Life Sciences Commons^™

Quantification Of Spent Coffee Ground Extracts By Roast And Brew Method, And Their Utility In A Green Synthesis Of Gold And Silver Nanoparticles, Brian G Yust, Niny Z Rao, Evan T Schwarzmann, Madisyn H Peoples

College of Life Sciences Faculty Papers

Nanotechnology has become increasingly important in modern society, and nanoparticles are routinely used in many areas of technology, industry, and commercial products. Many species of nanoparticle (NP) are typically synthesized using toxic or hazardous chemicals, making these methods less environmentally friendly. Consequently, there has been growing interest in green synthesis methods, which avoid unnecessary exposure to toxic chemicals and reduce harmful waste. Synthesis methods which utilize food waste products are particularly attractive because they add value and a secondary use for material which would otherwise be disposed of. Here, we show that spent coffee grounds (SCGs) that have already been …

Behavioral Responses Of A Parasitoid Fly To Rapidly Evolving Host Signals, E. Dale Broder, James H. Gallagher, Aaron W. Wikle, Cameron P. Venable, David M. Zonana, Spencer J. Ingley, Tanner C. Smith, Robin M. Tinghitella

Biological Sciences: Faculty Scholarship

Animals eavesdrop on signals and cues generated by prey, predators, hosts, parasites, competing species, and conspecifics, and the conspicuousness of sexual signals makes them particularly susceptible. Yet, when sexual signals evolve, most attention is paid to impacts on intended receivers (potential mates) rather than fitness consequences for eavesdroppers. Using the rapidly evolving interaction between the Pacific field cricket, Teleogryllus oceanicus, and the parasitoid fly, Ormia ochracea, we asked how parasitoids initially respond to novel changes in host signals. We recently discovered a novel sexual signal, purring song, in Hawaiian populations of T. oceanicus that appears to have evolved because it …

Pengaruh Konsentrasi Benzylaminopurine Terhadap Pertumbuhan Eksplan Tunas Aksilar Rami Klon Lokal Wonosobo Secara In Vitro, Anne Nuraini, Eva Aprilia, Murgayanti, Asri Peni Wulandari

Jurnal Kultivasi

Saat ini, perbanyakan rami menggunakan rizoma sebagai bahan tanamnya, tetapi dalam produksinya membutuhkan waktu yang lama dan sebagai bahan tanam umur simpannya singkat. Kultur jaringan merupakan salah satu teknologi untuk mendapatkan bahan tanam yang seragam dan sehat dalam waktu yang singkat. Tujuan dilakukannya penelitian ini adalah untuk mengetahui pengaruh konsentrasi BAP terhadap pertumbuhan tunas aksilar pada rami klon lokal Wonosobo dan konsentrasi mana yang memberikan pengaruh terbaik. Percobaan dilakukan di Laboratorium Kultur Jaringan, Teknologi Benih, Fakultas Pertanian, Universitas Padjadjaran. Nodus batang dari rami klon lokal Wonosobo diklturkan pada media MS dengan penambahan berbagai konsentrasi BAP selama 8 minggu dan diamati …

Respon Perkecambahan Biji Aren Terhadap Larutan Pupuk Organik Cair Dan Waktu Perendaman, Rosi Widarawati, Totok Agung Dwi Haryanto, Ruth Feti Rahayuniati

Jurnal Kultivasi

Peningkatan kualitas kecambah dapat dibantu dengan seed priming, seperti perendaman dalam larutan pupuk organik cair. Penelitian bertujuan untuk mengetahui: pengaruh berbagai bahan dan konsentrasi seed priming terhadap perkecambahan biji aren (Arenga pinnata (Wurmb.) Merr.), pengaruh waktu perendaman terhadap perkecambahan biji aren, dan interaksi antara berbagai konsentrasi dan waktu perendaman pada perkecambahan aren. Penelitian dilaksanakan di Dusun 2 Desa Pasir Kulon Kecamatan Karanglewas Kabupaten Banyumas Jawa Tengah dengan ketinggian tempat 140 m di atas permukaan laut pada September 2020 sampai Maret 2021. Rancangan percobaan yang digunakan dalam penelitian adalah Rancangan Acak Kelompok (RAK) faktorial dengan dua faktor perlakuan dan diulang tiga …

