Life Sciences Commons^™

Livestock Water Requirements And Water Budgeting For South West Western Australia, Department Of Primary Industries And Regional Development, Western Australia

Animal production and livestock factsheets

Adequate supplies of good quality stock water are just as important as feed supplies during summer, a period when feed and water quantity and quality are at their lowest in the agricultural areas of Western Australia.

Information on this page will help livestock owners to calculate a water budget – before problems develop – to complement a feed budget.

The Evaluation Of Compound X In A Mouse Model In Chikv Disease, Kaitlyn Blotter

Undergraduate Honors Capstone Projects

Chikungunya virus (CHIKV) is an infectious alphavirus spread by Aedes aegypti and Aedes alpopictus mosquitoes. Recently, there have been various outbreaks in the Americas, the Caribbean, and across Asia and Africa (Bettis et al., 2022). Although mortality rates of Chikungunya disease are relatively low, the disease has been shown to have high morbidity and chronic effects. Currently, there are no approved antivirals for treatment of CHIKV infection. The goal of this study was to identify whether Compound X is an effective treatment in a mouse model of CHIKV infection. Compound X was active in cell culture models of CHIKV infection, …

An Investigation Of Protein Arginine Methyltransferase 1 Substrate Recognition Through Ribonucleoprotein Modifications, Isabella Beverly Lonardo

Undergraduate Honors Capstone Projects

Just as humans need to communicate with each other, our cells have a series of processes that allows them to communicate from one to another. Protein Arginine Methyltransferase I is an enzyme that helps mediate cellular communication by selectively binding to target proteins and performing chemistry that has broad-reaching downstream effects. However, the mechanisms that underly PRMT1 selectivity have yet to be elucidated. This study uses alterations to a series of known targets, called ribonucleoproteins, to identify some of the characteristics that govern recognition by the PRMT1 enzyme. I have made significant progress in the creation, expression, and purification of …

Effects Of Humidity On Native And Non-Native Cavity Nesting Solitary Bees, Elleke Grace Kofford Manwill

Undergraduate Honors Capstone Projects

Invasive species are defined as those that are not native to an ecosystem but may cause harm to the environment or to human health. Osmia cornifrons, a species introduced to the United States, and Osmia lignaria, a native species, are cavity-nesting solitary bees. Although not officially classified as invasive, O. cornifrons presents competition for the niche that O. lignaria inhabits. O. cornifrons has established populations throughout the East Coast and the Pacific Northwest, while O. lignaria is found all throughout the contiguous United States. This interspecies difference in geographical range exists despite their similar temperature ranges. To determine …

How Variation In Activity Time And Duration At Water Sources Affects Feral Horse Vulnerability To Cougar Predation In Southern Nevada, Katelyn Davies

Undergraduate Honors Capstone Projects

Feral horses have become prominent in the Intermountain West. Approximately 43,000 horses are found in Nevada alone. With increasing numbers, they have become a large portion of ungulate biomass. Thus, it is important to understand their behavior, as they become integrated into western wildlife communities. However, little research has been conducted on the ecology of feral horses, and their relationships with native ungulates, carnivores, and local resources such as water.

To address these concerns, I outlined three objectives: (1) determine if horses changed their water visitation activity times in relation to temperature. As a primarily diurnal species, we were interested …

An Analysis Of Targeted Physical Therapy Against Medial Epicondylitis, Rylie Howe

Undergraduate Honors Capstone Projects

Medial epicondylitis, more commonly known as "golfer's elbow", is a musculoskeletal condition involving the common flexor tendon in the forearm at the attachment point of the medial epicondyle of the humerus bone. The repeated use of this tendon causes inflammation, swelling, as well as other types of straining that results in minute damage that accumulates to the overall strain and complications that come with medial epicondylitis. Targeted physical therapy is the overall recommended treatment for this condition. However, there is limited consensus to the most effective interventions. Mixed methods were used to investigate the possibility of the success of certain …

Testing Resilience Of Microalgae Biofilms When Exposed To Hydrothermal Liquefaction Aqueous Phase, Davis Riley Haag

