Life Sciences Commons^™

Als-Associated Kif5a Mutations Abolish Autoinhibition Resulting In A Toxic Gain Of Function, Desiree M. Barron, Adam R. Fenton, Sara Saez-Atienzar, Anthony Giampetruzzi, Aparna Sreeram, Shankaracharya, Pamela J. Keagle, Victoria R. Doocy, Nathan J. Smith, Eric W. Danielson, Megan Andresano, Mary C. Mccormack, Jaqueline Garcia, Valérie Bercier, Ludo Van Den Bosch, Jonathan R. Brent, Claudia Fallini, Brian J. Traynor, Erica L.F. Holzbaur, John E. Landers

Biochemistry -- Faculty Publications

Understandingthepathogenicmechanismsof diseasemutations is critical toadvancingtreatments.ALS-associated mutations in the gene encoding the microtubulemotor KIF5A result in skipping of exon 27 (KIF5ADExon27) and the encoding of a protein with a novel 39 amino acid residue C-terminal sequence. Here, we report that expression of ALS-linked mutant KIF5A results in dysregulated motor activity, cellular mislocalization, altered axonal transport, and decreased neuronal survival. Single-molecule analysis revealed that the altered C terminus of mutant KIF5A results in a constitutively active state. Furthermore,mutant KIF5A possesses altered protein and RNA interactions and its expression results in altered gene expression/splicing. Taken together, our data support the hypothesis that causative …

Harmonizing Model Organism Data In The Alliance Of Genome Resources., Alliance Of Genome Resources Consortium, Anna V. Anagnostopoulos, Susan M. Bello, Judith A. Blake, Olin Blodgett, Carol J. Bult, Karen R. Christie, Mary E. Dolan, Paul Hale, James A. Kadin, Monica S. Mcandrews, Howie Motenko, David R. Shaw, Constance M. Smith, Cynthia L. Smith, Monika Tomczuk, Laurens G. Wilming

Faculty Research 2022

The Alliance of Genome Resources (the Alliance) is a combined effort of 7 knowledgebase projects: Saccharomyces Genome Database, WormBase, FlyBase, Mouse Genome Database, the Zebrafish Information Network, Rat Genome Database, and the Gene Ontology Resource. The Alliance seeks to provide several benefits: better service to the various communities served by these projects; a harmonized view of data for all biomedical researchers, bioinformaticians, clinicians, and students; and a more sustainable infrastructure. The Alliance has harmonized cross-organism data to provide useful comparative views of gene function, gene expression, and human disease relevance. The basis of the comparative views is shared calls of …

Alewife (Alosa Pseudoharengus) Contributions To Lake Phosphorus Budgets: Implications For Management, Meg "Rue" Farley Thurrell Ms

Student Scholarship

As human-made dams are removed and fish passage at dams is improved in

support of restoration efforts, anadromous alewives (Alosa pseudoharengus) gain

access to historic spawning grounds. As they migrate to spawn, adult alewives import

marine derived nutrients to lakes and impoundments. Young of year alewives (juveniles)

export nutrients as they emigrate to the sea. These nutrients support primary

production and are incorporated into freshwater food webs. However, many lakes in

New England are mesotrophic or eutrophic. Additional nutrients, specifically

phosphorus (P), may exacerbate water quality issues. To examine these issues, I

compared outputs ofan alewife nutrient and population model …

Faster Intercalation Pseudocapacitance Enabled By Adjustable Amorphous Titania Where Tunable Isomorphic Architectures Reveal Accelerated Lithium Diffusivity, Wessel Van Den Bergh, Taylor Larison, Maximilliano Jesus Jara Fornerod, Prof. Stefan Guldin, Morgan Stefik

Faculty Publications

Intercalation pseudocapacitance is a faradaic electrochemical phenomenon with high power and energy densities, combining the attractive features of capacitors and batteries, respectively. Intercalation pseudocapacitive responses exhibit surface-limited kinetics by definition, without restriction from the collective of diffusion-based processes. The surface-limited threshold (SLT) corresponds to the maximum voltage sweep rate (vSLT) exhibiting a predominantly surface-limited current response prior to the onset of diffusion-limitations. Prior studies showed increased lithium diffusivity for amorphous titania compared to anatase. Going beyond prior binary comparisons, here a continuum of amorphous titania configurations were prepared using a series of calcination temperatures to tailor both amorphous …

