Life Sciences Commons^™

Blood Biomarkers In Down Syndrome: Facilitating Alzheimer’S Disease Detection And Monitoring, Melissa E. Petersen, Lisi Flores-Aguilar, Elizabeth Head, Laia Montoliu-Gaya, Andre Strydom, Sarah E. Pape, Juan Fortea, Nicholas J. Ashton, Chinedu Udeh-Momoh, Sid E. O’Bryant, Dwight German, Florin Despa, Mark Mapstone, Henrik Zetterberg

Neurology Faculty Publications

Blood-based biomarkers continue to be explored for disease detection, monitoring of progression, and therapeutic outcomes as the diagnostic determination of Alzheimer’s Disease in Down Syndrome (DS-AD) remains challenging in clinical settings. This perspective highlights the current status of this effort. Overall, amyloid (A), tau (T), and neurodegeneration (AT[N]) blood-based biomarkers have been shown to increase with disease pathology for individuals with DS. Phosphorylated tau biomarkers (p-tau217, p-tau181) have been consistently shown to track disease progression for DS-AD and are likely good candidates for use in clinical settings. Biomarkers of inflammation (glial fibrillary acidic protein) also show promise; however, additional work …

Effects Of Dietary Energy Source And Stress On Equine Intestinal Permeability And Inflammation, Amanda R. Bauman

Theses and Dissertations--Animal and Food Sciences

Dietary concentrate is often provided to exercising horses to meet energy requirements. However, both high-starch and high-fat diets may be associated with alterations in intestinal barrier function. To investigate this, 32 mature horses were fed one of four isocaloric dietary concentrate treatments (n = 8 per treatment): 1) a concentrate with cracked corn providing 4 g starch⋅kg BW^-1⋅d^-1 and 0.2 g fat⋅kg BW^-1⋅d^-1 (CC), 2) a concentrate with puffed corn providing 4 g starch⋅kg BW^-1⋅d^-1 and 0.2 g fat⋅kg BW^-1⋅d^-1 (PC), 3) a concentrate with puffed corn and …

Metabolic And Barrier Function In Response To Β-Caryophyllene In Intestinal Epithelial Cells, Hannah Scroggins

Theses and Dissertations--Animal and Food Sciences

Butyrate, a short-chain fatty acid, regulates cellular metabolism and energy status in intestinal epithelial cells. β-caryophyllene (BCP) is a plant-derived compound that may provide advantageous effects on intestinal barrier function and nutrient utilization. The goal of this thesis was to investigate the effects of BCP on butyrate utilization and metabolism in intestinal epithelial cells. Throughout this thesis, Caco-2 cells in hanging inserts in multi-well plates were used as a model for the intestinal barrier. One study was conducted to investigate the effect of BCP on butyrate utilization and barrier function in intestinal epithelial cells. A second study aimed to elucidate …

Comparison Of Sheep And Cattle Rumen Microbiological And Physiological Responses To An Abrupt Transition From A High-Forage To A High-Concentrate Diet, Katherine Purvis

Theses and Dissertations--Animal and Food Sciences

Sheep and cattle are often used interchangeably due to having the same gastrointestinal anatomy; however literature directly comparing the two species is minimal. Previous in vitro studies have pointed towards potential species differences in rumen carbohydrate utilization and acidosis susceptibility. The objective of this study was to evaluate in vivo microbiological and physiological responses of wethers and steers when abruptly transitioned from a high-forage to a high-concentrate diet. We hypothesized there are differences between species in response to abrupt dietary transition with respect to intake, rumen microbiology, and the rumen environment. Individually housed, rumen cannulated purebred Polypay wethers (n = …

Characterization Of P38alpha And Nitric Oxide Synthase 3 Binding, Gillyan M. Jewett

