Life Sciences Commons^™

Evaluating Non-Invasive Environmental Methods For Detecting Tropical African Pangolin Species To Inform Conservation Actions, Ichu Godwill Ichu

Theses and Dissertations

Tropical African pangolin species are threatened throughout their range due to habitat loss and illegal take. Limited knowledge on distribution has rendered conservation efforts challenging. Methods commonly used for other wildlife species need to be tested for each pangolin species as each has variable ecologies requiring specific detection and monitoring techniques. This thesis evaluates the efficacy of two non-invasive environmental methods for detecting tropical African pangolin species, and consists of two complementary studies; a proof of concept study using soil sourced eDNA from a white-bellied pangolin enclosure in the Columbus Zoo, Ohio, to detect the species, and a field study …

Methods And Tools To Improve Performance Of Plant Genome Analysis, Drew Ferrell

Theses and Dissertations

Multi -omics data analysis and integration facilitates hypothesis building toward an understanding of genes and pathway responses driven by environments. Methods designed to estimate and analyze gene expression, with regard to treatments or conditions, can be leveraged to understand gene-level responses in the cell. However, genes often interact and signal within larger structures such as pathways and networks. Complex studies guided toward describing dynamic genetic pathways and networks require algorithms or methods designed for inference based on gene interactions and related topologies. Classes of algorithms and methods may be integrated into generalized workflows for comparative genomics studies, as multi -omics …

Assessing Non-Uniform Inbred And Hybrid Rice (Oryza Sativa) Population For Replant Decisions, Thomas William Eubank Iv

Theses and Dissertations

Optimal plant populations are critical in achieving high rice yields. The purpose of this research was to investigate yield penalties associated with inadequate, non-uniform plant populations for modern rice cultivars. Research was conducted at the Delta Research and Extension Center in Stoneville, MS, in 2019, 2020 and 2021. In one study, inbred, herbicide-resistant ‘CL153’ was drill-seeded at 73-18 kg ha^-1 after being mixed with inbred, herbicide-susceptible ‘Rex’ at 0-55 kg ha^-1. Likewise, hybrid, herbicide-resistant cultivar ‘CL XL7521’ was seeded at 29-8 kg ha^-1 mixed with hybrid, herbicide-susceptible ‘XP753’ at 0-21 kg ha^-1. At the …

Effects Of Anthropogenic Global Change On A Grassland Prairie Community, Carter Wolff

Theses and Dissertations

Anthropogenic global change is altering food web dynamics. Global change comprises factors, like temperature, sound, light and more. In this dissertation, I evaluate how two factors, sound and temperature, alter prairie communities. In Chapter 1, I test if sound influences grasshopper respiration rate, thereby altering diet. Some sound frequencies increased grasshopper respiration rate while others decreased respiration rate. Frequencies that elevated respiration rate led to grasshoppers consuming more carbohydrate-rich foods compared to protein-rich foods. This diet change stems from a carbon deficit due to increased respiration rate and could act as a mechanism by which sound pollution indirectly alters plant …

Phenotypic Evaluation Of Energycane (Saccharum Spp.) Genotypes In Northcentral Mississippi, Wyatt Armistead Eason

Theses and Dissertations

As fossil fuel supplies decrease and concerns of climate change increase, the search for alternative sources of fuel has pushed biomass crops to the forefront of discussion. Saccharum spontaneum readily hybridizes with commercial sugarcane and lends cold tolerance and greater yields to the hybrid progeny, called energycane. Twenty genotypes were tested against an energycane variety (Ho 02-113) as a control. Two locations were tested: the HH Leveck Animal Research Center (planted in 2019), and the Bearden Dairy Research Center (planted in 2020). The Bearden Dairy Research Center yielded significantly higher than the HH Leveck Animal Research Center regarding dry matter …

Classification Models For 2,4-D Formulations In Damaged Enlist Crops Through The Application Of Ftir Spectroscopy And Machine Learning Algorithms, Benjamin Blackburn

Theses and Dissertations

With new 2,4-Dichlorophenoxyacetic acid (2,4-D) tolerant crops, increases in off-target movement events are expected. New formulations may mitigate these events, but standard lab techniques are ineffective in identifying these 2,4-D formulations. Using Fourier-transform infrared spectroscopy and machine learning algorithms, research was conducted to classify 2,4-D formulations in treated herbicide-tolerant soybeans and cotton and observe the influence of leaf treatment status and collection timing on classification accuracy. Pooled Classification models using k-nearest neighbor classified 2,4-D formulations with over 65% accuracy in cotton and soybean. Tissue collected 14 DAT and 21 DAT for cotton and soybean respectively produced higher accuracies than the …