Environment And Co-Occurring Native Mussel Species, But Not Host Genetics, Impact The Microbiome Of A Freshwater Invasive Species (Corbicula Fluminea), Marlène Chiarello, Jamie R. Bucholz, Mark Mccauley, Stephanie N. Vaughn, Garrett W. Hopper, Irene Sánchez González, Carla L. Atkinson, Jeffrey D. Lozier, Colin R. Jackson

Faculty and Student Publications

The Asian clam Corbicula fluminea (Family: Cyneridae) has aggressively invaded freshwater habitats worldwide, resulting in dramatic ecological changes and declines of native bivalves such as freshwater mussels (Family: Unionidae), one of the most imperiled faunal groups. Despite increases in our knowledge of invasive C. fluminea biology, little is known of how intrinsic and extrinsic factors, including co-occurring native species, influence its microbiome. We investigated the gut bacterial microbiome across genetically differentiated populations of C. fluminea in the Tennessee and Mobile River Basins in the Southeastern United States and compared them to those of six co-occurring species of native freshwater mussels. …

Development And Validation Of An Edna Protocol For Monitoring Endemic Asian Spiny Frogs In The Himalayan Region Of Pakistan, Muhammad Saeed, Muhammad Rais, Ayesha Akram, Maggie R. Williams, Kenneth F. Kellner, Syed A. Hashsham, Drew R. Davis

School of Earth, Environmental, & Marine Sciences Faculty Publications

Wildlife monitoring programs are instrumental for the assessment of species, habitat status, and for the management of factors affecting them. This is particularly important for species found in freshwater ecosystems, such as amphibians, as they have higher estimated extinction rates than terrestrial species. We developed and validated two species-specific environmental DNA (eDNA) protocols and applied them in the field to detect the Hazara Torrent Frog (Allopaa hazarensis) and Murree Hills Frog (Nanorana vicina). Additionally, we compared eDNA surveys with visual encounter surveys and estimated site occupancy. eDNA surveys resulted in higher occurrence probabilities for both A. …

Physiological And Biochemical Thermal Stress Conditions Of The Ribbed Mussel, Geukensia Demissa, From Exposed And Less Exposed Areas In The Intertidal Salt Marsh On Tybee Island, Georgia., Jody E. Erber

Honors College Theses

Geukensia demissa, the ribbed mussel, is a keystone species of Georgia’s coastline that is at risk of experiencing detrimental thermal stress due to climate change. G. demissa interacts positively with a species of salt marsh cordgrass, Spartina alterniflora. Mussels form aggregates beneath salt marsh cordgrass stems where they are shaded and less exposed to sun rays. However, some mussels end up in areas which lack cordgrass and are directly exposed to sun rays. Body temperatures of mussels from exposed areas were found to be higher than mussels from less exposed areas. Thermal stress levels of mussels can be …

Variation In Bacterial Community Structure Under Long-Term Fertilization, Tillage, And Cover Cropping In Continuous Cotton Production, Ning Duan, Lidong Li, Xiaolong Liang, Aubrey Fine, Jie Zhuang, Mark Radosevich, Sean M. Schaeffer

Department of Agronomy and Horticulture: Faculty Publications

Agricultural practices alter the structure and functions of soil microbial community. However, few studies have documented the alterations of bacterial communities in soils under long-term conservation management practices for continuous crop production. In this study, we evaluated soil bacterial diversity using 16S rRNA gene sequencing and soil physical and chemical properties within 12 combinations of inorganic N fertilization, cover cropping, and tillage throughout a cotton production cycle. Soil was collected from field plots of the West Tennessee Agriculture Research and Education Center in Jackson, TN, United States. The site has been under continuous cotton production for 38 years. A total …

Comparison Of Flavonoid Intake Assessment Methods Using Usda And Phenol Explorer Databases: Subcohort Diet, Cancer And Health-Next Generations—Max Study, Fabian Lanuza, Nicola P. Bondonno, Raul Zamora-Ros, Agnetha Linn Rostgaard-Hansen, Anne Tjønneland, Rikard Landberg, Jytte Halkjær, Cristina Andres-Lacueva

Research outputs 2022 to 2026

Flavonoids are bioactive plant compounds that are widely present in the human diet. Estimating flavonoid intake with a high degree of certainty is challenging due to the inherent limitations of dietary questionnaires and food composition databases. This study aimed to evaluate the degree of reliability among flavonoid intakes estimated using four different approaches based on the two most comprehensive flavonoid databases, namely, United States Department of Agriculture (USDA) and Phenol Explorer (PE). In 678 individuals from the MAX study, a subcohort of the Diet, Cancer and Health-Next Generations cohort, dietary data were collected using three 24-h diet recalls over 1 …

