Life Sciences Commons^™

The Splice Index As A Prognostic Biomarker Of Strength And Function In Myotonic Dystrophy Type 1, Marina Provenzano

Theses and Dissertations

Myotonic dystrophy type 1 (DM1) is a slowly progressive, multisystem disorder caused by a CTG repeat expansion in the DMPK 3’UTR that leads to global dysregulation of alternative splicing. The resulting decline in physical function is slow, and no reliable biomarkers exist for predicting disease progression; however, an RNA mis-splicing biomarker associated with weakness may have utility in predicting functional outcomes. Here we validate the Splice Index (SI) as a potential biomarker of DM1-associated strength and function. Muscle biopsies of the tibialis anterior were collected from DM1-affected individuals at baseline (n = 46) and 3-months (n = 34), along with …

Spatiotemporal Patterns For Agroforestry Tree Crops In The U.S. Corn Belt For Usda Census Of Agriculture Periods 2012–2022, Andria Caruthers, Justin Dijak, Robin M. Rotman

Faculty Publications

Within the U.S., there is a growing need to integrate environmentally friendly practices into conventional agriculture. Agroforestry enhances environmental and resource stewardship in agricultural landscapes while offering potential economic benefits to farmers. Despite rising interest, limited information on its application in the U.S. hinders development efforts. A spatiotemporal analysis of current farm operations can provide crucial insights. This study examined patterns of agroforestry and tree crop adoption in the U.S. Corn Belt using USDA Census data (2012, 2017, and 2022) and spatial tools (Global Moran’s I, Local Moran’s I, and Moran scatterplots). The tree crops included in the analysis were …

Antenatal Depression And Its Associated Factors: Findings From Kuwait Birth Cohort Study, Reem Al-Sabah, Abdullah Al-Taiar, Ali H. Ziyab, Saeed Akhtar, Majeda S. Hammoud

Community & Environmental Health Faculty Publications

Background: Pregnant and postpartum women are at high risk of depression due to hormonal and biological changes. Antenatal depression is understudied compared to postpartum depression and its predictors remain highly controversial.

Aim: To estimate the prevalence of depressive symptoms during pregnancy and investigate factors associated with this condition including vitamin D, folate and Vitamin B₁₂ among participants in the Kuwait Birth Study.

Methods: Data collection occurred as part of the Kuwait Birth Cohort Study in which pregnant women were recruited in the second and third trimester during antenatal care visits. Data on antenatal depression were collected using the Edinburgh …

How Financial Status Affects Food Decisions In Households Of Marin City, Joe Blakely

Global Public Health | Senior Theses

This research addresses disparities in food decision-making among households in Marin City by examining their food spending habits after covering essential bills. Understanding how these households allocate resources for food is crucial for developing targeted interventions to improve food security and health outcomes. Utilizing qualitative methods, semi-structured interviews, this study explores the spending patterns and decision-making processes related to food expenditures among households in Marin City. The sampling method ensures consideration of different economic levels and grocery strategies. Recruitment involves collaboration with community organizations and social service agencies such as Marin City Community Center. The study focuses on residents of …

Phylogenetic Relationships Of A North American Radiation Of Clematis (Ranunculaceae), Jesse Harris, Thomas H. Murphy, Erin A. Manzitto-Tripp, Nicolás Medina, Zach Irick, L. Dwayne Estes, Stacey D. Smith

Aliso: A Journal of Systematic and Floristic Botany

Clematis L. is one of many plant genera that contributes prominently to the species richness and endemism of the southeastern United States. Here we examined phylogenetic relationships among species in Clematis sect. Viorna, commonly known as leatherflowers, which are distributed primarily in the southeastern United States. We used data from restriction site-associated ddRADseq to reconstruct a best estimate phylogeny of this group. Our analyses revealed that the North American members of sect. Viorna form a well-supported clade, and several of the traditionally recognized infrasectional taxa were resolved as monophyletic. Although characters employed in classification including habit and inflorescence position …

Development And Evaluation Of A Retrofittable Variable Rate Applicator Controller, Ahmed Abdalla

Electronic Theses and Dissertations

The need to optimize resource utilization while increasing crop productivity is paramount. This research proposes a Low-cost Retrofittable Variable Rate Applicator Controller (VRAC) that aims to leverage the adoption of Variable Rate Technologies. The controller operates via the Raspberry Pi platform, a Global Positioning System (GPS), a stepper motor drive, and an anti-slip wheel encoder. The VRAC allows precise, on-the-fly control of variable-rate application of farming inputs. It utilizes GPS with 10 cm accuracy to pinpoint geographic coordinates in real-time. A wheel encoder measures traveling speed with a slip-resistant wheel design for precise RPM readings. The Raspberry processes the data, …