Undergraduate Honors Capstone Projects

There are a multitude of experimental and processing techniques in the bioenergy field that produce sustainable and carbon negative to carbon neutral methods of converting renewable resources into biologically based hydrocarbons. One example of a process is hydrothermal liquefaction (HTL) which specializes in converting wet biomass into biocrude oil. One reason that these processes are not used outside experimental testing is due to the high cost of performing these processes. There is potential to move these processes to larger scale testing and eventually industrial adaptation if breakthroughs are made to offset the process expenses. There is a waste product produced …

Variable Drought Impacts On Stream Habitat Across The Northwestern United States, Sophia Withers

Undergraduate Honors Capstone Projects

The Western United States has been in an extended drought since 2002 which can drive negative impacts on stream habitats supporting native fish and aquatic species. Stream quality, specifically in the context of fish habitat, depends on many variables, including vegetation quality. Drought conditions affect vegetation conditions; however it is unclear how drought conditions impact stream habitat quality for fish. This study examines how stream habitat quality relates to drought across the National Forests of the Northwestern US, as well as exploring whether drought increases have had a significant impact on riparian vegetation over the past 20 years. Using a …

Chemical-Mediated Mate Attraction In Clover Root Curculio (Sitona Hispidulus F.), A Belowground Pest Of Forage Crops, Kristin Leutgeb

Undergraduate Honors Capstone Projects

Curculionid beetles are well known for their production of semiochemicals, including contact (e.g. cuticular hydrocarbons) and volatile pheromones that mediate conspecific attraction and mating. Clover root curculio (CRC; Sitona hispidulus) in particular is an emergent, soil-borne pest of significant concern to producers of alfalfa and other leguminous crops in the United States. Producers have few control options for CRC and a greater understanding of chemical-mediated attraction in this species can potentially improve the sustainability of its management. To examine which chemical cues are important for affecting the behavior of CRC and whether there are sex-based differences in response, I …

Post-Activation Potentiation Following Maximal Effort, Multi-Joint, Isokinetic Eccentric Contractions, Tom Dickey

All Graduate Reports and Creative Projects, Fall 2023 to Present

Post-activation potentiation (PAP) is a phenomenon that can enhance muscle performance following maximal or near-maximal muscle contractions. While the effects of concentric and isometric conditioning contractions on PAP have been studied, less is known about the influence of eccentric muscle actions. This study investigated the effects of a multi-joint eccentric overload (EOL) protocol on PAP expressed through countermovement jump (CMJ) height and isokinetic peak force (PF) outcome measures. Twenty-eight recreationally trained participants (18-30 years) completed three visits in a randomized, counterbalanced design. Following familiarization, participants performed either an EOL protocol involving 2 sets of 6 maximal isokinetic eccentric contractions or …

Asymmetries In Ncaa Division I Tennis Players Compared To An Athletic Control Group, Elizabeth Cafferty

All Graduate Reports and Creative Projects, Fall 2023 to Present

Limb asymmetries are an expected adaptation to years of training for athletes participating in dominant-sided sports. Previous research on this topic lacks an athletic control group. PURPOSE: To determine the magnitude of upper limb asymmetries in dominant-sided athletes (tennis players) compared to nondominant-sided athletes (cross-country runners). METHODS: Male and female NCAA Division I athletes (10 tennis, 11 cross-country) participated. Dual-energy x-ray absorptiometry (DXA) was used to measure bone mineral content (BMC), bone mineral density (BMD), and lean mass (LM) of the whole body, upper extremities, and forearms. Circumference measurements were taken at mid-biceps and the widest part of the forearms. …

Characterizing The Effect Of Field-Relevant Levels Of Imidacloprid On Respiration In The Common Flesh Fly, Sarcophaga Bullata Parker (Diptera: Sarcophagidae), During Low- And High-Energy Activities., Morgan Regnier Ms ' 24

All Student Scholarship

Neonicotinoid pesticides are used worldwide due to their effectiveness in protecting crops from pests like aphids, thrips, moths, flies, and beetles. These insecticides are applied on surfaces or absorbed systemically by plants, where insects encounter them through contact or ingestion. While beneficial for agriculture, they also negatively impact nontarget species. Numerous studies have reported the inhibitory effects of sublethal exposure to neonicotinoid-based insecticides on nontarget insects, especially pollinators such as bees, flies, and beetles. While research has demonstrated the toxicity of neonicotinoids, most studies have focused on low-energy activities such as walking or resting. In this research, I examined the …