Pengendalian Penyakit Busuk Buah Kakao Menggunakan Trichoderma Dan Pupuk Kalium, Sunjaya Putra, Yulius Ferry, Rita Harni

Jurnal Kultivasi

Kerugian akibat serangan penyakit busuk buah kakao (BBK) mencapai 40-100%, produktivitas hanya 669,9 kg/ha/tahun. Upaya pengendalian dapat dilakukan melalui penggunaan cendawan antagonis Trichoderma dan peningkatan dosis pupuk Kalium. Penelitian bertujuan menguji efektivitas Trichoderma viride dan pupuk K dalam mengendalikan penyakit BBK. Penelitian dilakukan pada Januari sampai Desember 2019 di kebun kakao rakyat (Klon CSA 6) berumur 10 tahun dengan tanaman pelindung Gliricidia sp. Desa Suka Bandung, Kecamatan Natar, Kabupaten Lampung Selatan. Penelitian menggunakan rancangan acak kelompok dengan 12 perlakuan dan 3 ulangan. Macam perlakuan yaitu fungisida Trichoderma viride, fungisida kimia (mancozeb), pupuk KCl 100 g/phn, dosis KCl 110 g/phn, dosis …

Meeting Minutes, August 11th, 2022, National Smokejumper Association Board Of Directors

National Smokejumper Association Meeting Minutes

Agenda: 1. Call to Order; 2. Reunion Update; 3. Agenda and Consent Agenda; 4. National Hot Shot Association (NHSA) Report; 5. Financials and Treasurer’s Report (HO 5a and 5b); 6. Historical Preservation at Eastern Washington University (HO 6); 7. NSA Future (HO 7); 8. Forest Service Museum Proposed MOU (HO 8a, 8b, 8c, and 8d); 9. NSA Website (HO 9a and 9b); 10. Soliciting Grants and Gifts (HO 10c); 11. Investment Committee (HO 11a); 12. Nominations Committee (HO 12); 13. Legacy Fund; 14. Budget (HO 13); 15. Report from Liaison to Active Jumpers; 16. Tramps Trail Program; 17. Facebook; 18. …

Relationships Between Mercury And Trophic Level In Nine Coastal Pelagic Fishes Off Southeastern Florida, Emily Akins

All HCAS Student Capstones, Theses, and Dissertations

State and federal agencies have issued consumption advisories for various fish species for many years, including in Florida. Upper-level predatory fish, such as tunas and mackerels, are especially popular with anglers but are susceptible to high levels of mercury through bioaccumulation and biomagnification. This study used two data sets over two time periods, 2010-2012 and 2020-2021, to compare mercury and trophic level relationships in nine coastal pelagic fishes is Southeastern Florida. As these species are popular in recreational fisheries, charter and tournament catches formed the base of the samples that were analyzed for total mercury along with carbon and nitrogen …

Open Source Solutions In Experimental Design: An Introduction To The Symposium, Richelle L. Tanner, Kirt L. Onthank

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

The Open Science movement has increased dramatically in popularity with deserved calls to action around transparency, access to resources, and inclusion in our field. However, its practical applications within experimental design have been slow to uptake, with researchers unsure where to even start with the dizzying array of open source hardware and software solutions available. The perceived time investment and unknown cost, especially in implementing open source hardware, has stagnated the implementation of inexpensive experimental solutions, but we sought to increase awareness to lower the barrier to participation in this space. While there are countless technical and financial advantages to …

A Comprehensive Search Of Non-Canonical Proteins In Non-Small Cell Lung Cancer And Their Impact On The Immune Response, Ehsan Irajizad, Johannes F Fahrmann, James P Long, Jody Vykoukal, Makoto Kobayashi, Chuan-Yih Yu, Fu Chung Hsiao, Nikul Patel, Soyoung Park, Qian Peng, Jennifer B Dennison, Taketo Kato, Mei Chee Tai, Ayumu Taguchi, Humam Kadara, Ignacio I Wistuba, Hiroyuki Katayama, Kim-Anh Do, Samir M Hanash, Edwin J Ostrin