Master's Theses

P38alpha is a member of the mitogen activated protein kinase (MAPK) family. This family is important for mediating signal transduction pathways through phosphorylation of downstream substrates. Previous work has described the ability of p38alpha to bind and phosphorylate nitric oxide synthase 3 (NOS3) at two sites, the autoinhibitory loop (AI) at S600 and the N-terminal MAPK binding site (NtMAP) at S114. P38alpha has two substrate binding domains, the common docking groove (CD-groove) and a hydrophobic pocket (DEF) that are important in binding and phosphorylating various substrates specific to each pocket. Utilizing biolayer interferometry, this work describes the importance of both …

New Observations Of Common Snook, Centropomus Undecimalis, In Mississippi Coastal Waters, With A Summary Of Local Records, Tiffany A. Weidner, William C. Tilley, Brooke Doucet, Angelos Apeitos, Eric A. Saillant, James S. Franks, Jeremy M. Higgs

Gulf and Caribbean Research

No abstract provided.

The Macroalgal Composition Differs Among Depths And Zones In Cuban Mesophotic Coral Reef Eosystems, Beatriz Martínez-Daranas, Dennis Hanisak, Partricia M. González-Sánchez, Stephanie Farrington, John K. Reed

Gulf and Caribbean Research

Previously the algal community of Cuban mesophotic coral reef ecosystems (MCEs) has not been characterized quantitatively. The objective of this study was to explore the distribution of macroalgae around Cuba and their depth profiles within the mesophotic zone (30−150 m). Data on the algal community were obtained in 2017 during 43 ROV (remotely operated vehicle) dives around Cuba’s shelf. Scientists specializing in algal taxonomy watched the live ROV video on board the ship and recorded algal presence every ~5—10 min in a database. Algae were identified to the lowest possible taxa, and algal presence/absence was analyzed in 20 m depth …

Early Season Tropical Cyclones Affect Birds Breeding On A Barrier Island, Theodore J. Zenzal Jr., Amanda N. Anderson, Brock Geary, Jessica Schulz, Robert C. Dobbs, Wylie C. Barrow Jr., James H. Waddle

Gulf and Caribbean Research

Animal populations often experience acute natural disturbances, most of which are connected to short—term weather events. Occurrences of early—season tropical cyclones during the peak of the avian breeding season are likely to increase with climate change, which can substantially impact populations of coastal breeding birds at multiple scales. To understand the acute impacts of severe tropical cyclones, we investigated how abundances of breeding birds changed before and after 4 early season named tropical cyclones on a barrier island in the Gulf of Mexico. We detected a change in pre— versus post—storm numbers of breeding birds after 2 out of 4 …

Presence Of Potential Nursery Habitat For The Lemon Shark In Mississippi Coastal Waters, Lindsay K. Bomgardner, P. O. Grammer, Angie Hoover, Anna Millender, Jeremy Higgs, Jill M. Hendon, Michael J. Andres

Gulf and Caribbean Research

No abstract provided.

Ina Scuba: Mobilization Of French West Indies Scuba Diving Clubs To Rapidly Improve Knowledge On Threatened Marine Species. Case Of The White-Spotted Eagle Ray (Aetobatus Narinari)., Océane Beaufort, Rocio Prieto González

Gulf and Caribbean Research

The White—Spotted Eagle Ray (Aetobatus narinari), listed as Endangered on the IUCN Red List since 2021, is experiencing alarming population declines, underscoring the urgent need for targeted conservation efforts. This species, which plays a vital ecological role in marine food webs, has biological and ecological characteristics that make it particularly vulnerable to habitat degradation and anthropogenic pressures, particularly fishing, tourism, and maritime traffic. In the French West Indies (FWI), the paucity of data on the White—Spotted Eagle Ray remains a major obstacle to the development of effective, island—specific conservation strategies. To address this gap, the INA Scuba participatory …

Oyster Horizons: Spatial Prioritization For Oyster Restoration At Alabama Living Shorelines, Trinity Henry-Jack, Aaron Bland, Christa Russell, Ronald Baker

Gulf and Caribbean Research

No abstract provided.