Impact Of Hydrologic Alteration On Brazos River Pelagophilic Minnows, Christopher M. Taylor, Kevin B. Mayes

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

The lower Brazos River has been profoundly altered since the construction of multiple impoundments. The resulting river regulation poses problems for organisms adapted to a natural flow regime and is thought to play a major role in the imperilment of seven native minnows that synchronize spawning during high flow pulses. Four of the species are presumably extirpated from the region, but their declines and losses differed across time. To address potential reasons for species’ declines, we assessed pre- and post-impoundment changes in flow regime from five gaging stations across the lower Brazos River to identify river flow characteristics that depart …

Correction To The Probability Of Spotted Lanternfly, Lycorma Delicatula (Hemiptera: Fulgoridae), Escape Differs Among Life Stages And Between Two Trapping Techniques Commonly Used By Landowners, Sticky Bands And Duct Tape. The Great Lakes Entomologist 53(3-4): 170-177., Matthew Desko, Carolyne Schiebel, Samantha Silverman, Jessica Bickel, Karen Felton, Jennifer L. Chandler

The Great Lakes Entomologist

This is a manuscript correction in reference to: Desko M, Schiebel C, Silverman S, Bickel J, Felton K, and Chandler JL. (2020) The Probability of spotted lanternfly, Lycorma delicatula (Hemiptera: Fulgoridae), escape differs among life stages and between two trapping techniques commonly used by landowners, sticky bands and duct tape. The Great Lakes Entomologist 53(3-4): 170-177.

First Records Of Stelis Permaculata Cockerell (Hymenoptera: Megachilidae) In Minnesota, United States Of America And Manitoba, Canada, Colleen D. Satyshur, Thea A. Evans, Britt M. Forsberg, Jason Gibbs

The Great Lakes Entomologist

Abstract

New records are reported for the cleptoparasitic bee Stelis permaculata Cockerell (Hymenoptera: Megachilidae) from Minnesota, United States of America and Manitoba, Canada. Minnesota records come from trap-nests, which also collected the host, Heriades carinata Cresson.

Life History And Laboratory Rearing Of The Red Admiral, Vanessa Atalanta (Lepidoptera: Nymphalidae), Layne Leake, Kevin B. Rice

The Great Lakes Entomologist

The red admiral butterfly, Vanessa atalanta (Linneaus, 1758) (Lepidoptera: Nymphalidae) is a globally distributed species and model organism for studying migration patterns and effects of climate change. Most previous red admiral research focused on wild populations. Establishing laboratory colonies allow for experimentation with a multitude of lab-based plant-insect interactions. We describe red admiral butterfly life history and laboratory rearing methods.

Rediscovery Of Gnoriste Macra Johannsen In Wisconsin (Diptera: Mycetophilidae: Gnoristinae), Daniel K. Young

The Great Lakes Entomologist

Gnoriste macra Johannsen was described from Whitefish Bay, Wisconsin in 1912. The type locality is now a highly residential region a few miles north of the center of Milwaukee. Herein, collection events are reported for G. macra from Sauk County in southcentral Wisconsin. These new records, nearly a century after the only other literature record, indicate the species still thrives in Wisconsin though likely extirpated from the type locality. The specimens were recovered from unbaited Townes Malaise traps during mid- to late May, 2006 and 2020. Cranial anatomy and functional morphology of the head suggests G. macra may be a …

New Records Of Aphids (Hemiptera: Aphididae) On Industrial Hemp In The Us Midwest, Doris M. Lagos-Kutz, Christina D. Difonzo, Glen L. Hartman

The Great Lakes Entomologist

Industrial hemp (Cannabis sativa L.) production in the USA is increasing, and with it the list of insects colonizing the crop. In this article, we report new records of Aphis craccivora Koch 1854, Aphis fabae Scopoli, Aphis gossypiiGlover 1763, Aphis spiraecola Patch 1914 and Myzus persicae (Sulzer 1776) on industrial hemp in East Lansing, Michigan in fall 2020. In addition, between 2017 and 2020, the number of suction trap sites detecting P. cannabisincreased, and as well as the number of sites with multiple weeks of detections. The timing of detection changed, from only late season (fall migrants) …

Annotated Checklist Of The Notonectidae (Hemiptera: Heteroptera) Or "Backswimmers" Of Iowa, Edwin L. Freese, Stephen W. Chordas

The Great Lakes Entomologist

A review of the Notonectidae or backswimmers of Iowa is presented. New state records for Buenoa macrotibialis Hungerford, Buenoa scimitra Bare, Notonecta insulata Kirby, Notonecta irrorata Uhler, and Notonecta lunata Hungerford are included.

