Life Sciences Commons^™

Dexterous Manipulation Capabilities Are Associated With Change In Discharge Rate Properties Of Motor Neurons With Age, Mukta N. Joshi

Theses and Dissertations

Aging is accompanied by declines in manual dexterity and fine motor control. The purpose of this research was to compare hand motor control in young and older adults and examine the neuromuscular mechanisms responsible for enabling these interactions. We test force variability during isometric and dynamic contractions, manual dexterity and track motor unit activity to identify the neuromuscular mechanisms responsible for changes in dexterity with age. 26 older adults (66-86 years) and 28 young adults (19 – 38 years) participated in the study. Research participants performed force matching tasks during index finger abduction, precision pinch, static pressing and hybrid force/ …

Exploring Mesolimbic Circuitry Modulation By Opiates, Interleukin-10, And Psychostimulants, Joakim W. Ronström

Theses and Dissertations

The mesolimbic dopamine (DA) system originates in the ventral tegmental area (VTA) and projects to the nucleus accumbens (NAc) and other areas including the basolateral amygdala (BLA), prefrontal cortex, and the hippocampus. Drug use induces reward and leads to dysregulation in these brain areas and eventually to substance use disorders (SUDs). Chapter 1 introduces the mesolimbic DA system and its relationship to drug use and their relevance to each chapter. Chapter 2 explores opioid effects on BLA circuitry which is known to play a role in the emotional response including anxiety and stress in SUDs. We showed that morphine induced …

Characterization Of Cellular Metabolism Regulation By The Transcription Factor Centromere Binding Factor 1 (Cbf1), Spencer Ellsworth

Theses and Dissertations

Centromere binding factor 1 (Cbf1) is a transcription factor that controls the transcription of many genes involved in cellular respiration and lipid biogenesis and, as such, has been associated with hypolipidemia in humans. It is a known substrate for PAS kinase, which phosphorylates Cbf1 and alters its activity. Our hypothesis is that this phosphorylation affects the genes it regulates and the DNA motifs it binds to, perhaps due to different transcription complexes being formed. In this study, we conduct a chromatin immunoprecipitation in Saccharomyces cerevisiae to determine what genes and DNA motifs Cbf1 binds to in its wild type versus …

The Effects Of Diesel Exhaust Particle Exposure On Adipos Mitochondrial Bioenergetics And Inflammation, Cali Elizabeth Warren

Theses and Dissertations

Fine particulate matter (PM2.5) constitutes a significant component of ambient air pollution that has been implicated in the pathogenesis of metabolic disorders, including insulin resistance and type 2 diabetes. Among PM2.5 constituents, diesel exhaust particles (DEP) are prevalent particulates that infiltrate the bloodstream to drive systemic pathologies. The purpose of this study was to characterize the metabolic response of adipose tissue to DEP. We aimed to provide a comprehensive understanding by exploring mitochondrial bioenergetics, characterizing the inflammatory marker profile, including adipokines, and conducting a detailed histological analysis of adipocytes to provide valuable insights to the evolving understanding of the intricate …

Applicability Of Using Bio-Receptive Concrete For Building Facades In Egypt, Gina Roupheil

Theses and Dissertations

The significant increase in carbon dioxide emissions caused by the construction industry is detrimental to our planet. This is rapidly increasing with the urbanization of cities that is gradually taking its toll on the available green spaces, which help in balancing such emissions. Recently, researchers have been trying to make use of bio-receptivity to create biomaterial systems that could be spread on building envelopes and support the growth of small plant species and microorganisms to establish on. Out of those materials, comes the concrete as a promising material for bio-colonization.

Within this context, this study aims at investigating the applicability …

Integrative Genomic Insights Into Coral Resilience: Adaptive And Acclimatory Responses To Seasonal Environmental Shifts, Tasnim Ghanim

Theses and Dissertations

Coral reefs, critical to marine biodiversity and coastal protection, face escalating threats from climate change-related phenomena such as ocean warming. This warming is leading to widespread thermal stress that contributes to coral bleaching and infectious disease in corals, leading to the disruption of marine ecosystems and the economies that depend on them. Focusing on the brain coral Platygyra daedalea, known for its thermal resilience in the Persian Arabian Gulf—a region that exemplifies the extreme stressors of climate change—our study aims to dissect the genetic, transcriptomic, and microbiological underpinnings of coral survival in high-temperature environments predicted for the end of …

