Life Sciences Commons^™

Co-Designing A Digital Nutrition Intervention For Individuals With Severe Mental Illness Using The Behaviour Change Wheel, Ciara O'Sullivan, Tara Coppinger, Indika Dhanapala, Alison Merrotsy

Publications

Title: Co-Designing a Digital Nutrition Intervention for Individuals with Severe Mental Illness Using the Behaviour Change Wheel

Purpose: This study aims to develop a digital nutrition intervention for individuals with severe mental illness (SMI) residing in high-support mental health hostels, using a theoretically informed co-design approach.

Design/methodology/approach: This study will employ a participatory, mixed-methods approach, incorporating Public and Patient Involvement (PPI) to ensure user-centred intervention design. The Behaviour Change Wheel (BCW) guides the selection of intervention functions, Behaviour Change Techniques (BCTs) and delivery methods. The study will consist of three phases: (1) Preparation, (2) Co-Design, and (3) Finalisation.

Findings: Data …

Creation Of An Open Access Textbook, Sue Ann Gardner

University of Nebraska-Lincoln Libraries: Presentations

Slide presented during the PRCA Research Celebration, June 3, 2025, in the University Libraries, University of Nebraska-Lincoln titled Creation of an Open Access Textbook, about the development and publication of Concepts in Animal Parasitology, co-edited by Scott L. Gardner and Sue Ann Gardner and published by Zea Books (Lincoln, Nebraska, USA) in 2024.

Pyruvate Dehydrogenase Kinase 4 Mediates Sex Specific Outcomes In Sepsis Induced Cardiomyopathy, John Yap

Dissertations

During sepsis, males often experience worse cardiac outcomes than females. Here, we identified pyruvate dehydrogenase kinase 4 (PDK4) as a key contributor to this disparity. PDK4 is a mitochondrial protein that regulates glucose utilization by inhibiting pyruvate dehydrogenase (PDH). Using a mouse model of endotoxemia, we induced acute inflammation with a sublethal dose of lipopolysaccharide (LPS), which caused cardiac dysfunction and significantly increased myocardial PDK4 expression in males but not females. Cardiac-specific PDK4 overexpression promoted LPS-induced cardiac dysfunction in both sexes, whereas cardiac-specific PDK4 knockout provided protection. In wild-type males, LPS disrupted cardiac metabolism by decreasing PDH activity and fatty …

Interrogating The Dispersal Process And Relevant Genes In Vibrio Fischeri, Jeremy Esin

Dissertations

Biofilms are aggregates of bacteria encased in a matrix that shields bacteria from environmental hazards. These structures are formed in response to cues from the environment and drive colonization of new niches. Biofilms can be both beneficial and detrimental. Largely, research focuses on biofilms that have negative outcomes in clinical, environmental, and economical settings. However, the value of beneficial biofilms is recognized and studied. The marine bacterium Vibrio fischeri is part of a model system for studying symbiotic biofilms in a eukaryotic host. V. fischeri produces a transient biofilm at the surface of a specialized light organ and then disperses …

Culture Harder: Use More Specimen To Increase Methicillin-Resistant Staphylococcus Aureus Culture Yield Relative To Pcr, Arvette E. Mitchell, Arpit P. Patel, Jennifer Dicandilo, Zachary W. Rebollido, Matthew Pettengill

Department of Pathology, Anatomy, and Cell Biology Faculty Papers

Methicillin-resistant Staphylococcus aureus (MRSA) causes considerable morbidity and mortality in both community-acquired and healthcare-associated infections, but detecting colonization with MRSA has been shown to improve patient outcomes in certain clinical settings. MRSA colonization detection has been carried out in a variety of ways, with molecular assays having superior sensitivity in most studies relative to culture, but culture is disadvantaged in some comparisons by utilization of low specimen volumes. We compared a commercial molecular assay to both low-volume (10 µl) and high-volume (650 µl) cultures and found that increasing the volume utilized for culture led to the detection of 25% more …

Suggested Vegetable Planting Dates For Utah, Shawn Olsen

All Current Publications

This fact sheet provides suggested planting dates for vegetables in various areas throughout Utah. It also identifies resources for starting seeds at home.

