Life Sciences Commons^™

The Impact Of Pediatric Opioid-Related Visits On Us Emergency Departments, Tiffany Champagne-Langabeer, Marylou Cardenas-Turanzas, Irma T Ugalde, Christine Bakos-Block, Angela L Stotts, Lisa Cleveland, Steven Shoptaw, James R Langabeer

Faculty, Staff and Student Publications

BACKGROUND: While there is significant research exploring adults' use of opioids, there has been minimal focus on the opioid impact within emergency departments for the pediatric population.

METHODS: We examined data from the Agency for Healthcare Research, the National Emergency Department Sample (NEDS), and death data from the Centers for Disease Control and Prevention. Sociodemographic and financial variables were analyzed for encounters during 2014-2017 for patients under age 18, matching diagnoses codes for opioid-related overdose or opioid use disorder.

RESULTS: During this period, 59,658 children presented to an ED for any diagnoses involving opioids. The majority (68.5%) of visits were …

Volume 8 Full Text, Bjur Staff

Butler Journal of Undergraduate Research

No abstract provided.

Table Of Contents And Front Matter, Bjur Staff

Butler Journal of Undergraduate Research

No abstract provided.

Comparison Of Training Load Between Ncaa Diii Tennis Resistance Training, Practices, And Competitions, Lauren Pascadlo

Student Scholar Showcase

Background: There are 7553 NCAA Division III tennis players, who play with less training coordination than elite players. Division III tennis load research is highly underdeveloped with most tennis research focusing on elite players. Additionally, practice training loads must be similar to match training loads so athletes are physically and mentally prepared for competition.

Purpose: To describe the training load in Division III tennis players during strength and conditioning, practices, and competition.

Methods: 7 Male and female division II tennis players attended 2 regularly scheduled practice, competition, and resistance training sessions. Training load was measured as external (GPS data consisting …

The Effect Of Ethanolic Echinacea Purpurea Root Extracts On The Production Of Pro-Inflammatory Mediators In Raw 264.7 Murine Macrophages, Trey T. Nguyen

Student Scholar Showcase

Echinacea purpurea, commonly known as the purple coneflower, is utilized in medicinal preparations due to potential immunostimulatory and anti-inflammatory properties. Present bioactive metabolites such as alkamides, polysaccharides, and caffeic acid derivatives provide pharmacological elements essential to mitigating symptoms produced by upper respiratory illnesses. The chemical composition of E. purpurea holds medicinal value, but there remains controversy surrounding the efficacy of its use in medicine. The purpose of this study is to examine the effects of an 75% ethanolic E. purpurea root extract on LPS (lipopolysaccharide)-stimulated RAW 264.7 murine macrophages. Treatment groups included ethanolic E. purpurea extract (3µL, 15µL, 30µL), …

Carbonyl Iron Vs Ferrous Sulfate On Blood Iron Markers In Male Division Iii Cross-Country Athletes, Sam Gunter

Student Scholar Showcase

Competitive distance runners are often predisposed to developing iron deficiency. This study aimed to determine if carbonyl iron was more effective at maintaining blood iron markers and minimizing overall fatigue and GI stress than ferrous sulfate. In this randomized, independent groups study, 7 male, division III cross-country athletes were supplemented with either carbonyl iron or ferrous sulfate for 6 weeks. Blood hemoglobin, hematocrit, subjective GI distress, subjective fatigue, and relative exertion of recent training were assessed at baseline, 3 and 6 weeks. Results will be analyzed via a detailed analysis of variance to determine significant differences in outcome measures. Statistical …

The Adoption Of Conservation Practices And Program Participation Among Socially Disadvantaged Agricultural Producers: A Meta-Analysis, Myles A. Brown

LSU Master's Theses

As climate change has become undeniable in recent years, it has become increasingly important for the agriculture industry to address conservation. Within the agriculture industry, small farmers are usually the ones who take on this burden on a daily basis. However, some socially disadvantaged agricultural producers face unique challenges compared to the average farmer, which may impede their ability to adopt the necessary conservation practices or participate in conservation programs. This review sought to provide a definitive economic analysis on the possible effect of race on conservation adoption and program participation, as there is not much research on this topic. …

