Life Sciences Commons^™

Cover Pages For Tgle Vol. 54 Nos. 3 & 4

The Great Lakes Entomologist

Cover Pages for TGLE Vol. 54 Nos. 3 & 4

Full Issue For Tgle Vol. 54 Nos. 3 & 4

The Great Lakes Entomologist

Full issue for TGLE Vol. 54 Nos. 3 & 4

Smokejumper Obituary: Sleight, James Scott “Jim” (Mccall 1977), National Smokejumper Association

Smokejumper Obituaries

No abstract provided.

Optimization Of Sample Unit Size For Sampling Stink Bugs (Hemiptera: Pentatomidae) In Soybean, Arthur V. Ribeiro, Rafael Carlesso Aita, Daniela T. Pezzini, Christina D. Difonzo, Thomas Hunt, Janrt J. Knodel, Christian H. Krupke, Lia Marchi-Werle, Andrew P. Michel, Nicholas J. Seiter, Robert J. Wright, William D. Hutchison, Robert L. Koch

Department of Entomology: Faculty Publications

Cost-effective and reliable sampling procedures are crucial for integrated pest management. Sweep net sampling is commonly used for stink bugs (Hemiptera: Pentatomidae) in soybean, with sample size being the number of sets of sweeps, and sample unit size the number of sweeps in each set. Sample unit size has received little attention, but can affect sampling parameters. Here, two sample unit sizes (10 vs. 25 sweeps) were compared for the sampling of stink bug taxa. On average, sampling for stink bugs took 3.6 more minutes with the 25-sweep than with the 10-sweep sample unit size. Generally, estimates of the mean …

Targeted Osmotic Lysis: A Novel Approach To Targeted Cancer Therapies, Harry J. Gould, Dennis Paul

School of Medicine Faculty Publications

The conventional treatment of cancer has been based on the delivery of non-selective toxins and/or ionizing energy that affect both the cancer and normal tissues in the hope of destroying the offending disease before killing the patient. Unfortunately, resistance often develops to these treatments and patients experience severe, dose-limiting adverse effects that reduce treatment efficacy and compromise quality of life. Recent advances in our knowledge of the biology of tumor cells and their microenvironment, the recognition of surface proteins that are unique to specific cancers and essential to cell growth and survival and signaling pathways associate with invasion and metastasis …

Host-Pathogen Coevolution Between Tasmanian Devils (Sarcophilus Harrisii) And Devil Facial Tumor Disease, Dylan Garret Gallinson

USF Tampa Graduate Theses and Dissertations

Coevolution is a driving force of rapid evolution, yet the complexity of coevolutionary interactions has made it difficult to characterize the genomic basis of traits mediating such relationships. Coevolutionary dynamics are especially important in host-pathogen systems where the host and pathogen must constantly adapt to one another. The Tasmanian devil and its species-specific transmissible cancer, devil facial tumor disease (DFTD), provide the rare opportunity to study host-pathogen coevolution in a complex natural system. Extensive spatiotemporal devil sampling, high linkage disequilibrium in devils, and a large selective pressure imposed by DFTD facilitate a system tractable for study. Here, we characterized devil …

Ubjective Information And Survival In A Simulated Biological System, Tyler S. Barker, Massimiliano Pierobon, Peter J. Thomas

School of Computing: Faculty Publications

Information transmission and storage have gained traction as unifying concepts to characterize biological systems and their chances of survival and evolution at multiple scales. Despite the potential for an information-based mathematical framework to offer new insights into life processes and ways to interact with and control them, the main legacy is that of Shannon’s, where a purely syntactic characterization of information scores systems on the basis of their maximum information efficiency. The latter metrics seem not entirely suitable for biological systems, where transmission and storage of different pieces of information (carrying different semantics) can result in different chances of survival. …

Privacy-Preserving Logistic Regression With Secret Sharing, Ali Reza Ghavamipour, Fatih Turkmen, Xiaoqian Jiang

Faculty, Staff and Student Publications

BACKGROUND: Logistic regression (LR) is a widely used classification method for modeling binary outcomes in many medical data classification tasks. Researchers that collect and combine datasets from various data custodians and jurisdictions can greatly benefit from the increased statistical power to support their analysis goals. However, combining data from different sources creates serious privacy concerns that need to be addressed.