Impairment Of The Glial Phagolysosomal System Drives Prion-Like Propagation Of Huntington’S Disease, Graham Davis

Theses and Dissertations

The ability of glia to tightly regulate neuronal health and homeostasis in the CNS is conserved across species. Yet, despite the ability to degrade protein aggregates, glia are vulnerable to the accumulation of neurotoxic amyloid aggregates during neurodegenerative disease progress, and even exacerbate their spread. A developing narrative highlights glia as a double-edged sword in neurodegenerative diseases: initially capable of dynamically responding to amyloid aggregate-ladened dying neurons but also capable of inducing chronic inflammation and creating seeding-competent amyloid oligomers. Thus, uncovering the mechanisms that allow glia to control aggregate deposition while preventing the neurotoxic effects and seed generation is vital …

Stacking And Intersecting Nutrient 4rs On Potato, Samuel Hamilton Stapley

Theses and Dissertations

The 4Rs of nutrient management is a global agronomic initiative with an aim to improve the sustainability of major cropping systems and the environment. The objective for this project is to evaluate individual and stacked 4R management practices and how they intersect. A field trial near Grace, Idaho was conducted on potato (Solanum tuberosum L.) in 2020 and 2023. Nitrogen (N) fertilizer treatments included all combinations of two sources [urea or polymer coated urea (PCU)], two rates (82 or 100% of the recommended rate), and/or two timing/placements (all applied at emergence or 84% at emergence + 16% fertigation simulation) compared …

Exploring A Gene Panel For Parkinson’S Disease In An Egyptian Cohort, Asmaa Saeed Gabr

Theses and Dissertations

Parkinson’s disease is a highly heterogeneous disorder characterized by diverse neuropathological features, clinical presentations and progression patterns. In Egypt, Parkinson’s disease incidence rates lie outside the range reported elsewhere. The genetic background to the pathogenesis of Parkinson's disease has been postulated for a long time. However, Parkinson’s disease has never been systematically investigated in Egypt. This study aimed to explore genetic variants and interactions that are associated with the familial and sporadic forms of Parkinson's disease in an Egyptian cohort. This includes examining variants in PD-related genes, exploring the role of specific genes like MAPT and adjacent genomic regions, and …

The Role Of The Cdk8 Kinase Module In Maintaining Proteostasis, Stephen Willis

Theses and Dissertations

The underlying etiology of numerous disease states results from perturbations in the maintenance of cellular proteostasis. Carcinogenesis relies on these perturbations to foster uncontrolled cell growth and eventual metastases, while neurodegenerative diseases are a consequence of such perturbations. Control of these processes occurs at numerous molecular levels, commonly starting with transcription. A key transcriptional complex that is involved is the CDK8 Kinase Module (CKM). The CKM is conserved from yeast to man, forming a tetrameric complex consisting of MED12, MED13, CDK8, and CCNC. The CKM has not only been implicated in a variety of cancers but also in a spectrum …

Elastin-Like Polypeptide As A Model To Study Intrinsically Disordered Proteins, Sadegh Majdi

Theses and Dissertations

The elastin-like polypeptide (ELP) is a well-studied structural protein that is easily amenable to amino acid (AA) sequence modifications and has the potential to yield a wide variety of uses in bioengineering and biomedical applications. One unique property of ELP is the inclusion of intrinsically disordered domains (IDP) within the structure that allow for its diversity of physical properties. While it is generally understood that amino acid sequence dictates protein folding arrangements, the contributions of specific amino acid sequences to the intrinsic disorder of ELP has yet to be fully resolved. Therefore, identifying the contributions of specific amino acid sequences …

Mechanistic Analysis Of Four-Way Dna Junctions And Cytokine-Binding Aptamers For Therapeutic Interventions, Roaa S. Mahmoud

Theses and Dissertations

DNA is inherently dynamic and topologically diverse and can fold into many different structures. Besides the canonical Watson-Crick structure, other higher-order structures such as G-quadruplexes (G4), i-motifs (iM), and four-way DNA junctions are possible. Although these high-order DNA structures are known to form transiently, they are important due to the crucial roles they play in many cellular processes including DNA replication, recombination, and repair. Among these DNA structures, 4-way junctions (also known as Holliday junctions, HJ) which are formed during the repair of double-strand DNA breaks (DSBs) and interact with proteins have garnered significant attention due to their central role …

