Life Sciences Commons^™

Cisplatin Palbociclib Combination Differentially Modulates Pten Akt Signaling Via Hsp90 In Hepatocellular Carcinoma Cells., Eman Ramadan

Pharmacy

Hepatocellular carcinoma (HCC) presents a significant global health challenge, marked by high mortality and recurrence. This study investigated the synergistic potential of cisplatin and palbociclib (C + P) against HCC cell lines. RT-qPCR revealed that C + P significantly downregulated HCC-related genes, including Hsp90, β-catenin, and components of the PI3K/AKT/mTOR pathway, compared to cisplatin alone and controls. Western blotting confirmed a reduction in phosphorylated AKT (P-AKT) with palbociclib and C + P, while PTEN, a tumor suppressor, was upregulated in the C + P group. Annexin V-FITC assays demonstrated a substantial increase in apoptosis in palbociclib and C + P …

Judgment Bias, Fear, And Stress Responses Of Red Junglefowl And Athens Canadian Random Bred Chickens, Victor J. Oyeniran, Rosemary H. Whittle, Sara K. Orlowski, Shawna L. Weimer

Poultry Science Faculty Publications and Presentations

Understanding how poultry perceive and interpret their environment is essential to enhancing their welfare. Animal welfare science relies on measures of the behavioral and physiological components of affective states (positive and negative) as welfare indicators. There has been growing interest in using the judgment bias test (JBT) to study birds' affective states by assessing their responses to ambiguous cues. The objective of this study was to investigate the affective state of two chicken breeds with different evolutionary histories: the Red Junglefowl (RJF), the primary ancestor of modern chickens, and the Athens Canadian Random Bred (ACRB), a rustic domesticated breed, using …

A Descriptive Analysis Of The Current State Of Peace Officer Standards And Training Physical Fitness Requirements Across The Usa, Robert G. Lockie, Joseph Dulla

International Journal of Exercise Science

International Journal of Exercise Science 18(8): 543-560, 2025. Many law enforcement organizations are experiencing recruitment and retention challenges, of which physical fitness can be a factor. The study purpose was to collate information from Peace Officer Standards and Training (POST) Commission staff in each USA state to document current fitness mandates and policies for recruits/officers. A 10-question survey was allocated to an appropriate representative from every state POST organization. The survey collected information regarding minimum state-mandated fitness standards for academy entry and exit; minimum state-mandated requirements for fitness programming, including safety plans; education/training requirements for fitness training instructors; and minimum …

Smart Glove Pitch Type Discrepancy Detection Algorithm For Youth And Collegiate Baseball Pitchers, Ethan B. Hemmerlin

Master's Theses

Pitch tipping refers to unintentional variations in a pitcher’s motion that reveal the pitch type, often due to inconsistent arm speed when throwing off-speed pitches like changeups. This issue is common in youth players with underdeveloped mechanics and makes it easier for batters to predict pitches. Wearable sensors, such as Inertial Measurement Units (IMUs), provide a non-invasive way to quantify these subtle motion discrepancies. This study analyzed linear acceleration data from a glove arm IMU for 14 pitchers, evenly split between high school-aged (13-15 years) and college-aged (18-23 years) groups. The aim was to examine the linear accelerations of the …

Effects Of Spirulina And Nannochloropsis Supplementations On The Meat Quality And Sensory Properties Of Broiler Carcasses, Shreyash S. Patel

Master's Theses

Microalgae are a promising feed ingredient due to their high content of proteins, lipids, polysaccharides, vitamins, pigments, and enzymes. This study evaluated the effects of algae as a dietary supplementation on the appearance, quality, and sensory attributes of broiler carcasses. In each of experiment I and II, a total of 54 Ross-708 broilers were randomly obtained from the pool of 270 broilers in the 18 floor pens of soybean-mealbased diets at 0, 2.5%, and 5% Spirulina (Experiment I) and Nannochloropsis (Experiment II) supplements in the Poultry Unit at California Polytechnic State University (Cal Poly, San Luis Obispo, CA). Broiler carcasses …

