Life Sciences Commons^™

Functional Genomics Approaches To Understanding The Impact Of Common And Rare Risk Variants On Microglia Function In Neurodegenerative Diseases, Shih-Feng You

Arts & Sciences Graduate Student Theses and Dissertations

Genome-wide association studies (GWAS) have identified more than 70 genetic loci that are linked to Alzheimer disease (AD) risk. Many of these risk genes are involved in microglial function, including MS4A4A, TREM2, and PLD3, suggesting a relationship between microglial phagocytosis, metabolism, inflammation, and AD pathogenesis. However, the mechanisms by which AD risk genes alter microglia function and contribute to disease remains inadequately explored. In this dissertation, I leveraged functional genomics methods to understand the impact of MS4A4A, TREM2, and PLD3 on microglia function in stem cell and mouse models as well as human brain tissue. MS4A4A is a microglial gene …

Mitochondrial Network Reorganization And Transient Expansion During Oligodendrocyte Generation, Xhoela Bame, Robert A. Hill

Dartmouth Scholarship

Oligodendrocyte precursor cells (OPCs) give rise to myelinating oligodendrocytes of the brain. This process persists throughout life and is essential for recovery from neurodegeneration. To better understand the cellular checkpoints that occur during oligodendrogenesis, we determined the mitochondrial distribution and morphometrics across the oligodendrocyte lineage in mouse and human cerebral cortex. During oligodendrocyte generation, mitochondrial content expands concurrently with a change in subcellular partitioning towards the distal processes. These changes are followed by an abrupt loss of mitochondria in the oligodendrocyte processes and myelin, coinciding with sheath compaction. This reorganization and extensive expansion and depletion take 3 days. Oligodendrocyte mitochondria …

Recurrent Inflammatory Myofibroblastic Tumor Of Larynx Harboring A Novel Thbs1::Alk Fusion, Namra Ajmal, Stacey Gargano, Ujwala Gosavi, Madalina Tuluc

Computational Medicine Center Faculty Papers

Inflammatory myofibroblastic tumor (IMT) is a rare soft tissue tumor primarily occurring in the abdominopelvic region of young patients, and it is characterized by spindle-shaped myofibroblasts, or fibroblasts surrounded by inflammatory infiltrate. Herein, we report a case of a 24-year-old male with a firm submucosal mass in the anterior right vocal fold diagnosed as an IMT that recurred 14 months later. The tumor demonstrated a novel THBS1::ALK fusion containing Exons 1-7 of the thrombospondin 1 (THBS1) gene fused to Exon 19 of the anaplastic lymphoma kinase (ALK) gene via next-generation sequencing with the NextSeq sequencer. The fusion of THBS1 to …

Role Of The Antiparallel Double-Stranded Filament Form Of Ftsa In Activating The Escherichia Coli Divisome, Abbigale Perkins, Mwidy Sava Mounange-Badimi, William Margolin

Faculty, Staff and Student Publications

The actin-like FtsA protein is essential for function of the cell division machinery, or divisome, in many bacteria including Escherichia coli. Previous in vitro studies demonstrated that purified wild-type FtsA assembles into closed mini-rings on lipid membranes, but oligomeric variants of FtsA such as FtsAR286W and FtsAG50E can bypass certain divisome defects and form arc and double-stranded (DS) oligomeric states, respectively, which may reflect conversion of an inactive to an active form of FtsA. However, it remains unproven which oligomeric forms of FtsA are responsible for assembling and activating the divisome. Here, we used an in vivo crosslinking assay …

Production, Purification, And Specificity Serologically Determination Of Immunoglobulin-Y (Igy) From Chicken Eggs Against Clostridium Tetani Toxoid, Kürşat Teti̇k, Bariş Sareyyüpoğlu

Turkish Journal of Veterinary & Animal Sciences

Tetanus is a life-threatening bacterial disease of humans and many animal species caused by the neurotoxin tetanospasmin (TeNT) produced by Clostridium tetani. Antitoxins obtained from horses and humans are primarily used to treat this disease. However, there are several clinical side effects and disadvantages associated with the use of these antitoxins. Current techniques for diagnosing tetanus use monoclonal antibodies produced in mice. These antibodies have several advantages, such as homogeneity and specificity. In contrast, a notable feature of polyclonal antibodies, especially egg yolk antibodies (IgYs) extracted from poultry, is that they can be generated in greater quantities than mammalian antibodies …

An Intronic Regulatory Element Limits Tcf7 Expression In Natural Killer Cells, Matthew Vincent Mccullen