Investigating Ammonium Toxicity In The Performance Of Recirculating Periphytic Algal Wastewater Treatment Systems, Oluchuku Richie Ochonogor

Theses and Dissertations

Freshwater nutrient enrichment from wastewater facilities and other sources can lead to freshwater eutrophication, a threat to global aquatic ecosystems. Mechanical and chemical ways to curb this threat are either too expensive or not sustainable, and thus, not feasible. Compared to mechanical and other methods, sustainable, inexpensive biological methods (for example, algal treatment systems) have therefore been developed for the removal of excess nutrients from wastewater. By design, secondary waste treatment facilities (WWTF) remove organics and solids and lower oxygen demanding substances; however, they may not remove enough nutrients to protect freshwater ecosystems in all cases. While algae-based biological methods …

Effect Of Timing Of Energy Intake On Blood Glucose And Body Composition In Women With Overweight Or Class One Obesity, Michael Wade Smith

Theses and Dissertations

Overweight and obesity has increased in the U.S. Unfortunately, this increases the risk of metabolic dysfunction, such as diabetes. Meal timing has recently shown promise as viable options to minimize the risk of overweight or obesity and metabolic dysfunction. The primary purpose of this study, therefore, was to investigate the effect of timing of energy intake on blood glucose in females with overweight or class one obesity (BMI=27-35 kg/m2). This study utilized crossover design with two conditions counterbalanced in random order. Participants completed two 2-week 1500kcal dietary interventions: 1) a big breakfast diet or BB (50% of energy intake between …

Investigating The Impact Of Transcription On Mutation Rates, Sarah Patterson

Theses and Dissertations

tRNA genes are highly transcribed and perform one of the most fundamental cellular functions. Although a universal pattern observed across all three domains of life is that highly transcribed genes tend to evolve slowly, tRNA genes have been shown previously to evolve rapidly. This rapid sequence evolution could result from relaxed selection, increased mutation rate, or a combination of both. Here, we use mutation-accumulation line sequencing data to show that tRNA genes accumulate more mutations than other gene types. Our results indicate that this elevated mutation rate is a consequence of both elevated transcription-associated mutagenesis and a lack of transcription-coupled …

The Effects Of Intermittent Fasting On Female Athletes: A Systematic Review, Courtnie Scarbrough

Theses and Dissertations

The intermittent fasting diet has a historical past, but recently, it has regained popularity as a weight loss strategy for the general public. Athletes have also picked up this specialized diet in search of a competitive edge. There are ample studies testing athletes of all levels, but most do not include females or report sex-specific results. This systematic review aimed to identify current research on the effects of intermittent fasting in the understudied population of female athletes. Following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines, a comprehensive search was conducted in three databases, including PubMed, Cochrane, …

The Influence Of At-Planting Insecticide Treatments On Tobacco Thrips, (Thysanoptera:Thripidae), And Reniform Nematode, (Tylenchida:Hoplolaimidae), In Conventional And Thryvon Cotton Systems, Walker Brett Farmer

Theses and Dissertations

One of the objectives of this research was to evaluate the need for an additional imidacloprid seed treatment on ThryvOn^® (Bayer Crop Science^®, St. Louis, MO) cotton to effectively manage tobacco thrips, Frankliniella fusca (Hinds). While evaluating thrips densities and damage ratings, ThryvOn cotton continued to display reduced damage and thrips populations than non-ThryvOn. Thrips populations and damage ratings were consistently reduced when incorporating a higher rate of imidacloprid, but they were not reduced enough to justify the higher rates of imidacloprid. Another objective of this research was to evaluate the impact of aldicarb (AgLogic Chemical^{® …}

Habitat Utilization Of Marsh And Adjacent Submerged Landscape By Fish And Macroinvertebrates In A Gulf Of Mexico Tidal Oligohaline Environment, Tia Offner

Theses and Dissertations

Estuarine habitats are considered nursery habitats for fishes and invertebrates, but oligohaline environments are critically understudied. Using a seine net and fyke nets, we sampled Back Bay, Mississippi (USA), a low salinity estuary, once a month for a year to explore the temporal use of the marsh and adjacent submerged landscape by nekton species. We also looked at whether there is evidence of habitat preference in the most numerous nekton species. We used a novel habitat usage index (HUI) to compare seine and fyke net catches of our top 10 species, and evaluated catch size in relation to maturation size. …

Effects Of Supplementing Sources Of Zinc On Digestibility Parameters Of Beef Steers, Arminda Spikes James