METHODS: In this paper, we propose two privacy-preserving protocols for performing logistic regression with the Newton-Raphson method in the estimation of parameters. Our proposals are based on secure Multi-Party Computation (MPC) and tailored to the honest majority and dishonest majority …

Fishing Without A Scale, Kaleb Miller, Luke Lemaitre

Student Scholarship - College of Business

Our study looks at different species of fish and uses their cross-length and volume to find correlations in weight among the different fish. The different type of fish that we compare include perch, pike, parkki, whitefish, bream, roach, and smelt. The dependent variable that we are using is weight. Once we predict the weight of the individual fish, we will also compare that weight to other fish species. The result of the data are that there is linearity, where the adjusted R squared is between 0.94 and 0.97, depending on our model. Therefore, 94 to 97 percent of the variability …

Understanding The Natural History Of Juvenile Amblyomma Maculatum In Southeastern Virginia, Christina Espada, Holly Gaff

College of Sciences Posters

Amblyomma maculatum, the Gulf Coast tick, is a species of increasing public health concern. Adult A. maculatum is a known vector of several pathogens including Rickettsia parkeri, the causative agent of Rickettsia parkeri rickettsiosis. Amblyomma maculatum has expanded northward from its historic range along the Gulf Coast, with populations reportedly establishing in southeastern Virginia in 2010. Recently established populations of A. maculatum tend to have higher R. parkeri infection prevalence compared to longer established populations. This pattern holds for all populations found so far in southeastern Virginia, with a prevalence of R. parkeri in about 60% of A. …

The Effects Of Temperature Changes On Bioluminescent Dinoflagellates (How Climate Change May Affect Our Ocean's Raves), Kayleigh X. Ambrose, Molly Fleming, Carla Caceres

PRECS student projects

Many species of Dinoflagellates are capable of producing bioluminescence. Most species will have an optimal temperature range for bioluminescence production. With sea surface temperatures expected to rise, we have designed an experiment to test the relationship between temperature and bioluminescence in Pyrocystis fusiformis.

Bacterial Metabolism Of Glucosinolates From Brassica: Association With Isothiocyanate Production, Stephanie Thomas, Jiaxuan Li, Michael J. Miller

PRECS student projects

Study Focus: To explore the alternative metabolic pathways of GSLs in bacteria, present on the surface of brassica vegetables.

Hypothesis: The alternative pathways used to metabolize GSLs in bacteria located on the surface of brassica vegetables, may contribute to the high variance of human gut GSL conversion to ITC.

Concentrating On Carbon Concentration In Algae, Chandra Davies, Steven Burgess

PRECS student projects

Carbon Concentrating Mechanisms Improve Photosynthesis Rates in Low-Carbon Environments. CCMs allow the aggregation of carbon near the site of rubisco, that way even small amounts of available carbon are being utilized.

Yes, this is important to you!

Albeit somewhat indirectly, but massively nonetheless! Algae are a driving force behind the global carbon cycle, they sequester CO2 in the oceans. Understanding the mechanisms behind the tiny marine alga O. tauri gives us a better understanding of a vital global process.

Polinators In Peril - Are Illinois Native Bees Getting Smaller?, Anthony Lamb, Tristan Barley, Adam Dolezal

PRECS student projects

Why it matters.

Pollination services provided by native bees are an important natural process, providing ~150 billion dollars globally in agricultural gains.

A bee’s body size is a trait that is an important indicator of the nutritional environment. A loss of habitat results in a loss of rich nutritional environments as flowers and resource become more fragmented and scarcer.

It has been discovered that a bee’s body size is a determinant of their foraging range. The smaller the bee, the less distance they can travel for food, therefor, the more susceptible they are to habitat loss and resource limitation.

Exploration Of Squash Plant Floral Volatiles Influencing Beetle Attraction, Olivia Barrett, Nicholas Seiter, Esther Ngumbi, Joseph Spencer, Sarah Hind

PRECS student projects

Flowers emit volatiles to attract pollinators, but these volatiles can also attract pest insects. Our study builds on earlier findings that showed that cucumber beetle attraction to squash flowers is mediated by volatile release and cucurbitacin accumulation (Theis, 2014, DOI: 10.3732/ajb.1400171). Of the three main domesticated squash species, only a few varieties of Cucurbita maxima had large quantities of floral volatiles and cucurbitacin (Andersen and Metcalf, 1987, doi: 10.1007/BF01020152); however, these studies were limited in the number of varieties that were examined. Here, we increased the number of C. maxima varieties examined, to identify patterns in floral volatile production, with …

How To Quantify Female Mate Preference In Threespine Stickleback, Kaithren Garcia, Megan Tucker, Meghan Maciejewski, Usan Dan, Alison M. Bell

PRECS student projects

Social behavior is diverse. For example, males from two stickleback ecotypes (whites and commons, Fig. 1) are highly divergent in courtship and parental care behavior [1]. Little is known about ecotypic differences in female behavior. In this study, we develop methods to quantify female preference in this system.