Plasma Insulin-Like Growth Factor-Binding Protein-7 Is Positively Associated With Age, Obesity, Mortality, And Cancer In Postmenopausal Women, Melissa C Orenduff, Carl F Pieper, Emma H Allott, Michael F Coleman, Su Yon Jung, Mara Z Vitolins, Jenifer I Fenton, Chu Chen, Candyce H Kroenke, Fred K Tabung, Ana Barac, Electra D Paskett, Michael N Pollak, Jennifer Hays-Grudo, Shine Chang, Stephen D Hursting

Faculty, Staff and Student Publications

Background: Predictors of premature death and cancer development are needed to more precisely identify individuals who may warrant preventive intervention. Circulating insulin-like growth factor (IGF)-binding protein-7 (IGFBP7) and, to a lesser extent, the IGFBP7/IGF-1 ratio are emerging biomarkers of renal and cardiovascular morbidity. However, their relationships with aging, obesity, mortality, and cancer risk remain unclear.

Methods: This hypothesis-generating study investigated plasma IGFBP7, IGF-1, and their ratio as predictors of all-cause mortality and the incidence of any cancer (excluding nonmelanoma skin cancer), obesity-related cancer (composite of 13 cancer types), and breast cancer in a large longitudinal cohort of postmenopausal women. We …

Restoration Of The Tumor Suppressor Function Of Y220c-Mutant P53 By Rezatapopt, A Small-Molecule Reactivator, Anna M Puzio-Kuter, Lizhong Xu, Mary Kate Mcbrayer, Romyr Dominique, Hongju H Li, Bruce J Fahr, Alyssa M Brown, Amy E Wiebesiek, Brandon M Russo, Chris L Mulligan, Hong Yang, Josh Battaglia, Kimberly A Robell, Dafydd H Thomas, Kuo-Sen Huang, Alexander Solovyov, Benjamin D Greenbaum, Jonathan D Oliner, Thomas W Davis, Melissa L Dumble, Melissa L Johnson, Shunbin Xiong, Peirong Yang, Guillermina Lozano, Marc M Fellous, Binh T Vu, Alison M Schram, Arnold J Levine, Masha V Poyurovsky

Faculty, Staff and Student Publications

Restoration of the tumor suppressor function of tumor-associated p53 mutants, including the Y220C substitution, has posed a significant challenge for therapeutic discovery. In this study, we describe rezatapopt (PC14586), part of a series of compounds designed to reactivate the p53 Y220C mutant. These compounds restore p53 tumor suppressor function by correcting its conformation and enabling it to bind DNA and activate downstream target genes, thus inducing antiproliferative changes in tumor cells. Our findings are supported by biochemical and structural analysis, in vitro and in vivo transcriptomics, and functional data, revealing the recovery of multiple aspects of the wild-type p53 program. …

Posterior Predictive Design For Phase I Clinical Trials, Chenqi Fu, Shouhao Zhou, J Jack Lee

Faculty, Staff and Student Publications

Interval-based designs represent cutting-edge adaptive methodologies for phase I clinical trials to identify the maximum tolerated dose (MTD). These designs exhibit robust performance comparable to more intricate, model-based designs, and their pretabulated decision rule enables them to be implemented as simply as the conventional algorithm-based designs. In this paper, we introduce the posterior predictive (PoP) design, a novel interval-based design that leverages advanced Bayesian predictive hypothesis testing techniques for dose escalation and de-escalation. Our work moves beyond the existing model-assisted interval-based designs by achieving global optimality in dose transition. Theoretically, the global optimality ensures that the proposed design can consistently …

Mutant P53 Gain Of Function: Why Many See It, Why Some Do Not, Guillermina Lozano, Carol Prives, Kanaga Sabapathy

Faculty, Staff and Student Publications

Mutations in the TP53 tumor-suppressor gene in human cancer are unique in that 60% to 70% are of the missense variety, resulting in a full-length protein that is often highly expressed in patients' tumors. These missense mutant proteins often exhibit pro-oncogenic activities (referred to as gain of function) in mouse models and human cell lines and correlate with poor cancer prognosis in some cases.