Integrating Phytochemicals And In Silico Methods For Modern Drug Discovery: A Comprehensive Review, Olorunfemi Oyewole Babalola, Kpomah Bridget, Godspower Oyubu, Sakariyau Adio Waheed, Samuel Ayomikun Ajiboye, Aderonke Elizabeth Fakayode, Adeleye Adegboyega Edema, Udensi Great Chukwuma, Rachael Taiwo Tiamiyu, Adebisola Joy Fakayode, Hameedah Oluwatoyin Adebimpe, Kosisochukwu Dematus Onyeagba, Comfort Oluseun Fakunle, Paul Olamide Ottu, Sonia Gabriel

Chemistry & Biochemistry Faculty Publications

Phytochemicals have long played a central role in drug discovery due to their structural diversity and broad biological activity. This review explores the progression of natural product-based drug development, highlighting how traditional knowledge and modern scientific tools are increasingly being combined. Medicinal plants identified through ethnopharmacological practices continue to guide the search for bioactive compounds, while advancements in screening techniques and structural analysis have improved the isolation and characterization of these substances. The review discusses the wide range of pharmacological actions found in plant-derived compounds. Their mechanisms of action and potential for synergistic effects are examined in detail. The application …

Phytochemical And Nutritional Composition Of Vernonia Amygdalina: Antioxidant, Antidiabetic Efficacy, And Hematological Benefits, Hassan A. Adewuyi, Messiah Atapia, Emmanuel Ochonung, Sakariyau A. Waheed, Adebimpe H. Oluwatoyin, Chizoba V. Obunadike, Adeleye A. Edema, Samad Hussein, Usman A. Mohammed, Kevin L. Sebastine, Tolulope O. Jaiyeola

Chemistry & Biochemistry Faculty Publications

Vernonia amygdalina, a traditionally used medicinal plant, has been purported to possess health-promoting properties. The aim of this study is to investigate the phytochemical and nutritional composition of V. amygdalina, evaluate its antioxidant and antidiabetic efficacy, and assess its hematological benefits. Phytochemical screening, proximate analysis, antioxidant assays (DPPH, ABTS), antidiabetic evaluations in treptozotocin induced diabetic rats, and hematological assessments were conducted according to standard protocol. V. amygdalina exhibited a rich phytochemical profile, comprising flavonoids, phenolic acids, and terpenoids, alongside significant antioxidant activity (IC50 = 12.5 μg/mL) and complete source of protein. Proximate analysis revealed high protein (21.1%) and …

Bacterial Two-Hybrid Systems Evolved: Innovations For Protein-Protein Interaction Research, Rebecca M. Richardson, Steven M. Pascal

Chemistry & Biochemistry Faculty Publications

Bacterial two-hybrid (B2H) systems offer a versatile platform for detecting protein-protein interactions (PPIs) in vivo. Originally developed to study bacterial transcriptional regulators, these systems have evolved to support a wide range of applications, including interaction mapping, domain analysis, and high-throughput screening. This review surveys the development and expansion of B2H systems across diverse biological contexts. We highlight technical considerations, platform-specific innovations, and recent adaptations that extend the utility of B2H systems in both prokaryotic and eukaryotic protein interaction research environments. By comparing different B2H variants and discussing their integration with complementary approaches, we provide a framework for leveraging these …

Clostridioides Difficile Infection Study Models And Prospectives For Probing The Microbe-Host Interface, Tatiana Zvonareva, David S. Courson, Erin B. Purcell

Chemistry & Biochemistry Faculty Publications

Clostridioides difficile infection (CDI) is an urgent public health threat with a high rate of recurrence and limited treatment options. In vivo models have been indispensable in understanding CDI pathophysiology and establishing treatment protocols and continue to be essential in pre-clinal testing. More importantly, in vivo models offer the opportunity to probe the complex systemic host response to the microbe, which is impossible to recapitulate in vitro. Nonetheless, constraints related to the availability of animal models, cost, ethical considerations, and regulatory control limit their accessibility for basic research. Furthermore, physiological and habitual divergences between animal models and humans often result …