Theses and Dissertations

Zinc (Zn) has numerous functions and life sustaining processes depend on the presence of Zn within the body. Zinc sulfate is a common form of Zn supplemented in the beef industry. Inorganic sulfate based minerals have been associated with negative rumen effects, mainly, decreased rumen fermentation and protozoa numbers. Our studies concluded no difference in digestibility of three different forms of Zn (zinc glycinate, zinc sulfate, and zinc hydroxychloride). Although overall digestibility did not change, there were specific mineral concentration differences after steers consumed concentrate treatment for 10 days. These differences implied that the mineral fed was consumed and absorbed …

Evaluating Selected Properties Of Underutilized Hardwood Species For Fabrication Of Cross-Laminated Timber Industrial Mats, Mercy Itunu Ogunruku

Theses and Dissertations

Softwood is more in demand than hardwood because it is used primarily in the US's largest wood-consuming industry, construction, resulting in increased importation of softwood annually to meet this demand. Hardwood, used for non-structural purposes like furniture and interior designs, is more abundant in US forests. However, some hardwood species are underutilized and undervalued. Cross-laminated timber (CLT) has increased the demand for softwood.

A study evaluated the mechanical and physical properties of three underutilized hardwood species (321 yellow poplar, 393 sweetgum, and 262 red oak specimens) for CLT industrial mat manufacturing. The results showed that red oak had a higher …

Insights Into The Challenges Posed By Climate Change And Land Competition To Brazil’S Midwest Pulpwood Market, Roberto Hajime Sant Anna Kimura

Theses and Dissertations

I investigated the effect of climate change in one of the few expanding markets in the world in Brazil. In the last decades the demand for pulpwood increased from zero to 11 million tons, leading to an expansion of 700 thousand hectares of Eucalyptus plantation. In 2024, a new mill will start operating increasing the consumption of wood fiber by 8.2 million tons (+49%). I used mathematical programming to investigate how different scenarios of productivity and land will affect the market. My results showed that around 946,000 hectares (+124%) of additional timberland will be necessary in the upcoming decades. The …

Towards Understanding The Interactions Between Ospreys And Human-Made Structures In The Tennessee River Valley, Natasha Karina Murphy

Theses and Dissertations

Raptor nests on human-built structures represent a significant source of conflict as they can result in bird mortality, fires, structure damage, service distribution, or power outages when falling nest materials or animals connect with energized conductors. Power companies, such as the Tennessee Valley Authority (TVA), wish to mitigate these conflicts to avoid service disruptions. In this dissertation, I present my work towards understanding and mitigating the interactions between Ospreys (Pandion haliaetus) and human-made structures. To achieve this, I explored multiple elements of conflict identification, monitoring, and basic ecology of the target species to better inform conflict mitigation. In Chapter I, …

Paternal Programming: The Role Of Seminal Plasma In Pregnancy Hemodynamics And Offspring Growth, Rebecca Michele Swanson

Theses and Dissertations

Seminal plasma is commonly known to serve as a transport medium for sperm as it moves through the female reproductive tract for fertilization, however, more recent evidence demonstrates seminal plasma induces an expansive inflammatory response in the uterus. Murine models have found this inflammatory response is important for clearing pathogens and poor-quality sperm, eliciting the secretion of cytokines, chemokines, and embryokines that aid in embryo attachment and growth, placental angiogenesis, and blunting maternal immunity to the embryo. However, there is minimal research on the impacts of seminal plasma uterine priming in bovine, and more specifically embryo growth, uterine blood flow, …

Characterizing Stress Granule Regulation By Pas Kinase, Ataxin-2 And Ptc6 And Investigating The Lifespan Of Covid-19 Virus On Currency, Colleen R. Newey

Theses and Dissertations

The protein Ataxin-2 is a known positive regulator of stress granules in humans, mice and yeast (known as yeast PBP1). Due to the role that stress granules play in diseases including Amyotrophic Lateral Sclerosis (ALS) and cancer, this thesis investigates the role of Ataxin-2 and its protein binding partners in stress granule development and its effects on various metabolic phenotypes of the cell. PAS kinase is a sensory protein kinase, conserved from yeast to man, which regulates respiration and lipid biosynthesis. Our lab discovered that PAS kinase phosphorylates and activates Ataxin-2 in yeast, and that PAS kinase overexpression enhances localization …