Nondestructive Assessment Of Flexural And Tensile Properties For Southern Pine Structural Lumber, Marly Gabriela Carmona Uzcategui

Theses and Dissertations

The flexural and tensile properties of visually graded southern yellow pine lumber were modeled. Longitudinal and transverse vibration techniques and proof-loading bending tests were used to assess the flexural and tensile properties of southern pine lumber. The properties evaluated were dynamic modulus of elasticity (dMOE), static modulus of elasticity (E_b), tension modulus of elasticity (E_t), and ultimate tensile stress (UTS). The tensile properties were evaluated in the direction parallel to the grain. This study presents the results of tests conducted on No. 2 2 × 6 and 2 × 10 southern pine lumber of two different …

Determination Of Atlantic Shortfin Mako Shark (Isurus Oxyrinchus) Population Genetic Structure And Comparison Of Mitogenomic Markers, Marissa Mehlrose

All HCAS Student Capstones, Theses, and Dissertations

This study provides the first genomics-level, population structure and genetic diversity assessment of the shortfin mako (Isurus oxyrinchus) shark. The assessments were based on mitogenome sequences from makos distributed throughout the Atlantic Ocean, with Pacific mako mitogenomes used as a comparative outgroup. In addition to complete mitogenome-based analyses, I assessed individual mitochondrial coding gene and non-coding (Control Region) sequences for their comparative resolution properties as markers for revealing matrilineal population structure and genetic diversity. Sequencing of 125 makos in the western and eastern Atlantic across northern and southern hemispheres yielded 92 complete mitogenomes (16,698-16,702 bp) and 33 partial …

Effects Of Localized Irrigation And Fertilizer On Woody Plant Establishment In Degraded Semi-Arid Environments, Holley M. Lund

Theses and Dissertations

Semi-arid native plant communities worldwide are often disturbed either intentionally or incidentally by human activity. In order to restore ecological function after human activities cease, native plant communities need to be restored. Woody plants are important to ecological function for many reasons including reducing erosion and providing food and shelter for wildlife. Unfortunately, woody plant establishment in these areas has proven to be challenging. Direct seeding efforts can be hindered by poor germination and low seedling emergence. To overcome this, seedling transplants are often used in harsh sites. However, transplanted woody seedlings often experience high mortality during the first year, …

Development Of A Rhizobium Seed Coating To Establish Lupine Species On Reclaimed Minelands, Bridget May Calder

Theses and Dissertations

Symbiotic interactions among various organisms are often necessary for one or both individual's survival. These symbiotic relationships must be considered in restoration projects to allow for the successful establishment of the species. Rhizobia are nitrogen-fixing bacteria found in symbiotic relations with legumes. By utilizing this relationship, restoration practitioners can establish native legume species more successfully while repopulating soil microorganisms into degraded soils. Despite the potential benefits a rhizobium inoculant can have on restoration efforts, minimal research has been done to understand the impacts this treatment has on specific species and the systems they are employed within. Our research goal was …

Effect Of Cover Crops, Grazing And Tillage Practices On Soil Microbial Community Composition, Function, And Soil Health In East Central Mississippi Soybean Production System., Namita Sinha

Theses and Dissertations

Integrating crop and livestock is being considered to improve soil health by carbon sequestration. A two-year study (2019-2021) at CPBES in Newton, MS was aimed to evaluate soil microbial diversity in the warm, humid regions, specifically southeastern USA. Amplicons targeting bacterial 16S rRNA genes and fungal ITS2 regions were sequenced. Taxonomic assignment and microbial diversity characterization were performed using QIIME2^®. Soil fungal diversity showed significant differences (alpha diversity, p = 0.031 in yr. 2020 and beta diversity, p = 0.037 in yr. 2021). Canonical Correspondence Analysis (CCA) and Mantel test showed significant influence on fungal diversity due to …

Incorporating Follicle Stimulating Hormone To Stimulate Multiple Corpora Lutea For Embryo Transfer In Beef Cattle, Bryan Carter

Theses and Dissertations

Assisted reproductive technologies, such as estrus synchronization and embryo transfer, can aid producers in improving genetics by increasing the number of progeny produced from elite females. The success of embryo transfer is dependent on a viable, competent embryo and a recipient with a receptive uterine environment. Follicular development and luteinization are pertinent for the recipient to establish a functional corpus luteum (CL) that can produce adequate concentrations of progesterone (P4) and provide a uterine environment conducive for the establishment of pregnancy. The objective of this study was to determine if exogenous follicle stimulating hormone (FSH), would increase the number of …