Faculty, Staff and Student Publications

There is substantial interest in mining neoantigens for cancer applications. Non-canonical proteins resulting from frameshift mutations have been identified as neoantigens in cancer. We investigated the landscape of non-canonical proteins in non-small cell lung cancer (NSCLC) and their induced immune response in the form of autoantibodies. A database of cryptoproteins was computationally constructed and comprised all alternate open reading frames (altORFs) and ORFs identified in pseudogenes, noncoding RNAs, and untranslated regions of mRNAs that did not align with known canonical proteins. Proteomic profiles of seventeen lung adenocarcinoma (LUAD) cell lines were searched to evaluate the occurrence of cryptoproteins. To assess …

The First Record Of Scorpiops Bhutanensis Tikader & Bastawade, 1983 From China, With The First Report Of Its Female (Scorpiones: Scorpiopidae), He-Yu Lv, Zhi-Yong Di

Euscorpius

The first record of Scorpiops bhutanensis Tikader & Bastawade, 1983 from China (Xizang). An emended diagnosis, detailed redescription, and illustrations of the species are provided. Its female is reported here for the first time.

The Role Of Ot In Adapted Crossfit, Emily Adams

Student Capstone Papers

Background: A quarter of adults are diagnosed with a disability in the United States, with almost half considered being inactive (CDC, 2020). The population of persons with disabilities (PWD) are at higher risk for secondary health concerns due to a sedentary lifestyle (Diaz et al., 2019). CrossFit recently implemented their first adaptive division in 2021 (CrossFit, LLC, 2022), however there continue to be barriers preventing PWD from participating in CrossFit.

Problem/Purpose: PWD may experience limited access to CrossFit due to structural and social barriers to access equipment and participating in movements. The purpose of this project was to …

An Evaluation Of Ethograms Measuring Distinct Features Of Enrichment Use By Captive Chimpanzees (Pan Troglodytes), Julia L. Greeson, Kara I. Gabriel, John B. Mulcahy, Bonnie King Hendrickson, Susan D. Lonborg, Jay C. Holloway

Student Published Works

Environmental enrichment provides mental stimulation and minimizes abnormal behaviors in captive animals. In captive chimpanzees, individual animals may vary in the ways in which they benefit from enrichment or use enrichment devices, so investigating nuances in enrichment use may improve the welfare of captive chimpanzees. In the current study, three ethograms measuring distinct features of enrichment use (i.e., enrichment object, manipulation behavior, and social context) were evaluated by coding videos of captive chimpanzees (Pan troglodytes) at Chimpanzee Sanctuary Northwest in Cle Elum, WA. A total of 732 min and 58 s of video footage was coded from a larger video …

Subtype-Selective Positive Modulation Of KCa2.3 Channels Increases Cilia Length, Young-Woo Nam, Rajasekharreddy Pala, Naglaa Salem El-Sayed, Denisse Laren-Henriquez, Farideh Amirrad, Grace Yang, Mohammad Asikur Rahman, Razan Orfali, Myles Downey, Keykavous Parang, Surya M. Nauli, Miao Zhang

Pharmacy Faculty Articles and Research

Small-conductance Ca²⁺-activated potassium (K_Ca2.x) channels are gated exclusively by intracellular Ca²⁺. The activation of K_Ca2.3 channels induces hyperpolarization, which augments Ca²⁺ signaling in endothelial cells. Cilia are specialized Ca²⁺ signaling compartments. Here, we identified compound 4 that potentiates human K_Ca2.3 channels selectively. The subtype selectivity of compound 4 for human K_Ca2.3 over rat K_Ca2.2a channels relies on an isoleucine residue in the HA/HB helices. Positive modulation of K_Ca2.3 channels by compound 4 increased flow-induced Ca²⁺ signaling and cilia length, while negative …

Scotus Invalidates Obama Clean Power Plan, J. David Aiken

Cornhusker Economics

On June 30, 2022, the Supreme Court of the United States (SCOTUS) ruled in the case of West Virginia v. EPA that the US Environmental Protection Agency (EPA) could not implement the 2016 Obama administration Clean Power Plan (CPP). This article briefly discusses the CPP, the CPP litigation, the Court's opinion in West Virginia v. EPA, and what the decision means for Biden administration climate policy.