Prescribed Fire Leads A Pine Flatwoods Specialist Tree Frog To Seek Local Refugia, Ian Biazzo, Pedro F. Quintana-Ascencio

Faculty Publications

Pine savannas of the southeastern United States evolved with frequent low-intensity wildfires. Today, land managers use prescribed fires to restore this ecological process while also controlling vegetative fuel accumulation. Given the ubiquitous application of this management tool, studies should consider impacts on target and nontarget taxa in these systems. During fires, animal populations persist in the landscape when individuals emigrate to unburned areas or seek refugia within the burn zone; otherwise, they experience local extirpation. We examined the effect of prescribed fires on a pine flatwoods specialist frog, the pinewoods treefrog Dryophytes femoralis, in a frequently burned pine landscape. …

Present And Future Of Microbiome-Targeting Therapeutics, Lauren E Lynch, Rachel Lahowetz, Christian Maresso, Austen Terwilliger, Jason Pizzini, Valeria Melendez Hebib, Robert A Britton, Anthony W Maresso, Geoffrey A Preidis

Children’s Nutrition Research Center Staff Publications

A large body of evidence suggests that single- and multiple-strain probiotics and synbiotics could have roles in the management of specific gastrointestinal disorders. However, ongoing concerns regarding the quality and heterogeneity of the clinical data, safety in vulnerable populations, and the lack of regulation of products containing live microbes are barriers to widespread clinical use. Safety and regulatory issues must be addressed and new technologies considered. One alternative future strategy is the use of synthetic bacterial communities, defined as manually assembled consortia of two or more bacteria originally derived from the human gastrointestinal tract. Synthetic bacterial communities can model functional, …

Arts-Based Sustainability: From New York To Malawi, Martha B. Lerski

Publications and Research

Recognizing that libraries serve multiple constituencies and subject areas, this chapter documents and advocates for development of transdisciplinary arts-based research (ABR) and culture-related projects linked to environmental challenges. Libraries contribute collections and spaces, as well as the research of library and information scientists. Libraries are currently among invisible contributors to sustainability planning and services. The chapter will link this invisibility to the value of what visual arts refer to as negative space elements in subjects ranging from traditional ecological knowledge to environmental science. Library collections, projects, and research contribute to education for sustainable development (ESD) as required to achieve the …

Walk A Mile: Exploring Cause-Based Charity Event Participation As A Catalyst For Increasing Physical Activity Behavior, Kimberly S. Fasczewski, Andrey A. Sanko Posada, Meg Hopkins

Journal of Social, Behavioral, and Health Sciences

Despite the known benefits of physical activity (PA), more than half of U.S. adults do not meet the American College of Sports Medicine’s PA recommendations for health, often resulting in negative health outcomes. Developing programming that increases motivation for PA participation is critical for healthcare professionals to combat physical inactivity. Previous research has demonstrated that framing disease-based PA charity fundraising events as “helping a cause” improved motivation for PA due to the individual’s emotional connection to the cause. There is currently no congruent research on motivation for participation in cause-based charity events. Behavioral economics theory posits that cognitive, emotional, and …

Soybean Embryogenic Cell Culture Genetically Engineered, Gabriel Carrasquillo

Tennessee State University Alumni Theses and Dissertations

The inflation of population in the world has led to a high and consistent food demand which has reinvigorated worldwide interest to create high-yielding varieties of legumes. Soybean Glycine max (L.) Merrill, a member of this legume family has become one of the most essential food crops in the United States as well as in the world due its edible oil and protein content up to 20% and 54%, respectively. Because of its high importance as a food crop, major objectives in soybean breeding, improvement of protein and oil production need to be achieved. These objectives can be addressed using …

Allelopathy Of Western Red Cedar, Douglas Fir, And Western Hemlock On Seed Germination, Lainey E. Mendoza Ms.

