Life Sciences Commons^™

Investigation Of P27 Tumor Suppressor Gene (Cdkn1b) Polymorphisms In Dogs With Malignant Mammary Tumors, Gizem Kırmızıoğlu, Iraz Akış

The Thai Journal of Veterinary Medicine

Mammary tumors are the most common neoplasm in dogs, with a high mortality rate. The development of canine mammary tumors (CMT) is multifactorial, but different incidence rates between breeds suggest the effects of genetic risk factors. Many CMT-associated candidate genes were reported, mainly involved in cell cycle control. This study aims to investigate p27 tumor suppressor gene polymorphisms in dogs with mammary tumors and analyze the association between variations and CMTs. For this purpose, case and control groups were formed from 22 dogs diagnosed with malignant mammary tumors and 10 dogs with healthy mammary glands. The whole canine p27 gene …

Preventive Effects Of Quercetin On The Leaky Gut By Suppression Of Il-1beta-Induced Transcription Factors Involved In An Inflammatory Pathway In Colonic Epithelial Cells, Wannaporn Chayalak, Sumpun Thammacharoen, Chatsri Deachapunya, Sutthasinee Poonyachoti

The Thai Journal of Veterinary Medicine

Flavonol quercetin (Q), found in vegetables and fruits, demonstrates the most potent anti-inflammatory effect. The preventive effect and cellular mechanisms of Q (Q1, Q10, and Q100 µM) on IL-1β induced leaky gut via the signaling molecules of inflammatory pathway, NF-κB p65-iκB/or p38/ERK1/2/MLCK/phospho(p)-MLC were investigated in Caco-2 cells. The cytotoxic effect of Q was initially determined by MTT assay. Transepithelial electrical resistance (TER) and apical-to-basolateral fluorescein isothiocyanate-labeled dextran (FD-4) flux were used to determine paracellular permeability. The protein expression of signaling molecules was examined by Western blot analysis, and their target MLCK mRNA expression was determined using real-time PCR. All Q …

Declined Lawsonia Intracellularis In Feces By Phytogenic Feed Additive Supplementation In Fattening Pigs In Different Herds System, Morakot Nuntapaitoon

The Thai Journal of Veterinary Medicine

The objective of the present study was to determine the efficacy of phytogenic feed additives on the number of L. intracellularis in feces and incidence of diarrhea in fattening pigs in different herds system. The present study was carried out in two commercial swine farms located in the northern (Herd A) and western (Herd B) parts of Thailand. In total, 80 fattening pigs (12-week-olds) were randomly allocated into two groups, including the Control group (n=40) and the Treatment group (n=40). The pigs in each group were fed with a conventional diet (Control) and the same diet supplemented with 2 kg/ton …

The Molecular Detection Of Hemotropic Mycoplasma Species In Dogs, Hafize Tuğba Yüksel Dolgun, Şükrü Kirkan

The Thai Journal of Veterinary Medicine

Hemotropic mycoplasmas are uncultured bacteria that cause different degrees of hemolytic anemia in infected hosts. Molecular approaches are used to diagnose hemotropic mycoplasmas since they are difficult to identify using conventional methods. The aim of this study was to use molecular approaches to determine hemotropic mycoplasmas in dogs. Blood samples were collected in tubes (with EDTA) from 100 dogs. After DNA extraction, 100 DNA samples were investigated by PCR. Hemotropic mycoplasma was detected in 12 (12%) samples and positive samples were subjected to Sanger sequencing. Mycoplasma haemocanis was detected in 6 (50.0%) of the samples, Candidatus Mycoplasma hematoparvum in 5 …

Efficacy Of In-House Nanosilver Polyurethane Hydrogel Dressing On Second-Degree Burn Wounds In A Porcine Model, Pasakorn Brikshavana, Tapanat Songkasupa, Pleansaung Vichit, Onrampa Supsombon, Arunrode Nilrattanaprasert, Theerayuth Kaewamatawong

