Life Sciences Commons^™

Hematopoiesis Of Immature Myeloid Dendritic Cells In Stroma-Dependent Spleen Long-Term Cultures Occurs Independently Of Nf-Kb/Relb Function, Jonathan Tan, Keping Ni, Fei Le, Helen O'Neill

Jonathan Tan

Objective The nuclear factor-κB (NF-KB)/RelB transcription factor plays an essential role in development of some dendritic cell (DC) subsets in mice. In this laboratory, immature myeloid DC are produced in vitro in a stroma-dependent murine spleen long-term culture (LTC) system. In LTC, DC differentiate from hematopoietic progenitors maintained within the stromal cell matrix. Expression and function of RelB in development of LTC-DC has been investigated, with a view to assessing the relationship between DC produced in this system and other known subsets of DC. Materials and Methods RelB expression by LTC-DC was confirmed by detection of protein by Western blotting, …

Concise Review: Dendritic Cell Development In The Context Of The Spleen Microenvironment, Jonathan Tan, Helen O'Neill

Jonathan Tan

The dendritic cell (DC) population in spleen comprises a mixture of cells including endogenous DC progenitors, DC precursors migrating in from blood and bone marrow, and DC in different states of differentiation and activation. A role for different microenvironments in supporting the dynamic development of murine DC of different types or lineages is considered here. Recent evidence for production of DC dependent on splenic stromal cells is reviewed in the light of evidence that cell production is dependent on cells comprising an endothelial niche in spleen. The possibility that self-renewing progenitors in spleen give rise to DC with tolerogenic or …

A Guide To The Natural History Of The Central Platte Valley Of Nebraska, Paul A. Johnsgard

Papers in Ornithology

This book has been written in the hope that visitors to the Platte Valley may gain a greater appreciation for it through learning some of its animals and plants in addition to the Platte Valley's star spring attraction, its sandhill cranes. Besides the cranes, we have a world-class migration of geese and ducks, a slightly later migration of shorebirds, including what is probably the buff-breasted sandpiper's most important spring staging area between its South American wintering grounds and its arctic breeding grounds. Nebraska also has what may be the largest surviving population of greater prairie-chickens of any state, and an …

Lake Mead National Recreation Area Sensitive Wildlife Species Monitoring And Analysis: Quarterly Progress Report, Period Ending December 31, 2007, Margaret N. Rees

Wildlife Monitoring

Project 1. Relict Leopard Frog Monitoring, Management, and Research

• Fall surveys completed at all natural and translocation sites.
• Coordination for a potential translocation site on BLM lands in the Gold Butte area ongoing – the aim is that this site will be ready for translocations in 2008.
• Coordination ongoing for evaluation of a translocation site on BLM lands in the Black Mountains, AZ – the plan is to complete compliance in 2008.
• Coordination and assistance provided to UNLV research efforts on habitat improvements at Blue Point, Rogers, and Pupfish Refuge springs.
• Relict Leopard Frog Conservation Team meeting held in December. …

Lake Mead National Recreation Area Vegetation Monitoring And Analysis: Quarterly Progress Report, October 1, 2007 To December 31, 2007, Margaret N. Rees

Vegetation Monitoring

Executive Summary

• The Weed Sentry program surveyed more than 750 miles of federal lands in Clark County for invasive, exotic plant species.
• Weed Sentry also removed more than 600,000 individual invasive plants from federal
  lands. These removals represent pro-active efforts that may have forestalled large
  infestations difficult and costly to eradicate.
• In response to a request by the manager of the Fish and Wildlife Service Desert National
  Wildlife Refuge, a major effort of surveying springs for plant community composition
  and invasive plants provided unique knowledge services by the Weed Sentry program.
  No such baseline information existed for the Sheep Range. …

Joint Fire Science Program – Lake Mead National Recreation Area: Revegetating Burned Arid Lands: Identifying Successful Native Species Using Trait And Competition Analysis, Quarterly Progress Report, October 1, 2007 To December 31, 2007, Margaret N. Rees

Fire Science

The task agreement was awarded to UNLV on October 1, 2007. This report covers the period October 1, 2007 to December 31, 2007. The following activities have been conducted toward meeting or exceeding deliverables in the statement of work: hiring and project start and experimental set-up