The Plant And The Pathogen: Elucidating The Relationship Between Root-Knot Nematodes, Ph Levels, And Arabidopsis Thaliana Development, Rachel Garnett

Student Scholar Showcase

Root-knot nematodes (RKNs) within the Meloidogyne genus are considered one of the largest threats to plant health and subsequent crop yield and profit (Forghani and Hajihassani 2020; Bernard et al. 2017). As a pest that presents global consequences, its mitigation through sustainable interventions may confer results for the treatment of similar plant pathogens. A member of the Brassicaeae family, Arabidopsis thaliana, was chosen for this study to determine plant responses to RKN-standard pH media. pH was varied between control and experimental groups, and phenotypic variables of primary root length and plant height were observed and analyzed. Wild-type Arabidopsis thaliana …

Investigating Alternative Induction Of The S. Aureus 80a Prophage, Jody Caretti

Student Scholar Showcase

Widespread antibiotic resistance has quickly become one of the most concerning crises affecting modern medicine, especially in cases of Staphylococcal infections, which have become resistant to all ��-lactam antibiotics. As a result, research into alternative forms of treatment for bacterial infections is a top priority. Bacteriophage therapy is a popular candidate for replacing antibiotics, due to the way the viruses interact with the bacteria. The 80�� prophage integrates its DNA into Staphylococcus aureus strain 10616 via the lysogenic cycle, and replicates with the bacterial cell. When stressed, the bacteria stop replicating and the prophage stimulates the lytic cycle, activating transcription …

Ecosystem Service Values Of Gardens In The Yellow River Basin, China, Ge Qianqian, Xu Wenjie, Fu Meichen, Han Yingxin, An Guoqiang, Xu Yuetong

Journal of Arid Land

Studies on the ecosystem service value (ESV) of gardens are critical for informing evidence- based land management practices based on an understanding of the local ecosystem. By analyzing equivalent value factors (EVFs), this paper evaluated the values of 11 ecosystem services of gardens in the Yellow River Basin of China in 2019. High-precision land use survey data were used to improve the accuracy of the land use classification, garden areas, and spatial distribution of the ESVs of gardens. The results showed that garden ecosystem generally had high ESVs, especially in terms of the ESV of food production, which is worthy …

Neural Substrates Of Homing Pigeon Spatial Navigation: Results From Electrophysiology Studies, Gerald E. Hough

College of Science & Mathematics Departmental Research

Over many centuries, the homing pigeon has been selectively bred for returning home from a distant location. As a result of this strong selective pressure, homing pigeons have developed an excellent spatial navigation system. This system passes through the hippocampal formation (HF), which shares many striking similarities to the mammalian hippocampus; there are a host of shared neuropeptides, interconnections, and its role in the storage and manipulation of spatial maps. There are some notable differences as well: there are unique connectivity patterns and spatial encoding strategies. This review summarizes the comparisons between the avian and mammalian hippocampal systems, and the …

Crispr-Mediated Multiplexed Live Cell Imaging Of Nonrepetitive Genomic Loci With One Guide Rna Per Locus., Patricia A Clow, Menghan Du, Nathaniel L. Jillette, Aziz Taghbalout, Jacqueline J Zhu, Albert Cheng

Faculty Research 2022

Three-dimensional (3D) structures of the genome are dynamic, heterogeneous and functionally important. Live cell imaging has become the leading method for chromatin dynamics tracking. However, existing CRISPR- and TALE-based genomic labeling techniques have been hampered by laborious protocols and are ineffective in labeling non-repetitive sequences. Here, we report a versatile CRISPR/Casilio-based imaging method that allows for a nonrepetitive genomic locus to be labeled using one guide RNA. We construct Casilio dual-color probes to visualize the dynamic interactions of DNA elements in single live cells in the presence or absence of the cohesin subunit RAD21. Using a three-color palette, we track …

Feasibility Of Tubulin As A Control For Gene Expression Following Transfection In Mouse Monocyte/Macrophage-Like Cells, Ankita Chabra

Honors Program Theses and Research Projects

Transfection, which is the ability to modify host cells’ genetic content, has broad application in studying normal cellular processes, molecular mechanism of disease and gene therapy. There are several transfection techniques, and all require either a control or a reference gene. Commonly used controls for transfection experiments are housekeeping genes, which maintain expression for a given cell/tissue, experimental conditions, and treatment. However, recent research has uncovered that expression levels of housekeeping genes may vary depending on the gene, cell type and experimental conditions. A growing body of evidence demonstrates that housekeeping genes are inadequate internal standards for measuring gene expression …

