal And Crab Shell Meal On The Growth Of Capsicum Annuum (Bell Pepper), Allthur Rudy R. Arroyo, John Rafael V. Capinia, Ethan Jude R. Reyes, Elnathan Jethro B. Sumalbag, Karl Benedict E. Viri, John Ray Cordova Estrellado, Kerry P. Cabral

Sinaya: A Philippine Journal for Senior High School Teachers and Students

The recycling of nutrient-rich food waste reduces reliance on synthetic fertilizers, enhances soil quality, and improves plant growth. Existing studies have demonstrated the effectiveness of food waste, such as bone meal (B) and crab shell meal (S), as fertilizers; however, their combined use has not been investigated. This study aims to evaluate the effect of varying proportions of B and S fertilizers on the growth of Capsicum annuum (bell pepper) employing a random complete block design with 10 treatments (pure B, pure S, and a 50:50 BS mix, each at 7 g, 14 g, and 21 g) plus a control …

Cesium-137 Accumulation In Marine Fish And Associated Dose Exposure: A Global Review, N.A.A. Kamarulzaman, M.S.M. Sanusi, H.N. Idris

Journal of King Abdulaziz University: Marine Science

Establishing baseline data for natural radiation exposure and radioactivity levels is essential for radiological health and safety programs, particularly during the implementation and development of nuclear power initiatives. This review evaluates gamma radiation exposure and anthropogenic cesium-137 (¹³⁷Cs) levels in the world’s oceans and marine fish, analyzing their radioecological impact. Data from 30 peer-reviewed publications indicate that ¹³⁷Cs activity concentrations in seawater range from 0.07 Bq kg^–1 to 6.4 kBq kg^–1, with fish values between 0.01–3.50 kBq kg^–1. Regression models developed from these studies predict the correlation between ¹³⁷Cs levels in …

Lead Bioremoval By Halotolerant Bacteria Isolated From Mangrove Rhizosphere Soils Of Saudi Arabia, Reda H. Amasha, Rawan A. Alhazzani

Journal of King Abdulaziz University: Marine Science

Bioremediation of hypersaline environments contaminated with heavy metals is challenging due to high salinity levels, particularly when using conventional microorganisms. This study aimed to isolate and identify halotolerant and halophilic bacteria from various mangrove rhizosphere soil samples in Saudi Arabia and to evaluate their ability to remove heavy metals under high-salinity conditions. Out of 27 isolated strains, LD57 and LR40 demonstrated the highest resistance to lead ions. Both isolates grew well at 37 °C in the presence of 3 M NaCl and recorded growth at varying concentrations of lead nitrate, ranging from 100 to 4500 ppm. The lead’s minimum inhibitory …

Molecular Docking And Antibacterial Activity Of Sesterterpenoids From Hyrtios Erectus, Hajer S. Alorfi, Nawal A. Albalawi

Journal of King Abdulaziz University: Marine Science

The search for new antibiotics remains urgent due to the continuous evolution of microbes and their resistance to currently available antibiotics. The organic extract of a specimen of the Red Sea sponge Hyrtios erectus collected off Jeddah coast was subjected to sequential chromatographic fractionation. Three sesterterpenoids (1–3) along with an indole alkaloid (4) were isolated from H. erectus. Structure elucidation of the isolated was accomplished using NMR, UV, and IR spectroscopy along with MS spectrometry. The antibacterial activity of the isolated sesterterpenoids (1–3) was assessed against a set of Gram-positive and negative …

A Systematic Literature Review Of Sustainable Ports Strategies And Initiatives In The Arabian Gulf Ports – Lessons For Saudi Arabia And Uae, Osayd Helal, Jane Haider, Jean-Paul Skeete, Sehwa Lim

Journal of King Abdulaziz University: Marine Science

This systematic review provides a comprehensive evaluation of sustainability strategies in Arabian Gulf ports, focusing on the interplay between technological advancements, green initiatives, and regulatory challenges. While Industry 4.0 technologies—such as automation, artificial intelligence, and electrification—have been integrated to enhance operational efficiency and reduce carbon emissions, the extent to which these innovations align with regional policy frameworks and geopolitical constraints remains insufficiently analysed.