The Thai Journal of Veterinary Medicine

The aim of this study was to investigate the effects of hydrogel containing nanosilver particles on a second-degree burn wound in a porcine model. The second degree burn wound was performed using 1 cm in diameter, on 100 °C heated cylindrical brass bar applied to the dorsum of pig skin. Burn wounds were randomly divided into three groups of wound dressings (WDs) including commercial polyurethane hydrogel (Cutinova Hydro®, Gr. A), in-house hydrogel (Gr. B) and in-house nanosilver hydrogel (Gr. C). The wound contraction rate and hyperemic zone were measured and investigated at 3, 7, 14 and 21 days after dressing. …

In-House Polydimethylsiloxane Microfluidic Device On Single Cell Trapping And Culturing Of Leukemia Cell Line: Comparison Between Conventional And Conventional With Centrifugal Methods, Alongkorn Pimpin, Sudchaya Bhanpattanakul, Tepparit Wongpakham, Chomkwan Tawatcharaporn, Thanawit Siha-Umphai, Mookdapa Sinsiriphan, Atikan Chalermchuang, Tewan Tongmanee, Attawut Thanomsridetchai, Thammawit Suwannaphan, Witsaroot Sripumkhaie, Pattaraluck Pattamang, Mayuree Chanasakulniyom, Praprudee Piyaviriyakul, Wutthinun Jeamsaksiri, Achariya Sailasuta, Theerawat Tharasanit, Theerayuth Kaewamatawong

The Thai Journal of Veterinary Medicine

To develop a suitable model to study biological and characterization of Cancer stem cells (CSCs), single cell trapping and culturing using microfluidic techniques have become a high-through put system that can manage and control microenvironment and practical intracellular analysis. The aim of this study was to develop and validate a microfluidic device for single cell trapping and culturing using the leukemia cell line as a cell model. Comparison between conventional and conventional with centrifugal methods was also analyzed. To compare trapping efficacy between these 2 methods, leukemia cell concentration of 1.5×106 cells/ml was loaded with a flow rate of 0.25 …

Molecular Characterization And Antibiogram Of Riemerella Anatipestifer In Ducklings In Upper Egypt, Mohamed Sabry Ahmed, Amal Abd El-Ghani Batahi, Ahmed Ibrahim Ahmed, Nabila Osman

The Thai Journal of Veterinary Medicine

Riemerella anatipestifer infection represents a great threat to the duck industry worldwide and it is considered the major cause of death in the backyard rearing system of ducks in Egypt. The disease is characterized by diarrhea, respiratory signs and loss of weight. The present study was conducted during the period between July 2019 and January 2021 to examine the prevalence rate, the antibiogram patterns of the different antibiotics and the pathogenicity of R. anatipestifer in Qena province. One-hundred samples were collected from diseased and freshly dead backyard ducklings. A tentative characterization of R. anatipestifer was done depending on the phenotypic …

The Benefits Of Dexmedetomidine For Pain Mitigation After Cautery Disbudding In Calves, Tsung-Ching Liu, Chen-Fei Chiang, Thomas Chia-Tang Ho, Jacky Peng-Wen Chan

The Thai Journal of Veterinary Medicine

The purpose of this study was to determine the effect of dexmedetomidine (DEX) alone on pain mitigation following cautery disbudding in calves. A total of 21 calves were randomly divided into three groups: sham-disbudded control with placebo (S, n = 8); hot-iron disbudded after placebo (D, n = 6); hot-iron disbudded after 0.01 mg/kg of DEX (DeD, n = 7). The pain-related responses measured included serum cortisol levels, struggling scores, pain-related behaviors and other physiological values. The results indicated that the struggling scores, the cortisol concentrations from +10 to +60 min after disbudding and the area under curve of cortisol …

Productivity Of Young Broilers Fed Phyllanthus Niruri Linn And Melaleuca Cajuput Leaf Meal As A Phytogenic Feed Additive, Listya Purnamasari

The Thai Journal of Veterinary Medicine

Feed additives from medicinal plants can be used as alternative antibiotics to optimize productivity, growth, and health of livestock. Medicinal plants Phyllanthus niruri Linn (Meniran) and Melaleuca cajuput (Kajuput) as alternative growth promoters contain antioxidants and antimicrobials to improve the quality of animal products. The aim of the study was to evaluate the effect of medicinal plant leaf meals as phytogenic feed additives on broilers’ productivity and internal organ parameters. For 21 days fed, 80 1-day-old Loughmann broilers (MB202) unsex were distributed in 4 treatments with 4 pens per treatment and 5 birds per pen. The dietary treatments were: T1 …

