Life Sciences Commons^™

The Impact Of Solvent Extraction And Enzyme-Assisted Extraction On The Yield And Quality Of Black Soldier Fly Larvae (Hermetia Illucens) Oil, Achini Madushani Mannawa Arachchige, Mark Williams, W. M.A.D.Binosha Fernando, Weeraya Karnpanit, Jane Lumanlan, Vijay Jayasena

Research outputs 2022 to 2026

The increased interest in black soldier fly larvae (BSFL) oil is driven by its beneficial fatty acid composition, leading to the development of effective extraction methods. This study evaluated the impact of solvent extraction (SE) and enzyme-assisted extraction (EAE) on the yield and quality of BSFL oil. SE using hexane, petroleum ether, isopropanol, and ethanol after 6 hr of extraction resulted in higher yields compared to EAE using alcalase, papain, and trypsin after 24 hr, with the highest yield recorded for petroleum ether (33%), followed by isopropanol (30%). Isopropanol extraction demonstrated the highest antioxidant activity (77%). The results indicated a …

Eggplant Variety Demonstration Results For West-Central Indiana, 2024, Petrus Langenhoven, Lizeth Sofia Catucuamba Tuquerrez

Midwest Vegetable Trial Reports

A non-replicated demonstration of eggplant varieties was conducted at Purdue University's Meigs Horticulture Facility to evaluate eight varieties, including four Italian-type and four Asian-type varieties, under Indiana growing conditions. Plants were transplanted on May 14, 2024, with 30 plants per variety in 180-foot rows. Weekly harvests occurred from weeks 8 to 18 after transplanting. Italian varieties consistently produced higher marketable yields than Asian types, with Nigral achieving the highest yield at 55,446 pounds per acre (17.7 fruits per plant, averaging 11.2 ounces per fruit). Rolandia produced the heaviest individual fruits, with an average weight of 12.3 ounces. Among Asian varieties, …

The Dirt Underneath My Fingernails: The American Pastoral And 21st-Century Food System Reform, Annabella Isaacson

Scripps Senior Theses

The following is an amalgamation of my experiences personally and vested interests academically, and is meant to be read as just that. As a thesis that combines Environmental Analysis and Writing & Rhetoric degrees, this project challenges traditional academic forms by integrating creative nonfiction and poetry with academic writing and analysis. This thesis attempts to fuse the academic and personal essay into one cohesive piece. The inconclusive nature of the narratives and explorations that follow are intended to represent my process in grappling with the histories and significance of American pastoral literature through the ways in which this genre has …

Assessing The Effects Of Wildfire Season Pm2.5 Exposure And Environmental Variables On Breeding Season Avian Body Condition, Cira Seyer-Ochi

Scripps Senior Theses

The increasing frequency and intensity of wildfires pose growing threats to wildlife, yet the indirect effects of wildfire smoke on avian populations remain largely unstudied. This exploratory study provides an initial assessment of how particulate matter 2.5 (PM2.5) associated with smoke pollution from the 2017 California wildfire season, in conjunction with precipitation and habitat type, influences the body condition of four resident bird species during the 2018 breeding season. Using bird banding data from 29 Monitoring Avian Productivity and Survivorship (MAPS) stations, I assessed the potential interactions between PM2.5 concentrations, precipitation, and habitat type with body condition for Song Sparrows …

We Found Love In A Wetter Climate: The Impact Of Varying Climatic Conditions On The Prevalence Of A Fungal Endophyte In Elymus Virginicus Populations, Audrey Yung

Scripps Senior Theses

Epichloë is a genus of endophytes– symbiotic fungi residing within a plant’s tissue– that can provide mutualistic benefits to its host. The stress gradient hypothesis predicts that mutualism between species becomes stronger in conditions of high abiotic stress. We tested this idea by assessing how Epichloë prevalence in Elymus virginicus populations is affected by varying climatic conditions. We established plots of E. virginicus with known endophyte frequencies in the spring of 2023 at five sites across a precipitation gradient in Texas and Louisiana. We collected E. virginicus recruits in June and July 2024 at all field sites. To determine recruits’ …

