Life Sciences Commons^™

Scientific Writing: A Guide From Data To Draft, Kristin M. Klucevsek

Open Education Materials

Textbook for ENGL302W (Scientific Writing)

CC BY-NC-SA (2025)

The Impact Of Diabetes Education & Post Discharge Interventions For African American Adults At Risk For & After Myocardial Infarction, Trinity L. Billingsley

Nursing | Student Research Posters

Cardiovascular disease or CVD refers to a multitude of conditions that affect both the heart and blood vessels. Being the leading cause of death in the United States, heart disease claims 919,032 lives; the risk of death becomes more likely when accompanied with comorbidities like diabetes (CDC, 2024). While heart disease is a leading cause of death across all populations, it accounts for a larger proportion of deaths in African American, or AA, adults at 22.6% compared to White adults at 18.0% and Hispanic adults at 11.9%, highlighting persistent racial disparities in cardiovascular outcomes (CDC, 2024). Diabetes as a comorbidity …

Systematic Review Of Immunohistochemistry Protocols, Arpitha Parthasarathy

Pitzer Senior Theses

Immunohistochemistry (IHC) is an essential technique in biological research that allows for the visualization and localization of proteins within tissue samples. This systematic review analyzes the most effective, efficient and reproducible protocols for IHC. It focuses on three critical steps: freezing, cryosectioning, and staining of skeletal muscle tissues in mice models. A total of 41, 44, and 48 articles on freezing, cryosectioning, and staining techniques, respectively, were analyzed, from reputable sources in biology like PubMed and Web of Science. Results showed the following. For freezing, the use of isopentane combined with liquid nitrogen appeared to be the best method, minimizing …

Crisis In The Current: A Proposed Study On The Impact Of Columbia River Basin Water Quality On Juvenile Chinook Salmon Survival, Olivia Buist-Thuillier

Pitzer Senior Theses

Salmon occupy a vital role in the ecosystem, economy, and indigenous culture of the Pacific Northwest of the United States, specifically within the Columbia River Basin. Of the five species of Pacific Salmon that live in the basin, Chinook salmon (Oncorhynchus tshawytscha) are the most widely commercially fished and are the largest. In recent years, Chinook Salmon populations have heavily declined from human influence. Rising temperatures from anthropogenic climate change, habitat destruction (dams, human presence), runoff from agricultural practices, and overfishing have all contributed to this decline. Because of their role as a keystone species and their cultural significance, there …

The Cognition Of Emotion: How Cognitive Control And Working Memory Underly Emotion Regulation Strategy Differences, Nathan Chabin

Pitzer Senior Theses

Working memory is crucial for comprehensively grasping the dynamics of emotion regulation (ER) and cognitive control, as it serves as a foundational cognitive resource that influences how individuals manage their thoughts, emotions, and behaviors. This research aimed to examine the role working memory capacity (WMC) has on Frontal Midline Theta (FMT), an EEG marker for cognitive control, during two common ER strategies: emotional suppression and cognitive reappraisal. Participants performed both an Operation Span working memory task and an ER task, where they viewed a series of negative and neutral images across three conditions. First, participants were instructed to passively view, …

Comparative Tendons, Frank E. Fish

Biology Data Repository

The data are for examination of the physical properties of the tendons in the peduncle of the harbor porpoise to compare the dorsal and ventral tendons. Data are from mechanical testing of tendon fascicles by ramp to failure, stress relaxation, and crimp tests.

Final Comparison Of Blue & Humpback Forms, Frank E. Fish

Biology Data Repository

No abstract provided.

Impact Of Hiv-2 Rod9 Vpx Mutations On Samhd1 Degradation And Vpx Nuclear Trafficking, Tolulope R. Oladimeji

All Graduate Theses, Dissertations, and Other Capstone Projects

Human Immunodeficiency Virus (HIV) is a leading cause of death in many parts of the world. HIV-2, endemic to West Africa, has infected over 2 million people globally. A key difference between HIV-2 and the more widespread HIV-1 is the HIV-2 protein Vpx, which facilitates infection in non-dividing cells by targeting SAMHD1, a host innate immune protein, for proteasomal degradation. This research aims to identify specific amino acid residues in HIV-2 ROD9 Vpx that are essential for mediating SAMHD1 degradation by the proteosome. I hypothesize that amino acid residues located in the C-terminal region of Vpx play a critical role …