Arts & Sciences Graduate Student Theses and Dissertations

To provide protection against a broad spectrum of threats, the immune system must give rise to a diverse range of cell types each with specialized roles in host defense. Transcriptional regulation is achieved by interactions between transcription factors and non-coding cis-regulatory DNA elements such as promoters and enhancers. One of the most important transcription factors in lymphocyte development and function is TCF1 (encoded by the Tcf7 gene). TCF1 is required for the efficient development of both innate lymphocytes, such as NK cells, as well as adaptive T cells. TCF1 also regulates the functional abilities of these cell types, such as …

Predicting Cell Fate Using Single-Cell State And Lineage Information, Emily Grace Butka

Arts & Sciences Graduate Student Theses and Dissertations

Humans begin as a single cell, with successive cell divisions ultimately giving rise to a complex organism comprised of innumerable highly specialized cells. In this process, cells begin as pluripotent stem cells and slowly shift towards these highly specialized identities. Several technological innovations have enabled scientists to measure and track many aspects of a single cell’s identity as these divisions occur, at increasingly large scale and rapidly declining cost. As a result, our fundamental understanding of cell identity has grown to encompass several layers of cellular dynamics, including aspects that are both intrinsic and extrinsic, transient and permanent, active and …

Insights From Open Conformations Of Ecmsck, Htrpv4, And Hhcn1 Into The Structural Basis Of Ligand-Regulated Ion Channel Gating, Jonathan Mount

Arts & Sciences Graduate Student Theses and Dissertations

Ion channels are found in all domains of life and play fundamental roles in cellular signaling and response to environmental cues. The ability to accommodate the selective and rapid passage of ions across a cell membrane is conferrable by a myriad of protein folds with no singular one-size-fits-all approach. With the advent of the cryo-EM resolution revolution, substantial interest has developed for determining the molecular structures of conformational states that correspond to conductive and nonconductive forms of ion channels. By gaining insight into the structural mechanisms for ion channel gating, we can potentially reveal binding pockets for targeted small molecule …

Hyperpolarized Magnetic Resonance Imaging, Nuclear Magnetic Resonance Metabolomics, And Artificial Intelligence To Interrogate The Metabolic Evolution Of Glioblastoma, Kang Lin Hsieh, Qing Chen, Travis C Salzillo, Jian Zhang, Xiaoqian Jiang, Pratip K Bhattacharya, Shyan Shams

Faculty, Staff and Student Publications

Glioblastoma (GBM) is a malignant Grade VI cancer type with a median survival duration of only 8-16 months. Earlier detection of GBM could enable more effective treatment. Hyperpolarized magnetic resonance spectroscopy (HPMRS) could detect GBM earlier than conventional anatomical MRI in glioblastoma murine models. We further investigated whether artificial intelligence (A.I.) could detect GBM earlier than HPMRS. We developed a deep learning model that combines multiple modalities of cancer data to predict tumor progression, assess treatment effects, and to reconstruct in vivo metabolomic information from ex vivo data. Our model can detect GBM progression two weeks earlier than conventional MRIs …

Persistence Of Daptomycin-Resistant And Vancomycin-Resistant Enterococci In Hospitalized Patients With Underlying Malignancies: A 7-Year Follow-Up Study, Lynn El Haddad, Georgios Angelidakis, Yuting Zhai, Layale Yaghi, Cesar A Arias, Samuel A Shelburne, Kwangcheol Casey Jeong, Roy F Chemaly

Faculty, Staff and Student Publications

Vancomycin-resistant enterococci (VRE) commonly colonize the gut of individuals with hematologic malignancies or undergoing hematopoietic cell transplant (HCT) and may cause bacteremia. In 2012, we identified VRE isolates from patients and patients' rooms and showed transmission networks of highly genetically related daptomycin-resistant (DR)-VRE strains. This is a follow-up study performing whole-genome sequencing (WGS) and phylogenetic analyses on 82 clinical VRE strains isolated from stools and blood cultures of patients with leukemia and HCT between 2015 and 2019. Here, we observed transmission of highly genetically related strains between rooms on the same or on different floors, including a DR-VRE strain identified …

Hybridizing Mechanistic Modeling And Deep Learning For Personalized Survival Prediction After Immune Checkpoint Inhibitor Immunotherapy, Joseph D Butner, Prashant Dogra, Caroline Chung, Eugene J Koay, James W Welsh, David S Hong, Vittorio Cristini, Zhihui Wang