Dataset Supporting The Manuscript Promoting Awareness And Adoption Of Biochar As A Soil Amendment: Effect Of Message Framing, Tong Wang, David Wiltse, Filip Viskupic, Akinsola Oyebanji

South Dakota Farm Survey

A farmer survey was conducted from February 22nd to March 9th, 2025 to test the impact of message framing on farmers’ learning and adoption decisions of biochar. The survey targeted operators of farms in Eastern South Dakota where the production of corn requires chemical fertilizer is intensively used as a crucial input. Biochar as a soil amendment has the potential to serve as a partial substitute for chemical fertilizer in our studied region. A sample of 10,000 South Dakota farming operations east of the Missouri River (where climate favors row crop production) was randomly drawn from those who participated in …

Art, Play, And Neuroplasticity: Advancing Dementia Care Through Art-Enriched Environments, Piper Hutson, James Hutson

Faculty Scholarship

Dementia represents a global public health crisis, characterized not only by progressive memory loss but by a pervasive decline in cognitive flexibility, social functioning, and overall quality of life. Traditional therapeutic modalities, often limited to pharmacological management and cognitive training, have yielded only modest gains in symptom mitigation and patient well-being. Recent advances in neuroscience have illuminated the brain‘s capacity for neuroplasticity—its inherent ability to adapt, reorganize, and form new neural connections even in advanced age and the presence of neurodegenerative conditions. This emerging understanding invites a critical re-evaluation of dementia care strategies, highlighting the potential of non-pharmacological interventions to …

Covid-19 Pandemic And Recovery Experiences: A Case Study With Montana Family And Consumer Science Cooperative Extension Agents, Hayley M. Somerfeld, J. Mitchell Vaterlaus, Nicole C. Wanago, Brianna Routh, Alison Brennan

Journal of Human Sciences and Extension

The present study examined the impact of COVID-19 and recovery experiences among Family Consumer Science (FCS) Extension Agents in Montana. All participants (n = 10) completed a survey with fixed-item and open-ended responses, and six also participated in in-depth interviews. A deductive qualitative case study approach was used. Agent experiences were analyzed using The Sendai Framework for Disaster Risk Reduction (SFDRR) four action priorities: (1) understanding risk, (2) strengthening disaster risk governance, (3) investing in disaster risk reduction for resilience, and (4) enhancing disaster preparedness for effective response and to “build back better” in recovery, rehabilitation, and reconstruction. Pandemic risks …

Enhancing Stability And Emissions In Metal Halide Perovskite Nanocrystals Through Mn2+ Doping, Thi Thu Trinh Phan, Thi Thuy Kieu Nguyen, Trung Kien Mac, Minh Tuan Trinh

Chemistry and Biochemistry Student Research

Metal halide perovskite (MHP) nanocrystals (NCs) offer great potential for high-efficiency optoelectronic devices; however, they suffer from structural softness and chemical instability. Doping MHP NCs can overcome this issue. In this work, we synthesize Mn-doped methylammonium lead bromide (MAPbBr₃) NCs using the ligand-assisted reprecipitation method and investigate their structural and optical stability. X-ray diffraction confirms Mn²⁺ substitution at Pb²⁺ sites and lattice contraction. Photoluminescence (PL) measurements show a blue shift, significant PL quantum yield enhancement, reaching 72% at 17% Mn²⁺ doping, and a 34% increase compared to undoped samples, attributed to effective defect passivation and …

Empowering Community Through Extension: Identifying Community Health And Wellness Strengths And Needs, Allyson A. Spears, Alexis Zickafoose, Emily Wintermute, Peng Lu, Matthew Baker, Scott Cummings

Journal of Human Sciences and Extension

Health and wellness are foundational to thriving communities, yet many Americans face barriers that impact their access to essential health education and services. This study used a community-based participatory research (CBPR) approach to assess the perceived strengths and needs of health and wellness resources of rural and urban Texas communities. Data were collected through a statewide survey (n = 9,418), targeting key aspects of health and wellness. The results identified critical strengths, including school lunch programs and grocery stores accepting electronic benefit transfers (EBT), while reducing obesity and addressing mental health emerged as top community needs in both urban and …