Lap-Hsp60 Complex Modulates Epithelial Tight Junction Barrier, Arun Bhunia, Manalee Manalee Samaddar, Chen Sun, Nicholas Gallina, Nicole Irizarry-Tardi, Donqi Liu, Shivendra Tenguria, Rishi Drolia, Anika Jain, Daisuke Kihara, Wen Jiang, Kee-Hong Kim, Abigail Cox, Kurt Ristroph, Gregory Knipp, Nicholas Noinaj

Biological Sciences Faculty Publications

During infection, Listeria adhesion protein (LAP), a housekeeping enzyme, acts as a tight junction modulator (TJM) through interaction with Hsp60 to facilitate Listeria monocytogenes translocation across the intestinal epithelial barrier. Here, we used purified LAP as a potential TJM to overcome the limiting and variable effects observed by other agents in the class. We structurally determined the LAP interaction alone and in complex with Hsp60 utilizing cryo-EM and computational analysis. LAP structure resolved at 2.83 Å, forms multimeric interlocking dimers and tetramers, and the N-domain interacts with Hsp60, while the C-domain bridges the bacterial surface receptor InlB. The structural studies …

Alternative Splicing In A Coral During Heat Stress Acclimation And Recovery, Nitin Baliga, Kathryn Stanklewicz, Jacob Valenzuela, Serdar Turkarsian, Wei-Ju Wu, Kelly Gomez-Campo, Nicolas Locatelli, Trinity Conn, Veronica Radice, Katherine Parker, Rachel Alderdice, Line Bay, Christian Voolstra, Daniel Barshis, Iliana Baums

Biological Sciences Faculty Publications

Climate change has caused drastic declines in corals. As sessile organisms, corals acclimate to environmental shifts through genome-wide changes in gene expression, epigenetic modifications, and alterations in microbiome composition. However, alternative splicing (AS), a conserved mechanism of stress response in many organisms, has been under-explored in corals. Using short-term acute thermal stress assays, we investigated patterns of AS in the scleractinian coral Acropora cervicornis during response to low (33°C), medium (35°C), and high (37°C) heat stress and subsequent overnight recovery. Our findings demonstrate reproducible dynamic shifts in AS of at least 40 percent of all genes during response to heat …

Selective Nutrient Incorporation May Underestimate Heterotrophy Of A Mixotrophic Reef-Building Coral, Connor R. Love, Marleen Stuhr, Michael D. Fox, Veronica Z. Radice, Maoz Fine, Kelton W. Mcmahon, David L. Valentine

Biological Sciences Faculty Publications

Mixotrophic reef-building corals acquire nutrition via photosynthate translocation from endosymbiotic microalgae and by heterotrophic prey/particle capture. Heterotrophy promotes resistance to, and recovery from, environmental stress, but quantifying coral heterotrophy remains difficult due to complex resource exchanges within the coral holobiont. We interrogated the response of multiple biomarkers to coral heterotrophy using fatty acid profiling and stable isotope analysis of Stylophora pistillata grown along a controlled feeding gradient from pure autotrophy to pure heterotrophy. We found that fatty acids and nitrogen were effectively incorporated into both coral host and symbiont tissues, while carbon, which is the primary target for conventional heterotrophy …

Huchum: The Legacy Of Fire Suppression, Indigenous Land Management, And Media Representation, Carolyn Coyne

Pomona Senior Theses

In my thesis, I examine how various forms of news media from the 1900s to present day in Ohlone territory have framed a particular hegemonic ideology around fire that matches early colonial journal entries – an ideology that is fueled by fear, sensationalism, and exclusion and directly commits violence against the Ohlone people. I investigate this time period because this is when modern news corporations, like The New York Times, rose into prominence. Specifically, I question how media representations of fire inform regulations over controlled burns; what are the similarities in discourse between media framing and policy? What tactics are …

“Nice To See You”: Addressing Plant Awareness Disparity With Locally Specific Ecological And Cultural Plant Education, Cecilia Muecke