Kidney-Targeted Mrna Nanoparticles: Exploration Of The Mrna Loading Capacity Of A Polymeric Mesoscale Platform Employing Various Classes Of Excipients, Arantxa Roach

Biochemistry and Molecular Biology

Mesoscale nanoparticles (MNPs) for the therapeutic relief of renal ailments can be modulated to deliver various payloads – including biomolecules such as mRNA. In preparing mRNA loaded-MNPs, we observed a point of saturation for mRNA loading of these particles, when aiming to increase the payload per particle. Here, we aimed to circumvent this limitation by incorporating various excipients that interact with mRNA for increased loading. These interactions involved the reduction of mRNA electrostatic repulsion and improving mRNA stability during formulation and release. Thereafter, we tested the encapsulation efficiency of these modified particles and compared it to our original formulation. Further, …

Competition Between Bradyrhizobium Strains For Soybean Nodulation, Lilia Ernestina Montanez Hernandez

Electronic Theses and Dissertations

Biological Nitrogen Fixation (BNF) has surged as a natural strategy to provide available nitrogen to crops by taking advantage of diazotrophs, microorganisms that can reduce atmospheric nitrogen to ammonia. Soybean, a major crop, greatly benefits from BNF by forming endosymbiotic relationships with rhizobacteria from the genus Bradyrhizobium, which culminates in the formation of specialized organs called nodules, where nitrogen fixation occurs. This is known as Symbiotic Nitrogen Fixation (SNF). Inoculation of soybean fields with high nitrogen fixing Bradyrhizobium strains to stimulate SNF is being used as a strategy to increase crop productivity and nitrogen content. However, in North America, SNF …

The Behemoth Of The Last Ice Age: The Evolutionary Saga Of The Columbian Mammoth In North America, Nathaniel Valentine

Calvert Undergraduate Research Awards

The Columbian’s mammoth evolutionary history can be traced back to the origin of life and its evolutionary lineage can be tracked down by examining select species over geologic time. I conducted a literature review with multiple scientific journals to identify key transitional species that trace their lineage to the Columbian mammoth. Transitional species were selected from the Precambrian and the various periods of the Phanerozoic which exhibited vertebrate-like body structures and behaviors. Evidence suggests that early vertebrate-like animals were present by the Cambrian period. Mammal evolution would continue in the Mesozoic era and radiate in the Cenozoic era. The Columbian …

Variation In Las Vegas Tree Diversity Based On Socioeconomic Status, Fabrizio Ciccone

Calvert Undergraduate Research Awards

An increased level of plant biodiversity has been observed in urban communities of above average socioeconomic status. This phenomenon was previously termed the luxury effect. This study aimed to identify potential disparities in tree diversity of different communities across Las Vegas and Henderson. Communities were selected based on the zip code’s median income for 2023; this information was gathered from the Healthy Southern Nevada database. Public parks were chosen from a range of zip codes, with trees initially being identified with PictureThis, then cross-referenced with Southern Nevada Water Authority’s regional plant list for validity. Trees were counted, identified, and …

Unlv Title Iii Aanapisi & Mcnair Scholars Institute Research Journal 2024, Jesica Godinez-Paredes, Kian Hassankhan, Apia Hickman, Robin Ruth Kee, Kevin Ayala Pineda, Briana Melendez, Kalli Ramos, Saturn L. Reyes, Fabian Leija, Selena Pepe, Silva Topchyan, Celeste Ainsley, Mayra Arzate, Jessica Balistreri, Zantana Ephrem, Mirella Jasso, Karl Panou, Nichole Pelaez, Miklo Alcala, Akshay Dave, Mey Mey Heng, Michael Finkle, Janessa Montenegro, Reynafe Naol Aniga, Victor Mejia, Angelica G. Diaz, Anayeli Flores-Garibay, Kari Lee Joe Goold, Samantha Hernandez, Lianelys Cabrera Martinez, Caitlin Reynolds, Kimberly N. Usbeck, Abdulrahman Alahdal, Angelica Diaz, Willaine Mae Kahano, Raquel Jackson, Cecia Ruiz-Hernandez, Kers Ung-Watson, Yessenia Henriquez, Vanessa Marie Booth, Alexia Brown, Darlyn Magana, Bianca Navarro, Nicholas Pereira, Gia Renemae Calip, Lucky Heng, Ralph Sagun, Lucas Abreu, Alexandra Maria Acosta, Tristan Benally, Cosset Hernandez Pena, Adrian Montenegro, Cierra Paaaina-Daquioag, Nicole Torosian, Tracy Fuentes, Julissa Martinez, Medina Mcallister, Michal Newhouse-Van Vlerin, Tiria Carr, Nima Abkenar, Isabella Aceituno, Yuhan Bi, Victoria Campos, Melika Cummings, Zachary J. Johnigan, Alexis Sotolongo-Marin

McNair Journal

Journal articles based on research conducted by undergraduate students in the AANAPISI, LSAMP, and McNair Scholars Program.