Regulation Of T Cell Activation By The Cd5 Co-Receptor And Altered Peptides, Characterization Of Thymidine Kinase-Specific Antibodies, And Integrating Genomics Education In Society, Kiara Vaden Whitley

Theses and Dissertations

Helper T cells (Th) are a vital component of the immune system responsible for directing other immune cells to eliminate pathogens and cancer. Specifically, Th cells facilitate B cell and cytotoxic T cell (Tc) activation and recruitment and enhance their function against cancer and infectious diseases. Th cells are a valuable resource for improving Tc responses in cancer treatment and have become a focus of immunotherapeutic research. While it is increasingly clear that helper T cells serve an important role, the details about which entities produce an effective Th cell response remain unclear. CD5 is a T cell co-receptor that …

Spatial Variation Of False Map Turtle (Graptemys Pseudogeographica) Bacterial Microbiota In The Lower Missouri River, United States, Joseph D. Madison, Madeline M. Butterfield, Drew R. Davis, Jacob L. Kerby

School of Earth, Environmental, & Marine Sciences Faculty Publications

Turtle populations around the world are continually confronted with changing environments that affect their ecology and conservation status. Among freshwater turtles, population dynamics are thought to be mediated by complex yet often cryptic causes. One recent direction of focus in addressing these causes is the turtle-associated microbiota. In turtles, the gut- associated microbiota is of exceptional interest due to its continual association with host species under changing conditions. Diet-based fluctuations and changes in microbial diversity may correspond to varying external environments at both the individual and population level. Environmental responses are of particular interest due to the anthropogenic changes that …

Digestibility Of Wheat Alpha-Amylase/Trypsin Inhibitors Using A Caricain Digestive Supplement, Angéla Juhász, Mitchell G. Nye-Wood, Gregory J. Tanner, Michelle L. Colgrave

Research outputs 2022 to 2026

Wheat is a major source of nutrition, though in susceptible people it can elicit inappropriate immune responses. Wheat allergy and non-celiac wheat sensitivity are caused by various wheat proteins, including alpha-amylase trypsin inhibitors (ATIs). These proteins, like the gluten proteins which can cause celiac disease, are incompletely digested in the stomach such that immunogenic epitopes reach the lower digestive system where they elicit the undesirable immune response. The only completely effective treatment for these immune reactions is to eliminate the food trigger from the diet, though inadvertent or accidental consumption can still cause debilitating symptoms in susceptible people. One approach …

Impacts On Fast-Start Performance: How Do Group Size And Habitat Degradation Alter The Escape Behavior Of A Schooling Coral Reef Fish?, Monica D. Bacchus

All HCAS Student Capstones, Theses, and Dissertations

Escaping predation is essential for species survival, but prey must effectively match their response to the perceived threat imposed by a predator. Fish evaluate their surroundings using several sensory stimuli, including olfactory, visual, auditory, and mechanical cues. A range of taxa use the fast-start response to evade predators, including fishes, sharks, and larval amphibians. While the fast-start response (rapid bursts of swimming) is extensively studied in solitary fishes, the factors that mediate the collective escape response in schools of fish have historically been investigated far less. To address this knowledge gap, the collective escape behavior and individual escape performance of …

Preliminary Trials To Assess Bycatch Reduction Potential For Deep-Set Pelagic Longline Gear In The U.S. Atlantic Fishery, Sean T. Wilms

All HCAS Student Capstones, Theses, and Dissertations

The U.S. Atlantic pelagic longline fishery uses a shallow-set gear configuration to primarily target swordfish (Xiphias gladius) and yellowfin tuna (Thunnus albacares) at depths of around 75-100 m. However, the fishery has been the subject of several bycatch reduction regulations due to incidental catch and mortality of sea turtles, seabirds, marine mammals, and istiophorid billfishes. An alternative deep-set technique, used by the U.S. pelagic longline fishery based in Hawaii and several non-U.S. fleets, differs from the shallow-set pelagic longline gear by deploying a greater length of mainline per unit distance using a mechanical line shooter, resulting in a deeper catenary …

Purifications Of Iraqi Petroleum Using Ceramic Ball Nano Cobalt Nickel Ferrite Filter, Huda Jabbar, Enas Muhi, Tahseen Hussien