Intra- And Interspecific Variation In Tadpole Lateral Line Cells, Sarah Porth, Lisa Surber, Eva Fischer

PRECS student projects

Background

• The lateral line is a sensory system that detects motion and pressure in fish & larval amphibians.
• The sensory end organs are called neuromasts.
• A gosner stage describes the 46 stages of tadpole development

Hypothesis: We hypothesize that the number of neuromasts will vary more between species than within a species.

Stressed Out: Why Does Ancient Maize Thrive In High-Stress Conditions?, Alexander C. Lozano, Aaron Mleziva, Esther Ngumbi

PRECS student projects

Why is understanding stress response in maize so important?

• Maize, along with rice and wheat, provide at least 30% of calories to over 4.5 billion people
• Increases in flooding events has heavily impacted natural vegetation and crop production
• Combinatorial stresses, particularly flooding and herbivory, remain understudied for both modern and ancient verities of maize
• Ancient maize may be a superior genetic resource for flood-tolerant corn

Pressure Ulcer Prevention: A Quality Improvement Project, Noah Blevins, Graci Borders, Brett Bentley, Carly Crutchfield, Rachel Dillion, Jacob Dyer, Heleigh Eldridge, Taylor Emmons, Suzi White

2022 Celebration of Student Scholarship - Poster Presentations

A pressure ulcer is an injury that affects the underlying tissue and skin and is primarily caused by prolonged pressure on the skin. During a clinical rotation at a small rural teaching hospital, pressure ulcers were prevalent in an estimated 80% of patients in the intensive care unit (ICU) during a three-week period. Approximately $26.8 billion are spent every year in the United States treating pressure ulcers. The purpose of this quality improvement project is to provide staff education to ICU nurses in order to reduce and prevent the prevalence of pressure ulcers in patients. A thorough literature review was …

The Perception Of Minority Student-Athletes’ And Non-Athlete Students’ Learning Experience At A Predominately White Institution, William Wellman, Jelani Garvin, Jane Zhang, Steve Chen

2022 Celebration of Student Scholarship - Poster Presentations

This study examined mainly African-American and minority students' learning experience at a regional public university in Appalachia for the purpose of improving the existing academic support services and creating a safe diverse learning environment for racial minority students.

Frequency Of Genetic Variants At The Mc1r Locus In A Student Population, Emmalea K. Dowdy, Grace C. Stubblefield, David K. Peyton

2022 Celebration of Student Scholarship - Poster Presentations

The Melanocortin-1 Receptor gene (MC1R) encodes a protein that is associated with pigmentation in vertebrate animals. An extraordinary number of variations in this gene have arisen over time due to the importance of pigmentation in camouflage, photosensitivity, vitamin D production, and other evolutionary factors. Variations in the MC1R gene sequence became important to survival as humans migrated out of Africa and into cooler climates with less sun exposure, where lighter skin pigmentation (and therefore more vitamin D production) was key to survival. These genetic variations (alleles) continue to exist in modern humans. Recent research into the MC1R gene variations shows …

Research, Testing, And Validation Of Small Satellite Designs: Cubesats, Cansats, Pocketqubes, And Femtosatellites, Jason A. Combs, Jose L. Garcia

2022 Celebration of Student Scholarship - Poster Presentations

The focus of this project, through research and testing, was to prepare various elements of small satellite experiential education curriculum. Rather than be constrained to the expensive and long-term timelines of a larger satellites, students can assemble, test, and operate various classes of small satellites in a valuable learning experience that will prepare them to work on larger projects. The true benefit of a PocketQube, CanSat, or femtosatellite is that they can be built with less money, less experience, and less long-term commitment while providing a scaled version of the real-life satellite development process that will engage and inspire students. …

Novel Gain-Of-Function Mutations In The Telomerase Rna Of Saccharomyces Cerevisiae, Keenan Conley, Madison Frazier, Melissa Mefford

2022 Celebration of Student Scholarship - Poster Presentations

Yeast are eukaryotic organisms that contain liner chromosomes. At the ends of these chromosomes, there are special regions known as telomeres. Telomeres are composed of non-coding nucleotides that serve to protect the vital coding DNA found throughout the rest of the chromosome. During the process of DNA replication, however, telomeres become progressively shorter which can lead to cellular senescence. To solve this issue, yeast express the enzyme telomerase. Telomerase is composed of a telomerase reverse transcriptase protein (TERT) and telomerase RNA (TLC1) in yeast. When telomeres become too short, telomerase extends telomeres by adding to the 3’ overhang. Telomeres shorten …

Technology In Workplace Communication By Generation, Hannah Meadows, Christy Trent

2022 Celebration of Student Scholarship - Poster Presentations

The purpose of this research study was to determine the generational differences in the use of communication technology in the workplace. Most employees feel a deeper sense of connection to those in their own generational group. This survey looks at ideologies across four generations, Baby Boomers, Generation X, Millennials, and Generation Z, to help give employers some insight into why employees feel this way and encourage better communication in the workforce.