Farm Succession Planning: Why And How?, Allan Vyhnalek

Cornhusker Economics

If you visit with an ag professional and ask them “What percentage of farmers and ranchers have an estate plan, or an up-to-date estate/succession plan?” the answer will be half, or just less than half. This article will examine why that happens and give some simple steps for anyone to follow to create an up-to-date estate or succession plan. Farmers and ranchers don’t have a plan for a variety of reasons. These might include not ever planning to retire, not wanting to give up control, or not wanting to admit that they will ever pass away. Too often, we don’t …

Nanoparticle Quantification And Distribution On Fluorescently Coated Angioplasty Balloons, Allison Dobson Zieschang

Honors Capstones

No abstract provided.

Lis1 And Ndel1 Regulate Axonal Trafficking Of Mitochondria In Mature Neurons, Jai P. Pandey, Liang Shi, Remi A. Brebion, Deanna S. Smith

Faculty Publications

Defective mitochondrial dynamics in axons have been linked to both developmental and late-onset neurological disorders. Axonal trafficking is in large part governed by the microtubule motors kinesin-1 and cytoplasmic dynein 1 (dynein). Dynein is the primary retrograde transport motor in axons, and mutations in dynein and many of its regulators also cause neurological diseases. Depletion of LIS1, famous for linking dynein deregulation to lissencephaly (smooth brain), in adult mice leads to severe neurological phenotypes, demonstrating post-developmental roles. LIS1 stimulates retrograde transport of acidic organelles in cultured adult rat dorsal root ganglion (DRG) axons but findings on its role in mitochondrial …

The Effects Of A Periodized Strength And Conditioning Program On Overhand Throwing Velocity, Underhand Throwing Velocity And Off The Bat Velocity In Diii Collegiate Athletes, Sage Jenkins

Undergraduate Distinction Papers

Overhand throwing velocity, underhand throwing velocity and off the bat velocity are measures that impact performance of softball athletes. The purpose of strength and conditioning programs is to improve athletic performance for athletes. Strength and conditioning coaches can achieve this through programming. One aspect of programming that this study looked at is periodization. The purpose of this study was to examine the effects of a periodized strength and conditioning program on these performance measurements in NCAA DIII collegiate softball athletes. Sixteen (n=16) NCAA DIII softball athletes were selected based on their participation on the Otterbein softball team. The athletes underwent …

Flooding Tolerance Of Four Tropical Peatland Tree Species In A Nursery Trial, Hesti L. Tata, Hani S. Nuroniah, Diandra A. Ahsania, Haning Anggunira, Siti N. Hidayati, Meydina Pratama, Istomo Istomo, Rodney Chimner, Meine Van Noordwijk, Randall Kolka

Michigan Tech Publications, Part 1

In order to facilitate hydrological restoration, initiatives have been conducted to promote tree growth in degraded and rewetted peatlands in Indonesia. For these initiatives to be successful, tree seedlings need to be able to survive flooding episodes, with or without shade. We investigated the survival rates and the formation of adventitious roots in the case of four tree species exposed to combinations of different shading and water levels under controlled conditions in a nursery, with artificial rainwater and with peat soil as the medium. The research focused on the following questions (i) whether trees can grow on flooded peat soils; …

Early Pleistocene Large Mammals From Maka’Amitalu, Hadar, Lower Awash Valley, Ethiopia, John Rowan, Ignacio A. Lazagabaster, Christopher J. Campisano, Faysal Bibi, René Bobe, Jean-Renaud Boisserie, Stephen R. Frost, Tomas Getachew, Christopher C. Gilbert, Margaret E. Lewis, Sahleselasie Melaku, Eric Scott, Antoine Souron, Lars Werdelin, William H. Kimbel, Kaye E. Reed