Karbala International Journal of Modern Science

Iraqi petroleum, especially from the Al-Ahdab, has a big problem resulting from its high percentage of heavy metals. In this paper, heavy metals were reduced or removed from Iraqi petroleum using a Ceramic Ball Nano Cobalt Nickel Ferrite Filter (BCNF), synthesized by combining kaolin and palm frond in a 30% ratio with Co0.8Ni0.2Fe2O4 nanoparticles in a various ratios (5, 10, 15, and 20%). The sol-gel technique prepared Co0.8Ni0.2Fe2O4 nanoparticles. The structure and magnetic properties of the material are described using X-RD, FT-IR, and VSM techniques. In addition, the water ab-sorption ratio and apparent porosity were assessed. The results show that …

Studying The Physical And Biological Characteristics Of Denture Base Resin Pmma Reinforced With Zro2 And Tio2 Nanoparticles, Fatin A. Asim, Entessar H.A. Al-Mosaweb, Wafaa A. Hussain

Karbala International Journal of Modern Science

Polymethyl methacrylate (PMMA) suffers from poor mechanical properties that limit its application in the bio-medical field. In this study, PMMA was reinforced with zirconium dioxide (ZrO2) and titanium dioxide (TiO2) nanopar-ticles; subsequently, the hardness, porosity, biocompatibility, bacterial adhesion, and colonization of the reinforced PMMA with various oxide nanoparticles were characterized. The results of this study indicated that reinforced material inhibits bacterial growth and decreases bacterial adhesion by decreasing porosity and increasing PMMA hardness. Based on the findings, 3 wt% PMMA-ZrO2 and 3 wt% PMMA-ZrO2 -TiO2 composites significantly inhibited bacterial growth and adherence while maintaining hemolysis PT and INR and enhancing …

Skin Lesion Segmentation Based On U-Shaped Network, Muna Khalaf, Ban N. Dhannoon

Karbala International Journal of Modern Science

Skin lesion segmentation is an essential step toward accurate skin lesion diagnosis. The need to automate Skin lesion segmentation on the one hand, and the challenges it faces, on the other hand, have made it a growing area of research and focus. Automation of skin lesion segmentation helps reduce the effort and time needed for diagnosis and treatment and helps make better utilization of available data and shared experiences. The challenges faced by the automation of skin lesion segmentation can be broadly defined by (but not limited to); variations in texture, shape, and size for skin lesions and the low …

C60 Hydrofullerene Induced Autophagy And Ameliorated Gfap In H2o2 Treated Human Malignant Glioblastoma U-373 Cell Line, Aryan M. Faraj, Can A. Agca, Victor S. Nedzvetsky, Artem A. Tykhomyrov

Karbala International Journal of Modern Science

Glioblastoma is one of the most combative astrocytoma that is resistant to chemotherapy and radiotherapy. This resistance makes it very difficult to treat. However, researches have shown that nanoparticles especially C60 hydrofullerene have antioxidant and anticancer activity. The effect of C60 hydrofullerene in cancer has been extensively studied; however, the potential regulation of autophagy and modulation of the Glial Fibrillary Acidic Protein (GFAP) gene has not been addressed in glioblastomas. Glioblastoma U-373 cell was treated with 0.5 µM of C60 hydrofullerene and/or 1 mM of hydrogen peroxide (H2O2) for 24 hours. This study demonstrated that C60 hydrofullerene and H2O2 significantly …

Improving Prediction Of Arabic Fake News Using Fuzzy Logic And Modified Random Forest Model, Tahseen A. Wotaifi, Ban N. Dhannoon

Karbala International Journal of Modern Science

Throughout the last few years, the world is witnessing the so-called age of social media, as there is a complete dependence on these sites for following up on events and activities. The problem is that the misinformation or fake news is always released at the appropriate time, so this false news spreads quickly and takes a very wide resonance. Although several studies are performed to determine English fake news, the identification of Arabic misinformation remains underdeveloped. This study aims to build an improved learning model for detecting fake news in the Arabic language. Unlike previous studies that depended on analyzing …

Synthesized Zinc Nanoparticles Via Pulsed Laser Ablation: Characterization And Antibacterial Activity, Sahar Naji Rashid, Kadhim A. Aadim, Awatif Sabir Jasim, Arshad Mahdi Hamad

Karbala International Journal of Modern Science

The pulsed laser ablation of a metallic target in the liquid (PLAL) is a modern and good method for creating a variety of nanomaterials that have piqued the interest of researchers in the last decade. It is used in this work to prepare zinc na-noparticles and zinc oxide nanoparticles (ZnPNs and ZnO NPs) using Nd: YAG laser with the two wavelengths (532 nm) and (355 nm) using the energies (600 mJ) and (500 mJ) respectively, and the number of the pulse (500, 600, 700, 800, and 900 Pulses); for each wavelength used in this work. The properties of the prepared …