Cross-Institutional Dental Electronic Health Record Entity Extraction Via Generative Artificial Intelligence And Synthetic Notes, Yao-Shun Chuang, Chun-Teh Lee, Guo-Hao Lin, Ryan Brandon, Xiaoqian Jiang, Muhammad F Walji, Oluwabunmi Tokede

Faculty, Staff and Student Publications

Background: While most health-care providers now use electronic health records (EHRs) to document clinical care, many still treat them as digital versions of paper records. As a result, documentation often remains unstructured, with free-text entries in progress notes. This limits the potential for secondary use and analysis, as machine-learning and data analysis algorithms are more effective with structured data.

Objective: This study aims to use advanced artificial intelligence (AI) and natural language processing (NLP) techniques to improve diagnostic information extraction from clinical notes in a periodontal use case. By automating this process, the study seeks to reduce missing data in …

Baseline Predictors Of Responders To Auricular Point Acupressure In Chronic Low Back Pain, Nada Lukkahatai, Wanqi Chen, Jennifer Kawi, Hulin Wu, Claudia M Campbell, Johannes Thrul, Xinran Huang, Paul Christo, Constance M Johnson

Faculty, Staff and Student Publications

Background: Chronic low back pain (cLBP) is a major cause of disability, with varied patient responses to treatments. Auricular point acupressure (APA) has shown potential as a non-pharmacological intervention, but individual responses may differ significantly.

Objective: This study aimed to determine the predictability of baseline characteristics, including functional disability, symptom severity, and treatment expectancy, on clinically significant responses to APA in reducing pain and improving function.

Methods: A secondary analysis was performed using data from a randomized controlled trial with 263 cLBP patients. Participants were randomly assigned to targeted APA (T-APA), non-targeted APA (NT-APA), or to a control group. APA …

An Unusual Trumpeter Swan (Cygnus Buccinator) In Nebraska: Albinism Or Leucism?, Jacob C. Cooper, Peggy M. Huss, Eugene A. Huryn, Cody Mcgregor

Nebraska Bird Review

On 26 January 2025, we observed a large mixed flock of Trumpeter Swans (Cygnus buccinator) and Tundra Swans (C. columbianus) at Hansen Reservoir ca. 7 miles (11 km) NNW of North Platte along the east side of Nebraska Highway 97. The flock of 158 swans consisted of 4 “Whistling” Tundra Swans (C. c. columbianus), 153 typical Trumpeter Swans, and one individual that we were not immediately able to identify. Our unknown swan was in the company of a family group of Trumpeter Swans but immediately stood out due to its broad yellow base of …

Nebraska Ornithologists' Union Tri-State Meeting With Iowa Ornithologists' Union And South Dakota Ornithologists' Union, South Sioux City, Nebraska, May 16-18, 2025

Nebraska Bird Review

The NOU Spring Meeting was held at the Salvation Army Western Plains Camp in South Sioux City, Nebraska, in conjunction with the IOU and SDOU, with approximately 180 attending. The organizers of the meeting included Chris Anderson and Mark Schmidtbauer from South Dakota, Tyler Harms and Kevin Murphy from Iowa, and Ruth Stearns, Joel Jorgenson, and Paul Pearson from Nebraska.

A scientific poster session featuring the work of 9 ornithological researchers from UN-Kearney, USD-Vermillion, and Hitchcock Nature Center was held Friday evening. Thanks to Dr. Jacob Cooper from UNK, Dr. David Swanson from USD, and the students who participated.