Faculty, Staff and Student Publications

We present a study where predictive mechanistic modeling is combined with deep learning methods to predict individual patient survival probabilities under immune checkpoint inhibitor (ICI) immunotherapy. This hybrid approach enables prediction based on both measures that are calculable from mechanistic models of key mechanisms underlying ICI therapy that may not be directly measurable in the clinic and easily measurable quantities or patient characteristics that are not always readily incorporated into predictive mechanistic models. A deep learning time-to-event predictive model trained on a hybrid mechanistic + clinical data set from 93 patients achieved higher per-patient predictive accuracy based on event-time concordance, …

Alternate Conformational Trajectories In Ribosome Translocation, Jose L. Alejo, Dylan Girodat, Michael J. Hammerling, Jessica A. Willi, Michael C. Jewett, Aaron E. Engelhardt, Katarzyna P. Adamala

Chemistry & Biochemistry Faculty Publications and Presentations

Abstract

Translocation in protein synthesis entails the efficient and accurate movement of the mRNA-[tRNA]₂ substrate through the ribosome after peptide bond formation. An essential conformational change during this process is the swiveling of the small subunit head domain about two rRNA ‘hinge’ elements. Using iterative selection and molecular dynamics simulations, we derive alternate hinge elements capable of translocation in vitro and in vivo and describe their effects on the conformational trajectory of the EF-G-bound, translocating ribosome. In these alternate conformational pathways, we observe a diversity of swivel kinetics, hinge motions, three-dimensional head domain trajectories and tRNA dynamics. By finding …

Regulatory Complexity Of Cellular Differentiation In Candida Albicans Revealed Through Systematic Screening Of Protein Kinase Mutants, Michael C Lorenz

Faculty, Staff and Student Publications

A recent study in mBio reports the construction and preliminary screening of a library containing mutants of 99 of the 119 predicted protein kinases in Candida albicans (the majority of the remaining 20 are probably essential) (J. Kramara, M.-J. Kim, T. L. Ollinger, L. C. Ristow, et al., mBio e01249-24, 2024, https://doi.org/10.1128/mbio.01249-24). Using a quantitative competition assay in 10 conditions that represent nutritional, osmotic, cell wall, and pH stresses that are considered to model various aspects of the host environment allowed them to phenotypically cluster kinases, which highlight both the integration and specialization of signaling pathways, suggesting novel functions …

Assessing Patterns Of Metazoans In The Global Ocean Using Environmental Dna, Nathan R. Geraldi, Silvia G. Acinas, Intikhab Alam, Josep M. Gasol, María Luz Fernández-De-Puelles, Caterina R. Giner, Santiago Hernández León, Ramiro Logares, Ramon Massana, Pablo Sánchez, Vladimir Bajic, Takashi Gojobori, Carlos M. Duarte

Research outputs 2022 to 2026

Documenting large-scale patterns of animals in the ocean and determining the drivers of these patterns is needed for conservation efforts given the unprecedented rates of change occurring within marine ecosystems. We used existing datasets from two global expeditions, Tara Oceans and Malaspina, that circumnavigated the oceans and sampled down to 4000 m to assess metazoans from environmental DNA (eDNA) extracted from seawater. We describe patterns of taxonomic richness within metazoan phyla and orders based on metabarcoding and infer the relative abundance of phyla using metagenome datasets, and relate these data to environmental variables. Arthropods had the greatest taxonomic richness of …

Personalized Nutrition: Tailoring Dietary Recommendations Through Genetic Insights, Saiful Singar, Ravinder Nagpal, Bahram H. Arjmandi, Neda S. Akhavan

Kinesiology and Nutrition Sciences Faculty Research

Personalized nutrition (PN) represents a transformative approach in dietary science, where individual genetic profiles guide tailored dietary recommendations, thereby optimizing health outcomes and managing chronic diseases more effectively. This review synthesizes key aspects of PN, emphasizing the genetic basis of dietary responses, contemporary research, and practical applications. We explore how individual genetic differences influence dietary metabolisms, thus underscoring the importance of nutrigenomics in developing personalized dietary guidelines. Current research in PN highlights significant gene–diet interactions that affect various conditions, including obesity and diabetes, suggesting that dietary interventions could be more precise and beneficial if they are customized to genetic profiles. …

Simulating The Long-Term Response Of Forest Succession To Climate Change In The Boreal Forest Of Northern Ontario, Canada, Guy R. Larocque, F. Wayne Bell, Eric B. Searle, Stephen J. Mayor, Thomas Schiks, Parvin Kalantari