Pomona Senior Theses

Plants form the basis of life on Earth. However, due to the phenomenon of plant awareness disparity, a self-perpetuating cycle whereby plants are overlooked on an individual and global level ensues. Lack of plant awareness leads to a lessened emphasis on plants in sustainability and conservation as well as biology education, resulting in negative impacts on biodiversity and food security. The severity of plant awareness disparity is dependent on cultural context, and Indigenous and/or traditional societies are less likely to experience plant awareness disparity because of they are typically more connected to plants for their everyday needs. Native plants are …

Rwanda's Natural Remedies: A Fresh Look At The Fight Against Malaria, Leandre Nsabimana Ndisanze

Pomona Senior Theses

Malaria remains one of the most devastating parasitic diseases globally, disproportionately affecting sub-Saharan Africa, where Rwanda continues to face high transmission rates and rising resistance to conventional interventions. Despite decades of control efforts, the emergence of drug-resistant Plasmodium falciparum and insecticide-resistant Anopheles mosquitoes threatens to reverse recent gains. This research responds to the urgent need for sustainable and culturally relevant alternatives by exploring the therapeutic potential of Rwandan traditional medicinal plants. These indigenous remedies, long used by local healers, offer an underexplored reservoir of bioactive compounds that may provide the basis for novel antimalarial drugs.

The central hypothesis of this …

Florida Scrub Is Nuts (And Drupes): Fruit Production And Consumption In Florida’S Fire-Prone, Xeric Uplands, Warren G. Abrahamson Ii

Faculty Journal Articles

This is a story about how plant size, fire, and climate affect fruit and nut production (i.e., mast) in Florida’s fire-prone, xeric uplands and the importance of mast as food for wildlife. To set the stage, let’s first explore the primary mast-producing plants of Florida’s xeric uplands: oaks, hickory, and palmettos.

A Vascular Flora Of The Sacatar Trail Wilderness, Sierra Nevada, California, Kimberly Schaefer Pivetti

CGU Theses & Dissertations

The Sacatar Trail Wilderness in Inyo and Tulare counties, California, encompasses a bioregional transition zone in the southeastern Sierra Nevada. The ca. 233 km 2 (90 mi 2 ) study area, located 32 km (20 mi) northwest of the city of Ridgecrest, ranges from 930 to 2698 m (3051–8851 ft) in elevation. The highly diverse California Floristic Province, Great Basin, and Mojave Desert converge in the region including the Wilderness creating conditions to support a broad spectrum of vegetation assemblages. The Bureau of Land Management has prohibited vehicular activity in this area since its wilderness designation in 1994; consequently, recent …

A Decision-Space Model Explains Context-Specific Decision Making, Dirk W. Beck, Cory N. Heaton, Luis D. Davila, Lara I. Rakocevic, Sabrina M. Drammis, Danil Tyulmankov, Atanu Giri, Shreeya Umashankar Beck, Qingyang Zhang, Michael Pokojovy, Kenichiro Negishi, Alexis A. Salcido, Neftali F. Reyes, Andrea Y. Macias, Serina A. Batson, Paulina Vara, Raquel J. Ibáñez Alcalá, Safa B. Hossain, Graham L. Waller, Laura E. O'Dell, Travis M. Moschak, Ki A. Goosens, Alexander Friedman

Mathematics & Statistics Faculty Publications

Optimal decision-making requires consideration of internal and external contexts. Biased decision-making is a transdiagnostic symptom of neuropsychiatric disorders. We created a computational model demonstrating how the striosome compartment of the striatum constructs a context-dependent mathematical space for decision-making computations, and how the matrix compartment uses this space to define action value. The model explains multiple experimental results and unifies other theories like reward prediction error, roles of the direct versus indirect pathways, and roles of the striosome versus matrix, under one framework. We also found, through new analyses, that striosome and matrix neurons increase their synchrony during difficult tasks, caused …

Mobula, Bioinspiration, Filter Feeding, Form And Function, J. B. Teeple, S. R. Kahane-Rapport, K. E. Cohen, L. Hamann, J. A. Strother, E. W.M. Paig-Tran