Chronic Alcohol Consumption Induces Phenotypic And Functional Alterations Consistent With A Hyper-Inflammatory State In Peripheral Blood Mononuclear Cell-Derived Microglia In A Rhesus Macaque Model, Hami Hemati, Madison B. Blanton, Heather E. True, Jude Koura, Rupak Khadka, Kathleen A. Grant, Ilhem Messaoudi

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Alcohol-induced dysregulation of microglial activity is associated with neuroinflammation, cognitive decline, heightened risk for neurodegenerative diseases, alcohol dependence, and escalation of alcohol drinking. Given the challenge of longitudinally sampling primary microglia, we optimized an in vitro method to differentiate peripheral blood mononuclear cells (PBMC) from rhesus macaque (RM) into induced microglia-like cells (RM-iMGLs). The RM-iMGLs displayed transcriptional profiles distinct from monocyte progenitors and closely resembling primary microglia. Notably, morphological features showed that differentiated RM-iMGLs derived from subjects with chronic alcohol consumption (CAC), while bigger, exhibited a bipolar-like morphology. Additionally, dysregulation in key inflammatory and regulatory markers, along with increased baseline …

Upper And Lower Body Volume-Equated Resistance Exercise Increases Post-Exercise Lipid Oxidation In Trained Eumenorrheic Females., Alyssa L. Parten, Abbie E. Smith-Ryan, Eric K. O'Neal, T Scott Lyons, Hunter S. Waldman

Theses

Exercise strategies targeted to increase lipid oxidation (LOx) in the resting state present an opportunity to optimize long-term metabolic health. However, research examining the effects of resistance training (RT) on LOx in females remains scarce, particularly with no studies yet examining whether differences exist in LOx between upper and lower body RT. This study examined LOx changes following volume-load equated upper and lower body RT in well-trained, eumenorrheic females. Twenty-two (age: 25.4 ± 5.6 years, BMI: 24.6 ± 2.7) females completed a 3-repetition-maximum (RM) and 1RM test for the barbell back squat (BS) and bench press (BP), respectively. From these …

Restoring Rattlesnake Creek: Using Citizen Science To Assess The Ecological And Morphological Impacts Of Dam Removal In Western Montana, Bethany Blakey

Undergraduate Theses, Professional Papers, and Capstone Artifacts

As dam removals increase in frequency across the U.S., most research has focused on the impact of larger dam removals, despite the removal of small dams being much more common. Given the hundreds of small dams in Montana, this research uses citizen science data collected over eight years spanning before and after a 2020 small dam removal in Rattlesnake Creek, Montana, to investigate impact on stream ecology and morphology. We analyzed pebble count grain size distributions and aquatic macroinvertebrate biotic indices from 2017 to 2024 to assess changes in sediment transport and macroinvertebrate population as well as evaluate the efficacy …

Inquiries Into The Mycobacterial Caseinolytic Protease System, Felipe Badke D'Andrea

Student Theses and Dissertations

Despite progress over the last century, Mycobacterium tuberculosis (Mtb), the causative agent of tuberculosis (TB), remains the leading cause of death from an infectious disease worldwide. The high global burden of Mtb, alongside its protracted treatment regimen and insidious rise of multi-drug resistance, underscores the need for non-canonical drug targets and pathways to improve antitubercular chemotherapy. Mtb's success as a pathogen is linked to its ability to infect and evade clearance by the human immune system despite a wide range of noxious host stressors. Along with transcriptional responses, the maintenance of protein homeostasis is critical to this adaptability and pathogenicity. …

Nurture Is Nature: The Molecular Basis Of Caregiver Genotype Effects On Larval Growth And Caste Development In The Clonal Raider Ant, Patrick Konrad Piekarski

Student Theses and Dissertations

The distinction between nature (genetics) and nurture (environment) is blurry, in part because the environment includes the genes of social partners. This is especially evident for parents and their offspring, where genes expressed in one partner profoundly influence the physiology and behavior of the other. Such indirect genetic effects (IGEs) are mediated through social interactions. Although parental care has evolved in many disparate lineages, in most systems it is challenging to delineate the effects of genetics and environment on the phenotypes of caregivers and offspring. This is because most animals with parental care reproduce sexually, meaning each genotype is represented …