Fungi In A Warmer World: Fungal Diversity In The Tropical Miocene Climate Optimum Of The Clarkia Region Of Idaho, Usa, J. Fairchild, L. Lennex-Stone, T. Horsfall, L. Tarlton, S. Jones, A. Caldwell, O. Vanderespt, L. Smallwood, A. Patel, J. O'Keefe, I. Romero, N. Nunnez Otano, M. Pound

2022 Celebration of Student Scholarship - Poster Presentations

A knowledge gap associated with how fungal communities change in response to climate was identified in the 2018 State of the World Fungi report (Willis, 2018). While it is virtually impossible to test in the present, fungal assemblage changes can be studied in sediments from a warmer-than-present period such as the Miocene Climate Optimum (MCO) (Romero et al. 2021; O’Keefe 2017). The Fungi in a Warmer World project aims to generate and analyze a global-scale data set of fungal biodiversity, ecology, and associated flora from MCO sediments. This dataset will be used to model past fungal assemblage changes across the …

Morphometrics Of The Rare Earwigfly Merope Tuber, Nicholas Finch, Sean O'Keefe

2022 Celebration of Student Scholarship - Poster Presentations

Merope tuber Newman (Mecoptera: Meropeidae) is a rare North American species of earwigfly, which is closely related to common scorpionflies. “Earwigfly” refers to three different species: the Western Australian Austromerope poulton, the South American Austromerope brasiliensis, and finally, the Eastern North American Merope tuber. This last species was the focus of our study. Johnson’s 1995 study was based on 160 earwigflies, where he measured the female abdomen length, male and female forewing length, and male basistylus and dististylus length. In their 2014 study, based on 82 earwigflies, Skvarla, Hartshorn, and Dowling measured head width, pronotum width, forewing length, abdomen length, …

Encouraging Increased Ppe Compliance: A Quality Improvement Project, Christen Ballard, Hannah Carpenter, Skyler Harper, Angela Marcum, Suzi White

2022 Celebration of Student Scholarship - Poster Presentations

Personal protective equipment (PPE) is used to minimize infection transmission from patient to healthcare worker, or vice versa. During a clinical rotation in a large urban teaching hospital, it was noted that a number of nurses failed to adhere to proper PPE policies. A literature review focusing on thirty-two studies found that average nurse compliance to proper PPE is 34%. This review included an overall discussion of factors affecting compliance, as well as a more in-depth review of the effect of education on PPE compliance, how comfort and proper fitting affects compliance, and the general effectiveness of PPE when used …

“The Never Ending Story:” Circularizing Ch. Viii In Saccharomyces Cerevisiae, Chisom Iloegbunam, Melissa Mefford

2022 Celebration of Student Scholarship - Poster Presentations

Chromosomes are molecules of DNA that hold large quantities of genetic material in an organism. Generally, in nature, eukaryotic chromosomes are linear while prokaryotic chromosomes are circular. Since they are linear, eukaryotic chromosomes possess telomeres, or repetitive nucleotide sequences, on their ends. This significant difference in structure requires different additions to the basic replication process. In prokaryotes, replication starts at a single point on the chromosome and continues until the whole chromosome has been copied. In eukaryotes, replication happens in fragments with multiple starting points. Because of this more complicated replication process, the free 3’ ends of our DNA cannot …

College Students’ Knowledge And Use Of Herbal Supplements: Informed Decision, Health Risk, Or Marketing Ploy?, Jillian Wallshield, Wilson Gonzalez-Espada

2022 Celebration of Student Scholarship - Poster Presentations

The purpose of this study is to better understand the extent to which college students from Eastern Kentucky use Herbal Supplements (HS). The research questions for this study were:

• How do the participants find out about HS?

• How helpful do participants perceive the HS they take to be?

• What misconceptions do participants have regarding HS?

• Does a positive or negative attitude towards science change the usage rate of HS?

A New Colorimetric Assay Of Apurinic/Apyrimidinic (Abasic) Sites Of Deoxyribonucleic Acid (Dna) Using Bicinchoninic Acid [, Isaiah O. Stephens, Elizabeth M. Thomas

2022 Celebration of Student Scholarship - Poster Presentations

Apurinic/apyrimidinic (AP or abasic) sites are DNA lesions that result from the loss of a nucleobase by hydrolysis of the N-glycosyl bond. It is estimated that AP sites are the most frequent lesions in cells with about 10,000-50,000 times per day per cell under typical aerobic conditions [2]. The formation of AP sites is caused by environmental and cancer therapeutic genotoxins such as alkylating agents, oxidizing agents, ionizing radiation, and ultraviolet radiation.