Table of Contents

About AANAPISI

Dr. Chris Heavey, Interim President

Dr. Keith Rogers, Vice President for Student Affairs

Ms. Zhanna Aronov, Associate Vice President for Retention & Outreach

The Choice Is Hers: Floral Preference And Habitat Use Of Early Season Queen Bumble Bees In Restored Ecosystems, Kathryn E. Mccabe

Graduate Research Theses & Dissertations

Restored ecosystems play a vital role in providing a sanctuary for native flora and fauna, offering crucial habitat and resources for many at-risk and declining taxa. Ensuring optimal functionality of these restorations is essential to supporting taxon like bumble bees. While many studies have focused on understanding the needs of bumble bees, much of this effort has relied on information gathered during their peak foraging season when workers are abundant, thus, neglecting other critical life stages, such as emerging spring queens. In this study, I investigated how spring queen bumble bees use three types of restored habitats (woodland, edge, and …

Genetic Engineering Of Escherichia Coli For The Production Of Riboflavin, Adeyemi Adesanwo

Graduate Research Theses & Dissertations

Riboflavin, a water-soluble vitamin B2, is essential for the growth and reproduction of living organisms. However, humans and animals lack a riboflavin synthesis pathway and must rely on diets derived from plants and microbes for this essential vitamin. To meet our needs for vitamin B2, industrial riboflavin production is currently being pursued using strains of Bacillus subtilis. The fermentation process is successful, but improvements are needed for better substrate-to-product conversion yield. To this end, it is imperative to develop alternative microbial strains for cost-effective riboflavin production.

Due to its simple growth requirement, well-understood genetic background, and widely available genetic tools, …

Roles Of Ppp1r15a (Gadd34) And Ppp1r15b (Crep) In Er Stress-Induced Apoptosis In Zebrafish Caudal Fin Epidermal Cells, Ayano Ohata

University of the Pacific Theses and Dissertations

ER stress occurs in response to the accumulation of unfolded or misfolded proteins in the ER lumen, subsequently activating three signal transduction pathways collectively called the unfolded protein response (UPR). Although the goal of the UPR is to restore ER homeostasis, it can result in apoptosis when ER stress is too severe or prolonged. CHOP, which is induced by all three branches of the UPR, is central to inducing ER stress-induced apoptosis by regulating the expression of numerous pro-apoptotic genes. One of the downstream targets of CHOP is GADD34, which contributes to PP1-mediated dephosphorylation of eIF2α together with its homolog …

Volume 15, Connor Thompson, Emily Steffenhagen, Emily Robertson, Luis Fernando Dos Reis, Emily Farmer, Samuel Villa, Robert Allison, Zachary Chessor, Megan Borden, Austin Burnett, Larry W. Grant Jr., Tristan Marowski, Emma Moore, Pearl Siff

Incite: The Journal of Undergraduate Scholarship

Introduction Dr. Amorette Barber, Director, Office of Student Research

From the Editor Dr. Hannah Dudley-Shotwell

Artist’s Statement Connor Thompson

On Mentorship Dr. John Miller

The Meat of the Matter: Alien, Human, and Animal in Terry Bisson’s “They’re Made Out of Meat” by Emily Steffenhagen

“Please REBLOG!”: An Ethical Analysis of Doxxing, Internet Vigilantism and Racists Getting Fired by Emily Robertson

Journaling: Paper Has More Patience Than People by Luis Fernando Dos Reis

The Effects of Climate Change on the Archaeological World by Emily Farmer

Lowered Seat Height Does Not Impair Wingate Performance in Untrained Cyclists by Samuel Villa, Robert Allison, …

