Life Sciences Commons^™

Impact Of Inhibiting Mitochondrial Calcium Uptake On Calcium Homeostasis And Postmortem Metabolism In Porcine Muscle, Fatema Rahaman Mishu

All Graduate Theses and Dissertations, Fall 2023 to Present

After harvesting the animal, pork muscle continues to undergo changes as it turns from muscle into meat. During this early period, the muscle uses up energy and produces acid, which lowers pH and can affect important quality traits such as color and how well the meat holds water. A key driver of these early changes is calcium, a natural mineral inside muscle cells that helps control contraction and energy use. In this study, we tested whether mitochondria (the cell’s “powerhouse”) help control calcium after harvest and, in turn, influence early postmortem changes in pork. We used a research compound (DS16570511) …

Influence Of Predation Risk On Elk Feeding Behavior In A Multi-Predator Environment, Rosemarie C. Pugh

All Graduate Theses and Dissertations, Fall 2023 to Present

Sometimes predators don’t just affect prey by killing them, they can also change how prey behave. For example, animals may spend more time watching for danger and less time eating, which could affect their health and survival. In Yellowstone National Park, past research has suggested that elk may reduce feeding because they are trying to avoid predators like wolves and cougars. However, most previous studies have only looked at elk behavior during the daytime and have often looked at responses from single predators rather than multiple.

In this study, I used high-resolution tracking collars equipped with motion sensors to directly …

Effects Of Mineral Supplementation On Ovine Performance, Forage Selectivity, And Rangeland Utilization In Southern Utah, Ryan A. Spurling

All Graduate Theses and Dissertations, Fall 2023 to Present

Sheep producers in the Intermountain West region of the US rely on productive rangeland ecosystems that vary widely in forage composition and nutrient availability. As such, supplementation is often necessary to offset dietary deficiencies. Soils and forages grown on rangelands are known to be highly variable in mineral content. Even so, a third to half of the producers in the Intermountain West choose not to provide a complete mineral supplement. Rangeland environments are often characterized by rugged geographical features that make optimal supplement placement difficult. New advances in technology have allowed us to measure individual animal intake on rangelands via …

Alfalfa Yield, Quality, And Nutrient Use Response To Pivot Sprinkler Technology, Deficit Irrigation, And Genetics, Dakota Boren

All Graduate Theses and Dissertations, Fall 2023 to Present

In water limited environments, alfalfa is often criticized for its high water use, prompting implementation of new irrigation technologies, deficit irrigation, and adapted crop genetics. Cumulative potential benefits from combining, or stacking, these water-optimizing practices have not been identified. The objective of this on-farm research study was to determine how irrigation technologies, deficit irrigation, genetics, and combinations of these practices impact alfalfa yield and forage quality. Trials were conducted at three sites in Utah from 2020 to 2022 on young alfalfa stands (establishment and first one to three production years). Treatments included five irrigation technologies, four irrigation rates, and two …

Effect Of Agroecological Feeding Systems Of Dairy Cattle On The Nutritional Composition Of Milk, Sree Bhavya Immadi

All Graduate Theses and Dissertations, Fall 2023 to Present

How food is produced can influence both its nutritional quality and its impact on the environment. In recent years, regenerative (agroecological) farming practices have gained attention for their potential to improve soil health, biodiversity, and the nutritional value of foods. In dairy production, these practices often involve growing more diverse forage crops, reducing soil disturbance, and relying less on monoculture-grown feed rations. However, there is still limited real-world evidence showing how these practices affect the nutritional composition of milk produced on commercial dairy farms.

This dissertation explores how regenerative feeding and feed production practices influence the nutritional quality of milk, …

Effects Of Serum And Commercial Serum-Free Media On Development Of In Vitro Produced (Ivp) Embryos In Cattle, Kyla Miller

All Graduate Theses and Dissertations, Fall 2023 to Present

Serum-containing media has been shown to increase incidence of large offspring syndrome and to alter gene expression, protein synthesis, and cell signaling. These factors together can result in increased pregnancy loss when combined with assisted reproduction technologies. However, there are very few direct comparisons of commercial serum-free media (IVF Bioscience™) and serum-containing media on both IVF and cloned embryo development in cattle. The aim of this study was to determine the role of serum in oocyte maturation and subsequent embryo development. Three culture systems, including in vitro maturation (IVM) and in vitro culture (IVC), were used in this study. Both …