Serine Metabolism Leverages The Integrated Stress Response To Direct Stem Cell Fate During Tissue Regeneration, Jesse Stephen Swyer Novak

Student Theses and Dissertations

Epidermal stem cells constantly rejuvenate the skin's barrier, which excludes harmful microbes and prevents dehydration. While hair follicle stem cells (HFSCs) normally regenerate hair, they must also reconstruct and thereafter maintain the overlying epidermis upon a barrier breach, such as a wound. How these fate choices are balanced to restore physiologic function to damaged tissue remains poorly understood. Here, I uncover the non-essential amino acid serine as a surprising regulator in this process. Utilizing dietary intervention and HFSC-specific loss-of-function studies, I demonstrate that under serine-depleted conditions HFSCs delay hair growth. Upon injury, serine-restricted HFSCs skew their fate towards epidermal re-epithelialization …

Molecular And Cellular Mechanisms Of Lim Protein Mechanotransduction, Donovan Yong Zhi Phua

Student Theses and Dissertations

For tissues to develop and maintain mechanical homeostasis, cells must be able to perceive and respond to mechanical cues in their local environments (mechanosense). While there has been significant progress in understanding the physiological significance of mechanosensation, the mechanisms by which proteins convert mechanical stimuli into biochemical signals (mechanotransduction) are poorly understood. The actin cytoskeleton serves as a nexus for cellular mechanotransduction, generating and transducing forces into intracellular signals that coordinate cell migration, cellular contractility, organelle dynamics, and gene expression. However, our understanding of the molecular mechanisms by which cytoskeletal mechanotransduction is achieved is limited. LIM domain proteins are a …

Quantification Of Auxin-Cytokinin Response During Primary Root And Lateral Root Development In Common Bean, Gurdeep Singh

Electronic Theses and Dissertations

Roots support water and nutrient uptake, anchorage, and soil interactions through continuous growth at the primary root tip (PRT). In common bean (Phaseolus vulgaris), new cells are produced in an open root apical meristem and subsequently elongate in the elongation zone, and together these processes drive root tip progression through the soil. Lateral roots (LRs) arise in the differentiation zone, where pairs of pericycle founder cells dedifferentiate, divide asymmetrically, and form lateral root primordia (LRP) that pass through defined developmental stages before emergence. Auxin and cytokinin (CK) are central regulators of both primary root growth and LR development, yet their …

Electrical Homeostasis Of The Inner Mitochondrial Membrane Potential, Peyman Fahimi, Lázaro A. M. Castanedo, P. Thomas Vernier, Cherif F. Matta

Bioelectrics Publications

The electric potential across the inner mitochondrial membrane must be maintained within certain bounds for the proper functioning of the cell. A feedback control mechanism for the homeostasis of this membrane potential is proposed whereby an increase in the electric field decreases the rate-limiting steps of the electron transport chain (ETC). An increase in trans-membrane electric field limits the rate of proton pumping to the inter-membrane gap by slowing the ETC reactions and by intrinsically induced electroporation that depolarizes the inner membrane. The proposed feedback mechanism is akin to a Le Chatelier's-type principle of trans-membrane potential feedback control.

How Forest Stand Conditions Influence Whitebark Pine And Clark’S Nutcracker: A Systematic Review, Bryan Mathew Cohen

Undergraduate Theses, Professional Papers, and Capstone Artifacts

Whitebark pine (Pinus albicaulis), a keystone species of high-elevation ecosystems of western North America, is threatened with extinction due to disease (Blister rust), pests (Mountain pine beetle), and climate change. Regeneration of the species depends nearly exclusively on the seed-caching behavior of Clark’s nutcracker (Nucifraga columbiana, Family Corvidae). Because of this, conservation of whitebark ecosystems requires restoring stand structures that favor nutcrackers. Although there are increasing numbers of studies on the relationship between nutcrackers and whitebark pine, findings have not yet been synthesized, limiting knowledge for application to management. I did a systematic literature review to answer the following questions: …

Exploring The Role Of Bingocize® Workshops In Enhancing Health And Well-Being In Older Adults, Kaitlyn Gray