Field …

The Role Of Socioeconomic Status In Shaping Associations Between Sensory Association Cortex And Prefrontal Structure And Implications For Executive Function, Maya L. Rosen, Divyangana Rakesh, Rachel R. Romeo

Neuroscience: Faculty Publications

Socioeconomic status (SES) is associated with widespread differences in structure of temporal, parietal, occipital, and frontal cortices. Development of sensory processing regions—in particular visual association cortex (VAC) and auditory association cortex (AAC)—may scaffold development of the prefrontal cortex (PFC). Experiences that correlate with SES like cognitive stimulation and language may influence VAC and AAC development, in turn allowing the PFC to resolve conflicts between similar stimuli. SES-related differences in these regions may partly explain differences in executive function (EF) skills. Here, we use structural equation modeling of longitudinal data from the Adolescent Brain and Cognitive Development study to test the …

Discovery Of Alcohol Dehydrogenase (Adh) As A Potential Vaccine Target From Mycolicibacterium Smegmatis Extracellular Vesicles Via Immunoproteomics, M. Jayaram, N. Othman, Khayriyyah Mohd Hanafiah, M. Azlan, N. Arifin

Biology Faculty/Staff Publications

Mycobacterium tuberculosis (MTB), the causative agent of tuberculosis, releases extracellular vesicles (EVs) that impair macrophage functions and circulate bacterial components to modulate the host immune response. While EVs are increasingly investigated as new vaccines and biomarkers, studying MTB EVs is challenging due to the slow growth rate and pathogenic properties of MTB. Mycolicibacterium smegmatis (MSMEG), a non-pathogenic surrogate with a faster growth rate, offers a safer and more convenient option for laboratory studies due to its similarities to MTB. In this study, we explore the antigenic properties of MSMEG EVs to assess their potential use in developing safer tuberculosis vaccine …

Nou Tri-State Meeting—South Sioux City [Spring 2025 Nou Meeting Species Tally]

Nebraska Bird Review

Bird species tally (185) for the Spring 2025 NOU Tri-State Meeting, South Sioux City (Nebraska Ornithologists’ Union Tri-State Meeting with Iowa Ornithologists’ Union and South Dakota Ornithologists’ Union, South Sioux City, Nebraska, May 16–18, 2025)

Understanding The Habitat Associations Of Nearshore Rockfishes Inside And Outside Of Marine Protected Areas Using Hook-And-Line Data, Kaila Raeanne Fritch

Master's Theses

Habitat is an important driver in shaping the distribution of a range of species. For demersal fish species, benthic habitat characteristics can drive distribution and community structure. Understanding the relationship between habitat and species distribution in important, especially for exploited species such as rockfishes (Sebastes spp.). Increased data on the relationship between rockfish and benthic habitat characteristics could enhance marine protected areas (MPAs) and management practices which are used to protect rockfishes along the U.S. West Coast. To address this, we used hook-and-line, catch-and-release data that were collected inside two MPAs on the central coast of California, and …

The Effect Of Whole Fruits High In Anthocyanins On Markers Of Glycemic Control In Women, Sophia C. Johnson

Master's Theses

Cardiometabolic diseases are a significant health burden worldwide and remain the leading cause of death among adults in the United States. Type 2 diabetes mellitus (T2DM) is a central component of cardiometabolic dysfunction and substantially increases the risk for cardiovascular disease. As women enter the post-menopausal period, significant declines in estrogen levels lead to a heightened risk of T2DM in this population. Observational and experimental evidence has shown potential for fruits high in anthocyanins (ACs) to reduce the risk of T2DM and improve glycemic control. This systematic review and meta-analysis examined the current research on whole fruits high in ACs …

Subalpine And Alpine Plant Community Turnover In Yosemite National Park Following 30 Years Of Climate Warming, Rachel Friesen

Master's Theses

Subalpine and alpine regions in California are warming more rapidly than lower elevations. In response, high-elevation plants may acclimate or adapt to the new climate, move to higher elevations or latitudes, persist in refugia, or go extinct. While models predict substantial declines in subalpine and alpine plant communities due to discontinuous population distributions and lack of upslope area, actual responses may vary due to lags in dispersal, establishment, and extinction. Although shifts in high-elevation plant communities have been documented in other regions of the world, such changes have not yet been studied in Yosemite National Park. To assess high-elevation plant …

The Mayfly Newsletter, Donna Giberson

The Mayfly Newsletter

The Mayfly Newsletter is the official newsletter of the Permanent Committee of the International Conferences on Ephemeroptera.

