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Full-Text Articles in Life Sciences

Long-Term Compositional Shifts In The Fish Community Of The Wabash River, Olivia R. Wertman Jan 2026

Long-Term Compositional Shifts In The Fish Community Of The Wabash River, Olivia R. Wertman

Masters Theses

There is concern about declining biodiversity in the world today and an increasing need to monitor biodiversity through collecting data and gauging changes occurring in biodiversity over time. Large river fish communities are one area of particular interest due to their sensitivity to environmental changes. One program seeking to address this is the Long-Term Survey and Assessment of Large River Fishes, LTEF, which monitors fish populations in the large rivers of Illinois. In this study, I assessed changes in the fish community of the lower 322 km of the Wabash River from 2010-2025 using annual direct current boat electrofishing surveys …


If You Build It, Will They Stay? Fish Communities Continue To Utilize Restored Habitat A Decade Following An Instream Restoration, Alexis L. Vandenberg Jan 2026

If You Build It, Will They Stay? Fish Communities Continue To Utilize Restored Habitat A Decade Following An Instream Restoration, Alexis L. Vandenberg

Masters Theses

Lotic ecosystems are degraded at an alarming rate due to anthropogenic activities. In order to combat these changes, stream restorations are implemented to improve conditions such as water quality, habitat heterogeneity, and bank stability. However, the impacts of these restorations are often understudied and long-term impacts are unknown. Restorations are typically designed to be ‘fish attractors’; this idea is known as the ‘Field of Dreams hypothesis’ – ‘if you build it, they will come’. This study uses data collected in Kickapoo Creek, Coles County from 2009 to 2015 and again in 2021 and 2022 to assess the long-term impacts of …


Compositional And Functional Shifts In Fish Community Structure In Response To Low-Head Dam Removals, Joshua D. Bruegge Jan 2026

Compositional And Functional Shifts In Fish Community Structure In Response To Low-Head Dam Removals, Joshua D. Bruegge

Masters Theses

Low-head dams serve as intermittent barriers to aquatic connectivity and directly alter both upstream and downstream habitats and biotic communities. In 2018 and 2019, two low-head dams in the Vermilion River watershed of eastern Illinois were removed, reconnecting over 1,700 kilometers of upstream habitat within the basin. To determine the impacts of these dam removals, fish community surveys were conducted at 12 fixed sites across three reaches in two rivers from 2012-2022. This study assessed reach-specific temporal responses of the fish community and habitat characteristics. I linked trait expressions within the fish community to abiotic variables using the co-inertia RLQ …


Feasibility Of Corn Stover For Biogas Production In Northwestern Illinois: A Case Study Of Jo Daviess And Carroll Counties, Anthony Amotoe-Bondzie Jan 2026

Feasibility Of Corn Stover For Biogas Production In Northwestern Illinois: A Case Study Of Jo Daviess And Carroll Counties, Anthony Amotoe-Bondzie

Masters Theses

The growing demand for renewable energy and sustainable waste management has intensified interest in agricultural residues such as bioenergy feedstocks. This study evaluated the feasibility of converting corn stover into biogas through anaerobic digestion (AD) in Jo Daviess and Carroll Counties, Illinois, with consideration of the Savanna Industrial Park as a potential centralized processing hub. Although corn stover represents one of the largest biomass resources in the United States, this research identifies a critical gap between theoretical availability and practically recoverable feedstock. Using a mixed-methods framework, the study integrates biomass quantification, methane yield modeling, economic analysis, and policy assessment. Results …


Epigenetic Modifications In Dedifferentiated Liver Cells To Restore Lost Liver Function, Shreya Sharma Jan 2026

Epigenetic Modifications In Dedifferentiated Liver Cells To Restore Lost Liver Function, Shreya Sharma

Masters Theses

The goal of the study is to investigate whether the epigenetic modifications can enhance the restoration of liver gene expression in dedifferentiated hepatoma cells or not. For the study, we used dedifferentiated H11 hepatoma cell lines which overexpress one of a panel of introduced candidate liver-enriched transcription factors H11-HNF1a, H11-CREB3L3, H11-CREG1, H11- HHEX, H11-HNF6, and H11-IGFBP1. The cells were treated with the DNA methyltransferase inhibitor 5-azacytidine and the histone deacetylase inhibitor sodium butyrate, both individually and in combination. Gene expression analysis was determined by RT-qPCR, which revealed that while transcription factor overexpression alone produced limited activation, epigenetic modulation, particularly Histone …