Behavioral Observations And Analysis Of Twix: The Cape Porcupine At The Lake Superior Zoo, Erin Davidson

Scripps Senior Theses

The three main goals of zoos are outlined as: education, conservation, and research. In our modern world, the focus of zoos has shifted away from simply housing animals, and towards how we can best promote proper animal welfare for individuals in human care. Animal welfare can be defined as the “mental health” of non-human species of fauna, and is greatly influenced by the stressors within the environment, as well as the option of choosing how to interact with and respond to the environment. Animals housed in zoos are presented with unique stressors that they often do not choose to experience, …

Characterization Of Dietary And Hormonal Influences On Renal Sodium Transporters And Channels In Rodent Models, Evelyn Chan

Scripps Senior Theses

Within kidneys, a diverse array of renal epithelial cells works to maintain electrolyte and water balance to regulate blood pressure and pH. These cells contain varying types of sodium transporters that adjust sodium reabsorption according to the body’s needs. Previous studies have observed sex-specific differences in renal sodium transporter regulation, which are thought to optimize reproduction in females. In this study, we performed high resolution microscopy and detailed characterization of cell type-specific expression and localization of major sodium transporters and channels in rodents in response to dietary and hormonal influences. Specifically, we examined the impact of dietary salt intake and …

If You Give A Tadpole A Chemosensory Stimulus: Detection Of Invasive Predatory Cues And Signs Of Conspecific Communication In Pseudacris Sierra Tadpoles, Faben Tesfazion

Scripps Senior Theses

Amphibians are at disproportionately higher risks of extinction than all other vertebrate classes, and a major causing factor is invasive predation. The American bullfrog (Lithobates catesbeianus), native to Eastern North America, is a notorious invasive species which preys on native Sierran treefrogs (Pseudacris sierra) in Northern and Central California. These treefrogs have yet to be placed on any Special Animals List, suggesting the presence of adopted behaviors against predation. Past studies have documented the importance of chemical sensing in anuran tadpoles as predator detection tools. This study tests if Sierran treefrog tadpoles can recognize invasive chemical …

El Maíz Del Abuelo: Fidel Márquez Sanchez’S Legacy And Mexico’S Historical Maize Agriculture, Lucia Marquez-Uppman

Scripps Senior Theses

The Green Revolution was an era of scientific development in productive agricultural methods and technologies in the mid-twentieth century. High-yielding seed varieties, artificial fertilizers, irrigation infrastructure, pesticides, and herbicides all developed from this period. Texcoco, Mexico was the center of much of the Green Revolution's education and research from the 1940s onward at the Autonomous University of Chapingo, a prestigious agricultural school, with funding from the US-based philanthropic Rockefeller Foundation. Powerful consequences in the globe's agriculture emerged from the Green Revolution, including the production of maize, a staple crop in Mexico, especially for Indigenous and campesino populations. My abuelo, Fidel …

Gain-Of-Function Tp53 Mutations Induce Non-Canonical Nf-Κb Transcriptional Targets To Promote Tumor Development, Kelsey A. Yutan

Scripps Senior Theses

Background: Gain-of-function TP53 mutations have previously been shown to induce chromosomal instability (CIN) in cancer cells, triggering the cGAS-STING innate immune response and subsequently activating the non-canonical NF-κB (NC-NF-κB) signaling pathway. This signaling cascade promotes cancer cell metastasis and an immunosuppressive tumor microenvironment (TME), significantly impacting tumor development and progression. However, the precise downstream mechanisms by which the mutp53-CIN-cGAS-STING-NC-NF-κB signaling pathway facilitates tumor development and progression remain poorly understood. Here, our objective was to identify direct downstream targets of mutp53-CIN-cGAS-STING-induced NC-NF-κB signaling that potentially impact the TME to promote the development and progression of oral squamous cell carcinoma.