Exploring The Correlation Between Malnutrition And Food And Nutrition Insecurity In Inflammatory Bowel Disease, Heather Burr

All Graduate Theses and Dissertations, Fall 2023 to Present

Inflammatory Bowel Disease (IBD) may increase the risk of malnutrition because of nausea, vomiting, diarrhea and restrictive eating. In addition, patients with IBD may experience food and nutrition insecurity. Therefore, we conducted an online survey to assess risk of malnutrition and food and nutrition insecurity among adult patients with IBD in outpatient and community settings. Patients were recruited between July and December 2025, aiming to reach adults in the Rocky Mountain Region diagnosed with IBD. Data collected included demographics, health-related factors, risk of nutrition insecurity, risk of food insecurity, and barriers and facilitators to dietary management of IBD.

Fifty-seven patients …

Evaluating Short-Day And Intermediate-Day Sweet Onions As Early-Season High Tunnel Onions In West Virginia, Lewis Jett

Midwest Vegetable Trial Reports

High tunnels can be used to grow winter alliums such as onions, garlic and leeks.  Onions are  diverse and include groups such as short-day and intermediate-day bulb onions as well as non-bulbing scallions or green onions.  Onions can be diverse in color, size and shape.  Short day onions are typically sweet onions which start bulbing when photoperiods reach 10-12 hours. Short-day onions are typically planted in November-December in the Deep South of the U.S. for spring harvest.   Intermediate day onions start bulbing when the photoperiod is 12-14 hours.  Intermediate day onions are planted in the spring in the Mid-Atlantic region. …

Developing Training And Streamlining Training Management Process For Smithfield Fire Department Employees In Aha, Naemt, And Aap Certifications, Michael Christopher Popa Ii

Undergraduate Honors Capstone Projects

The rapid operational expansion of the Smithfield Fire Department and EMS (SFD)—marked by a 300% increase in call volume and a transition to a fully professional, independent paramedic service—exposed critical systemic inefficiencies in clinical preparedness and personnel credentialing. This project addresses the "HALO Paradox," wherein high-acuity, low-occurrence emergencies require expert-level proficiency that cannot be maintained through call volume alone. Historically, the agency relied on fragmented, external continuing education (CE) that incurred high financial costs, lacked equipment standardization, and created administrative bottlenecks during biannual recertification cycles. The central objective of this capstone was to engineer a sustainable, in-house training infrastructure that …

The Hitchhiker's Guide To The Host: Fleas And The History Of Pikas In North America, Josh Pletcher

All NMU Master's Theses

Parasite phylogenetics can offer windows into the cryptic evolutionary history of hosts, often highlighting events that are not recorded by the phylogenetics of the host. Pikas (small lagomorphs) host a diverse parasite community comprising endoparasites (e.g., coccidians, nematodes, and cestodes) and ectoparasites (e.g., fleas and mites). Phylogenetic analysis of pika endoparasites has revealed pikas evolved in Asia and colonized North America in two waves, and that contact between these two pika lineages allowed parasites to be transferred from one lineage to another and to survive the extinction of their original host. To uncover these cryptic histories, understanding the biogeography of …

Cell-Type Specific Control Of Visual Fixation In Drosophila Melanogaster, Meagan Leung

Biological Sciences

Navigation in complex environments requires precise visual processing. To move effectively through their surroundings, animals must orient toward and maintain fixation on objects of interest to preserve a stable heading. However, the neural mechanisms underlying this behavior remain poorly understood. We use Drosophila melanogaster as our model organism because its visual behavior can be precisely measured and the availability of genetic tools which enables us to perturb the underlying neural circuits. Using a closed-loop virtual reality environment combined with locomotion tracking, we developed a stimulus protocol that reliably elicits fixation responses. To determine whether specific visual neurons drive fixation responses, …

Long-Term Stimulant Use Is Associated With Normalized White Matter Integrity In The Vta-Nacc Pathway In Adolescents, Isaac T. Arsenault

Biological Sciences Undergraduate Honors Theses

Medical practitioners are diagnosing Attention-Deficit/Hyperactivity Disorder (ADHD) at a high and ever-increasing rate. The use of stimulant medication to treat ADHD is also increasing and is associated with changes in the brain. Past research found that children with ADHD have lower volume in reward neurocircuitry, such as the ventral tegmental area (VTA) and the nucleus accumbens (NAcc). Research has also found that stimulant usage in those with ADHD might “normalize” the reward circuits. However, few longitudinal studies have directly tested the long-term effects of stimulant medication on brain development. Here we tested whether long-term stimulant use is associated with changes …