Butterflies & You: Considerations Of Human Relationships With The Natural World, M. E. Colby Dec 2025

Butterflies & You: Considerations Of Human Relationships With The Natural World, M. E. Colby

Undergraduate Honors Theses

Science communication only accounts for approximately 2% of the entire scientific output of 2017-2022 (Parejo-Cuéllar et. al, 2024). It is an under-researched and under-valued field, especially in an age that has devalued science as a whole. The delicate art of explaining complicated ideas to people who are unfamiliar with scientific jargon is vital to humans’ understanding of the world. Butterflies & You is a documentary film exploring the relationship between humans and the many different butterfly species that call Illinois home. Video-based science communication is important to research and practice because of its impact on environmental advocacy and the growing …


2025 Celebration Of Scholarship, Creativity, And Engagement, Todd Bruns, Jaime Pedigo, Jennifer Smith, Patty Watson, Heather Wohltman, Amanda Wolf Oct 2025

2025 Celebration Of Scholarship, Creativity, And Engagement, Todd Bruns, Jaime Pedigo, Jennifer Smith, Patty Watson, Heather Wohltman, Amanda Wolf

Programs

Each year, Eastern Illinois University proudly recognizes the outstanding achievements of our faculty in research, creativity, and engagement. This collection of accomplishments reflects the dedication of an engaged faculty committed to excellence and the continual pursuit of knowledge. Many of these successes were made possible through meaningful collaboration with our students, who contribute as co-authors, research assistants, and partners in bringing ideas to life. Some of these professional milestones represent years of effort, demonstrating remarkable persistence and determination. We offer our heartfelt congratulations on this wide range of achievements, which bring distinction to our departments, colleges, and the University as …


How Can The Adoption Of Biodegradable Packaging In The Beverage Industry Specifically Contribute To Reducing Single-Use Plastic Waste, And What Regulatory Or Cost-Related Challenges Hinder Its Widespread Implementation In Developing Countries?, Rebecca Aba Gaisie Apr 2025

How Can The Adoption Of Biodegradable Packaging In The Beverage Industry Specifically Contribute To Reducing Single-Use Plastic Waste, And What Regulatory Or Cost-Related Challenges Hinder Its Widespread Implementation In Developing Countries?, Rebecca Aba Gaisie

2025 Awards for Excellence in Student Research and Creative Activity - Documents

This research investigates the potential of biodegradable packaging to reduce single-use plastic waste in the beverage industry and explores the regulatory and cost-related barriers to its adoption in developing countries. The beverage sector is a major contributor to global plastic pollution, and biodegradable alternatives offer a promising path toward sustainability. Using a mixed-methods approach, this study combines qualitative interviews with industry experts and case studies of businesses implementing biodegradable solutions, alongside quantitative survey data from 100 manufacturers and 200 consumers across Africa, Asia, and Latin America. Findings reveal that while biodegradable packaging significantly reduces plastic waste and appeals to environmentally …


Are There Predictable Shifts In Fish Communities Following Instream Habitat Restoration? A Multistream Analysis., Ryan A. Skowronski Jan 2025

Are There Predictable Shifts In Fish Communities Following Instream Habitat Restoration? A Multistream Analysis., Ryan A. Skowronski

Masters Theses

In the past two centuries, anthropogenic stressors have degraded the structure and function of streams by accelerating bank erosion, decreasing riparian buffer zones, and altering the natural flow regime. Habitat restoration has become a common mitigation strategy in response to stream degradation, though empirical research evaluating its effectiveness is lacking in the United States. This study investigated responses in fish community following habitat restoration in five restored, Illinois streams. We assigned fish species to habitat, trophic, and reproductive guilds based on their habitat preferences, feeding habits, and life history. Streams were separated into enhancement and removal groups for analysis. Shifts …


Parasite Communities Of Bluntnose Minnow (Pimephales Notatus) From Several Creeks In East Central Illinois, Md Rowshan Alam Jan 2025

Parasite Communities Of Bluntnose Minnow (Pimephales Notatus) From Several Creeks In East Central Illinois, Md Rowshan Alam