A Successful Elimination Of Indonesian Sars-Cov-2 Variants And Airborne Transmission Prevention By Cold Plasma In Fighting Covid-19 Pandemic: A Preliminary Study, Muhammad Nur, Chairul A. Nidom, Setyarina Indrasari, Arif N. M. Ansori, Mohamad Y. Alamudi, Astria N. Nidom, Sumariyah Sumariyah, Eva Sasmita, Eko Yulianto, Andi W. Kinandana, Anwar Usman, Muhammad K. J. Kusala, Irine Normalina, Reviany V. Nidom

Karbala International Journal of Modern Science

Global infection and mortality rates have soared to millions due to SARS-CoV-2 human-to-human transmission from via droplets which then declared as pandemic. This study examined the created cold plasma equipment (CPE) effectiveness in reducing COVID-19 transmission in a confined space. CPE sucked air using a fan in a test chamber then pushed it into a cold plasma reactor. The results indicated that it was able to terminate all SARS-CoV-2 variants along with bacteria and fungi indoors by keeping it turned on for 30 minutes’ minimum. CPE was proven as safe and effective to hinder virus transmission with the acceptable ozone …

The Distinction Of Logical Decision According To The Model Of The Analysis Of Brain Signals (Eeg), Akeel Abdulkareem Al-Sakaa, Zaid H. Nasralla, Mohsin Hasan Hussein, Saif A. Abd, Hazim Alsaqaa, Kesra Nermend, Anna Borawska

Karbala International Journal of Modern Science

Recently, brain signal patterns have been recruited by researchers in different life activities. Researchers have studied each life activity and how brain signal patterns appear. These patterns could then be generalised and used in different disciplines. In this paper, we study the brain state during decision making in a lottery experiment. An EEG device is used to capture brain signals during an experiment to extract the optimal state for logical decision making. After collecting data, extracting useful information and then processing it, the proposed method is able to identify rational decisions from irrational ones with a success rate of 67%.

Functional Traits’ Annual Variation Exceeds Nitrogen-Driven Variation In Grassland Plant Species, George R. Wheeler, Chad Brassil, Johannes M. H. Knops

School of Biological Sciences: Faculty Publications

Effective application of functional trait approaches to ecological questions requires understanding the patterns of trait variation within species as well as between them. However, few studies address the potential for intraspecific variation to occur on a temporal basis and, thus, for trait-based findings to be contingent upon sampling year. To quantify annual variation in the functional traits of grassland plant species, we measured specific leaf area, leaf dry matter content, plant height, and chlorophyll content in 12 shortgrass prairie plant species. We repeated these measurements across 4 years, both in long-term nitrogen addition plots and in corresponding control plots. Three …

Application Of Organic Fertilizers For Lawn Establishment And Maintenance In Louisiana, Matthew Lambert

LSU Master's Theses

Landscape contractors are increasingly interested in organic products for Louisiana's residential and commercial turfgrass areas. However, the use, motivations, and barriers to adopting organic practices in the commercial turfgrass and landscape industry are undocumented. A survey with Louisiana Turfgrass Association (LTA) members to gauge their perceptions on current and future use of organic products in Louisiana was performed; and a field trial was conducted to evaluate organic fertilizers during turfgrass establishment. Substantial majorities of turfgrass professionals are currently applying organic fertilizers and believe organic product use will increase due to consumer demands, potential governmental regulation, and the belief that they …

Evaluating Functional Zoning Based On Site Index To Achieve Competing Objectives Held By Family Forest Owners On Southern U.S. Pine Forest Tracts, Bradley S. Resch

Theses and Dissertations

Family forests comprise a significant portion of total forest lands in the southern United States and their owners frequently have multiple, competing objectives. This research evaluated the effectiveness of functional zoning based on site index on forest sizes relevant to family forest owners. A total of fifty family forests were randomly selected from counties in the East Texas Pineywoods region. Timber production and quail habitat were used as proxies for competing objectives. It was found that 80% of family forest parcels had sufficient site index heterogeneity to benefit from functional zoning. For forest parcels that could benefit from functional zoning, …