Limnological Assistance For The Lake Mead National Recreation Area In Meeting The Challenge Of The Water 2025 Initiative: Quarterly Progress Report, Period Ending: December 31, 2007, Margaret N. Rees

Limnological Studies

• The limnologist position has been announced.
• Project 1: Review of the draft Boulder Basin Adaptive Management Plan is underway.
• Project 2: Development of a white paper expressing the joint needs of the entities involved in Water 2025 projects is being coordinated.
• Project 3: A bibliographic reference spreadsheet organizing literature citations related to quagga and zebra mussel research has been completed.
• Project 4: A comprehensive literature search of Lake Mead limnology is well underway; an Access database has been designed to organize reference citations; and a special collections library at UNLV is being established to house original rare documents and other …

Interagency Science And Research: Quarterly Progress Report, Period Ending December 31, 2007, Margaret N. Rees

Interagency Science and Research Strategy

• Meetings were held with the SNAP Recreation Team and the SNAP Cultural Resources Team to request assistance in the development of the science strategy. Draft versions of interagency goals, subgoals, science questions, and associated tasks were given to each team for review and input.
• Plans were prepared for the peer review of science proposals submitted for consideration as Conservations Initiatives in SNPLMA Round 9.
• Proceedings of the natural resources management workshop held on September 13 were completed and sent to the Science & Research Team for review by members.
• An important focus of the Science and Research Team was the …

Seasonal Appearance And Monitoring Of Invasive Species In The Great Bay Estuarine System, Larry G. Harris, Jennifer A. Dijkstra

PREP Reports & Publications

The University of New Hampshire Zoology Department reports on a study designed to synthesize existing data on invasive species in the estuary and the surrounding area, compare succession between two panel studies (1979 to 1982 and 2003 to 2006), seasonally monitor invasive species in the Great Bay Estuary, and identify predators of invasive species. Researchers identified species most likely to invade the Great Bay Estuary, analyzed succession between two long-term panel studies separated by approximately 25 years, collected presence/absence and abundance data of invasive species at four sites within the Great Bay Estuarine System and identified potential predators of invasive …

Predicting The Success Of Invasive Species In The Great Bay Estuarine Researve, Jennifer A. Dijkstra, Larry G. Harris

PREP Reports & Publications

The University of New Hampshire Zoology Department reports on a study designed to continue monitoring the distribution of invasive species in the Great Bay Estuary and to carry out laboratory experiments designed to test the effects of salinity on ascidian mortality and determine predators of ascidian species. Researchers collected presence/absence and abundance data of invasive species at four sites within the Great Bay Estuarine System. The report gives a brief description of the results of the monitoring program to compare results obtained from 2006 to 2007 and to assess the response of ascidians to varying salinity and predators. This report …

Buffer Projects In The Town Of Wakefield And City Of Somersworth, Julie Labranche

PREP Reports & Publications

Participants in the Wakefield Buffer Project include SRPC staff, members of the Acton Wakefield Watershed Alliance (AWWA), local board and commission members, and the Wakefield Town Planner, Kathy Menici. AWWA members included: Linda Schier, Carol Lafond, Marge Kimball and Adam Soukimas. Town Staff included Joe Fluet (Planning board Chair) and Nancy Spencer Smith (Conservation Commission). The goals of AWWA are to educate and inform shoreland property owners about the harmful effects of erosion and uncontrolled stormwater runoff on water quality of its lakes and ponds. AWWA focuses their on- the-ground efforts to repair, restore and preserve healthy functioning buffers and …

A Guide To The Tallgrass Prairies Of Eastern Nebraska And Adjacent States, Paul A. Johnsgard

Papers in Ornithology

Contents

Part 1: Ecology of the Tallgrass Prairie

Part 2: Plants of the Tallgrass Prairies:
Checklist of Prairie & Grassland Plants of Nebraska; Relative Frequencies of Grasses, Spring Creek and Nine-mile Prairies; Twenty Most Abundant Fall Forbs, Spring Creek and Nine-mile Prairies; Typical Shrubs, Forbs, Grasses & Sedges of Eastern Nebraska Tallgrass Prairies; English: Latin Name Equivalents of 370 Tallgrass Prairie Plants; Latin: English Name Equivalents of 370 Tallgrass Prairie Plants Identification Keys to Some Common Nebraska Grassland Forbs