To address this gap, this review synthesises insights from 25 peer-reviewed studies, employing a comparative framework to assess the adoption of sustainability practices in Arabian Gulf ports, focusing on their approaches toward sustainable port development. It is …

Assessment Of Microplastic Pollution In Economically Significant Fish Species From Libya’S Northeastern Mediterranean Coast, Mousa Kateesh, Tarek A. Temraz, Rafat Afifi Khattab

Journal of King Abdulaziz University: Marine Science

Microplastic pollution in marine ecosystems has become a pressing global environmental issue. However, there is limited information on the distribution of microplastics in Libyan territorial waters. This study investigates plastic waste pollution in five commercially important fish species from various marine habitats along the eastern Libyan coast (Ain El-Ghazala, Sousa, Ras Hilal, Alhaniyah, and Alhamama). A total of 75 fish specimens were collected, comprising 15 individuals from each species. The findings revealed that 38% of the sampled fish had ingested plastic debris, with microplastics being the predominant form. The Sousa region exhibited the highest contamination rate (54.46%), while Alhaniyah had …

Urbanization Impacts On Microplastic In Benthic Organisms And Sediment Of Small Islands, Andrio Wibowo, Suyud Warno Utomo

Journal of King Abdulaziz University: Marine Science

Indonesia is an archipelago country comprising thousands of islands, including small islands. Currently, small islands have experienced urbanization in the forms of land use developments and conversions from pristine ecosystems to settlements and tourism facilities, leading to the contamination of microplastics. Despite growing numbers of research on microplastic in water, there is still limited information about microplastic in the sediment and benthic, in particular in developed small island environments. This study compared the types of land use on the island from reserved areas, tourism, and settlement. The microplastics were characterized based on the shapes. The results confirm that land use …

New Fish-Based Multimetric Index For Appraising Shatt Al-Arab Ecological Quality, Laith Faisal Lazem

Journal of King Abdulaziz University: Marine Science

In recent years, there has been a growing need to develop biometric indicators that accurately reflect an area’s environmental quality and biological integrity. Various indicators have been developed and analyzed, focusing on functional, ecological, and organism-based indicators, particularly fish-related ones. The purpose of the current paper is to create the first fish-based multimetric index designed to evaluate the quality of water in the Shatt Al-Arab River (SAAR), known as Shatt Al-Arab Fish Index (SFI), which can also be applied to the Qarmat Ali River and other water bodies similar to the SAAR ecology. Data was collected from three stations along …

Trajectory Prediction Of Oil Spills In Sharm Obhur, Red Sea Using Hydrodynamic Modeling, Mohammed A. Bahattab, Gar Al-Nabi Ibrahim, V.R. Shamji

Journal of King Abdulaziz University: Marine Science

This research investigates the movement and impact of oil spills in Sharm Obhur, a coastal creek near Jeddah, in the Red Sea. Utilizing a high-resolution multi-beam echo sounder and the Delft3D-FLOW hydrodynamic simulation model, the study traces the oil spill trajectories, considering key factors such as meteorological conditions, spill volume, and oil properties. The model predicts the movement of the oil spill both within Sharm Obhur and its progression towards the Red Sea. Results from the model are intended to assist in the development of effective spill response strategies, improving navigation safety, protecting coral reefs, and preserving the local ecosystem. …

Preprocessing Improves The Edna Quality For 16s Rrna Metabarcoding Of Sediment Samples Of Solar Salterns, Jayanath Gopi, Bhavya Kachiprath, Solly Solomon, Sowmya P. Mohandas, Sajan C. Raju, Rosamma Philip

Journal of King Abdulaziz University: Marine Science

The present study examines the efficacy of pre- processing of sediment samples prior to DNA isolation to obtain high-quality eDNA for metagenomic analyses from crystallizer saltern pond in Goa during the salt harvesting phase. The quality of the extracted eDNA were confirmed through spectrophotometric analysis and PCR amplification of the 16S rRNA gene. The study showed that method 2 and 3 incorporating preprocessing of the sediment samples yielded better quality eDNA compared to the direct isolation protocol (method1). By adopting the best method i.e., preprocessing by sediment suspension- filtration, followed by DNA isolation, the eDNA was procured and subjected to …

Morphometric, Physiological, And Environmental Health Assessments Of Enhalus Acoroides From The Southeast Coast Of Bali, Indonesia, Made Pharmawati, Luh Putu Wrasiati, Ni Made Gari

Journal of King Abdulaziz University: Marine Science

Seagrasses function as primary producers and as spawning grounds and shelter for marine biota. Regardless of their relevance, seagrasses are under threat due to anthropogenic impacts and an increasing frequency of extreme climate events. Enhalus acoroides is a species of seagrass that is spread in the east and southeast waters of Bali, from Sanur Beach to Nusa Dua Beach. This study aims to analyze the differences in morphometry and the chlorophyll, proline, and malondialdehyde (MDA) content, a marker for oxidative stress in E. acoroides leaves at Pengastian Beach, Semawang Beach, and Samuh Beach, Bali. Samples were collected using line transect …