Dfend: A Nutrition Education Intervention To Improve Food Budgeting, Shopping, And Meal Preparation Skills Among Adults In Arkansas, Samantha K. Edwards

Human Nutrition and Hospitality Management Undergraduate Honors Theses

Preventive strategies targeting modifiable lifestyle factors related to food, nutrition, and health are needed as chronic health conditions continue to rise. Nutrition literacy refers to having the knowledge and skills needed to obtain, understand, and apply nutrition information to make informed dietary decisions. Without adequate nutrition education and food security, vulnerable populations are at risk of malnutrition and poor dietary behaviors. Educational interventions that increase food literacy can help improve dietary choices and food selection skills.

The purpose of this creative project was to develop a nutrition education toolkit for Extension agents to implement among rural populations with low health …

Leveraging Historic Collections: Investigating Morphometric Differentiation In Members Of The Arisaema Triphyllum (L.) Schott (Araceae) Species Complex, Morgan Bradley Russell

Graduate Theses and Dissertations

The Jack-in-the-pulpit (Arisaema triphyllum (L.) Schott) is an herbaceous perennial herb of North America, and its taxonomic history is full of confusion and rank shifts. Now split into six species, the A. triphyllum species complex consists of morphologically similar taxa that have differing ploidy levels. In Chapter I, I compiled a detailed introduction and history for these taxa, focusing on their taxonomic complexity and morphological ambiguity in herbarium specimens and immature plants. I also presented their ploidy distinctions and how the implications of polyploidy may hold answers to parsing them apart, especially in dried herbarium specimens where defining characteristics are …

Impact Of Salt Block Utilization During Dry-Aging On Pork Loin Microbial And Meat Quality, Cason H. Frisby

Food Science Undergraduate Honors Theses

This study aimed to investigate the effects of dry-aging and salt block utilization during dry aging on pork loin quality attributes. Pork loins were collected at 7 days postmortem, cut into three equal sections, and randomly assigned to wet aging (WA), traditional dry-aging (DA), and salt block dry-aging (SDA) groups to be aged for 28 days. During the dry-aging period, SDA cabinet exhibited lower humidity fluctuation (Avg. 71.52, St. Dev. 4.7) compared to DA cabinet (Avg 70.76, St. Dev. 7.9). Following the aging period, no differences were found in pH or Warner Bratzler shear force values between treatments (P>0.05). …

Precision To Protein: Comparative Characterization Of Printability, Physicochemical, And Nutritional Properties Of Cricket, Pea, And Whey Proteins In 3d-Printed Nutrition Bars, Sophia Hinkebein

Food Science Undergraduate Honors Theses

Protein is a priority nutrient in the American diet, being fueled by the demands of weight loss drugs, diet culture, and new dietary guidelines. However, meeting these elevated protein goals sustainably requires diversifying beyond standard sources like whey protein isolate. 3D printing offers an opportunity to present a variety of protein sources in a palatable, customized, and consumer-acceptable format. In this study, 3 protein sources (cricket, whey, and pea), were optimized within food inks for 3D printing. Optimization involved different hydration levels, with whey requiring 7 grams of added water, cricket 22 grams, and pea the most at 47 grams. …

Mammalian Biodiversity Of The Easley Conservation Stewardship Area, Grayson C. Belt

Honors College Theses

Monitoring wildlife is an important part of conservation and is particularly vital for newly acquired conservation areas to provide a baseline of what is present. In 2025, the 22 ha Murray State University Easley Conservation Stewardship Area (MSU ECSA) was established following a donation to the university by Melissa Easley. I conducted a survey using trail cameras at the ECSA in fall 2025 and winter 2025/26 to record mammalian diversity. The survey consisted of two subprojects: a mesomammal subproject of 18 knee height trail cams and a small mammal subproject using 15 custom small mammal bucket cameras. I uploaded all …

Population Structure And Connectivity Of Atlantic Nurse Sharks (Ginglymostoma Cirratum) In The Bahamas And Outlying Areas, Baylie A. Fadool

School of Natural Resources: Dissertations, Theses, and Student Research

Understanding animal ecosystem dynamics and behavior at the individual and population levels is essential for identifying life-history patterns and important areas for management and conservation. Marine fish species often face minimal geographic barriers to movement, but environmental (temperature, salinity, current) or intrinsic barriers (philopatry, site fidelity) can dictate gene flow. Atlantic nurse sharks (Ginglymostoma cirratum) are an International Union for Conservation of Nature (IUCN) listed ‘vulnerable’ species and present a unique opportunity to investigate patterns of population structure and connectivity in large-bodied sharks. They are benthic and display reproductive philopatry, with recent work suggesting they have the capacity …