A Biomechanical Approach To Prevent Falls In Ergonomic Settings, Sachini Kodithuwakku Arachchige

Theses and Dissertations

Introduction: Fall-related injuries are exceptionally prevalent in occupational settings. While endangering the workers’ health, falls cause poor productivity and increased economic burden in the workplace. Hence, identifying these threats and training workers to achieve proper postural control is crucial. Purpose: Study 1: To investigate the ankle joint kinematics in unexpected and expected trip responses during single-tasking (ST), dual-tasking (DT), and triple-tasking (TT), before and after a physically fatiguing exercise. Study 2: To investigate the impact of virtual heights, DT, and training on static postural stability and cognitive processing. Methods: Study 1: Twenty collegiate volunteers (10 males and females, one left …

The Effects Of Moderate And High Temperatures On Growth, Metabolism, And Blood-Oxygen Affinity In Ictalurus Spp., Brandon Gerhart

Theses and Dissertations

Despite the growing use of hybrid catfish (Ictalurus furcatus × I. punctatus) in aquaculture, few studies have investigated the comparative effects of temperature on physiological performance with other cultured catfish. Therefore, growth, metabolism, and blood-oxygen affinity of blue (I. furcatus), channel (I. punctatus), and hybrid catfish were compared at moderate (23°C) and high (33°C) water temperatures. Channel catfish had the greatest growth and were in better condition compared to blue and hybrid catfish. Hybrid catfish standard metabolic rates did not differ from blue or channel catfish, while hybrid catfish had higher maximum metabolic rates, …

The Employability Of State Ffa Degree Recipients In Alabama, Kailee D. Johnson

Theses and Dissertations

Many high schools have shifted from comprehensive graduation exams to a focus on College and Career Readiness. A common method used to determine when a student is college and career ready is a Career Readiness Indicator (CRI). Many CRIs demonstrate a benchmark learning goal for students. Among academic-based CRIs are Career and Technical Education (CTE) industry credentials. Credentials are just one way that agriculture classrooms contribute to student success. This study compiled existing research on CRIs and employability skills in CTE students to determine how the State FFA Degree could potentially fit into this category as well as highlight specific …

Sustainable Sidedress Nitrogen Applications For Early Corn And Cotton Crops Using Small Unmanned Aerial Systems, James Nolan Parker

Theses and Dissertations

Nitrogen run-off from agriculture have been linked to human health problems on a global level. Large-scale conventional producers struggle to redefine themselves as sustainable because reducing nitrogen (N) inputs without justification or validation may lead to severe profit losses. Small unmanned aerial systems (sUAS) sensing may allow for decreased N runoff. Failure to address this problem will exacerbate already excessive N runoff into the Mississippi River and beyond. The purpose of this study was to reduce fertilizer N input using sUAS technology to assess crop canopy needs. In 2020 and 2021, variable rate nitrogen (VRN) side-dress N application maps were …

Determining The Effects Of Elevated Carbon Dioxide On Soil Acidification, Cation Depletion, And Soil Inorganic Carbon And Mapping Soil Carbons Using Artificial Intelligence, Jannatul Ferdush

Theses and Dissertations

Soil carbon is the largest sink and source of the global carbon cycle and is disturbed by several natural, anthropogenic, and environmental factors. The global increase of atmospheric CO₂ affects soil carbon cycling through varied biogeochemical processes. The first chapter is a compilation of current information on potential factors triggering soil acidification and weathering mechanisms under elevated CO₂ and their consequences on soil inorganic carbon (SIC) pool and quality. Soil water content and precipitation were critical factors influencing elevated CO₂ effects on the SIC pool. The second chapter examines a detailed column experiment in which six soils …

Effectiveness Of Food-Grade Coating Treated Nets After Various Drying Methods At Controlling Mite Growth On Dry-Cured Hams, Sadie White

Theses and Dissertations

Tyrophagus putrescentiae, known as the ham mite, is the most difficult pest to control in the dry-cured ham industry. Food-grade propylene glycol coated nets have been studied as an alternative to conventional mite infestation treatments but were heavy and costly to ship. This study aimed to increase the ease of use of the coated nets via drying. Three drying treatments: oven dried for 7 min (OD7m), oven dried for 20 min (OD20m), and counter dried for 24 h (CD24h), were tested to determine the efficacy at controlling mites as well as the mechanical properties of the nets and sensory characteristics …