Image Processing And Machine Learning Approach For Yolk Color Evaluation, Supreeya Thepparak, Chusak Pulmar, Chanwit Kaewtapee

The Thai Journal of Veterinary Medicine

The objective of this study was to develop a predictive model for yolk color measurement using machine learning. A total of eight hundred eggs were randomly collected from thirty laying hens (Lohmann brown, 28–35 weeks). The experimental diets were formulated based on broken rice or corn, and further supplemented with canthaxanthin from 10 to 150 mg/kg. The Digital YolkFan™ was used to classify yolk color scales from 0 to 15, whereas the Hunter Lab was used to measure color values. Furthermore, yolk images were obtained from a digital camera and then extracted into red color, green color and blue (RGB) …

Single-Tank Sequencing Batch Bioreactor With A Cellulose Substrate For Simultaneous Nitrification And Denitrification Of An Aquarium, Aranya Ponpornpisit, Malinee Jongjaroenjai, Naowarat Suthamnatpong, Sanit Piyapattanakorn, Surachet Burut-Archanai

The Thai Journal of Veterinary Medicine

Processing and detoxifying nitrogenous waste in an aquarium is difficult with one tank because nitrifying and denitrifying bacteria need contradictory conditions to grow and survive. Herein, a single-tank sequencing batch bioreactor was used to simultaneously perform aerobic nitrification and denitrification for aquarium filtration. To evaluate its performance at the laboratory-scale, the 8 L bioreactor was connected to a 20 L aquarium with a carbon water filter and containing Oranda goldfish. The bioreactor comprised a bottom layer filled with 2.5 L of polyester sponge, covered with 1 L of carbon pellets and then 2.5 L of cellulose beads or polyester sponge. …

Alternative Approaches To Live Attenuated Oral Rotavirus Vaccines, Makoye Mhozya Kanyema, Junhong Wang, Gui-Lian Yang, Xin Cao, Chunfeng Wang

The Thai Journal of Veterinary Medicine

Oral attenuated rotavirus vaccine is safe and effective in developed countries but, however, it is less effective in low income countries such as African and Asian countries. Low vaccine effectiveness in low-income countries is arising from a variety of sources such as maternal antibodies, chronic enteropathy and other enteric diseases such as cholera, typhoid, and parasitic diseases, as well as the microbiota. There is increasingly evidence related to the risk of intussusception for the oral vaccine. The development of effective and safer rotavirus vaccines, particularly, non-living candidates, is critical. The aim of this review therefore is to explore some of …

Phylotranscriptomic Relationships Of The Oophila Clade Of Green Algae Associated To Amphibian Egg Masses, Ryan Kerney, Miguel Vences, Maria Sachs, Iker Irisarri, Fabian Bartels, Pontus Eriksson, Sven Künzel, Atsushi Kurabayashi, Ane Laugen, Zachary Vegso, Cory Bishop, Hartmut Arndt

Biology Faculty Publications

Green algae usually assigned to the genus Oophila are known to colonize egg capsules of amphibian egg masses across the Nearctic and Palearctic regions. We study the phylogenetic relationships of these algae using a phylotranscriptomic data set of 76 protein-coding single-copy nuclear genes. Our data set includes novel RNAseq data for six amphibian-associated and five free-living green algae, and draft genomes of two of the latter. Within the Oophila clade (nested within Moewusinia), we find samples from two European frogs (Rana dalmatina and R. temporaria) closely related to those of the North American frog R. aurora (Oophila …

Optimal Limb Regeneration Strategies In Hemigrapsus Sanguineus, Laura S. Fletcher

Theses and Dissertations

Diet quality and energy allocation influence an individual’s life-history traits by determining how much energy is available for processes such as growth and reproduction. One common method of assessing diet quality involves measuring gut length or size, which is typically inversely related to diet quality. However, this often requires animal sacrifice and time- consuming dissections. Similarly, many traditional bioenergetics models fail to capture the context-dependence of energy allocation, as these models rely on fixed energy allocation rules. One such rule is the κ-rule of dynamic energy budget theory, which assumes a fixed proportion (κ) of assimilated energy is always allocated …