Diving At High Altitude: O2 Transport And Utilization In The Ruddy Duck And Torrent Duck In The Andes, Kevin G. Mccracken, Graham R. Scott, Luis Alza, Andrea Astie, Ciska Bakkeren, Emil Bautista, Mariana Bulgarella, Rebecca Cheek G. Cheek, Beverly A. Chua, Neal J. Dawson, Alexis Diaz, Catherine M. Ivy, Peter B. Frappell, Cecilia Kopuchian, Sabine L. Laguë, John N. Maina, Violeta Muñoz-Fuentes, Elizabeth R. Schell, Matthew M. Smith, Ryan J. Sprenger, Pablo L. Tubaro, Elizabeth R. Schell, Matthew M. Smith, Ryan J. Sprenger, Pablo L. Tubaro, Thomas Valqui, Roy E. Weber, Daniela Wilner, Robert E. Wilson, Julia M. York, William K. Milsom

Occasional Papers of the Museum of Natural Science, Louisiana State University

Hypoxia and cold temperatures create unique physiological challenges for high-altitude organisms that can vary depending on lifestyle. While nearly all studies of air-breathing animals at high altitude are from terrestrial species, species that breath-hold dive underwater at high altitude encounter a very different set of selective pressures influencing their phenotype. The goal of this publication is to highlight the changes in O2 transport and utilization in high-altitude diving birds relative to divers at sea level, and the extent to which these changes are qualitatively distinct from phenotypic changes in non-diving species at high altitude. For example, while high capacities for …

Louisiana Agriculture Magazine, Winter 2024, Lsu Agcenter

Louisiana Agriculture

Research at the LSU AgCenter extends well beyond the farm gate. Across 150 acres at the Hammond Research Station, experts in landscape horticulture test the best ways to grow ornamental plants and trees. In Baton Rouge, researchers study how to protect homes in the path of a hurricane. Across the state the AgCenter Healthy Communities team works to empower food pantries and improve sidewalks and other pedestrian infrastructure in rural areas.

This issue of Louisiana Agriculture focuses on these topics along with new research on soil health and high-tech delivery systems for sugarcane ripening agents. Other articles focus on the …

Nitrogen Application Timing And Cereal Rye (Secale Cereale L.) Cover Crop Influence Greenhouse Gas Emissions, Graig Reicks

Electronic Theses and Dissertations

Dryland contributions to greenhouse gas (GHG) emissions are increased by N fertilizer applications and high soil water contents. Fertilizer timing and spring growth of cover crops prior to cash crop planting were investigated in separate studies to examine impacts on overall GHG emissions using a near continuous measurement system. There was a significant interaction between N fertilizer rate (0 vs. 224 kg N ha-1 surface-applied as urea) and application date (p= 0.01) for CO2 emissions. This interaction occurred because N fertilizer increased CO2 emissions by 35% for the 21 d interval following early spring application. When application was delayed until …

Primary Cilia And Intraflagellar Transport Are Essential Regulators Of Lymphangiogenesis And Modulate Cellular Trafficking Of Ve-Cadherin, Delayna Paulson

Electronic Theses and Dissertations

No abstract provided.

Physicochemical And Functional Properties Of Different Varieties Of Spring And Winter Peas Of South Dakota, Prakriti Dhakal

Electronic Theses and Dissertations

Peas are a good source of nutrients for both humans and animals, and they are the world's second most significant and sustainable legume. There have been studies on different kinds of pea grown in other parts of the world, but there is no information on the compositional and functional characteristics of pea varieties grown specifically in South Dakota. The objective of the study was to evaluate the proximate composition and functional properties of various pea varieties and determine if the pea variety impacted cookie properties. The proximate composition (moisture, protein, starch, and amylose), functional capacities (water holding, oil holding capacity, …

Developing Machine Learning Models For Selection Of Management Zones, Sravanthi Bachina

Electronic Theses and Dissertations

Soil sampling and analyses play a crucial role in optimizing nutrient management and enhancing crop productivity. However, collecting representative samples across diverse landscapes is challenging due to knowledge gaps about spatial variability of soil properties, large fields, multiple samples, and analysis costs. Collecting soil samples based on the management zones can help farmers gather precise information about soil properties with fewer samples. Recent developments in precision agriculture and machine learning. This study aimed to develop machine learning models that can learn, analyze, and refine landscape and soil properties data for automated selection of soil sampling zones and generating prediction maps. …

Species Distribution Modeling Of Aquilegia Brevistyla (Ranunculaceae): A Critically Imperiled Black Hills Disjunct Species, Eric Daniel Puetz