Masters Theses

Parasites of freshwater fish play a vital functional role in aquatic ecosystems, but the spatial and ecological determinants of their prevalence and characteristics are still poorly understood. The current study quantified the diversity, prevalence, and environmental determinants of whole parasite burdens in bluntnose minnows across multiple creeks in central Illinois. Fish sampling and the collection of stream habitat data were conducted during the summers of 2023 and 2024. Fish were examined for parasites using necropsy and histology. A total of 20,817 parasites, predominantly trematodes (Posthodiplostomum spp.), cestodes (Proteocephalus spp), and protozoans, were identified. The most prevalent and abundant …


Assessing The Role Of Daily Thermal Fluctuations In The Performance Of Mitochondrial Energy Transduction Of The Centrarchid, Lepomis Cyanellus, Grayson Hofmann Jan 2025

Assessing The Role Of Daily Thermal Fluctuations In The Performance Of Mitochondrial Energy Transduction Of The Centrarchid, Lepomis Cyanellus, Grayson Hofmann

Masters Theses

Freshwater systems are affected by current climate extremes, forcing species found in these systems to withstand drastic changes in temperature. Temperature changes affect these organisms by altering their metabolic rate and energy balance. Increases in temperature can lead to an increase in oxygen consumption, which ultimately increases the need for more energy production. Mitochondrial energy transduction of green sunfish (Lepomis cyanellus) and many other fish species after a thermal insult, has hardly been explored. This study complements the limited research available on mitochondrial function in similar species, as they ignore the possibility of consecutive thermal events, which are …


Habitat Use And Selection Of Silver Carp In The Lower Wabash River System, Coy B. Blair Jan 2025

Habitat Use And Selection Of Silver Carp In The Lower Wabash River System, Coy B. Blair

Masters Theses

The spatial ecology of non-native species in invaded systems remains relatively poorly understood despite the importance of suitable habitat in the success of invasion. Silver Carp Hypophthalmichthys molitrix are a highly invasive non-native species with established populations throughout the Midwestern United States. Much research has focused on habitat use of bigheaded carp in large river systems, though invasion risk remains high in smaller systems, such as tributaries of the Great Lakes and the Ohio River. The Wabash River, a unique system in the Midwest, remains largely un-impounded and is comparable to many of the smaller riverine systems at risk of …


Examining Group Components On Exercise Adherence In Older Adults, Brody Polk Jan 2025

Examining Group Components On Exercise Adherence In Older Adults, Brody Polk

Masters Theses

Older adults remain the least active population despite the well-known benefits of regular exercise (Beauchamp et al., 2007; Rúa-Alonso et al., 2023). Researchers examining exercise adherence in older adults have determined two modalities of training—individual and group—with individual training being the preferred mode of exercise for older adults (Beauchamp et al., 2007). However, previous research has failed to evaluate the various components of group exercise before making a comparison with individual exercise to determine which form best predicts older adults’ adherence to exercise (Hawley-Hague et al., 2014). As such, the purpose of the current study was to examine the effects …


Prevalence Of Insecticide Resistance Gene In Aedes Albopictus Populations Of Central Illinois, Merylann Akinyi Ochieng Jan 2025

Prevalence Of Insecticide Resistance Gene In Aedes Albopictus Populations Of Central Illinois, Merylann Akinyi Ochieng

Masters Theses

The Asian tiger mosquito, Aedes albopictus, is a significant vector for diseases such as Dengue, Chikungunya, and Zika. Its increasing resistance to insecticides poses a critical challenge for vector control efforts. This study investigates knockdown resistance (kdr) mutations in the voltage-gated sodium channel (VGSC) genes, specifically V1016G and F1534C, which are associated with pyrethroid resistance. Aedes albopictus populations from Central Illinois were analyzed using allele-specific PCR techniques to address three primary objectives: (1) determining whether V1016G and F1534C mutations are present in these populations, (2) determining which mutation is more widespread, and (3) investigating how resistance gene …


Enhancing Sunflower Regeneration: Molecular And Genetic Insights From Helianthus Eggertii, Owen West Jan 2025

Enhancing Sunflower Regeneration: Molecular And Genetic Insights From Helianthus Eggertii, Owen West