Baseline Assessment And Early Effects Of A Network Of Marine Protected Areas, Victor Brun, Laurre Thierry De Ville D'Avray, Ma. May Saludsod, Roger D. Dolorosa, Niño Jess Mar F. Mecha, Kent Olson S. Sorgon, Joachim Claudet

Biology Faculty Publications

Marine protected areas (MPAs) can be a useful tool to manage coastal ecosystems, delivering both social and ecological outcomes. However, in many instances, relevant data is missing to conduct proper impact assessments, which is key to identifying ecological and social synergies and potential trade-offs, and to adapting management according to local objectives. The ecological effects of an MPA established in Palawan, Philippines, in 2016, were assessed. The most common species targeted by fisheries were identified by local fishers. Species size and number were collected through underwater visual census with n = 288 belt transects assigned in different sites and locations …

Influence Of Four Veterinary Antibiotics On Constructed Treatment Wetland Nitrogen Transformation, Matthew Russell, Tiffany L. Messer, Deborah A. Report, Richard L. Smith, Shannon L. Bartelt-Hunt, Daniel D. Snow, Ariel P. Reed

UK CARES Faculty Publications

The use of wetlands as a treatment approach for nitrogen in runoff is a common practice in agroecosystems. However, nitrate is not the sole constituent present in agricultural runoff and other biologically active contaminants have the potential to affect nitrate removal efficiency. In this study, the impacts of the combined effects of four common veterinary antibiotics (chlortetracycline, sulfamethazine, lincomycin, monensin) on nitrate-N treatment efficiency in saturated sediments and wetlands were evaluated in a coupled microcosm/mesocosm scale experiment. Veterinary antibiotics were hypothesized to significantly impact nitrogen speciation (e.g., nitrate and ammonium) and nitrogen uptake and transformation processes (e.g., plant uptake and …

Mathematical Evaluation Of Population Changes Of Lactobacillus Acidophilus And Bifidobacterium Animalis Ssp. Lactis As Free And Encapsulated Cells In Butter, Rakesh Kaushik, Kritika Gaba, Sanjeev Anand, Gemechis Djira

Dairy Science and Food Science Faculty Publications: 2022 to Present

Growing butter markets, domestically and globally, provide opportunities for value-added variants of butter. Adding probiotics to butter could boosts its bioactivity; however, maintaining probiotic viability during storage is a major challenge. Mathematical analysis of probiotic population changes could help improve our understanding of how probiotics interact with butter and storage conditions. Two strains of probiotics in a 1:1 ratio as free cells or Whey Protein Hydrolysate–Maltodextrin (WPH-MD)-encapsulated cells, Lactobacillus acidophilus ATCC 4356 (LA5) and Bifidobacterium animalis ssp. lactis ATCC 27536 (BB12), were separately mixed into butter at 1% levels. Using analysis of covariance, a mathematical evaluation for probiotic population changes …

Utilizing Biomimicry To Design Sustainable Architecture, Virginia Hammond

Architecture Undergraduate Honors Theses

Nature has an integral relationship with architecture and serves as a sustainable role model and inspiration for designers. The process of biomimicry in architecture has the potential to produce more sustainable design solutions and foster a connection between humans and nature. Existing biomimetic design projects have varying strengths and weaknesses as examples of the process. Utilizing guidelines and references from key leaders in biomimetic design consultancy (Biomimicry 3.8), selected case studies are assessed for their ability to demonstrate the benefits of this design strategy. Using these evaluations, the case studies are diagrammed and critiqued to determine how new projects could …

Transcriptomic Profiling Of Engineered Human Gene Integration In The Mouse Genome Following In Vitro Gene Editing, Ethan Potts, Made Harumi Padmaswari, Christopher Nelson

Biomedical Engineering Undergraduate Honors Theses

Gene replacement is a promising method of therapy for genetic diseases. However, safety and efficacy are areas that need more research. This experiment aims to use RNA sequencing and bioinformatic techniques to provide answers to these questions and provide direction to future studies to develop a gene replacement therapeutic. C2C12 mouse myoblast cells were transfected with a vector containing a CRISPR-Cas9 system and the Human Factor IX (hF9) gene in order to hijack target genes and integrate the hF9 gene. The two target genes, myoglobin (Mb) and creatine kinase (Ckm), were chosen for their high rate of expression and low …