Aspen Bibliography

The effect of climate change on forest dynamics is likely to increase in importance in the forthcoming decades. For this reason, it is essential to predict the extent to which changes in temperature, precipitation, and atmospheric CO₂ might affect the development of forest ecosystems and successional pathways. The gap model ZELIG-CFS was used to simulate the potential long-term effects of climate change on species-specific annual change in mean basal area and stand density under two scenarios of representative concentration pathways (RCP), 4.5 and 8.5, for the boreal forest region of Ontario, Canada, where mean temperature, precipitation, and atmospheric CO …

Investigating The Cellular Mechanisms Of Intracellular Ph Regulation During Exposure To High Co2., Dillon Flowers

Biology Theses

There are at least two different strategies of pH regulation among fish. The first is coupled pH regulation (CPR), where regulation of blood pH facilitates tissue pH regulation; and the second is preferential intracellular pH (pHi) regulation (PPR), where tissue pH remains tightly regulated despite a sustained reduction in blood pH. The cellular and molecular mechanisms underlying the differences in pHi regulation are currently unknown. I investigated the mechanisms of pH regulation in fishes using Rainbow Trout (Oncorhynchus mykiss) and Channel Catfish (Ictalurus punctatus) which use CPR and PPR, respectively. Fish were exposed to elevated CO …

Insulin-Independent Regulation Of Type 1 Diabetes Via Large Brown Adipocyte-Secreted Proteins And The Novel Glucagon Regulator, Nidogen2, Jeongmin Lee

Arts & Sciences Graduate Student Theses and Dissertations

Current treatments for type 1 diabetes (T1D) focus on insulin replacement. We demonstrate the therapeutic potential of a secreted protein fraction from embryonic brown adipose tissue (BAT), independent of insulin. The large molecular weight secreted fraction mediates insulin receptor-dependent recovery of euglycemia in a T1D animal model, nonobese diabetic (NOD) mice, by suppressing glucagon secretion. This fraction also promotes white adipocyte differentiation and browning, maintains healthy BAT, and enhances glucose uptake in adipose tissue, skeletal muscle, and liver. From this fraction, we identify nidogen-2 as a critical BAT-secreted protein that reverses hyperglycemia in NOD mice, inhibits glucagon secretion from pancreatic …

Northern Fur Ssal Vibrissae Organochlorine Contaminant Concentrations, Dimitri Giarikos, Amy Hirons, Courtney White

SECLER Data

The eastern Pacific stock of northern fur seals (Callorhinus ursinus) on the Pribilof Islands has experienced population declines since 1980. The decline may be due to anthropogenic persistent organic pollutants (POPs) which bioaccumulate, are toxic, and persistent. This study conducted a comparison of 21 organochlorine (OC) and 39 polybrominated diphenyl ether (PBDE) analyte concentrations utilizing archived vibrissae (whiskers) from individual fur seals sampled in 1993 (n=30) and 2013 (n=41). The PBDEs had values five times greater than the legacy status OCs. There were no significant mean concentration differences between 1993 and 2013 for total organochlorines (ΣOCs), or total …

Northern Fur Seal Vibrissae Polybrominateddiphenylether Concentrations, Dimitri Giarikos, Amy Hirons, Courtney White

SECLER Data

The eastern Pacific stock of northern fur seals (Callorhinus ursinus) on the Pribilof Islands has experienced population declines since 1980. The decline may be due to anthropogenic persistent organic pollutants (POPs) which bioaccumulate, are toxic, and persistent. This study conducted a comparison of 21 organochlorine (OC) and 39 polybrominated diphenyl ether (PBDE) analyte concentrations utilizing archived vibrissae (whiskers) from individual fur seals sampled in 1993 (n=30) and 2013 (n=41). The PBDEs had values five times greater than the legacy status OCs. There were no significant mean concentration differences between 1993 and 2013 for total organochlorines (ΣOCs), or total …

Association Of Genetic Ancestry With Molecular Tumor Profiles In Colorectal Cancer, Brooke Rhead, David M Hein, Yannick Pouliot, Justin Guinney, Francisco M De La Vega, Nina N Sanford

Faculty, Staff and Student Publications

Background: There are known disparities in incidence and outcomes of colorectal cancer (CRC) by race and ethnicity. Some of these disparities may be mediated by molecular changes in tumors that occur at different rates across populations. Genetic ancestry is a measure complementary to race and ethnicity that can overcome missing data issues and better capture genetic similarity in admixed populations. We aimed to identify somatic mutations and tumor gene expression differences associated with both genetic ancestry and imputed race and ethnicity.