Software Solutions In Agri-Food Supply Chains: A Current View For Sustainability Reporting, Ryan Loy, Logan L. Britton, Trey Malone

Department of Agricultural Economics Faculty Publications

Environmental impact reporting has become a lynchpin for global agri-food supply chain decision-making. This importance has become heightened as governing bodies such as the European Union pass traceability legislation in an effort to reduce Scope 3 emissions. Despite this heightened importance, few studies have explored the role of supply chain management software in achieving sustainability goals within the agribusiness sector. This article provides a current perspective on ongoing supply chain and logistics software issues that might restrict the effectiveness of sustainability reporting. We argue that agribusinesses may struggle to achieve sustainability targets effectively without proficiency in supply chain software. This …

A Comparison Of Diploid And Triploid Eastern Oysters For Aquaculture Production Under Extreme Temperatures And Salinities, Joshua H. Kim

LSU Master's Theses

Off-bottom aquaculture of Eastern oysters (Crassostrea virginica) is a nascent industry that is increasingly supported by the use of triploid oysters, which grow faster than diploids. Despite their growth advantage, elevated triploid mortality compared to diploids under high temperature, low salinity, or a combination of these conditions challenge consistent triploid production. Identifying the environmental thresholds at which differential triploid mortality occurs and predicting its economic impact are important to informing decision-making in oyster aquaculture. The goal of this thesis was to compare the biological and economic performance of diploid and triploid oysters under high temperature and low salinity conditions. …

Improving Winter Survival Of Interspecific Hybrid Bermudagrass In The Mid-Atlantic Region Through Cultural Practices, Joseph R. Doherty, W. J. Hutchens, Jordan C. Booth, David S. Mccall, Michael V. Battaglia, Eric J. Deboer, Joseph A. Roberts

Horticulture Faculty Publications and Presentations

Winter injury can cause significant loss of hybrid bermudagrasses [Cynodon dactylon (L.) Pers. × C. transvaalensis Burtt-Davy] in the transition zone. Current research has focused on high-value, low-acreage areas like putting greens, but those practices are impractical to implement on golf course fairways. To that end, multi-year research projects were conducted at three sites across Maryland and Virginia to investigate the influence of annual N fertility [2.0 lb N 1000 ft⁻² (early summer application) or 4.0 lb N 1000 ft⁻² (split applied early and late summer)] across multiple fall mowing heights (0.5 inches, 0.8 inches, or 1 …

Microbial Hydrolysis Process To Improve Anaerobic Digestion For Biofuel Generation, Caleb Michael Jensen

Theses and Dissertations

Human development results in billions of tons of waste per year, which contributes to major issues such as pollution, climate change, and habitat destruction. In this study, we explored the impact of bacterial inoculation on volatile fatty acid (VFA) production, volatile solids (VS), and gas output in anaerobic digesters under different substrate treatments. Our results reveal that bacterial inoculation significantly boosts VFA levels, especially acetic acid, with both Caldicellulosiruptor bescii and mixed Caldicellulosiruptor, cultures showing notable differences compared to controls, especially in untreated manure and WAS treatments. Acetic acid and VS/TS increased in untreated WAS when inoculated with C. bescii …

Sounding The Alarm: Local Cues That Drive Functional Specialization Of Brain Resident Memory T Cells, Shawn Christopher Musial Jr.

Dartmouth College Ph.D Dissertations

Resident memory T cells are a unique subset of memory T cells that persist within tissues throughout the body, including the brain, optimally positioned to exert local immune activation. Broadly, T cells in the CNS are implicated in a range of neurologic disorders, can protect against reinfection, and even participate in normal CNS homeostasis. Despite this, there remain significant gaps in knowledge about how T cells, specifically resident memory T cells (T_RM), are regulated and function in the unique CNS environment. Here, we leverage a mouse model of acute viral infection to dissect the diversity of brain T …

Targeting Aspergillus Fumigatus Hypoxia Response Pathways For Novel Antifungal Drug Development., Cecilia Gutierrez Perez