Effects Of Mass Death On Community Structure And Ecosystem Function, Abby Kimpton Jones

Theses and Dissertations

Death and decomposition are natural processes that are generally well-understood. However, large events of death, such as mass mortality events (MMEs) are increasing in frequency and their impacts on the ecosystem are largely unknown. These events may have both bottom-up effects from increased nutrient input as well as top-down effects from loss of an ecological functional group by the affected population. Different functional MMEs may result in different top-down effects, creating cascading effects. In Chapter 1, I test the hypothesis that scavenger and herbivore simulated MMEs generate novel bottom-up and top-down effects. Results indicate that MMEs have a significant effect …

Soybean Potassium Utilization During Reproductive Growth Stages In Response To Potassium Timing, Potassium Rate, Herbicide Injury, And Desiccation, Tyler Lawrence Richmond

Theses and Dissertations

Potassium (K) deficiency of soybean [Glycine max (Merr.) L.] is common throughout the Mid-Southern region of the United States. Visual symptoms of K deficiency may be absent or subtle during early and mid-reproductive growth stages. Correcting K deficiency in-season is problematic due to limited research. Information regarding soybean yield response to in-season fertilizer K application time is limited to growth stages prior to the onset of R1. Research is also limited on new higher yielding soybean cultivars. The objective of this study was to evaluate influences of in-season K application timing during reproductive growth stages on soybean yield. Field experiments …

The Impacts Of The Covid-19 Pandemic On The Food Security Of Mississippians, Hannah Noel Irwin

Theses and Dissertations

Mississippi leads the United States in food insecurity, with 15.3% of Mississippians experiencing food insecurity in a given year. To determine the impacts of the COVID-19 pandemic on the food insecurity of Mississippians, a survey containing the USDA Household Food Security Questionnaire was distributed to adult Mississippi residents. By employing the USDA Household Food Security Scale and the Foster-Greer-Thorbecke Methodology, this study finds that the food insecurity rate, food insecurity gap, and squared food insecurity gap have worsened in Mississippi since the beginning of the COVID-19 pandemic. Additionally, this study finds that households which were food insecure prior to March …

Assessing Virtual Free-Choice Agricultural Learning: A Mixed Methods Approach, Richard John Kennedy

Theses and Dissertations

Since the mid-19th century, agricultural museums and living history sites, first in Scandinavia and then in the United States and Canada, have provided historical, cultural, and, sometimes, scientific information about farming to visitors. Many of these visitors have been school children on field trips. Assessment of this learning, however, has been scant, particularly within the free-choice style inherent in these types of educational venues. At the same time, assessment of free-choice learning, often analyzed through the Contextual Model of Learning (Falk & Dierking 2000), has taken place at educational sites like zoos, natural history museums, and science centers. This study, …

Utilizing Unmanned Aerial Systems To Sample Insects In Soybean, Marvin Merkl

Theses and Dissertations

To overcome some limitations of manual insect sampling in soybeans, an unmanned aerial vehicle (UAV) sampling platform was developed that could collect insects in a sweep net attached to the bottom of a UAV. Before this UAV sampling platform can be used to make management decisions, correlations with manual sweep net and/or drop cloth sampling methods are needed. This will allow action thresholds for the various pests to be calculated for the UAV sampling platform. To make the correlations, 87 soybean fields were sampled during 2020 and 2021 with each of 4 sampling methods, a UAV travelling 50-m, the same …

The Relationship Between Leadership Preference Of County Level Personnel Within University Of Missouri Extension And Their Level Of Employee Engagement, Jason C. Morris

Theses and Dissertations

The purpose of this study was to describe the perceived leadership style of the University of Missouri Extension Service county staff and their level of engagement and study the relationship between variables. Administrators with University of Missouri Extension Service can utilize this information to better serve Extension Staff and ultimately people throughout the state of Missouri.