Part 3: Animals of the Tallgrass Prairies
Greater Prairie-chickens and Native Prairies; Seasonal Checklist of Nebraska Tallgrass Ecoregion Birds (Gage County); …

The Birds Of Nebraska, Revised Edition 2007, Paul A. Johnsgard

Papers in Ornithology

Persons living in Nebraska often feel that they are living in a cultural wasteland; its citizenry preoccupied with violent sports such as hunting and football. Yet many are unaware that they are actually residing in one of the prime locations in the entire world for observing and enjoying some of the most aesthetically appealing of all the world's biological attractions. The area around Kingsley Dam and Lake McConaughy, for example, is known to have attracted more than 300 bird species, including 104 breeders (plus 17 probable breeders) making it the third-most species-rich bird location in the entire U.S.A. (after Laguna …

Pioneering Lobster Aquaculture In Rhode Island, Michael A. Rice

Michael A Rice

Regional Body Composition In College-Aged Caucasians From Anthropometric Measures, Robert T. Davidson, Cameron B. Ritchie

Faculty Publications

Quantitating fat and lean tissue in isolated body regions may be helpful or required in obesity and health-outcomes research. However, current methods of regional body composition measurement require specialized, expensive equipment such as that used in computed tomography or dual energy x-ray absorptiometry (DEXA). Simple body size or circumference measurement relationships to body composition have been developed but are limited to whole-body applications. We investigated relationships between body size measurements and regional body composition. Methods Using DEXA technology we determined the fat and lean tissue composition for six regions of the body in predominantly Caucasian, college-aged men (n = 32) …

An Optical Model For Heat And Salt Budget Estimation For Shallow Seas, Hari Warrior, Kendall L. Carder

Marine Science Faculty Publications

The effects of the underwater light field on heat-budget calculations for shallow waters are developed and applied for the region of Bahamas. Most of the general circulation models use a simplified heat budget scheme based on Jerlov water types, and do not account for optical bottom effects. by optical bottom effect, we mean the bottom absorption and reflection of the short-wave radiation, which in turn affects the thermal stratification and heat exchange with the atmosphere. In this paper, this optical bottom effect is added to a 3D turbulence model (a 1D model called GOTM is coupled to a 3D model …

Effect Of The Co2 Grinding On Modified Atmosphere And Color Shelf Life Of Ground Beef, Sofiane Ghris

All Theses

Beef ground under air and carbon dioxide (CO2) conditions were evaluated for their storage stability at 2 ± 1¼C starting after 2 days in the dark to simulate transportation, followed by 7 days in lighted display by measuring color (CIE L*, a*, b*, the hue and chroma), gas headspace, microbiological content, and pigment content. AC (ground in air then packaged in 100% CO2 MAP) and CC (ground in CO2 then packaged in 100% CO2 MAP) treatments showed the highest a* and chroma values from day 3 to 9 compared with AV (ground in air then vacuum packaged) and CV (ground …

Sara Of Staphylococcus Aureus Binds To The Sara Promoter To Regulate Gene Expression, Ambrose L. Cheung, Koren Nishina, Adhar C. Manna

Dartmouth Scholarship

The 375-bp sarA open reading frame is driven by three promoters, P1, P3, and P2. Using gel shift and DNase I footprinting assays, we found that SarA binds to two 26-bp sequences and one 31-bp sequence within the P1 and P3 promoters, respectively. Together with the results of transcription analyses, our data indicate that SarA binds to its own promoter to down-regulate sarA expression.

Development And Evaluation Of Instruments To , Patricia Michaud

All Theses

The objectives of this study are to provide evidence for and demonstrate the processes used to develop and test tools to measure the effectiveness of a culinary and nutrition education program. This study initially involved a review of literature related to the environmental conditions for and components of nutrition education interventions featuring hands-on cooking skill activities. Based on this literature examination, the Cooking Attitude, Cooking Behavior, Produce Consumption Self-Efficacy, Cooking Self-Efficacy, Self-Efficacy for Using Basic Cooking Techniques, and Self-Efficacy for Using Fruits, Vegetables and Seasonings scales and the Knowledge of Cooking Terms and Techniques evaluation were developed. The Availability and …

A High Resolution Satellite-Only Grace-Based Mean Dynamic Topography Of The South Atlantic Ocean, Marcio L. Vianna, Viviane V. Menezes, D. Chambers