Evaluating The Epidemiological, Hormonal, And Psychosocial Sex-Based Differences In Sports-Related Concussion Outcomes, Samantha Kanny

All Dissertations

Sports-related concussions (SRCs) affect approximately 3.8 million athletes in the United States per year. A multitude of risk factors including age, sex, concussion history and prior medical conditions have been observed to significantly increase sports-related concussion symptom burden. Researchers have posited many reasons to explain sex-based differences in SRC outcomes, often categorized by biological characteristics or sociocultural factors, however, females are vastly underrepresented in sports-related concussion research, making it difficult to understand the true mechanisms underlying SRC sex-based differences. The goal of this dissertation is to help fill the current gap in knowledge on sex-based differences in sports-related concussion outcomes. …

Demography With Drones: Detecting Growth And Survival Of Shrubs With Unoccupied Aerial Systems, Peter J. Olsoy, Andrii Zaiats, Donna M. Delparte, Matthew J. Germino, Bryce A. Richardson, Anna V. Roser, Jennifer S. Forbey, Megan E. Cattau, T. Trevor Caughlin

Biology Faculty Publications and Presentations

Large-scale disturbances, such as megafires, motivate restoration at equally large extents. Measuring the survival and growth of individual plants plays a key role in current efforts to monitor restoration success. However, the scale of modern restoration (e.g., >10,000 ha) challenges measurements of demographic rates with field data. In this study, we demonstrate how unoccupied aerial system (UAS) flights can provide an efficient solution to the tradeoff of precision and spatial extent in detecting demographic rates from the air. We flew two, sequential UAS flights at two sagebrush (Artemisia tridentata) common gardens to measure the survival and growth of …

Intrinsically Disordered Proteins And Their Role In Biomolecular Condensates, Danielle Latham

All Dissertations

Proteins are biomacromolecules responsible for the functions of life. While classically proteins are thought to be well structured in order to perform a specific function, 50% of proteins within Eukaryotic cells contain intrinsically disordered regions (IDRs), regions with no well-defined structure. IDRs are often used for cell signaling, responding to external factors such as temperature changes or the presence of small molecules. To understand how IDRs can function without structure, it is important to understand the dynamics of such systems. Understanding IDR intramolecular and intermolecular interactions will shed light on IDR dynamics. Intramolecular interactions are first explored using fluorescence spectroscopy …

Managing Barley Powdery Mildew In The Face Of Fungicide Resistance, Department Of Primary Industries And Regional Development, Western Australia

Grains and other field crops factsheets

Barley powdery mildew populations resistant to some fungicide active ingredients are present in the WA grainbelt. Integrated disease management strategies to manage the disease include growing varieties with improved resistance and strategic use of fungicides.

Live Cattle Assessment, Department Of Primary Industries And Regional Development, Western Australia

Animal production and livestock factsheets

Cattle producers regularly make visual assessments of their cattle. Frame, structure, muscularity, fatness and condition score can all be assessed by visual appraisal. Some traits cannot be assessed visually (for example fertility or marbling). To evaluate the genetic merit of an animal, estimated breeding values (EBVs) can be used.

Water Quality For Livestock, Department Of Primary Industries And Regional Development, Western Australia

Animal production and livestock factsheets

Measure water quality and quantity to effectively plan and monitor water supplies for livestock. If water quality is poor, livestock may drink less than they need, or rarely, may stop drinking altogether. When animals drink less, they will eat less and lose condition, and if they are lactating, their milk production will reduce or cease.

Water quality for livestock in Western Australia is most affected by water salinity, and the presence of water contaminants such as blue-green algae, organic material, heavy metals and chemicals.

Barley Production: Effects Of Weather Damage And Varietal Purity On Quality, Department Of Primary Industries And Regional Development, Western Australia

Grains and other field crops factsheets

Australian barley is regarded for its quality and suitability for both malting and animal feed, although varietal differences and environmental conditions during production can influence its quality.