Exploring Student Sensemaking During A Collaborative Biology Lab, Rebecca Kollipara

School of Natural Resources: Dissertations, Theses, and Student Research

Collaborative learning environments offer valuable opportunities for students to construct scientific understanding through dialogue, yet the conditions that support sustained conceptual reasoning in group settings remain incompletely understood. While prior research has identified discourse moves associated with productive collaboration, less attention has been paid to how those moves function together across turns and conceptual boundaries, or how individual student characteristics shape their emergence. This thesis examines collaborative sensemaking in an undergraduate General Biology II course, drawing on two complementary analyses of student discourse during a bryophyte life-cycle modeling activity.

Chapter 1 investigates how student groups participated in sensemaking and how …

Gaining Mussel: Evaluating Reintroduction Success Of Native Freshwater Mussels In Nebraska And Eastern Wyoming, Travis A. Moore

School of Natural Resources: Dissertations, Theses, and Student Research

Freshwater mussels are one of the most imperiled groups of organisms in North America, with over two-thirds of species considered threatened, endangered, or of special concern. These long-lived bivalves are essential to stream ecosystems, acting as filter feeders that improve water quality and as indicators of aquatic health. In Nebraska and Wyoming, native mussel populations have declined sharply due to habitat modification, pollution, and reduced connectivity. Two species of particular concern are the Plain Pocketbook Lampsilis cardium, listed as Tier I at-risk in Nebraska, and the Fatmucket Lampsilis siliquoidea, a Tier II at-risk species. In response, state agencies …

The Impact Of Glucose Exposure On Organic Cation Transporters (Oct) Function In A Corneal Epithelial Cell Line, David A. Garza

Theses & Dissertations

Organic cation transporters (OCT) facilitate the movement of neurochemicals and medications, contribute to the removal of cellular waste, and facilitate neuronal communication. Three subtypes of these high-capacity, low-affinity transporters (OCT1, OCT2, and OCT3) are differently expressed in peripheral tissues such as the liver, kidneys, as well as the central nervous system. OCTs are also expressed in ocular tissues. Chronic hyperglycemia is a complication commonly associated with diabetes that can lead to ocular diseases such as diabetic keratopathy, which affects the cornea. In this project, we expose immortalized human corneal epithelial cells (HCE-S) to various glucose concentrations to study their impact …

Honeybee Colony Response To Probiotic Supplementation And Grazing Practices, Katya M. Busenitz

Department of Entomology: Dissertations, Theses, and Student Research

Honeybee health is a complicated issue. Studies were conducted to scale honeybee (Apis mellifera) health from the microbiome to the landscape. Colony health parameters were measured in response to probiotic administration. Colony capped brood production, colony summer weight gain, and Varroa mite incidence were measured. I found that applications of over-the-counter probiotics did not improve the measured parameters of honeybee health compared to a control group. Results suggest that probiotic application was not beneficial but positive benefits may depend on application method, brand, or strain of probiotics used. I also examined how colony health metrics responded to different …

Bio 23 Human Anatomy & Physiology I Syllabus, Rujin Tian

Open Educational Resources

The syllabus for BIO 23 contains a course description, course objectives, course readings divided by week and a link to an OER textbook.

Bio 24 Human Anatomy And Physiology Ii, Rujin Tian

Open Educational Resources

The syllabus for BIO 24 contains a course description, course objectives, course readings divided by week and a link to an OER textbook.

Inosine Accelerates Cancer Cell Killing By The Experimental Antifolate Ucp1162: Implications For Novel Combination Treatments, Reshma Ramesh, Charles Giardina, Alan Kuo

Honors Scholar Theses

Antifolates disrupt one-carbon metabolism (OCM), which stops nucleotide synthesis and therefore slows down cell proliferation. Our experimental antifolate, UCP1162, stops de novo purine synthesis and thymidine synthesis by targeting the OCM pathway. We investigated whether the downstream metabolites of OCM pathway: methionine, thymidine, and inosine, affect the cell death caused by UCP1162 using MV4-11 leukemia cells. Thymidine was found to partially rescue MV4-11 cells from UCP1162, in contrast, inosine was found to accelerate the cell death caused by UCP1162. These cytotoxic effects of UCP1162 alone and in combination with inosine were irreversible, as removal of treatment did not restore cell …