Masters Theses

In vitro plant regeneration, particularly through organogenesis and somatic embryogenesis, is essential for plant transformation and crop improvement, especially in recalcitrant species like sunflower. In this study, we explore the genetic and molecular mechanisms underlying regeneration in Helianthus eggertii, a wild sunflower species with high regeneration potential. Global gene expression profiling, conducted using Oxford Nanopore sequencing across multiple time points and genotypes, identified sixty-three core differentially expressed genes (DEGs) critical to regeneration. These DEGs are associated with critical biological processes such as cell proliferation, transcriptional regulation, and photosynthesis. Among them, a sunflower WUSCHEL-related homeobox transcription factor (WOX4) showed preferential expression …


Spatiotemporal Variability In Invasive Carp Spawning Activity In Two Midwestern River Basins, Braden A. Whisler Jan 2025

Spatiotemporal Variability In Invasive Carp Spawning Activity In Two Midwestern River Basins, Braden A. Whisler

Masters Theses

Across the United States, fisheries managers and agencies continue to battle the spread of invasive bigheaded carp (Hypophthalmichthys spp.). Although previous research has identified environmental parameters such as temperature and flow to be triggers of spawning activity, many of these studies focus on larger rivers, rarely focusing on the specific thresholds or timing of when a spawning event takes place. I utilized the differing hydrology of the Illinois and Wabash Rivers and their tributaries to examine the differences in environmental parameters needed to trigger bigheaded carp reproduction. Three mainstem sites and three tributaries of both basins were sampled …


Assessing Ecological And Food Web Responses To Small-Scale Stream Restorations In Urban And Agricultural Streams, Sydney Mcandrews Jan 2025

Assessing Ecological And Food Web Responses To Small-Scale Stream Restorations In Urban And Agricultural Streams, Sydney Mcandrews

Masters Theses

Urban and agricultural streams across the Midwestern United States are vulnerable to elevated pollution, eutrophication, habitat simplification, and losses of sensitive species. Instream habitat restoration is increasingly used to mitigate the impacts of rapid land-use change. This study synthesizes habitat and biological responses from a 2020 urban stream restoration in the Saline Branch (Urbana, Illinois) and evaluates food web responses from both the Saline Branch and a 2010 restoration in Kickapoo Creek (Charleston, Illinois), an agricultural stream. In the Saline Branch, four years of fish and three years of habitat and macroinvertebrate pre-restoration surveys were compared to three years of …


Evaluation Of Blue Catfish Populations In Four Midwestern Power Cooling Reservoirs, Tyler Joseph Murray Jan 2025

Evaluation Of Blue Catfish Populations In Four Midwestern Power Cooling Reservoirs, Tyler Joseph Murray

Masters Theses

Blue Catfish (Ictalurus furcatus) are the largest catfish in North America and as such angler desire for trophy fisheries have grown in addition to harvest fisheries. The Illinois Department of Natural Resources (IDNR) has stocked Blue Catfish into several reservoirs in the state including four that have powerplants that utilize the water for cooling and discharge it back into the reservoir at increased temperatures. This study explored the potential impacts of these warm water effluents on Blue Catfish population demographics, as well as whether management changes need to be implemented in these systems. I sampled Powerton, LaSalle, Springfield, …


Soils From Different Solidago Altissima Clones Mediate Intraspecific Interactions In Schizachyrium Scoparium, Sarah Alharbi Jan 2025

Soils From Different Solidago Altissima Clones Mediate Intraspecific Interactions In Schizachyrium Scoparium, Sarah Alharbi

Masters Theses

While previous studies have examined plant intraspecific competition and soil microbial effects separately, fewer studies have investigated how these factors interact, particularly in the context of genotype-specific soil conditioning effects. Plant-soil feedbacks can significantly alter interspecific competitive outcomes, yet few studies have examined how local variation in soil conditioning influences plant responses to density-dependent competition. I investigated how soil inocula derived from four Solidago altissima clones that were previously shown to differ in their soil conditioning effects (Foster et al., 2022) affected the growth and root traits of Schizachyrium scoparium across a gradient of intraspecific competition (1, 2, or 4 …


Barriers To Leisure-Time Physical Activity Among College Students, Ben Andrew Winfield Jan 2025

Barriers To Leisure-Time Physical Activity Among College Students, Ben Andrew Winfield