Dartmouth College Ph.D Dissertations

Invasive fungal disease present are difficult to diagnose and treat and present a high mortality rate across the world with approximately 1.5 million people a year succumbing to fungal infections worldwide. Currently, there are limited antifungal classes used therapeutically, and antifungal drug resistance on the rise further exacerbates the dire need for novel therapeutics with innovative mode of actions. The filamentous fungi, Aspergillus fumigatus is the causative agent of invasive pulmonary aspergillosis (IPA). It is estimated that contemporary antifungals fail in about 50% of patients with invasive filamentous fungal infections, likely due to the biofilm mode of growth exhibited in …

Wild Parsnip (Pastinaca Sativa) Identification And Control In Residential Landscapes, Michael Caron, Taun Beddes, Corey Ransom

All Current Publications

Wild parsnip is a weed that can potentially cause serious injury to gardeners. This fact sheet describes this weed, its lifecycle, and suppression and control options.

Affinity Purification Mass Spectrometry Characterisation Of The Interactome Of Receptor Tyrosine Kinase Proline-Rich Motifs In Cancer, Christopher M Jones, Arndt Rohwedder, Kin Man Suen, Safoura Zahed Mohajerani, Antonio N Calabrese, Sabine Knipp, Mark T Bedford, John E Ladbury

Faculty, Staff and Student Publications

Receptor tyrosine kinase (RTK) overexpression is linked to the development and progression of multiple cancers. RTKs are classically considered to initiate cytoplasmic signalling pathways via ligand-induced tyrosine phosphorylation, however recent evidence points to a second tier of signalling contingent on interactions mediated by the proline-rich motif (PRM) regions of non-activated RTKs. The presence of PRMs on the C-termini of >40 % of all RTKs and the abundance of PRM-binding proteins encoded by the human genome suggests that there is likely to be a large number of previously unexplored interactions which add to the RTK intracellular interactome. Here, we explore the …

Hematopoietic Growth Factors Regulate The Entry Of Monocytes Into The Adult Brain Via Chemokine Receptor Ccr5, Xuefang Sophie Ren, Junchi He, Songruo Li, Heng Hu, Michele Kyle, Shinichi Kohsaka, Li-Ru Zhao

Faculty, Staff and Student Publications

Monocytes are circulating macrophage precursors generated from bone marrow hematopoietic stem cells. In adults, monocytes continuously replenish cerebral border-associated macrophages under physiological conditions. Monocytes also rapidly infiltrate the brain in pathological settings. The mechanisms of recruiting monocyte-derived macrophages into the brain under pathological conditions have been extensively studied. However, it remains unclear how monocytes enter the brain to renew border-associated macrophages under physiological conditions. Using both in vitro and in vivo approaches, this study reveals that a combination of two hematopoietic growth factors, stem cell factor (SCF) and granulocyte colony-stimulating factor (G-CSF), complementarily and synergistically enhances the adhesion of monocytes …

In Vitro Antibacterial, Antibiofilm Activities, And Phytochemical Properties Of Posidonia Oceanica (L) Delile: An Endemic Mediterranean Seagrass, Ertugrul Ozbil, Mehmet Ilktac, Sultan Ogmen, Ovgu Isbilen, Jesus M Duran Ramirez, Jana Gomez, Jennifer N Walker, Ender Volkan

Faculty, Staff and Student Publications

In the antibiotic resistance era, utilizing understudied sources for novel antimicrobials or antivirulence agents can provide new advances against antimicrobial resistant pathogens. In this study, we aimed to investigate antibacterial and antibiofilm activities of Posidonia oceanica (L.) Delile against Enterococcus faecalis ATCC 29212, Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922 and Klebsiella pneumoniae ATCC 700603 and several S. aureus clinical isolates obtained from medical devices, including patient urinary catheters and breast implant infections, with varying antibiotic recalcitrance profiles. The ethanolic and methanolic extracts from P. oceanica rhizome exhibited significant antibacterial activity against E. faecalis and S. aureus, as well …

An Indirect Elisa Using A Recombinant Envelope Protein For Detecting Antibody Against Duck Tembusu Virus Infection, Thaweesak Songserm