Validation Of A Real-Time Pcr Laboratory Developed Test For The Detection Of Pneumocystis Jirovecii In Bronchoalveolar Lavage Samples, Caitlyn Moon

Honors Scholar Theses

Pneumocystis jirovecii is an opportunistic, host-obligate fungal pathogen responsible for causing P. jirovecii pneumonia (PJP) in humans. PJP is treated with trimethoprim-sulfamethoxazole (TMP-SMX), a strong broad-spectrum antibiotic that carries risk of renal and hepatotoxicity. Therefore, rapid diagnosis or exclusion of PJP is crucial to ensure PJP-positive patients receive effective treatment and PJP-negative patients avoid unnecessary antibiotics. Unlike most microorganisms, P. jirovecii cannot be cultured, presenting complications for the typical methods of diagnosing fungal pathogens. Historically, the gold standard for diagnosis was direct microscopic examination with stains such as methenamine silver and calcofluor white, but this diagnostic technique requires highly skilled …

The Development Of A Crispr-Based Calibrated Functional Assay For Classifying Pathogenicity Of Msh2 And Msh6 Variants In Lynch Syndrome, Olivia N. Amodeo

Honors Scholar Theses

Lynch syndrome is a hereditary disease caused by the inheritance of a mismatch repair gene variant. Individuals with this condition are predisposed to cancer development, most commonly colorectal cancer. Current guidelines for variant classification are based on numerous evidence categories, including functional evidence. However, many novel clinical variants are not well characterized, and evidence is difficult to obtain if functional assays are not calibrated.

To address this, our lab created a calibrated functional assay that calculates an odds of pathogenicity score for MSH2 and MSH6 gene variants that can be used as evidence for classifying variants of uncertain significance. This …

Reversible, Light-Controlled Inhibition Of Stim1-Mediated Calcium Signaling Using Photoswitchable Ml-9 Derivatives In Jurkat-Lucia Nfat Reporter T Cells, José L. Matute Gálvez

Honors Scholar Theses

Store-operated calcium entry (SOCE) through calcium release-activated

calcium (CRAC) channels is the dominant pathway for sustained Ca2+ influx in

non-excitable cells and is essential for T cell activation, driving downstream

signaling through the calcineurin-NFAT transcriptional axis. Dysregulation of

SOCE produces multi-systemic disease phenotypes, establishing that precise,

spatiotemporally controlled modulation of this pathway is a fundamental

therapeutic requirement. ML-9 is one of the only characterized small molecules

capable of targeting SOCE upstream at the level of STIM1 localization, but lacks

the selectivity, pharmacokinetic properties, and spatiotemporal control necessary

for therapeutic development.

This work describes the design, synthesis, and biological evaluation of …

Optimizing Citriculture In South Texas With Endemic Hlb, Ruth Lilia Galan

Theses and Dissertations

The Texas citrus industry has a long history of resilience, rebounding from environmental and economic disasters. Over the last decade it has endured several hurricanes, freezes, pests and diseases. Huanglongbing (HLB), a graft-transmissible disease caused by Gram-negative bacterium Candidatus Liberibacter asiaticus which is vectored by the Asian citrus psyllid (ACP) (Diaphorina citri Kuwayama), is an endemic citrus disease posing serious threat to the long-term viability of the industry. Rapid production of high quality nursery trees and effective protection of young citrus transplants from HLB are critical for long term sustainability of citriculture in this region. Studies were performed to improve …

Phenotypic Contribution And Regulation Of Vibrio Parahaemolyticus Cold-Shock Protein Homologue Vp1889, Oleg Borisovich Ermolinsky

Theses and Dissertations

Vibrio parahaemolyticus is the primary causative agent of a form of gastroenteritis called vibriosis commonly acquired from ingestion of raw and undercooked seafood. The primary mitigation of seafood-borne illnesses is immediate storage on ice following catch, which inhibits bacterial metabolism and replication. Mesophilic bacteria such as V. parahaemolyticus induce expression of cold-shock proteins in a process named the cold shock response, which facilitates survival at low temperatures. V. parahaemolyticus bears 3 homologues of the major Escherichia coli cold-shock protein CspA, an RNA chaperone. Induction of E. coli CspA at low temperatures depends on autogenous modulation by its mRNA structure, which …