Honors Projects

Understanding the allelopathic interactions of native Pacific Northwest conifers, Douglas Fir, Western Hemlock, and Western Red Cedar is crucial to understanding the interactions of each species within their ecological niche. As of now there is little research on allelopathy of PNW conifers, specifically how different damaged states of the conifer tissue could impact the germination rates and radicle growth of a model organism. Analyzing these allelopathic interactions may be crucial to help predict forest regeneration of the PNW in the future. Presented here is the analysis of Douglas Fir, Western Hemlock, and Western Red Cedar tissue that is undamaged, damaged, …

The Ecophysiology Of The Enigmatic Namib Succulent, Lithops Ruschiorum, Garrett Macleod Althausen

Environmental Studies Senior Theses

Water availability is the primary constraint for plant survival and reproduction in hyper-arid ecosystems. In such environments, plant fitness depends on an alignment between physiological traits and limited water resources. The Namib Desert of Namibia exemplifies this extreme selection pressure. Within the Namib Desert, Lithops ruschiorum, a succulent endemic to the gravel plains, exhibits habitat specificity and numerous physiological adaptations to cope with the hyper-aridity.

This research explores the intersection of two fitness strategies for L. ruschiorum: habitat selection and exploitation of alternative moisture sources. The first chapter explores the population dynamics and micro-site habitat conditions preferred by …

Application Of High-Pressure Processing And Real-Time Pcr For Preventing Foodborne Bacterial Diseases, Ranju Kafle

Tennessee State University Alumni Theses and Dissertations

Foodborne pathogens pose significant risks to public health and food safety, necessitating innovative control strategies. High-pressure processing (HPP) was evaluated as an intervention against Listeria monocytogenes in cold-smoked rainbow trout, with treatments at 500 MPa and 4.4 °C and 60.0 °C achieving up to a 5.03 log CFU/g reduction. The efficacy of nisin in suppressing Listeria monocytogenes and Listeria innocua during refrigerated storage was also examined, demonstrating significant microbial inhibition, particularly in the early storage phase, supporting its application as a hurdle technology. Additionally, the synergistic effects of HPP (400 MPa, 4 °C) and natural bioactive compounds, including lactic acid, …

Exploring Soft Polymeric Nanoparticles For Targeted Biomolecule Delivery In Plant Cells, Mohammad Rashid

Theses and Dissertations

Safe and efficient agricultural practices require innovative technologies. Nanotechnology offers promising solutions for advancing plant agriculture by enabling the targeted delivery of biomolecules such as agrochemicals and nucleic acids. This study focuses on developing a safe nanotechnological approach for delivering molecules of interest into plant cells. We investigated the ability of four types of polymeric nanoparticles such as Polyethylene glycol N, Nʹ-dibutylethylenediamine (PEG-DB), Polyethylene glycol 2-pyrrolidin-1-yl-ethyl-amine (PEG-PY), Polyethyleneimine (PEI), Arabinoxylan (AX)+, and Zein+ to form conjugates with plasmid DNA (pDNA). Our findings confirm that plant-derived cationic nanopolymers, AX+ and Zein+, effectively bind negatively charged pDNA (pGFPGUSPlus) carrying a GFP reporter …

A Comprehensive, Multi-Center, Immunogenomic Analysis Of Melanoma Brain Metastases, Lucy Boyce Kennedy, Amanda E D Van Swearingen, Marissa R Lee, Layne W Rogers, Alexander B Sibley, Jeff Sheng, Dadong Zhang, Xiaodi Qin, Eric S Lipp, Swaminathan Kumar, Aron Joon, Pixu Shi, Michael A Davies, Kouros Owzar, Carey K Anders, April K S Salama

Faculty, Staff and Student Publications

Background: Melanoma brain metastases (MBM) have a unique molecular profile compared to extracranial metastases (ECM). Description of the biological features and clinical outcomes of MBM will facilitate the design of rational therapies.