Publications and Research

The Early Pleistocene was a critical time period in the evolution of eastern African mammal faunas, but fossil assemblages sampling this interval are poorly known from Ethiopia ’ s Afar Depression. Field work by the Hadar Research Project in the Busidima Formation exposures (~2.7 – 0.8 Ma) of Hadar in the lower Awash Valley, resulted in the recovery of an early Homo maxilla (A.L. 666-1) with associated stone tools and fauna from the Maka ’ amitalu basin in the 1990s. These assemblages are dated to ~2.35 Ma by the Bouroukie Tuff 3 (BKT-3). Continued work by the Hadar Research Project …

A Study On The Impact Of Diet On Unified Fire Authority Firefighter Performance, Marin Easton

Student Research Symposium

Objective: This project aimed to examine the impact of nutrition knowledge, dietary intake, and physical activity on firefighters’ performance on their routine physical assessment (RPA) Methodology: Members of the Unified Fire Authority were invited via email to participate in this cross-sectional study. Participants (N=92) completed a 40-question survey that assessed specialized diet adherence, dietary intake, nutrition knowledge (using questions from the General Nutrition Knowledge Questionnaire; 1 point for each correct answer [26 points possible]), regular physical activity (International Physical Activity Questionnaire), and perceived physical performance on their RPA. Based on their responses, participants were categorized into one of two groups: …

The Effects Of Strength And Conditioning Training On The Health Of College Students, Brynli Bleak

Student Research Symposium

Does participation in Strength and Conditioning (S & C) classes have a positive effect on the physical, emotional, mental, and social health of college students? This study was designed to measure and explore different markers of physical, mental, emotional, and social health from participants in Strength and Conditioning classes. Class participants were asked to complete a survey to self report the effects of the training on various aspects of their life. Data were analyzed and compared to national averages for college students, and correlation tests were conducted to identify relationships in the reported effects. The study found that no significant …

Sex Differences In The Fecal Microbiome And Hippocampal Glial Morphology Following Diet And Antibiotic Treatment, Anju Saxena, Roberta R M Moran, Meghan R. Bullard, Emma O. Bondy, Matthew Foster Smith, Lainie Morris, Nicaella Fogle, Jagroop Singh, Brendan Jarvis, Tammy Ray, Juhi Saxena, Linnea Ruth Freeman

Open Access Fund Publications

Rising obesity rates have become a major public health concern within the United States. Understanding the systemic and neural effects of obesity is crucial in designing preventive and therapeutic measures. In previous studies, administration of a high fat diet has induced significant weight gain for mouse models of obesity. Interestingly, sex differences in high-fat diet-induced weight gain have been observed, with female mice gaining significantly less weight compared to male mice on the same high-fat diet. It has also been observed that consumption of a high-fat diet can increase neurogliosis, but the mechanism by which this occurs is still not …

Adapt Lake Mead Releases To Inflow To Give Managers More Flexibility To Slow Reservoir Draw Down, David E. Rosenberg

Publications

A 20-year Colorado River drought continues and Lake Mead draws down. As Lake Mead falls through 8 elevation tiers to 1,020 feet (5.7 million acre-feet [maf]), releases drop and mandatory water conservation targets for California, Arizona, Nevada, and Mexico grow to 1.375 maf per year (USBR, 2019). How will different reservoir inflows, releases, and additional water conservation efforts beyond mandatory targets speed or slow Lake Mead draw down, stabilization, and recovery?

40-Years Of Lake Urmia Restoration Research: Synthesis And Next Steps, Marsoud Parsinejad, David E. Rosenberg, Yusuf Alizade Govarchin Ghale, Bahram Khazaei, Sarah Null, Omid Raja, Ammar Safaie, Somayeh Sima, Armin Sorooshian, Wayne A. Wurtsbaugh

Lake Urmia, Iran Collaborations

Public concern over environmental issues such as ecosystem degradation is high. However, restoring coupled human-natural systems requires integration across many science, technology, engineering, management, and governance topics that are presently fragmented. Here, we synthesized 544 peer-reviewed articles published through September 2020 on the desiccation and nascent recovery of Lake Urmia in northwest Iran. We answered nine questions of scientific and popular interest about causes, impacts, stabilization, recovery, and next steps. We find: (1) Expansion of irrigated agriculture, dam construction, and mismanagement impacted the lake more than temperature increases and precipitation decreases. (2) Aerosols from Lake Urmia's exposed lakebed are negatively …