Mahurin Honors College Capstone Experience/Thesis Projects

As the population of older adults grows, the need for better health and well-being also grows, making it necessary to implement effective strategies for healthy aging to improve the length and quality of life of older adults (CDC, 2024). This study evaluates the effectiveness of a Bingocize^® Workshop in improving the physical health of older adults. Bingocize^®, a unique program combining bingo with exercise, is designed to increase physical activity. Eleven (11) participants aged 68.55 ± 6.235 attended a ten-week workshop, two times per week focusing on physical exercises tailored to their abilities. The primary objective was …

Improving Glucose Levels In Adults With Type 2 Diabetes, Kaitlin Castillo

Doctor of Nursing Practice (DNP) Scholarly Projects - Archive

Type 2 diabetes is a growing health crisis, posing significant challenges not only to individual well-being but also to healthcare systems' financial sustainability. The prevalence of type 2 diabetes continues to rise annually, contributing to increased medical costs, hospitalizations, and long-term complications. Houston, Texas, has a diabetes prevalence of 13.5%, exceeding the national average of 10%. A clinical gap exists in providers’ ability to prevent and manage type 2 diabetes. Prioritizing prevention, early intervention, and improved patient monitoring can reduce incidence rates, lower healthcare costs, and enhance overall population health. A Plan Do Study Act model was utilized for the …

Investigating The Role Of Fnci-1 In The Development And Function Of The C. Elegans Nervous System, Meagan Collins

Open Access Master's Theses

Fanconi Anemia (FA) is a rare human genetic disease caused by mutation of any of twenty-three genes on the FA pathway, which functions in the repair of DNA damage and the maintenance of genome stability. FA is clinically characterized by congenital abnormalities and a predisposition to aplastic anemia and malignancies. Recently, progressive neurological symptoms and abnormal neurological findings on imaging have been noted in FA patients, including cerebral and cerebellar lesions, calcification, necrosis, gliosis, and associated acute and chronic neurological symptoms; this constellation of symptoms has been termed Fanconi Anemia-Associated Neurological Syndrome (FANS).

To elucidate the molecular etiology of FANS, …

Association Between Vitamin D Status And Cortisol Concentration Among Pregnant Women, Kenneth Addae

Open Access Master's Theses

Objective: Vitamin D deficiency (VDD) and high circulating cortisol during pregnancy have each been linked to adverse maternal and child outcomes such as preterm birth. However, limited research has explored the association between vitamin D status and cortisol concentration during pregnancy. Our objective for this study was to determine the association between vitamin D status and cortisol concentration among pregnant women.

Methods: The Prenatal Health and Nutrition (PHAN) study was a pilot cross-sectional study of 30 healthy pregnant women conducted in targeted areas in Rhode Island. Data was collected on sociodemographic characteristics, substance use, perceived stress scale, household food security, …

The Effects Of Shoe Forefoot Design On Center Of Pressure And Knee Moments During Running In A Fatigued And Non-Fatigued State, Tyler Tran

Open Access Master's Theses

Runners experience high injury prevalence, which can be attributed to the overloading of the musculoskeletal system during the repetitive motion of running (Kakouris et al. 2021, Lopes et al. 2012). While some running mechanics are associated with better performance or increased risk for developing an orthopedic condition, many are still poorly understood (Van Hooren et al. 2024, Willwacher et al. 2022). Center of pressure and frontal plane knee moments are two such mechanics with a limited body of evidence leading to its importance (Mehdizadeh et al 2021, Baker et al. 2018). Although running mechanics describe the motion, the origin of …

Diet Quality And Quality Of Life Among People With Parkinson’S Disease, Elizabeth Tierney

Open Access Master's Theses

Poor diet quality (DQ) and reduced quality of life (QoL) are concerns for people with Parkinson’s disease (PwPD). Non-motor (NMS) and motor symptoms (MS) significantly impact the DQ and QoL of PwPD which can be alleviated through diet intervention. This study examined the relationship between DQ and QoL in PwPD and investigated participant perspectives to identify themes related to nutrition care. This was a secondary data analysis of EMPOWER PD. Diet intake was assessed via the Dietary Screening Tool (DST). Quality of life was assessed by the Parkinson’s Disease Questionnaire-39-item (PDQ-39). Participant perspectives were collected through 1:1 interviews. Nineteen participants …