A Survey Of The Beetles (Insecta: Coleoptera) In Several Natural Areas Across Indiana, U.S.A., Kyle E. Schnepp, R. Michael Brattain, Gareth S. Powell

The Great Lakes Entomologist

A broad survey of the beetle (Insecta: Coleoptera) fauna of several managed parks and natural areas was undertaken over the past decade across the state of Indiana, USA. These areas represent many of the habitat types present in Indiana including forests, grassland, freshwater streams, and wetlands, as well as more unique habitats found in limited areas in the state such as sand dunes and cypress swamps. The specific localities are: Indiana Dunes State Park, Kankakee Sands, Ross Hills Park, Shades State Park, Muscatatuck National Wildlife Refuge, and Twin Swamps Nature Preserve. Beetles were surveyed using a variety of active and …

New State And Provincial Records For Some Nearctic True Bugs (Hemiptera: Heteroptera) From The Illinois Natural History Survey Insect Collection, Daniel R. Swanson

The Great Lakes Entomologist

157 state records for 133 heteropteran species in 99 genera in 33 different families from 40 states are newly-reported based on material housed in the Illinois Natural History Survey Insect Collection. Families represented by new records include Acanthosomatidae, Alydidae, Anthocoridae, Aradidae, Blissidae, Belostomatidae, Coreidae, Corixidae, Cymidae, Gelastocoridae, Geocoridae, Hebridae, Hydrometridae, Largidae, Lasiochilidae, Lygaeidae, Mesoveliidae, Miridae, Nabidae, Naucoridae, Nepidae, Notonectidae, Piesmatidae, Pleidae, Reduviidae, Rhopalidae, Rhyparochromidae, Saldidae, Schizopteridae, Scutelleridae, Thyreocoridae, Tingidae, and Veliidae, and the following 9 records represent new family records: Acanthosomatidae (West Virginia), Cymidae (Kentucky), Hebridae (Tennessee), Lygaeidae (Delaware), Naucoridae (Georgia), Piesmatidae (Mississippi, North Dakota), Pleidae (North Dakota), and Schizopteridae …

An Updated Checklist To The Non-Biting Midges (Chironomidae) Of Illinois, Daniel R. Swanson, Erika E. Bilger

The Great Lakes Entomologist

The Chironomidae, or non-biting midges, of Illinois have been untreated since the early 20^th century, and the high level of taxonomic flux in the family necessitates an update, particularly for a group with importance as bio-indicators of freshwater ecosystem health. Herein, 181 species of Chironomidae in 76 genera are recorded from the state of Illinois. Each species entry is annotated with the first Illinoisan record of the species. Two genera, Brillia Kieffer, 1913 and Prodiamesa Kieffer, 1906, are recorded for the first time from Illinois, as are three species: Xenochironomus xenolabis (Kieffer in Thienemann and Kieffer, 1916) (Chironominae); Brillia …

Management Of Wireworms In Sweet Potatoes With Persistent Ny Entomopathogenic Nematodes, Elson Shields, Antonio Testa, Teresa Rusinek, Charles Bornt

The Great Lakes Entomologist

Wireworms are the larval stages of click beetles (Coleoptera: Elateridae) and common polyphagous soil dwelling pests feeding on different plant parts including seeds, roots, stems, and tubers inhibiting plant growth eventually leading to plant death. With the ban of persistent synthetic insecticides such as lindane in 2009 due to negative effects on the environment, no effective control tactics (chemical or biological) are available for wireworms. Some entomopathogenic nematode species/strains have been reported to attack wireworms in the soil, causing death. Non-native EPN species have the advantage of being easily available commercial products for insect pest control. However, these strains do …

Cover Art For Tgle Vol. 54 Nos. 3 & 4

The Great Lakes Entomologist

Cover Art for TGLE Vol. 54 Nos. 3 & 4

Cover Pages For Tgle Vol. 54 Nos. 3 & 4

The Great Lakes Entomologist

Cover Pages for TGLE Vol. 54 Nos. 3 & 4

Full Issue For Tgle Vol. 54 Nos. 3 & 4

The Great Lakes Entomologist

Full issue for TGLE Vol. 54 Nos. 3 & 4

Smokejumper Obituary: Sleight, James Scott “Jim” (Mccall 1977), National Smokejumper Association

Smokejumper Obituaries

No abstract provided.