Biological Sciences Faculty Publications

Mobulas (manta and devil rays) are large-scale ram filter feeders that separate planktonic food particles from large volumes of water with minimal clogging. This contrasts with most human-made filters that can suffer from problematic clogging requiring additional mechanisms for clearing blocked surfaces and maintaining performance. Prior studies have shown that mobulas employ a unique mechanism referred to as ricochet separation to filter feed, whereby captive vortices in filter pores cause particles to bounce off the filter surfaces and away from the filter pores. This mechanism enables the filtration of particles smaller than the pore size and reduced clogging. However, few …

Analog Digital Frame: User Centered Design In Arts-Based Neighborhood Resilience Modeling, David Robledo, Sydney F. Walker, Zlatka Rebolledo Sánchez, Richard Nickel

English Faculty Publications

We explicate a mapping of digital documents through computer machinery as an analog frame for Research and Design in neighborhood environmental literacy, within an applied interdisciplinary ecosystem theory. In the arena of communicating localized climate phenomena and solutions, the design of communication frameworks has provided a core methodology to map climate adaptive conclusions as analogs for actionable community interpretive media. In this experience report, we articulate User Centered Design (UCD) in project research and development, while reporting on difficulties and solutions in shaping an analog digital frame to integrate student driven research and design with mentor based interdisciplinary project theorization. …

Amorphous Quininium Aspirinate From Neat Mechanochemistry: Diffracting Nanocrystalline Domains And Quick Recrystallization Upon Exposure To Solvent Vapours, Silvina Pagola, James Howard, Johannes Merkelbach, Danny Stam

Chemistry & Biochemistry Faculty Publications

Quininium aspirinate is mechanochemically prepared as a crystalline solid by liquid-assisted grinding, or as an amorphous phase (as determined by X-ray powder diffraction), by neat grinding or neat ball milling. Our previous work demonstrated using FT-IR spectroscopy that a mechanochemical reaction had occurred in the mechanically treated neat mixtures. Herein is reported that microcrystal electron diffraction (microED) afforded the discovery of two diffracting micron-size particles in the amorphous powder synthesized by manual grinding, among a majority of non-diffracting particles. Remarkably, microED data of one of them led to the known lattice parameters of quininium aspirinate. Furthermore, this so-called 'X-ray amorphous' …

Protonic Capacitor Cell Energetics: Transmembrane-Electrostatically Localized Protons/Cations, James Weifu Lee

Chemistry & Biochemistry Faculty Publications

The transmembrane-electrostatically localized protons/cations charges (TELPs/TELCs) theory can serve as a theoretical framework to better explain cell electrophysiology and elucidate bioenergetic systems including both delocalized and localized protonic coupling. According to the TELCs model, the excess positive charges of TELCs at one side of the membrane are balanced by the excess negative charges of transmembrane-electrostatically localized hydroxides anions (TELAs) at the other side of the membrane. Through the TELCs-membrane-TELAs capacitor model, the energetics of oxidative phosphorylation have recently been better elucidated in mitochondria and alkalophilic bacteria, leading to the identification of a novel Type-B energetic process. Both the TELCs model …

Fire History, Ecological History, And Historical Human-Environment Interactions In The Northern Great Plains, James Victor Benes

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

This dissertation explores the spatiotemporal signatures of fire in North American grassland ecosystems. In three chapters, I explore different aspects of fire history in the northern Great Plains. Chapter 1 is an introduction. Chapter 2 focuses on the Euro-American Settlement Period (ca. 1850–1950 C.E.). Fire histories from six different lakes in the Nebraska Sandhills are compared with two periods of historic drought and the establishment of United States Post Offices. Five of the six records show increased levels of biomass burning during the initial phases of Euro-American Settlement. Chapter 3 is a long-term (ca. 15,000 years), high-resolution fire history and …

Strolling The Lingnan Garden, 2022-2024 = 涓流彩園錄, 第二卷 : 下冊, Lingnan Gardeners, Centre For Cultural Research And Development, Lingnan University

Lingnan Gardeners Publications 嶺南彩園刊物

No abstract provided.