Near-Surface Soil Property Change Over Time In Two Native Tallgrass Prairies In The Arkansas River Valley, Katie Jansson

Crop, Soil and Environmental Sciences Undergraduate Honors Theses

One climate-change mitigation pathway is soil carbon (C) sequestration, which removes carbon dioxide from the atmosphere and stores C in various forms in the soil. Native prairie soils have long been known for their soil C sequestration abilities, but the full extent of their sequestration capacity is not completely understood. This study evaluated the effect of soil depth and soil map unit/prairie combination on changes in various soil properties over time from 2018 to 2022 in the top 20 cm of two native tallgrass prairie soils in the Arkansas River Valley in west-central Arkansas. Soil samples were collected from the …

One Year Follow Up Of A Cross-Sectional Study Of Hookworm Infection And Response To Deworming In Beposo, Ghana, Taylor Clarkson

Capstone Experience: Master of Public Health

Hookworms are soil-transmitted helminths that infect over 500 million people worldwide, primarily those in low-income countries. Infection with hookworm can cause anemia, malnutrition, and stunted physical and cognitive development. Deworming campaigns have attempted to combat hookworm infections in endemic areas for decades. However, there is growing variability in the effectiveness of deworming drugs, and post-treatment reinfections are abundant. There are significant gaps in our understanding of critical host-parasite factors that mediate hookworm infection, reinfection, and response to deworming treatment in endemic communities. A five-year prospective study was undertaken to better understand drivers of hookworm infection in rural Ghana and thus …

Towards Understanding The Function Of An Ets-Like Gene In Nematostella Vectensis: Generation Of A Knockout Mutant Line And A Transgenic Reporter Line, Emily Bullock

Biological Sciences Undergraduate Honors Theses

Due to their unique phylogenic position as sister to Bilateria, Cnidaria are often credited with the utility of allowing for reconstruction of ancestral biology based on characteristics shared with bilaterians and other animals. This factor makes investigation into the nervous systems of cnidarians critical in understanding early neural evolution. Wamides, a class of neuropeptides, have been shown to play a regulatory role in life cycle transitions across many different species. The cnidarian specific Wamide neuropeptide, GLWamide, has previously been identified to play an accelerator role in the metamorphic timing of a specific species of sea anemone, Nematostella vectensis. However, …

Establishing An Objective, Quantifiable Method For Detecting Erosive Tooth Wear, Camille Kita

Biological Sciences Undergraduate Honors Theses

This study aims to monitor and evaluate erosive tooth wear (ETW) by measurement of enamel surface thickness and texture in a clinical setting. The objective approach is fundamentally different from the standard subjective visual assessment that professionals in the dental field using the Basic Erosive Wear Evaluation (BEWE) method.

Twenty-nine hyposalivation and three control subjects were used in this longitudinal study, for a total of 597 samples (only 577 teeth were eligible for surface texture analysis) evaluated in a longitudinal study including both baseline and 12 month follow up surfaces. Each participant’s buccal surface of the tooth was first analyzed …

Characterization Of Mitochondrial Morphology In Drosophila: Early Embryogenesis Description Via Confocal Microscopy, Kendall Miller

Biological Sciences Undergraduate Honors Theses

Mitochondria are not static organelles, but instead are dynamic networks that undergo rapid changes in subcellular distribution, organization, and activity in response to external stimuli. Notably, mitochondria can undergo fission and fusion, collectively known as mitochondrial dynamics. Mitochondrial fusion creates assembled networks of mitochondria that are maximally efficient at generating ATP through oxidative phosphorylation. Conversely, mitochondrial fission leads to network fragmentation and a shift towards glycolysis, which is the dominant mode of ATP generation in oxygen-limiting and stressed conditions. While mitochondrial dynamics have been intensively studied in isolated cells under stressful conditions, much less is known about how mitochondria behave …

Harnessing Oyster Shells For Sustainable Coastal Restoration, Resilience And Ecosystem Enhancement, Falyn Mcquarrie

Capstone Projects

Climate change and coastal erosion are two major threats facing coastal wetlands and marshes, especially in Connecticut and Rhode Island. Climate change has been causing sea level rise and increased storm surge during natural disasters like hurricanes and tropical storms, exacerbating erosion. Improper management techniques are also a challenge that shorelines are facing, hard structures like seawalls can be expensive to maintain and can lead to further erosion. Living shorelines is an adaptive management approach that can be beneficial to climate change and erosion mitigation and can foster nursery environments that invite new species and juvenile fish to the area …