Soil Health In American Sports Fields, Miria C. Barnes

Theses and Dissertations

Healthy soils are essential for sustaining the world's ecosystems and maintaining human lifestyles. The adoption of biological, chemical, and physical analyses to assess soil health is a relatively new concept with a paucity of scientific work assessing how these metrics are affected by field management in urban systems Soil samples (n = 110) were collected from a diverse range of sports fields and, for comparative purposes, golf courses, farm fields, non-sport urban, undisturbed forest, and non-vegetated sandy soils. The samples were then analyzed using biological, chemical, and physical metrics to determine if there were significant differences between sport/golf venues and …

Use Of Microcalorimetry To Evaluate Hardening Reactions In Protein Bars During Accelerated Storage, Tiffany Rose Spackman

Theses and Dissertations

Protein bars have become a popular option among consumers to increase protein content in their diets. Since there is a large market for protein bars, many factors must be considered when creating a protein bar that both satisfies consumers and has a long shelf-life. Hardening and textural changes in protein bars are some of the most common modes of shelf-life failure in this product category. When the typical product creation timeline from formulation to launch can be as short as 3-6 months and with added pressure from executives to quickly launch another new product afterwards, product development scientists simply do …

Novel Fire And Herbivory Regime Impacts On Forest Regeneration And Plant Community Assembly, Devri A. Tanner

Theses and Dissertations

Human activities are increasing the occurrence of megafires that have the potential to alter the ecology of forest ecosystems. The objective of this study was to understand the impact of a 610 km2 megafire on patterns of forest regeneration and herbivory of three forest types (aspen/fir, oak/maple, and pinyon/juniper) within the burn scar. Sapling density, meristem removal, and height were measured across a transect network spanning the area of the burn scar over three years from 2019-2021. The network consisted of 17 burned/unburned transect pairs in adjacent burned/unburned forest stands (6 aspen/fir, 5 oak/maple, and 6 pinyon/juniper). Species that regenerated …

Aedes Aegypti Host Preference On Canines Versus Human Volatile Skin Compounds, Melissa Bernadette Rosalez

Theses and Dissertations

It is critical to understand how Aedes aegypti mosquitoes detect hosts through cues such as volatile skin compounds. In South Texas, evidence suggests that this mosquito is showing a feeding preference for canines over humans. This shift may impact the transmission of Dirofilaria immitis, canine heart worms, in local dog populations. Our study is set to explore Ae. Aegypti attraction to hosts using canine and human volatile compounds. Volatile skin compounds will be collected using a volatile collection system. Compounds will be analyzed using gas chromatography/mass spectrometry. It is anticipated that the main volatile skin compounds amongst humans are nonanal, …

Chemical Investigation Of Dichloromethane Extract Of Aloe Vera Peels: An Agricultural Waste, Nazmul Huda

Theses and Dissertations

Aloe barbadensis Miller, commonly called Aloe Vera, is a widely popular succulent plant species cultivated across subtropical regions worldwide from India to the Tex-Mex border. Besides its historical uses, Aloe juice has garnered attention as a potential remedy for various ailments, particularly in treating various skin conditions and facilitating wound recovery, including obesity, diabetes, hepatitis, Crohn's disease, and ulcerative colitis. Regrettably, the agricultural practice following sap extraction involves discarding Aloe vera peels, constituting agricultural waste. As part of our continuous research on extraction, isolation, separation, and spectral characterization of value-added chemicals present in waste botanicals herein, …

Effects Of Short-Term Pesticides Exposure On Swimming Behavior, Tissue Morphology, Oxidative-Nitrative Stress, Antioxidant Expression, And Cellular Apoptosis In Kidneys Of Goldfish, Esmirna Cantú

Theses and Dissertations

The prevalence of pesticides in the aquatic environment continues to increase due to anthropogenic activities and poses a threat to aquatic organisms. The aim of this study was to determine the short-term exposure (one-week) of pesticide mixtures (metolachlor, linuron, isoproturon, tebucanazole, alconifen, atrazine, pendimethalin; azinphos-methyl) on swimming behaviors, kidney morphology, and expression of nitrotyrosine protein (NTP), dinitrophenyl protein (DNP), superoxide dismutase (SOD), catalase (CAT), renin, and cellular apoptosis in goldfish (Carassius auratus). Results indicated that both low- and high-dose pesticide mixtures decreased the distance swimming behavior. Histological analysis showed pesticide exposure alters kidney morphology. DNP, NTP, and renin expressions, and …