Methods …

Commentary: Impact Of Resistance Training Intensity On Body Composition And Nutritional Intake Among College Women With Overweight And Obesity: A Cluster Randomized Controlled Trial, Joshua L Keller, Sarah E Deemer, Gian Luca Di Tanna, Sherri L Pals, Kevin R Fontaine, David B Allison

Children’s Nutrition Research Center Staff Publications

No abstract provided.

Characterization Of Actin Capping Protein Beta Isoform Specific Dynamics In Metastatic Cells, Etinosa Osamudiamen

All Graduate Theses, Dissertations, and Other Capstone Projects

To gain insight into the mechanism of cancer progression, we examined the isoform-specific roles of actin capping protein (CPβ1 and CPβ2) related to actin cytoskeletal dynamics and metastatic progression in human uterine epithelial cells (HeLa). We employed qPCR to determine the relative expression levels of CPβ1 and CPβ2 in actively growing HeLa cells. Our findings confirmed that CPβ1 and CPβ2 are differentially expressed, with CPβ2 being the predominant isoform, two-fold higher, consistent with its expression in other non-muscle tissues. To extend our expression studies, the localization and spatial distribution of the isoforms were visualized via immunolocalization studies using isoform-specific monoclonal …

Impact Of Obesity On The Gut Microbiome And Inflammatory Markers During Siv Infection And Antiretroviral Therapy, Casey M. Mcguire, Isaac R. Cinco, Diana Takahashi, Kristin A. Sauter, Melissa Kirigiti, Ilhem Messaoudi, Jonah B. Sacha, Charles T. Roberts, Paul Kievit

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Dysbiosis and impaired gut barrier integrity contribute to chronic immune activation associated with both obesity and HIV infection. Given the increased incidence of obesity in people living with HIV, we explored the impact of obesity on the gut microbiome and microbial translocation (MT) biomarkers during HIV infection and antiretroviral therapy (ART). Lean and obese rhesus macaques were infected with simian immunodeficiency virus (SIV) and subsequently treated with ART. Obese animals exhibited higher initial MT and inflammation biomarkers that remained constant throughout the study, while lean animals exhibited significant increases in these biomarkers that approached levels observed in obese animals. Lean …

Impact Of Bourbon Continuous Column Operations On Ethyl Carbamate Levels, Brad J. Berron, Jon Brown, Jason Gambrell, Travis Cantrell, Meghan Mcintosh, Sarah A. Wilson, Jarrad Gollihue, Harmonie M. Bettenhausen

James B. Beam Institute for Kentucky Spirits Faculty Publications

European and Canadian regulators enforce strict limits on the ethyl carbamate content of alcoholic beverages. North American distillers’ malt have high glycosidic nitrile levels which amplify the ethyl carbamate level of whiskey. Guidelines are required to produce low ethyl carbamate whiskey from North American distillers’ malts. This work challenges several strategies for reducing ethyl carbamate levels in bourbon and American whiskey products. New make spirit was produced using a bourbon mash bill on a continuous beer still with doubler. The low wines alcohol content and beer feed tray were systematically varied while the caustic cleaning schedules were monitored to generate …

‘Who’S Your Daddy?’: Ddradseq Parentage In Broodstock-Offspring Pairs Of Diadema Antillarum In A Population Restoration Initiative, Bianca R. Rosendahl

Undergraduate Scholarship and Creative Works

In the early 1980s Diadema antillarum, or the long-spined urchin, was hit by a catastrophic mass mortality event across the western Atlantic and Gulf of Mexico, inclusive of the Florida Keys. The cause of this die-off event was likely triggered by a pathogen or environmental stressor that decimated native populations by up to 98%. The species has not yet fully recovered and is struggling with recruitment success which is why it may benefit from an ex-situ recovery program. In this study, I used single nucleotide polymorphisms (SNPs) to identify genetic variation and analyze parentage in a captive population of …