Methods: We examined the mutational landscape and gene expression profiles of MBM (74 patients) and ECM (34 patients) in paired patient samples from a previously published dataset with whole-exome sequencing (WES) and RNA sequencing (RNAseq) data from MD Anderson Cancer Center (MDACC). We also present findings from MBM from a new cohort of 14 patients from Duke University to strengthen investigation of somatic mutations and gene expression profiles. …

Mutation Of Smarca4 Induces Cancer Cell-Intrinsic Defects In The Enhancer Landscape And Resistance To Immunotherapy, Yawen Wang, Ismail M Meraz, Md Qudratullah, Sasikumar Kotagiri, Yanyan Han, Yuanxin Xi, Jing Wang, Kadir C Akdemir, Jack A Roth, Yonathan Lissanu

Faculty, Staff and Student Publications

Cancer genomic studies have identified frequent alterations in genes encoding components of the SWI/SNF chromatin remodeling complex, including SMARCA4 and ARID1A. Importantly, clinical reports indicate that SMARCA4-mutant lung cancers respond poorly to immunotherapy and have dismal prognosis. In this study, we corroborated the clinical findings by using immune-humanized, syngeneic, and genetically engineered mouse models of lung cancer harboring SMARCA4 deficiency. Specifically, models with SMARCA4 loss showed decreased response to anti-PD-1 immunotherapy associated with significantly reduced infiltration of dendritic cells and CD4+ T cells into the tumor microenvironment. SMARCA4 loss in tumor cells led to profound downregulation of STING1, IL1β, and …

Sire Evaluations: Boer, Kiko, And Spanish Kid Weaning Weights In The Southeast, Alicia D. Aldridge

Tennessee State University Alumni Theses and Dissertations

Sire Evaluations: Boer, Kiko, And Spanish Kid Weaning Weights in the Southeast Research was completed with weaning weight ratio (WWR) and expected progeny difference (EPD) efficiency values compared from pedigree data across six seasons (2003-2009). Four models: WWR, 90- day unadjusted, adjusted, and new adjusted WW models compared sire breed, dam breed, month of birth, dam age, sex, litter size at birth, litter size at 90 day weaning age, and birth year effects. Correction factors were re-adjusted when non-genetic effects (sex, dam age, and litter size at birth and weaning) remained significant (p<0.05) in the original 90-day adjusted WW models. The new correction factors eliminated non-genetic effects (p>0.05). Sire breed effects were not significant …

Isolating Novel Enzymes And Microorganisms For Use In Food Waste Fermentation, Shannon Marie Jeffries

Tennessee State University Alumni Theses and Dissertations

In the United States, upwards of 2 billion pounds of food waste are discarded yearly, an amount equivalent to $161 billion or 30-40% of all food production, making up the largest portion of landfill waste (Buzby, 2022). A small amount of food scraps are recycled through compost and re-purposed into animal feed, but these processes are costly, have minimal benefits, and have limitations (EPA, 2024). One of the most promising routes is converting food waste into value added products through microbial fermentation. Food waste is rich in carbohydrates, proteins, lipids, vitamins, and other trace minerals, making it the ideal environment …

Seed Careers App Impact On Nextgen Scholars At Tennessee State University, Jazmine Norwood

Tennessee State University Alumni Theses and Dissertations

The underrepresentation of minority groups in food, agriculture, natural resources, and human sciences (FANHS) careers remains a challenge, limiting diversity and growth within the industry. Agriculture degrees have been disproportionately awarded to White students, while Hispanic, Black, and Native American students receive significantly fewer degrees. Research suggests that financial assistance, experiential learning, and career outreach improve minority student retention and success in FANHS. To address these challenges, the USDA’s NextGen program awarded $18.1 million to Tennessee State University (TSU) to support underserved students through scholarships, internships, and career development initiatives, including the creation of the Students Exploring Employment and Development …

Breaking The Rules: Post-Translational Modifications And Proteome Dynamics During Anoxia, Chelsea Jordan Hughes

Dissertations and Theses

Most organisms are ill-equipped to survive anoxia, but some organisms have developed molecular strategies to mitigate the cellular stressors associated with a lack of oxygen. While it is established that anoxic survival is contingent on metabolic suppression, it is unclear what mechanisms support this change in gene expression. Gene expression is regulated by a variety of mechanisms within the cell, including alteration of chromatin structure through histone modifications and protein modifications which contribute to the ever-changing proteomic landscape of the cell. Protein modifications such as ubiquitylation can lead to the degradation of proteins, while modifications to transcription factors can change …