Masters Theses

Obesity is an increasing problem worldwide. In general, people eat more and exercise less, with a lot of individuals not meeting World Health Organization recommendation of 150-300 minutes of moderate-intensity physical activity or 75-150 minutes of vigorous-intensity physical activity weekly. At times, individuals may face barriers which prevent or inhibit their ability to participate in leisure-time physical activity (LTPA), exercise completed in one’s free time. These barriers are known as leisure constraints and are comprised of three categories: intrapersonal, feelings and psychological aspects; interpersonal, relationship aspects; and structural, physical aspects. Body image, the perception individuals have about their bodies and …


Optimization Of Transformation And Regeneration In The Sunflower (Helianthus Annuus), Sara M. Kilar Jan 2025

Optimization Of Transformation And Regeneration In The Sunflower (Helianthus Annuus), Sara M. Kilar

Masters Theses

In addition to their value as prized ornamentals, sunflowers are important crop species due to the nutritional value and high oil content of the seeds. Sunflower oil is a significant contributor to global seed oil production, which can be used as a food additive and cooking agent as well as a raw material for biodiesel production. While traditional breeding efforts have led to the development of many unique cultivars, genetically modified variations have proven more difficult due to the recalcitrance of sunflower plants to genetic transformation. As a result, facile and efficient transformation methods for sunflowers have the potential to …


Influence Of Thermophilic Biofilms On Proliferation Of Opportunistic Pathogens In Domestic Water Systems, Olajumoke Akinbami Jan 2025

Influence Of Thermophilic Biofilms On Proliferation Of Opportunistic Pathogens In Domestic Water Systems, Olajumoke Akinbami

Masters Theses

Domestic hot-water systems quietly grow biofilms under elevated temperatures that can serve as shelter to Opportunistic Premise Plumbing Pathogens. Although focus has been placed on microbes in domestic systems, there has been no clear conclusion on the mechanism of survival and persistence of opportunistic pathogens despite various disinfection strategies. In this study, we determined the distinction between showerhead and drip-flow biofilms at the whole-community level and analyzed the coexistence of thermotolerant organisms with opportunistic genera, such as Legionella. We sampled eight showerheads and simulated four biofilm samples in the laboratory using the drip-flow biofilm reactor.

We observed a strong …


2024 Celebration Of Scholarship, Creativity, And Engagement, Todd Bruns, Anna Nelson Oct 2024

2024 Celebration Of Scholarship, Creativity, And Engagement, Todd Bruns, Anna Nelson

Programs

Annually, Eastern Illinois University honors faculty achievements in the areas of research, creativity, and engagement. The list of faculty accomplishments is truly impressive, reflective of an engaged faculty committed to excellence and the ongoing pursuit and creation of knowledge. Many of these accomplishments occurred in collaboration with our students, who serve as co-authors, research assistants and partners in bringing these activities to fruition. In some cases, these professional ambitions transpired over many years and are a testament to persistence and tenacity. We extend a sincere message of congratulations for this diverse range of achievements, which bring distinction to our departments, …


Prevalence Of Insecticide Resistance Gene In Aedes Albopictus Population In Illinois Regions, Merylann Ochieng Jan 2024

Prevalence Of Insecticide Resistance Gene In Aedes Albopictus Population In Illinois Regions, Merylann Ochieng

2024 Awards for Excellence in Student Research and Creative Activity - Documents

Mosquitoes are vectors known to transmit many pathogens and parasites that cause serious human diseases such as Malaria, Dengue, Yellow and Chikungunya fever, Encephalitis, and Filariasis (Becker et al., 2010) (Franklinos et al., 2019). Aedes albopictus specie transmits Zika, Chikungunya, yellow Fever and Rift valley fever viruses (Leta et al., 2018). Aedes species are geographically invading new areas due to globalization and environmental changes (Lwande et al., 2020). Temperature increase, severe rainfall, and high humidity encourage diseases to spread to new areas where new vectors may act as carriers (Näslund et al., 2021). Aedes albopictus are expanding further along the …


The Impact Of Fish Intake (Primarily The Omega-3 Fatty Acid Concentration) On Cognitive Decline: A Systematic Review, Emma Martin Jan 2024

The Impact Of Fish Intake (Primarily The Omega-3 Fatty Acid Concentration) On Cognitive Decline: A Systematic Review, Emma Martin