Methods: Sequencing was performed with the Tempus xT NGS 648-gene panel and whole exome capture RNA-Seq for 8454 primarily …

Gnotobiotic Mouse Models For Characterizing The Role Of The Microbiota On Undernutrition And Early Postnatal Brain Development, Alexandra Elizabeth Byrne

Arts & Sciences Graduate Student Theses and Dissertations

Undernutrition is a serious global health challenge; it accounts for nearly half of all mortality for children under the age of 5. Worse, undernutrition is compounded by intergenerational inheritance and can be transmitted from mother to offspring. The burden of undernutrition is unequally distributed around the world, with South Asia and Sub-Saharan Africa accounting for almost 60% of the global childhood stunting. Undernutrition is detrimental to many aspects of development, including long-term deficits in neurodevelopment, and current interventions rarely ameliorate long-term cognitive and motor deficits. Therefore, other causes of undernutrition need to be elucidated in order to treat this epidemic …

Investigation Of Photoperiodic Control Of Hypocotyl 1’S Role In Promoting Phytochrome B-Photobody Phase Separation, Sarah Anne Pardi

Arts & Sciences Graduate Student Theses and Dissertations

Light is arguably the most important environmental stimuli for all of plant growth and survival. Not only does light serve as an energy source via photosynthesis, it also serves as a signal for developmental programming. Plants perceive light through light-responsive proteins termed photoreceptors. Phytochromes are conserved red/far-red light photoreceptors. In the model plant Arabidopsis thaliana (thale cress), phytochrome B (phyB) is the primary red light photoreceptor. PhyB is involved in regulating almost all developmental processes throughout the plant’s life cycle, from seedling germination to flowering. In response to red light, phytochromes photoconvert to a biologically active form, transport from the …

Metal(Loid) Uptake And Physiological Response Of Coix Lacryma-Jobi L To Soil Potentially Toxic Elements In A Polluted Metal-Mining Area, Qun Zhao, Hongyan Liu, Longhua Wu, Peter Christie, Xuewen Wang, Ghulam Rasool, Guilan Peng

Faculty, Staff and Student Publications

Coix lacryma-jobi L. is a traditional medicinal plant in east Asia and is an important crop in Guizhou province, southwest China, where there are elevated levels of soil mercury and arsenic (As). Exposure to multiple potentially toxic elements (PTEs) may affect plant accumulation of metal(loid)s and food safety in regions with high geological metal concentrations. Field experiments were conducted to study the effects of PTEs on metal(loid) accumulation and physiological response of C. lacryma in different plant parts at three pollution levels. Total root length, number of root tips, number of branches, and number of root crosses increased with increasing …

The Biology And Function Of Extracellular Vesicles In Immune Response And Immunity, Raghu Kalluri

Faculty, Staff and Student Publications

Extracellular vesicles (EVs), such as ectosomes and exosomes, contain DNA, RNA, proteins and are encased in a phospholipid bilayer. EVs provide intralumenal cargo for delivery into the cytoplasm of recipient cells with an impact on the function of immune cells, in part because their biogenesis can also intersect with antigen processing and presentation. Motile EVs from activated immune cells may increase the frequency of immune synapses on recipient cells in a proximity-independent manner for local and long-distance modulation of systemic immunity in inflammation, autoimmunity, organ fibrosis, cancer, and infections. Natural and engineered EVs exhibit the ability to impact innate and …

Final Phase 1 Substudy Results Of Ivosidenib For Patients With Mutant Idh1 Relapsed/Refractory Myelodysplastic Syndrome, Courtney D Dinardo, Gail J Roboz, Justin M Watts, Yazan F Madanat, Gabrielle T Prince, Praneeth Baratam, Stéphane De Botton, Anthony Stein, James M Foran, Martha L Arellano, David A Sallman, Mohammad Hossain, Dylan M Marchione, Xiaofei Bai, Prapti A Patel, Stephanie M Kapsalis, Guillermo Garcia-Manero, Amir T Fathi