Abstracts Of Posters Presented At South Sioux City Nou Meeting

Nebraska Bird Review

A scientific poster session featuring the work of 9 ornithological researchers from UN-Kearney, USD-Vermillion and Hitchcock Nature Center was held Friday evening. Thanks to Dr. Jacob Cooper from UNK, Dr. David Swanson from USD, and the students who participated.

Wandering from the River: On- and Off-River Abundance of Interior Least Terns along the 59-Mile Reach of the Missouri National Recreational River. Zoe Donelan, Hannah Slattery, Kyle Jorgensen, and David L. Swanson, University of South Dakota, Department of Biology.

Preliminary Results of Raptor Migration Monitoring at Hitchcock Nature Center. Kellie Hayden, Jerry Toll, and Sandy Reinken, Hitchcock Nature Center, Pottawattamie Conservation, …

Spring Field Report, March–May 2025, W. Ross Silcock

Nebraska Bird Review

Perhaps the most important takeaway this spring is the continuing phenomenon of large numbers of species with early spring arrival dates. Although about half as many as 2024, there were 64 examples of either tied or new early arrival dates, most obviously of insectivores such as swifts, flycatchers, vireos, swallows, pipits, longspurs, and 10 warbler species. Among these, record or near-record early were Whip-poor-will, Black-necked Stilt, Marbled Godwit, Black Tern, Olive-sided Flycatcher, Purple Martin, Rock Wren, Gray Catbird in central, Spotted Towhee in north and west, and Hooded Warbler.

Following along logically perhaps with early arrival dates were a number …

The Nebraska Bird Review (June 2025) 93(2), Whole Issue

Nebraska Bird Review

Spring Field Report, March–May 2025, by W. Ross Silcock

NOU Spring Field Days and Annual Meeting in South Sioux City, May 16–18, 2025

Abstracts of posters presented at Spring 2025 NOU Meeting

Spring 2025 NOU Meeting Species Tally

An Unusual Trumpeter Swan (Cygnus buccinator) in Nebraska: Albinism or Leucism? by Jacob C. Cooper, Peggy M. Huss, Eugene A. Huryn, and Cody McGregor

Subscription and Organization Information

When Trees Disappear: Understory Successional Implications Of Sudden Oak Death And High-Severity Wildfire In Big Sur, California, Sam J. Balthazard

Master's Theses

The rapidly emerging disease sudden oak death, caused by the non-native pathogen Phytophthora ramorum, is transforming coastal California forests by driving extensive mortality of tanoak (Notholithocarpus densiflorus) and increasing fire severity. As a novel disturbance, sudden oak death and its interaction with wildfire may catalyze unexpected shifts in successional trajectories and reconfigure forest understory communities. Furthermore, how tanoak influences the forest understory and how its decline might reshape understory dynamics remain poorly understood. We hypothesize that disease alone and the compounding disturbances of disease and wildfire alter successional dynamics, and that the potential functional extinction of N. …

Genetic Analysis Of Psychosis Biotypes: Shared Ancestry-Adjusted Polygenic Risk And Unique Genomic Associations, Cuihua Xia, Ney Alliey Rodriguez, Carol A. Tamminga, Matcheri S. Keshavan, Godfrey Pearlson

School of Medicine Publications

The Bipolar-Schizophrenia Network for Intermediate Phenotypes (B-SNIP) created psychosis Biotypes based on neurobiological measurements in a multi-ancestry sample. These Biotypes cut across DSM diagnoses of schizophrenia, schizoaffective disorder, and bipolar disorder with psychosis. Two recently developed post hoc ancestry adjustment methods of Polygenic Risk Scores (PRSs) generate Ancestry-Adjusted PRSs (AAPRSs), which allow for PRS analysis of multi-ancestry samples. Applied to schizophrenia PRS, we found the Khera AAPRS method to show superior portability and comparable prediction accuracy as compared with the Ge method. The three Biotypes of psychosis disorders had similar AAPRSs across ancestries. In genomic analysis of Biotypes, 12 genes, …