The Thai Journal of Veterinary Medicine

Duck Tembusu virus (DTMUV) belonging to Flavivirus group, causes severe central nervous system disorders and decreased egg production as found in Thailand. To detect antibody against the DTMUV infection, we developed an indirect enzyme-linked immunosorbent assay (ELISA) based on the recombinant envelop (E) protein for coating antigen. The E protein coding gene of KPS54A61DTMUV was cloned into pPICzαA vector and expressed by using 2% (v/v) of absolute methanol as inducer in Pichia pastoris expression. The recombinant E protein was detected in the culture medium at a concentration of 4.55 mg/ml and analyzed a protein expected size by SDS-PAGE at 65 …

Comparison Of Olympic And Safety Squat Bar Barbells On Force, Velocity, And Rating Of Perceived Exertion During Acute High-Intensity Back Squats In Recreationally Trained Men, Noah Staheli, Jeffrey C. Cowley, Marcus M. Lawrence

International Journal of Exercise Science

International Journal of Exercise Science 17(7): 1120-1133, 2024. This study examined using a traditional Olympic (OL) or safety squat bar (SSB) barbell on force, velocity, and perceived exertion during an acute session of high-intensity back squats in adults. Twelve recreationally trained men (23.0±2.6 years; 88.3±19.1 kg) randomly completed two sessions of 3 sets of 6 repetitions at the same absolute load using the OL barbell or SSB barbell. Force and velocity were measured on every repetition and rating of perceived exertion (RPE) was assessed for each set. A two-way ANOVA (set x barbell) with repeated measures and Sidak post-hoc test …

Gender Differences In Relations Between Social Comparison, Social Support, And Sleep Disturbance Among Midlife And Older Adults., Iris Bercovitz, Gabrielle M Salvatore, Jacqueline A Mogle, Danielle Arigo

College of Science & Mathematics Departmental Research

OBJECTIVE: To examine associations between sleep disturbance, social support, and social comparison among midlife and older adults, including the moderating role of gender.

METHODS: Adults ages ≥40 years (N = 557, MAge = 57, 53% men) completed a cross-sectional survey including validated measures of sleep disturbance, perceptions of social support, and social comparison orientation.

RESULTS: Sleep disturbance was negatively associated with social support (rs = −0.42 to–0.33, ps = 0.001) and associations were stronger for men than women–particularly perceived support from friends (η 2= 0.01). Sleep disturbance was also associated with upward comparison orientation (r = 0.12, p = 0.003), …

Thriving Transitions: Supporting Children With Autism Through Their Transition To School, Jennica Cano, Angela Blackwell

Summer 2024 OTD Capstone Symposium

Thriving Transitions is an educational guidebook created for parents of children with autism who are transitioning to school. Thriving transitions contains evidence and sensory based strategies for children with autism who are transitioning to school.

Knockdown Of Gonadotropin-Releasing Hormone Ii Receptor Impairs Ovulation Rate, Corpus Luteum Development, And Progesterone Production In Gilts, Amy T. Desaulniers, Rebecca Cederberg, Clay A. Lents, Brett White

Department of Animal Science: Faculty Publications

Reproduction is classically controlled by gonadotropin-releasing hormone (GnRH-I) and its receptor (GnRHR-I) within the brain. In pigs, a second form (GnRH-II) and its specific receptor (GnRHR-II) are also produced, with greater abundance in peripheral vs. central reproductive tissues. The binding of GnRH-II to GnRHR-II has been implicated in the autocrine/paracrine regulation of gonadal steroidogenesis rather than gonadotropin secretion. Blood samples were collected from transgenic gilts, with the ubiquitous knockdown of GnRHR-II (GnRHR-II KD; n = 8) and littermate controls (n = 7) at the onset of estrus (follicular) and 10 days later (luteal); serum concentrations of 16 steroid hormones …

Working Capital And Cash Flow Tools To Improve Farm Business Performance, Matthew Stockton

Cornhusker Economics

The point of this short essay is to briefly review the need and purpose of both tools, hoping that such a review will help farm managers and operators use them to reach their higher potential and improve their businesses and lives. Working capital represents the businesses’ ability to deal with short-term conditions and its resilience conferred upon it as a result of past performance in accumulating liquid assets. The cash flow document provides the ebb and flow of liquid assets during the normal cycle of doing business, providing recognizable events, plans that can be scrutinized, and identifiable challenges. These tools, …