Optimization Of Sample Unit Size For Sampling Stink Bugs (Hemiptera: Pentatomidae) In Soybean, Arthur V. Ribeiro, Rafael Carlesso Aita, Daniela T. Pezzini, Christina D. Difonzo, Thomas Hunt, Janrt J. Knodel, Christian H. Krupke, Lia Marchi-Werle, Andrew P. Michel, Nicholas J. Seiter, Robert J. Wright, William D. Hutchison, Robert L. Koch

Department of Entomology: Faculty Publications

Cost-effective and reliable sampling procedures are crucial for integrated pest management. Sweep net sampling is commonly used for stink bugs (Hemiptera: Pentatomidae) in soybean, with sample size being the number of sets of sweeps, and sample unit size the number of sweeps in each set. Sample unit size has received little attention, but can affect sampling parameters. Here, two sample unit sizes (10 vs. 25 sweeps) were compared for the sampling of stink bug taxa. On average, sampling for stink bugs took 3.6 more minutes with the 25-sweep than with the 10-sweep sample unit size. Generally, estimates of the mean …

Targeted Osmotic Lysis: A Novel Approach To Targeted Cancer Therapies, Harry J. Gould, Dennis Paul

School of Medicine Faculty Publications

The conventional treatment of cancer has been based on the delivery of non-selective toxins and/or ionizing energy that affect both the cancer and normal tissues in the hope of destroying the offending disease before killing the patient. Unfortunately, resistance often develops to these treatments and patients experience severe, dose-limiting adverse effects that reduce treatment efficacy and compromise quality of life. Recent advances in our knowledge of the biology of tumor cells and their microenvironment, the recognition of surface proteins that are unique to specific cancers and essential to cell growth and survival and signaling pathways associate with invasion and metastasis …

Host-Pathogen Coevolution Between Tasmanian Devils (Sarcophilus Harrisii) And Devil Facial Tumor Disease, Dylan Garret Gallinson

USF Tampa Graduate Theses and Dissertations

Coevolution is a driving force of rapid evolution, yet the complexity of coevolutionary interactions has made it difficult to characterize the genomic basis of traits mediating such relationships. Coevolutionary dynamics are especially important in host-pathogen systems where the host and pathogen must constantly adapt to one another. The Tasmanian devil and its species-specific transmissible cancer, devil facial tumor disease (DFTD), provide the rare opportunity to study host-pathogen coevolution in a complex natural system. Extensive spatiotemporal devil sampling, high linkage disequilibrium in devils, and a large selective pressure imposed by DFTD facilitate a system tractable for study. Here, we characterized devil …

Ubjective Information And Survival In A Simulated Biological System, Tyler S. Barker, Massimiliano Pierobon, Peter J. Thomas

School of Computing: Faculty Publications

Information transmission and storage have gained traction as unifying concepts to characterize biological systems and their chances of survival and evolution at multiple scales. Despite the potential for an information-based mathematical framework to offer new insights into life processes and ways to interact with and control them, the main legacy is that of Shannon’s, where a purely syntactic characterization of information scores systems on the basis of their maximum information efficiency. The latter metrics seem not entirely suitable for biological systems, where transmission and storage of different pieces of information (carrying different semantics) can result in different chances of survival. …

Privacy-Preserving Logistic Regression With Secret Sharing, Ali Reza Ghavamipour, Fatih Turkmen, Xiaoqian Jiang

Faculty, Staff and Student Publications

BACKGROUND: Logistic regression (LR) is a widely used classification method for modeling binary outcomes in many medical data classification tasks. Researchers that collect and combine datasets from various data custodians and jurisdictions can greatly benefit from the increased statistical power to support their analysis goals. However, combining data from different sources creates serious privacy concerns that need to be addressed.

METHODS: In this paper, we propose two privacy-preserving protocols for performing logistic regression with the Newton-Raphson method in the estimation of parameters. Our proposals are based on secure Multi-Party Computation (MPC) and tailored to the honest majority and dishonest majority …

Fishing Without A Scale, Kaleb Miller, Luke Lemaitre

Student Scholarship - College of Business

Our study looks at different species of fish and uses their cross-length and volume to find correlations in weight among the different fish. The different type of fish that we compare include perch, pike, parkki, whitefish, bream, roach, and smelt. The dependent variable that we are using is weight. Once we predict the weight of the individual fish, we will also compare that weight to other fish species. The result of the data are that there is linearity, where the adjusted R squared is between 0.94 and 0.97, depending on our model. Therefore, 94 to 97 percent of the variability …