An Understanding Of The Feeding Ecology Of Teleoceras Fossiger And Teleoceras Aepysoma Using Dental Microwear And Mesowear Analysis, Gabriel S. Goncalves

Master's Theses or Doctor of Nursing Practice

Teleoceras is a genus of rhinocerotids that belongs to the Tribe Teleoceratines and was widespread across North America from the late Early Miocene into the Early Pliocene. This genus was very successful, spreading across the continental United States from Florida and Tennessee to California and Washington state down to central Mexico and Honduras. Teleoceras is characterized by having a large, rounded chest cavity and short limbs with relatively high crowned teeth and lower incisors modified into tusk-like teeth. Initial ecological comparisons were made to the modern hippopotamus based on proportions of the chest cavity and its hypsodont dentition. Stable carbon …

Disruption Of Functional Network Development In Children With Prenatal Zika Virus Exposure Revealed By Resting-State Eeg, Ahmet Omurtag, Samah Abdulbaki, Thomas Thesen, Randall Waechter, Barbara Landon, Roberta Evans, Dennis Dlugos, Geetha Chari, A. Desiree Labeaud, Karen Blackmon

Brain and Mind Institute

Children born to mothers infected by Zika virus (ZIKV) during pregnancy are at increased risk of adverse neurodevelopmental outcomes including microcephaly, epilepsy, and neurocognitive deficits, collectively known as Congenital Zika Virus Syndrome. To study the impact of ZIKV on infant brain development, we collected resting-state electroencephalography (EEG) recordings from 28 normocephalic ZIKV-exposed children and 16 socio-demographically similar but unexposed children at 23–27 months of age. We assessed group differences in frequency band power and brain synchrony, as well as the relationship between these metrics and age. A significant difference (p < 0.05, Bonferroni corrected) in Inter-Site Phase Coherence was observed: median Pearson correlation coefficients were 0.15 in unexposed children and 0.07 in ZIKV-exposed children. Results showed that functional brain networks in the unexposed group were developing rapidly, in part by strengthening distal high-frequency and weakening proximal lower frequency connectivity, presumably reflecting normal synaptic growth, myelination and pruning. These maturation patterns were attenuated in the ZIKV-exposed group, suggesting that ZIKV exposure may contribute to neurodevelopmental vulnerabilities that can be detected and quantified by resting-state EEG.

Immune Checkpoint Inhibitors: Friend Or Foe, Antonio Cordova, Jesse Powell, Whitney Elg-Salsman, Caroline Mcculley

Books, Presentations, Posters, Etc.

Introduction: • Many cancers use mechanisms to escape killing by the immunesystem. • Cancer cells can mimicimmune checkpoint proteins such as cytotoxic T lymphocyte–associated antigen 4 (CTLA-4) ligand and the programmed death1 receptor ligand (PD-L1) toinhibit the T-cell response. • Immune checkpoint inhibitors (ICI's) are a novel set of monoclonalantibody cancer therapies. • ICI'sblockcheckpoint proteins from binding with their partner proteins oncancer cells thus allowing T-cells to kill tumor cells. • ICIs have promising anti-cancer results. • ICIs are also associated with immune related adverse events(irAEs), including dermatitis, pneumonitis, colitis,hepatitis, nephritis. • A rare but seriousirAEisICI-associated myositis causing bulbar and …

Impact Of Minimally Processed Red Meat On Biomarkers Of Cognitive Decline, Saba Vaezi

Electronic Theses and Dissertations

No abstract provided.