Oviposition-Deterrent And Repellent Effects Of Synthetic Plant-Derived Compounds On The Pulse Beetle, Callosobruchus Chinensis (Coleoptera: Chrysomelidae: Bruchinae), Akki Suma, M Rajadurai, Ac Lokesh, Mb Anusha, S Krishna Kumar

The Philippine Agricultural Scientist

The pulse beetle, Callosobruchus chinensis (Linnaeus, 1758), is a major stored pest of pulses (Fabaceae) in India. While effective control of this widespread and destructive insect pest is essential, continuous insecticide use poses environmental concerns, thereby necessitating alternative, eco-friendly control methods. This study evaluated the oviposition-deterrent effects of 7 synthetic compounds (linalool, guaiene, citral, estragole, 4-nonanone, alloaromadendrene, and citronellal) on C. chinensis. Linalool showed the strongest suppression of egg-laying at higher concentrations, with the lowest egg count at 500 ppm compared to the control, followed by citral, citronellal, and guaiene. Linalool was also effective in suppressing egg hatching at …

Effects Of Probiotic Supplementation On Growth Performance, Nutrient Digestibility, And Enteric Methane Emission Of Growing Dairy Buffaloes (Bubalus Bubalis L.), Christian Lualhati, Karl Reginald Managa, Charity Castillo, Phoebe Lyndia Llantada, Arnel Del Barrio

The Philippine Agricultural Scientist

This study assessed the impact of probiotics on the growth performance, nutrient digestibility, and enteric methane emission of 4 growing dairy buffaloes (Bubalus bubalis L.) with similar body weight, age, and sex. The animals were allocated into 4 treatments following a Latin Square design: control/no probiotic supplement (Trt 1); with 5 mL of probiotic supplement (Trt 2); with 10 mL of probiotic supplement (Trt 3); and with 15 mL of probiotic supplement (Trt 4). The growers were offered the dietary treatments at the start of the adaptation phase up to the end of the experimental phase per period. Animals …

Antimicrobial Activity In Auxin-Induced Formation Of In Vitro Culture- Derived Aloe Vera Roots, Eufrocinio Marfori, Bernadette Rapiz

The Philippine Agricultural Scientist

Aloe vera is a perennial, succulent plant which has been used in folk medicine for wound-healing, as its roots have been reported to exhibit remarkable antimicrobial properties. However, collecting the roots is detrimental to plant growth and development. Hence, an alternative system of producing A. vera roots must be developed. Tissue culture is a potential tool to obtain A. vera root biomass, which can serve as raw materials for the large-scale production of antimicrobial compounds. In this study, individual shoots taken from the multiple shoot culture of A. vera were induced to form roots in MS medium supplemented with 1 …

Isolation And Enumeration Of Salmonella Enterica From Fresh Lettuce (Lactuca Sativa L.) Leaves And Its Susceptibility To Different Washing Protocols, Meinard Talplacido, Angella Melissa Carlos, Bryan Rey Oliveros, Ida Dalmacio

The Philippine Agricultural Scientist

Lettuce is one of the most consumed fresh produce items in the country, but it also serves as a vehicle for human pathogens, posing a risk of foodborne illness. This study used conventional microbiological and molecular analyses to obtain the concentration and prevalence of Salmonella enterica in fresh Green Ice and Iceberg lettuce leaves from selected supermarkets in Los Baños, Laguna, Philippines. The efficacy of different household washing procedures in reducing S. enterica on lettuce leaves was also investigated. Of the 19 composite Green Ice lettuce and 21 composite Iceberg lettuce samples tested, 5.26% and 4.76%, respectively, were contaminated with …