The Vannsimpco Leadership Survey was used to measure the perceived leadership style of county level staff. The Utrecht Work Engagement Scale (UWES) was used to measure work engagement on three constructs: vigor, absorption, and dedication engagement. Demographic characterizes of county level staff was also …

Inosine 5’- Monophosphate Derived Umami Flavor Intensity Of Beef Determination By Electrochemistry And Chromatography, Kezia Virellia To

Theses and Dissertations

The umami sensation contributes to beef flavor and acceptability. Inosine 5’- monophosphate (IMP) was the most abundant nucleotide in meat known to impart umami taste which thus far had been overlooked in meat flavor studies. The objectives of this study were to determine the umami taste threshold of inosine 5'-monophosphate (IMP), the effects of spiking IMP on the sensory descriptive attributes of various USDA graded beef strip steaks, and to differentiate beef by IMP content using electrochemistry. USDA Prime, Choice, and Select steaks were spiked with 0.3 and 0.6 mM IMP and analyzed chemically and organoleptically. Liquid chromatography-mass spectrometry and …

Improvement Value Of Forest Resources By Use Of Cottonseed Protein Meal As A Bio-Based Wood Adhesive For Hardwood Plywood Products, Edward David Entsminger

Theses and Dissertations

Literature shows that production of cottonseed adhesives is feasible to develop an environmentally friendly and competitive bio-based wood adhesive. Defatted cottonseed and water-washed cottonseed meals were prepared from glandless cottonseed and were used in adhesive formulations to produce three-ply yellow poplar (Liriodendron tulipifera) plywood panels as the first objective. These two cottonseed meals were compared with the properties of plywood panels made with an adhesive formulated from a commercial soybean meal, as a control. Adhesive resins were prepared from each protein meal with sodium metabisulfite (Na2S2O5) and one of two polyamido-amine-epichlorohydrin (PAE) wet strength agents, and the plywood panels were …

Assessing The Flood Tolerance, Physiological Mechanism, And Nutrient Mitigation Potential Of Short Rotation Woody Crops Planted On Seasonally Flooded Marginal Land Of The Lower Mississippi Alluvial Valley, Thu Ya Kyaw

Theses and Dissertations

In 2019, the Mississippi River watershed had a record-long flooding, which was comparable with the 1927 Great Mississippi River Flood. This study leveraged this flooded condition to assess the flood tolerance of eastern cottonwood (Populus deltoides) and black willow (Salix nigra) planted as short rotation woody crops (SRWCs) on seasonally flooded marginal land of the Lower Mississippi Alluvial Valley (LMAV) in 2018. The survival/mortality prediction models developed by using hydrologic and environmental variables suggested that only high flood depth affected the survival of black willow. However, eastern cottonwood was threatened by flood depth, flood duration, and …

Novel Silviculture Practices For Non-Industrial Private Forest Landowners, Darcey Alyce Collins

Theses and Dissertations

Many landowners in the southeastern United States have invested in southern yellow pine plantations and managed them using traditional management schemes. However, non-industrial private forest landowners often have goals and challenges that make traditional management less desirable or possible. This thesis contains two projects that offer potential solutions for these landowners. The first project is a management option for landowners with small parcels that wish to manage pine plantations and proposes low-density management paired with crown lifting for the production of saw logs without commercial thinning entries. The second proposes a method for incorporating mixed-species management into already existing plantations. …

Effects Of The In Ovo Administration Of The Poulvac E. Coli Vaccine In Layer Hatching Eggs On Hatchability, Hatchling Characteristics, And Early Posthatch Chick Performance, Lauren L. Lindsey

Theses and Dissertations

Avian Pathogenic E. coli can lead to infections in laying hens and wide-spread economic problems in the table egg industry. A vaccine widely used to combat APEC is the Poulvac E. coli vaccine, which is normally applied via spray vaccination. Application of this vaccine via in ovo injection has not been examined. The purpose of this series of studies was to determine the effects of injecting 50 μL of different dosages of the PECV into Hy-Line W-36 layer hatching eggs on the hatchability and characteristics of the hatchlings, and on the livability and characteristics of the chicks through 21 d …

Behavioral And Histological Inflammatory Analysis Of A Single, Mild Traumatic Brain Injury And Repeated Subconcussive Brain Injury Using A Rodent Model., Anna Marie Clay

Theses and Dissertations

Subconcussive (SC) impacts have become a growing concern within the neuroscience community regarding the immediate and long-lasting effects of sports-related injuries. While a single low-level impact, i.e., a subconcussion, may not cause cerebral perturbations, it has been increasingly recognized that repeated SC exposure can induce deleterious effects. Therefore, determining the lower limits of systematic perturbation resulting from multiple SC impacts is of critical importance in expanding our understanding of cerebral vulnerability and recovery. Currently, there is a lack of correlation between a mild traumatic brain injury (mTBI) and repeated SC impacts with respect to injury biomechanics. Moreover, the cumulative threshold …