Evaluation Of High-Moisture Ear Corn As A Roughage Source In Finishing Diets Fed To Beef Steers, Riley J. Leeson

Electronic Theses and Dissertations

The objective of this study was to evaluate the feedlot performance, rumination activity, and carcass characteristics of high moisture ear corn (HMEC) as a roughage source in finishing diets fed to beef steers. Backgrounded steers (n = 191; initial shrunk weight [4% BW] = 449 kg  23.9 kg) were assigned randomly to 1 of 24 pens (8 steers/pen) with pens randomly assigned to one of four dietary treatments (6 pens/treatment): control diet with hay fed at 10% roughage equivalency (RE; HAY10), or HMEC at either 14% (EAR14), 10% (EAR10), or 6% (EAR6) RE. High-moisture ear corn was assumed to …

Finishing Location And Forage Diversity Influences Carcass Characteristics, Meat Quality Attributes, Terroir, And Color Stability Of Grass-Finished Bison Bulls, Garrett Tyler Weldy

Electronic Theses and Dissertations

The overall objective of this thesis is to determine the influence of finishing location and forage diversity on carcass characteristics, meat quality, terroir, and color stability of grass-finished bison bulls. The specific objectives were 1) Determine the effect of finishing location on carcass characteristics, meat quality attributes, and terroir of grass-finished bison bulls, 2) Investigate the effect of forage diversity on the carcass characteristics, meat quality attributes, and terroir of grass-finished bison bulls, and 3) Determine the effect of finishing location on the meat color stability of grass-finished bison bulls. Overall, results indicate that finishing location influenced carcass characteristics, objective …

The Influence Of Bottom-Up And Top-Down Effects On The Density And Distribution Of Common Leopards In The Eastern Himalayas, Karma Choki

Electronic Theses and Dissertations

Leopards (Panthera pardus fusca) are widely distributed across Asia, yet their conservation is overshadowed by the conservation status of other flagship predators like tigers (Panthera tigris), in the Eastern Himalayas. Despite their ability to coexist with other large carnivores, leopard populations are shaped by intricate ecological interactions and increasing human pressures. This thesis investigates the abundance and density, habitat use, and drivers of persistence of leopards across Bhutan to inform multi-species carnivore conservation planning. My first chapter provides an overview of the ecological roles of large carnivores and how they persist on the landscape. I review the mechanisms that facilitate …

Comparative Evaluation Of Fungal Strains For The Bioprocessing Of Soybean Hulls Using Cheese Whey As Fermentation Medium, Ruchita Bhattarai

Electronic Theses and Dissertations

Globally, soybean is the most widely cultivated oilseed crops, and serve as the primary source of cooking oil, contributing to around 50% of global oilseed production. During the industrial processing of soybean oil, a significant amount of soybean hull (SH) is produced, which makes up about 8% of the grain yield, resulting in approximately 18–20 million tons of hulls, one of the largest by-products in the soybean industry. The hulls have limited commercial value and are primarily used as fiber additives in dairy cattle feed, but a large portion is still discarded as agricultural waste. Similarly, cheese whey (CW), a …

Quantifying Landscape Variability Of Dynamic Soil Properties In The James River Valley, Eastern South Dakota, Sage Reuter-Schmitt

Electronic Theses and Dissertations

Dynamic soil properties (DSP) are a great tool for monitoring land-use change, and they are frequently used as soil health metrics. The NRCS and other soil researchers often use repeating, single-point or nested-point DSP studies to monitor and better understand the effects of land management decisions. While these studies capture the temporal variability of DSPs, they overlook the field-scale spatial variability of DSPs. Soil organic carbon (SOC) provides critical services to the ecosystem and other environments. These traits, as well as its susceptibility to management decisions, make SOC an important soil health indicator and DSP. Measuring SOC is often time …

The Spatial And Temporal Dimensions Of Plant–Soil Feedback In Native Perennial Monocultures, Bret Lang

Electronic Theses and Dissertations

Plant-soil feedbacks (PSFs) describe how plants change their soil environment in ways that can either negatively or positively affect subsequent plant growth. In doing so, they offer an opportunity to connect ecological processes with agricultural practice. My dissertation examines PSFs created by native perennial monocultures, commonly used for native seed production, across time and space, and through soil legacies. Specifically, I focus on how plant species identity shapes both soil nutrient and soil microbial community responses. To address these questions, I conducted field experiments with monocultures of five forb species native to South Dakota and established plots with these species …