Herbivore Kairomones Affect Germination Speed, Seedling Growth, And Herbivory, Brooke Pellegrini

Open Access Master's Theses

Seeds and seedlings are particularly vulnerable to herbivory. Unlike mature plants, which can wait until herbivory is experienced to induce defense, seeds and seedlings face mortality if they wait. Slug mucus functions as a kairomone, a non-attack-related substance emitted by consumers that is detected by a prey species (in this case, plants). While snail mucus has been shown to induce defense in seedlings, it is not widely confirmed whether slugs have the same effect and whether seeds can also detect and react to such herbivore cues. We investigated how exposure to Arion subfuscus mucus affected growth and defense in Brassica …

Field Performance Evaluation Of Selectively Bred Eastern Oyster Lines In New Jersey And Rhode Island, Seraphina Satkowski

Open Access Master's Theses

Eastern oyster aquaculture has increased steadily over the last three decades, but the industry has yet to achieve its full potential. In the Northeast USA, production is limited in part by a lack of high performing stocks suited to the geographically broad and heterogenous growing environments. For this study, hatchery lines from Maine, New York, and New Jersey as well as crosses between each hatchery line and the selectively bred Northeast high survival (NEHÒ) line, were deployed at oyster farms in Rhode Island and New Jersey for performance evaluation. Replicate bags of each line were stocked and maintained according to …

Evaluating Yield And Quality Of Heat-Tolerant Broccoli Varieties For Rhode Island Farmers, Calla Pederson

Open Access Master's Theses

In the United States, broccoli is a popular vegetable, important for its nutrition and economic contributions alike. However, its future production is at risk as broccoli prefers cooler climates, with an optimal temperature range of 15 - 23°C (59 - 73°F) for growth, especially in the weeks leading up to harvest. Temperatures above this threshold lead to failure to develop, damage, and unmarketability. There has also been an emphasis on bringing broccoli production back to the East coast from California, as climate events threatened Californian agriculture and local food has become popular, necessitating outside of season broccoli production during times …

Investigation Of Csld And Pme Genes In Cell Division And Expansion In Physcomitrium Patens, Lia Bozza

Open Access Master's Theses

Cellulose Synthase-Like Ds (CSLDs) are cellulose synthase known to participate in cell plate formation and cell wall remodeling. In the moss model, Physcomitrium patens, double csld2/6 knockout (KO) mutants were previously shown to have gametophore leaf phenotypes that are consistent with defects in cytokinesis, including irregular cell shape, cell separations, and incomplete midrib formation (Wu et al., 2023a). When these mutants were grown on ammonium-free medium to promote gametophore development, the severity of the phenotype was more extreme, including incompletely formed cell plates and multinucleated cells (A. Roberts, personal communication). It was also reported that expression of several enzymes …

Investigating Regulation And The Regulome Of The Nucleic Acid Binding Protein Spovg Within Borrelia Burgdorferi, Timothy C. Saylor

Theses and Dissertations--Medical Sciences

Prior studies indicated Borrelia burgdorferi SpoVG protein was regulated, SpoVG binds specifically to a number of nucleic acids, and SpoVG binds to the non-variable section of the major virulence factor vlsE. Although a direct transcriptional activator or inhibitor was not elucidated, it is shown that SpoVG is regulated based on cell culture density independent of the growth rate of B. burgdorferi, indicating that SpoVG regulation is outside the scope of the alternative sigma factor RpoS. Using a dCas9 CRISPRi construct to target SpoVG resulted in protein expression trending towards upregulation of the BosR-RpoS regulon, and a marked decrease …

Leveraging Clinical Standards To Improve The Translational Relevance Of Therapies For Experimental Spinal Cord Injury, Aaron L. Silverstein

Theses and Dissertations--Neuroscience

Efforts to improve treatment of spinal cord injury (SCI) often use animals to develop effective therapeutics, intending to improve the survival and the quality of life of persons with SCI. Over half of all SCIs in the United States occur at the cervical level and often cause locomotor deficits and life-threatening breathing dysfunction. Thus, my dissertation is focused on this important patient population.

After beginning with a description of translational research and surveying SCI from a clinical standpoint, I turned to describe experimental models of cervical SCI and more detailed neuroimmunological findings relevant to my work. Then, I conducted a …