Marine Science Faculty Publications

A new filtering method based on Singular Spectrum Analysis has been devised to extract high resolution (0.25° × 0.25° grid) satellite-only Mean Dynamic Ocean Topography(MDT) constructed by differencing of the GGM02 GRACE Gravity Model from the GSFCMSS00 Mean Sea Surface. This data-adaptive interpolation-type filter is adequate for the finite domain MDT processing since it minimizes smoothing and does not lose boundary points. It is applied to the computation of two new MDTs and MDT-derived mean geostrophic circulation maps of the South Atlantic region from 15°N to 50°S. Advantages of these new maps are clarified when contrasted with the Rio-2005, Chambers-Zlotnicki-2004 …

Natal Dispersal And New Group Formation In Capybaras (Hydrochoerus Hydrochaeris) In A Seasonally Flooded Savanna Of Venezuela, Elizabeth Rae Congdon

Dissertations

This study examines natal dispersal and new group formation in capybaras (Hydrochoerus hydrochaeris) in a seasonally flooded savanna in Venezuela. The first section describes a novel approach to the study of dispersal that could be applied to many taxa. Dispersal is considered in three stages (emigration, transience, and immigration) and its proximate and ultimate mechanisms are clearly differentiated. The second chapter describes dispersal behavior in capybaras, including which individuals disperse, when, and to where. In the third chapter, I evaluate dispersal in capybaras with respect to social subordination and social cohesion hypotheses. In this population, some support was …

Solution Structure Of Selenoprotein W And Nmr Analysis Of Its Interaction With 14-3-3 Proteins, Finn L. Aachmann, Dmitri E. Fomenko, Alice Soragni, Vadim N. Gladyshev, Alexander Dikiy

Vadim Gladyshev Publications

Selenium is a trace element with significant biomedical potential. It is essential in mammals due to its occurrence in several proteins in the form of selenocysteine (Sec). One of the most abundant mamma¬lian Sec-containing proteins is selenoprotein W (SelW). This protein of unknown function has a broad expression pattern and contains a candidate CXXU (where U represents Sec) redox motif. Here, we re¬port the solution structure of the Sec¹³ →Cys variant of mouse SelW determined through high resolution NMR spectroscopy. The protein has a thioredoxin-like fold with the CXXU motif located in an exposed loop similarly to the redox-active …

Gene Response Profiles For Daphnia Pulex Exposed To The Environmental Stressor Cadmium Reveals Novel Crustacean Metallothioneins, Joseph R. Shaw, John K. Colbourne, Jennifer C. Davey, Stephen P. Glaholt, Thomas H. Hampton, Celia Y. Chen, Carol L. Folt, Joshua W. Hamilton

Dartmouth Scholarship

Genomic research tools such as microarrays are proving to be important resources to study the complex regulation of genes that respond to environmental perturbations. A first generation cDNA microarray was developed for the environmental indicator species Daphnia pulex, to identify genes whose regulation is modulated following exposure to the metal stressor cadmium. Our experiments revealed interesting changes in gene transcription that suggest their biological roles and their potentially toxicological features in responding to this important environmental contaminant.

Arabian Sea Response To Monsoon Variations, Raghu Murtugudde, Richard Seager, Prasad Thoppil

Faculty Publications

This study aims to quantify the impact of strong monsoons on the mixed layer heat budget in the Arabian Sea by contrasting forced ocean general circulation model simulations with composite strong and weak monsoon winds. Strong (weak) monsoons are defined as years with zonal component of the Somali Jet being greater (smaller) by more than a standard deviation of the long-term mean of the National Centers for Environmental Prediction reanalysis winds. Coastal upwelling is shown to be demonstrably stronger for strong monsoons leading to significant surface cooling, shallower thermoclines, and deeper mixed layers. A coupled ecosystem model shows that surface …

Northeast Research Station Watertown, South Dakota Annual Progress Report, 2007, Agricultural Experiment Station, Plant Science Department

Agricultural Experiment Station and Research Farm Annual Reports

This is the 2007 annual progress report for the Northeast Research Station in Watertown, South Dakota. This report is issued by the South Dakota Agricultural Experiment Station and the South Dakota State University Plant Science Department. This report includes weather data, yield comparisons, crop performance trials, corn trials, soybean trials, winter wheat performance testing, Barley Foliar information, weed and pest control, herbicide demonstrations, canola and flax variety trials and more.