Electronic Theses and Dissertations

Unchecked human activity is contributing to rising levels of greenhouse gas emissions, changes in land use, altered disturbance/fire regimes, spread of invasive species, and loss of biological diversity and related breakdown of ecosystem services. Additionally, climatic shifts may lead to phenological mismatches between species and their environments if these changes outpace a species’ ability to adapt or migrate to a more suitable habitat. Isolated mountain populations are particularly threatened by unpredictable climatic conditions, as they may have limited migration corridors and often lower levels of genetic diversity to move or adapt, respectively. As these negative feedbacks compound on the landscape, …

The Hutton Project: Long-Term Agricultural Impacts On Soil Loss And Carbon Dynamics In Eastern South Dakota, Eli Halverson

Electronic Theses and Dissertations

Long-term and intensified agricultural land management has resulted in increased rates of soil erosion and has altered much of the carbon cycle at regional and global scales. Anthropogenic degradation of soil resources is a barrier to sustainable production, soil functioning, and ecosystem services. It is difficult to quantify the scope of pedogenic changes due to the lack of legacy data and short temporal scales. This study utilized decades to century-old soil information to quantify historical soil erosion losses and changes in soil carbon pools of eastern South Dakota soils. The results show that soils in the region have been significantly …

Plant Pathways Associated With Competition For Soybean Root Colonization By Bradyrhizobium Strains, Athira Sethu Madhavan

Electronic Theses and Dissertations

Nitrogen is a crucial nutrient for the growth and development of plants. Legume crops like soybeans develop symbiotic associations with soil bacteria from the genus, Bradyrhizobium to obtain fixed nitrogen. In this mutualistic association, rhizobia are released into root nodules that provide a compatible environment for rhizobia to grow and fix nitrogen (Symbiotic Nitrogen Fixation (SNF)). A caveat in such beneficial associations is the competition between different rhizobium strains in soil for infection and nodule occupancy. The elite inoculant strains encounter competition by indigenous rhizobial population in the soil to infect and nodulate a susceptible legume host, because of which …

Feeding Isoacids To Swine: Inclusion Level And Interactions With Dietary Fiber And Protein, Angie Patricia Benavides Infante

Electronic Theses and Dissertations

In animal production, research is continually being carried out into implementing new feeding strategies, such as incorporating new alternative ingredients or additives that improve the quality of the diet, reduce the cost of diets, or have effects on intestinal health. The inclusion of branched-chain volatile fatty acids has been investigated in ruminants, and it has been found that a diet supplemented with BCVFA increases fiber digestibility as well as increases cellulolytic bacteria in the rumen. There is little information about the possible effects on pigs supplemented with BCVFA. According to this, the first study aims to evaluate the optimal inclusion …

Iron–Sulfur Cluster Assembly Scaffold Protein Iscu Is Required For Activation Of Ferric Uptake Regulator (Fur) In Escherichia Coli, Huangen Ding

Faculty Publications

No abstract provided.

"It Is More Than A Feeling, It Is A Commitment" : Evaluation Of Empathy Development Using Graphic Novels In A Medical Humanities First-Year Seminar, Susanna Foxworthy Scott, Brittany R. Heer, Alli Leonard

Scholarship and Professional Work

No abstract provided.

Forensic Archaeology Protocols For Wildfires And Fire Related Scenes, Erik Schulz

Nebraska Anthropologist

Forensic Archaeology Protocols for Wildfires and Fire-Related Scenes

Forensic archaeology is a relatively new area of study and focuses on the medical legal aspects of archaeology. This paper will focus on how forensic archaeology should be used for wildfire and other fire-related fatalities or investigations, what happens to bone when exposed to heat, what protocols should be in place and how an excavation should look, and finally the challenges of fire-related investigations. This report will focus on the larger scale of the investigation and will be using several sources from different wildfire and fire fatality reports.

Effects Of Low-Cost, Low-Tech Tools For Riparian Restoration On Prairie Streams In Western South Dakota, James Andrew Joseph Bolyard

Electronic Theses and Dissertations

Despite making up less than two percent of the overall landscape in the arid and semi-arid western US, riparian areas are a crucial resource for agriculture, livestock, and wildlife. However, many have impaired function and reduced riparian cover. Low-Cost, Low-Tech Tools (LCLTT) are a subset of Process-Based Restoration (PBR) used for riparian restoration that were chosen for their cost-effectiveness and minimal technical requirements. LCLTT has been tested in mountainous areas of the western US but only recently implemented in the Northern Great Plains (NGP). Given their novelty as an approach toward restoration for the region, professional restoration and landowner communities …

Near-Real-Time Monitoring Of Crop Progress At Field Scales By Fusing Observations From Both Polar-Orbiting And Geostationary Satellites, Yu Shen

Electronic Theses and Dissertations

No abstract provided.