Genetic Characterization Of Resistance To Bacterial Panicle Blight And Sheath Blight In Rice Using Qtl Linkage Analysis And Qtl-Seq, John Christian E. Ontoy

LSU Master's Theses

Bacterial panicle blight (BPB) and sheath blight (SB) are major diseases of rice causing significant yield reduction in conducive conditions. Few cultivars have shown reliable resistance to these diseases. In this study, a recombinant inbred line (RIL) population derived from the U.S. cultivars Jupiter (moderately resistant) and Trenasse (susceptible) was investigated to identify genomic regions associated with BPB and SB resistance. Phenotypes in BPB and SB, as well as days to heading (DH), of the RIL population were evaluated in the field. DH was found to be correlated to BPB and SB diseases. BPB was also positively correlated to SB. …

Hsp90-Cdc37 Functions As A Chaperone For The Oncogenic Fgfr3-Tacc3 Fusion, Tao Li, Farideh Mehraein-Ghomi, M Elizabeth Forbes, Sanjeev V Namjoshi, E Ashley Ballard, Qianqian Song, Ping-Chieh Chou, Xuya Wang, Brittany C Parker Kerrigan, Frederick F Lang, Glenn Lesser, Waldemar Debinski, Xuejun Yang, Wei Zhang

Faculty, Staff and Student Publications

The FGFR3-TACC3 (F3-T3) fusion gene was discovered as an oncogenic molecule in glioblastoma and bladder cancers, and has subsequently been found in many cancer types. Notably, F3-T3 was found to be highly expressed in both untreated and matched recurrence glioblastoma under the concurrent radiotherapy and temozolomide (TMZ) treatment, suggesting that targeting F3-T3 is a valid strategy for treatment. Here, we show that the F3-T3 protein is a client of heat shock protein 90 (HSP90), forming a ternary complex with the cell division cycle 37 (CDC37). Deprivation of HSP90 or CDC37 disrupts the formation of the ternary complex, which destabilizes glycosylated …

Brain Charts For The Human Lifespan, R. A. I. Bethlehem, J. Seidlitz, S. R. White, J. W. Vogel, K. Anderson, C. Adamson, S. Adler, G. S. Alexopoulos, E. Anagnostou, John Blangero

School of Medicine Publications

Over the past few decades, neuroimaging has become a ubiquitous tool in basic research and clinical studies of the human brain. However, no reference standards currently exist to quantify individual differences in neuroimaging metrics over time, in contrast to growth charts for anthropometric traits such as height and weight1. Here we assemble an interactive open resource to benchmark brain morphology derived from any current or future sample of MRI data (http://www.brainchart.io/). With the goal of basing these reference charts on the largest and most inclusive dataset available, acknowledging limitations due to known biases of MRI studies …

Sex And Starvation Influences Latrotoxin Expression In The Brown Widow Spider, Mattie Harris

Honors College Theses

Widow spiders (genus Latrodectus) are well-known for their potent venom. Seven latrotoxin proteins constitute the main components of widow spider venom. The vertebrate specific (α-latrotoxin) and insect specific (α-latroinsectotoxin) latrotoxins have been well-characterized with respect to structure and function. Regulation of latrotoxin gene expression is not well understood but sex and feeding could be factors influencing production. In this study, I used quantitative qPCR to (1) characterize the expression patterns of both the insect and vertebrate specific latrotoxins in male and female brown widow spiders (Latrodectus geometricus) to characterize sex-biased expression and to (2) study expression patterns …

Assessing The Influence Of Urban Greening On Urban Arthropods, Brendon Lawrence

Scholars Week

Green Heart is an urban greening experiment in Louisville, KY seeking to create new urban green spaces to ultimately increase air quality and improve human health. Changes in biodiversity in response to urban greening may influence human health via multiple hypothesized pathways including reducing harm (e.g. medicine provisioning), restoring capacities (e.g. reductions in stress), building capacities (e.g. increasing activity outdoors), and causing harm (e.g. zoonotic diseases). Arthropods are one component of diversity that may influence human health via each of these pathways. However, few studies have assessed the influence of Arthropod diversity on human health. Thus, the objective of our …

Nutrient Uptake And Grafting In Ironwood Trees, Tyler Edvall

Student Academic Conference

No abstract provided.