Synthesis, Characterization And Antimicrobial Studies Of Schiff Base Complexes Of Tripositive And Dipositive Transition Metal Complexes., Nagda El Kholi

Al-Azhar Bulletin of Science

A novel tetradentate Schiff base (I) synthesized from sulfametrole [N1-(4-methoxy-1,2,5-thiadiazole-3-y1)sulfanilamide] and salciyldelyde forms stable complexes with transition metal ions such as Fe(III), Fe(II), Co(II), Ni(II), Cu(II), Zn(II), Cd(II) and UO2(II) in absolute ethanol. Elemental analyses, IR, 1HNMR, electronic spectra, magnetic moment, mass spectra and thermal analysis (TGA) were used to confirm the structures. From the elemental analyses data, the complexes were proposed to have the general formulae [M2Cl3(I)(H2O)5].YH2O (Where M=Co(II),Ni(II),Cu(II), Zn(II) and Cd(II), Y=0-3], [Fe2Cl5(I)(H2O)3]: 2H2O, (FeSO4)2 (I)(H2O)4]:2H2O and [(UO2)2 (NO3)3 (I) (H2O)]:2H2O. The molar conductance data reveal that all the metal complexes were non-electrolytes. The IR, 1HNMR, spectra show …

Analyzing In Situ Gene Expression In The Mouse Brain With Image Registration, Feature Extraction And Block Clustering, Manjunatha Jagalur, Chris Pal, Erik G. Learned-Miller, R R Thomas Zoeller, David Kulp

Erik G Learned-Miller

Background: Many important high throughput projects use in situ hybridization and may require the analysis of images of spatial cross sections of organisms taken with cellular level resolution. Projects creating gene expression atlases at unprecedented scales for the embryonic fruit fly as well as the embryonic and adult mouse already involve the analysis of hundreds of thousands of high resolution experimental images mapping mRNA expression patterns. Challenges include accurate registration of highly deformed tissues, associating cells with known anatomical regions, and identifying groups of genes whose expression is coordinately regulated with respect to both concentration and spatial location. Solutions to …

Preparation Of Divalent And Trivalent Antigens For Immunogical Studies On Degranulation Of Mast Cells And Preparation Of Ceragenins For Antiviral Studies Against Vaccinia Virus., Dianliang Geng

Theses and Dissertations

CHAPTER 1

Aggregation of receptors for IgE (Fc RI) causes mast cells and basophils to release preformed contents of granules, including histamine and a variety of enzymes. This process, called degranulation plays a central role in allergic reactions. Methods to study this process are to create multivalent ligands which can interact with the receptors and, in turn, lead to aggregation of the receptors. We prepared a series of fluorophore-labeled divalent and trivalent antigens to study the degranulation of mast cells. Trivalent antigens proved to be much better stimulators for degranulation of mast cells than divalent antigens. These results indicate that …

Pyridinium Bisretinoids: Synthesis And Photoactivated Cytotoxicity, Junping Gao

Theses and Dissertations

This thesis discusses pyridinium bisretinoid compounds (PBRs), which were prepared for two purposes: 1) to use them as standards for detection of novel fluorophores in human RPE cells, which may be involved in age-related macular degeneration (AMD), and 2) to use them in the development of a targeted and triggered drug delivery system for cancer therapy. We prepared a selection of PBRs using a one-pot biomimetic method; synthesis, mechanisms for formation, and characterization of these compounds is described. We also explored the photoreactivity of three novel PBR compounds and found that these PBRs form oxidation products under blue-light irradiation. The …

A Biomechanical Analysis Of Male And Female Intermediate Hurdlers And Steeplechasers, Laurence R. Bollschweiler

Theses and Dissertations

In the sport of track and field, proper hurdling technique is a complicated combination of various running and jumping kinematics. With most research having been done on sprint hurdling, there is a growing need for research on hurdling events of different lengths. The intermediate hurdles (IH) and the steeplechase (SC) are two events where there are a number of differences in hurdling technique. This study compared the differences in hurdling technique between events (IH and SC) as well as the differences in technique between genders. Subjects for this study consisted of 20 elite intermediate hurdlers (10 male, 10 female) and …