Impacts Of Early/Mid-Gestation Shearing On Umbilical Blood Flow And Lamb Birth Weight, Mara Johnson

Electronic Theses and Dissertations

Studies outside the USA have reported that shearing pregnant ewes during mid to late gestation increases lamb birth weight. Our hypothesis was that shearing pregnant ewes on day 50 of gestation would increase umbilical blood flow (UBF), fetal size, lambing and placental weights. Twenty-eight Polypay ewes were randomly assigned to two treatments: shorn (S) and sham-shorn (Control, CON) and divided in singleton (S: n=4; CON: n=4), twins (S: n=5; CON: n=5) and triplets (S: n=5; CON: n=5). Umbilical BF and fetal and placental measurements were obtained via ultrasonography every 10 days from day 50 to 110 of gestation. Lamb birth …

Isolation And Characterization Of Rhizobia Bacteria From Midwest Soils, Jr Loya

Electronic Theses and Dissertations

Symbiotic bacteria, known as rhizobia, can colonize the roots of legume plants and then convert atmospheric nitrogen into a usable form by the host through the process called biological nitrogen fixation (BNF). Many of these bacteria produce other plant-growth-promoting benefits, such as nutrient acquisition, phytohormone production, and the modification of plant stress responses. Agricultural inoculants made from these bacteria have helped reduce the need for man-made nitrogenous fertilizers, known causes of pollution and eutrophication. There are additional benefits in developing bacteria inoculants local to regional soils. The ability to survive and compete in Midwest soil and climates present an opportunity …

Investigating Antimicrobial Efficacy Of Turmeric And Rosemary Oil Against Staphylococcus Aureus, Aishwarya Tripathi

Selected Full-Text Master Theses 2021-

This study investigates the antimicrobial efficacy of turmeric (Curcuma longa) and rosemary essential oil (Rosmarinus officinalis) against Streptococcus aureus, with a comparative analysis to phenoxyethanol, a widely used synthetic preservative. The growing consumer demand for natural cosmetic formulations have intensified the search for plant-derived alternatives to synthetic preservatives. Turmeric and rosemary oil were selected based on their documented antimicrobial, antioxidant, and anti-inflammatory properties. The objective of this research was to evaluate whether these natural agents can serve as effective preservatives in topical gel formulations, either individually or in combination, and how their efficacy compares to phenoxyethanol.

Eight gel-based formulations were …

Six Rivers National Forest Hazardous Fuels And Fire Management Project 2025 Orleans Complex Fire Effects From 9/13/25 - 9/14/25, James L. Parr, Nosheen Maung, Darby J. Lewis, Javier E. Margarito, Giancarlo Golloti

Environmental Science & Management Senior Capstones

The 2025 Orleans Complex affected the Klamath National Priority Landscape, designated by Congress as a priority to reduce fuel load and mitigate wildfire risk near rural, at-risk communities. To address the threat of large destructive wildfires, the 2023 Six Rivers Hazardous Fuels and Fire Management Project identified desired ecological conditions, using the right type of fire at the right time and place.

Exploring Topological Defects In Janus Bilayers Of Cr(I,Cl)3 And Cr(I,Br)3, Taksh Patel, Suyash Rijal, Charles Paillard, Changsong Xu, L. Bellaiche

2025 Research Poster Competition

Chromium halides such as CrI3 have attracted interest recently. They possess the remarkable property of sustaining long-range magnetic order down to the thickness of only a few layers [1, 2]. However, CrI3 lacks Dzyaloshinskyii– Moriya interaction (DMI) due to inversion center present between the adjacently bonded Chromium atoms.

The inversion center can be removed through the fabrication of Janus Monolayers by adding another trihalide along with Iodine to synthesize the material, that is to grow Janus monolayers Cr(I, X)₃ where X is another halide such as Cl or Br [3].

Previous work has predicted non-trivial spin textures such as …