Faculty, Staff and Student Publications

Ivosidenib is a first-in-class mutant isocitrate dehydrogenase 1 (mIDH1) inhibitor with efficacy and tolerability in patients with advanced mIDH1 hematologic malignancies, leading to approval in frontline and relapsed/refractory (R/R) mIDH1 acute myeloid leukemia. We report final data from a phase 1 single-arm substudy of once-daily ivosidenib in patients with R/R mIDH1 myelodysplastic syndrome (MDS) after failure of standard-of-care therapies. Primary objectives were to determine safety, tolerability, and clinical activity. The primary efficacy end point was the complete remission (CR) + partial remission (PR) rate. Nineteen patients were enrolled; 18 were included in the efficacy analysis. Treatment-related adverse events occurred in …

A Comparative Analysis Of The Immunoglobulin Repertoire In Leukemia Cells And B Cells In Chinese Acute Myeloid Leukemia By High-Throughput Sequencing, Huige Yan, Lina Wu, Pingzhang Wang, Miaoran Xia, Zhan Shi, Xinmei Huang, Sha Yin, Qian Jiang, C Cameron Yin, Xiangyu Zhao, Xiaoyan Qiu

Faculty, Staff and Student Publications

It is common knowledge that immunoglobulin (Ig) is produced by B lymphocytes and mainly functions as an antibody. However, it has been shown recently that myeloblasts from acute myeloid leukemia (AML) could also express Ig and that AML-Ig played a role in leukemogenesis and AML progression. The difference between Ig from myeloblasts and B cells has not been explored. Studying the characteristics of the Ig repertoire in myeloblasts and B cells will be helpful to understand the function and significance of AML-Ig. We performed 5' RACE-related PCR coupled with PacBio sequencing to analyze the Ig repertoire in myeloblasts and B …

Prostate Cancer-Induced Endothelial-Cell-To-Osteoblast Transition Drives Immunosuppression In The Bone-Tumor Microenvironment Through Wnt Pathway-Induced M2 Macrophage Polarization, Guoyu Yu, Paul G Corn, Celia Sze Ling Mak, Xin Liang, Miao Zhang, Patricia Troncoso, Jian H Song, Song-Chang Lin, Xingzhi Song, Jingjing Liu, Jianhua Zhang, Christopher J Logothetis, Marites P Melancon, Theocharis Panaretakis, Guocan Wang, Sue-Hwa Lin

Faculty, Staff and Student Publications

Immune checkpoint therapy has limited efficacy for patients with bone-metastatic castration-resistant prostate cancer (bmCRPC). To improve immunotherapy for bmCRPC, we aimed to identify the mechanism of bmCRPC-induced changes in the immune microenvironment. Among bmCRPC patients, higher levels of a 32-gene M2-like macrophage signature in bone metastasis samples correlated with shorter overall survival. Immunohistochemistry showed that CD206-positive (CD206+) macrophages were enriched in bmCRPC bone biopsy specimens compared with primary tumors or lymph node metastases. In preclinical osteogenic prostate cancer (Pca) xenograft models, CD206+ macrophages were recruited to areas with tumor-induced bone. RNA sequencing (RNAseq) analysis showed higher expression of an M2-like …

Efficacy Of Probiotics In Decreasing Rates Of Necrotizing Enterocolitis And Sepsis In Preterm Neonates, Valisa K. Teekadharry

Theses and Graduate Projects

Background: The incidence of preterm infants developing serious infections is a common finding in many neonatal intensive care units. Probiotics have been a supplement that has been used by numerous people to improve the microbiota of the intestine for prevention of infection.

Purpose: The purpose of this literature review is to explore the efficacy of using probiotics in preterm infants for prevention of serious infections such as necrotizing enterocolitis (NEC) and sepsis.

Methods: A comprehensive literature review was conducted using Lindell Library, Pub Med, Science Direct and Google Scholar. Using the search terms probiotics, probiotics in premature infants, probiotics and …

Impact Of Partial Oil Removal On Energy Content Of Distillers Grains Plus Solubles For Finishing Cattle, Jordan E. Burhoop, J. L. Sperber, Curt J. Bittner, F. Henry Hilscher, J. C. Macdonald, Galen E. Erickson

Department of Animal Science: Faculty Publications

Two experiments evaluated the impact of the reduction in the percentage of corn oil remaining in distillers grains plus solubles (DGS) after the ethanol plant de-oiling process or by adding corn oil back to DGS following de-oiling on finishing cattle performance and nutrient digestion. Experiment 1 utilized 320 yearling steers (initial BW = 413 kg; SD = 25 kg) fed in 32 pens (10 steers/pen) and assigned to one of four treatments (n = 8 pens/treatment). The four treatments consisted of a blended DRC:HMC corn control diet (CON), de-oiled modified distillers grains plus solubles included at 40% of diet DM …