2024 Awards for Excellence in Student Research and Creative Activity - Documents

The purpose of this systematic review is to explore the effect of fish intake on cognitive decline. Previous studies on fish intake and cognitive decline have produced both supportive results and results that yield ineffectiveness. This review focuses primarily on omega 3 concentration from fish intake and its influence on mild cognitive impairment. Using several search engines, studies were selected by searching “fish intake and cognitive decline”. Inclusion criteria consisted of individuals diagnosed with MCI, intervention of fish intake, and omega 3 fatty acid concentration and cognitive function exams conducted at baseline and throughout the study. Nine out of the …


Estimating Daily Growth Of Age-0 Channel Catfish In A Free-Flowing, Commercially Exploited Midwestern River, Kaleb B. Wood Jan 2024

Estimating Daily Growth Of Age-0 Channel Catfish In A Free-Flowing, Commercially Exploited Midwestern River, Kaleb B. Wood

Masters Theses

The larval stage in fishes is an important stage in the life history, as their survival dictates the ability of the population to sustain itself as an adult population. While there is a plethora of information available for juvenile and adult life stages of Channel Catfish (Ictalurus punctatus), very little is understood about their larval life stage. This study sought to determine overall density, growth rates, and size structure of cohorts of age-0 Channel Catfish during a study year on the Wabash River. In 2018, I sampled age-0 Channel Catfish using a benthic trawl from May to September. …


Influence Of Seeding Strategy On Insect Communities Within Utility Lands, Kylee E. Nissen Jan 2024

Influence Of Seeding Strategy On Insect Communities Within Utility Lands, Kylee E. Nissen

Masters Theses

Pollinator populations are in decline globally due to anthropogenic changes, particularly in areas affected by agricultural expansion and urbanization. Utility lands - managed to prevent tall vegetation encroachment - represent an opportunity to establish native grasslands and support pollinator communities. This study investigates how different seeding strategies used by utility companies influence pollinator community composition within a 15-acre electric right-of-way (ROW) outside St. Louis, Illinois. Despite the logical benefits of native seeding to pollinators, utility companies are often hesitant to do this, often favoring seeding to turf. Seeded in late 2021, the site incorporates randomized plots with turfgrass seeding (no …


Using Community Science To Monitor Eastern Box Turtles And Engage Public In Conservation, Sarah Zoppa Jan 2024

Using Community Science To Monitor Eastern Box Turtles And Engage Public In Conservation, Sarah Zoppa

Masters Theses

The eastern box turtle (Terrapene carolina carolina; EBT) is a charismatic species with populations in decline due to habitat loss, disease, and other anthropogenic factors. However, due to their cryptic nature, studying an EBT population can be a difficult and intensive process. To investigate EBT populations in Coles County, Illinois, we used a combination of community science and computer-based pattern analysis to develop an approach to tracking individual turtles. Citizen volunteers submitted photos of encountered eastern box turtle carapaces for shell pattern analysis along with GPS coordinates of the encounter. We have received 220 submissions from 47 community volunteers. …


Nocturnal Behavior Of Communally Roosting American Crows (Corvus Brachyrhynchos), Caitlin M. Mrowiec Jan 2024

Nocturnal Behavior Of Communally Roosting American Crows (Corvus Brachyrhynchos), Caitlin M. Mrowiec

Masters Theses

During the winter months in Charleston, Illinois, thousands of American crows (Corvus brachyrhynchos) gather together each night to roost. While aspects of this impressive sight have been studied elsewhere, there is very limited information on how crows behave at night. Additionally, the mechanics and origins of communal roosting are not especially well understood, and the behaviors involved are not always consistently described. This study sought to provide a more complete assessment of crow communal roosting after sunset. I collected data between 2018 and 2020 by locating communal roosts and conducting continuous observations for 4 hours after sunset, with additional checks …


Investigating Morphological And Molecular Identifiers For Culex Restuans And Culex Pipiens From Central Illinois, Savannah L. Ettien Jan 2024

Investigating Morphological And Molecular Identifiers For Culex Restuans And Culex Pipiens From Central Illinois, Savannah L. Ettien

Masters Theses

Some of the most prominent vectors for West Nile Virus are mosquitoes of the genus Culex. As a result, efforts have been made to monitor the spread of Culex species as climate change has allowed them to travel to places that they never inhabited before. Since Cx. restuans is a less prevalent vector, most studies for prevention focus on Cx. pipiens, which is the primary vector for the spread of West Nile Virus in the Midwestern United States, especially in the late summer and early fall seasons. As both species share many morphological characteristics and are present at …