Harnessing Electrophiles In Vivo: A Pilot Study In Swine Using A Hydrophobic Radiopaque Formulation Of 2-Propylpentanoyl Chloride, Erik Cressman, Danielle Stolley, Natalie Fowlkes, Edd Felix, Waldemar Priebe, Steve Parrish, David Fuentes

Faculty, Staff and Student Publications

Manipulating biology through chemistry is older than the discipline of chemistry itself. Traditionally, selectivity of oral or intravenous drugs relied on preferential drug-receptor binding. A novel approach exploiting image-guided techniques to impart spatial selectivity opens up a wide range of new possibilities for study and manipulation of biology. Motivated initially by the poor prognosis for solid tumors such as liver cancer, we demonstrate the use of an extreme form of in vivo chemistry for targeted delivery of 2-propylpentanoyl chloride in a swine model. The ensuing reaction in tissue devitalizes it by multiple mechanisms with lasting effects and, critically, demonstrates very …

Switching On The Evolutionary Potential Of Pancreatic Cancer: The Tumor Suppressor Functions Of Pbrm1, Luigi Perelli, Giannicola Genovese

Faculty, Staff and Student Publications

Cell plasticity is a hallmark of cancer, enabling tumor cells to acquire multiple phenotypes responsible for tumor progression, metastasis, and therapy resistance. In this issue of the JCI, Kawai and colleagues leveraged genetically engineered mouse models (GEMM) of pancreatic ductal adenocarcinoma (PDAC) to demonstrate that loss of Pbrm1, a member of the SWI/SNF complex, drives dedifferentiation and aggressive tumor features. Pbrm1 loss activated a program of epithelial-to-mesenchymal transition (EMT) and allowed the emergence of poorly differentiated histologies that are commonly associated with high recurrence rate and dismal prognosis. These findings reveal the role of the SWI/SNF complex during PDAC evolution …

Hyaluronan Network Remodeling By Zeb1 And Itih2 Enhances The Motility And Invasiveness Of Cancer Cells, Sieun Lee, Jihye Park, Seongran Cho, Eun Ju Kim, Seonyeong Oh, Younseo Lee, Sungsoo Park, Keunsoo Kang, Dong Hoon Shin, Song Yi Ko, Jonathan M Kurie, Young-Ho Ahn

Faculty, Staff and Student Publications

Hyaluronan (HA) in the extracellular matrix promotes epithelial-mesenchymal transition (EMT) and metastasis; however, the mechanism by which the HA network constructed by cancer cells regulates cancer progression and metastasis in the tumor microenvironment (TME) remains largely unknown. In this study, inter-α-trypsin inhibitor heavy chain 2 (ITIH2), an HA-binding protein, was confirmed to be secreted from mesenchymal-like lung cancer cells when cocultured with cancer-associated fibroblasts. ITIH2 expression is transcriptionally upregulated by the EMT-inducing transcription factor ZEB1, along with HA synthase 2 (HAS2), which positively correlates with ZEB1 expression. Depletion of ITIH2 and HAS2 reduced HA matrix formation and the migration and …

Drosha: A New Tumor Suppressor In Pineoblastoma, Zhixuan Huang, Xueli Ren, Jian Hu

Faculty, Staff and Student Publications

In this Outlook, Huang et al. discuss a study in this issue of Genes & Development by Fraire et al. that shows that a deficiency in miRNA processors Drosha and Dicer and consequent cell cycle gene derepression promote pineoblastoma development. The authors highlight the heterogeneity of pineoblastoma's pathogenic mechanisms and its implications for therapeutic interventions in the clinic.

Suppression Of Brca1 Facilitates Kidney Regeneration, Kaira A Church, Xunian Zhou, Raghu Kalluri

Faculty, Staff and Student Publications

Maladaptive repair following kidney injury leads to the development of kidney disease. In this issue of JEM, Ajay et al. (https://doi.org/10.1084/jem.20231107) uncover the role of breast cancer susceptibility gene 1 (BRCA1) in cell cycle arrest, DNA damage, and cell senescence, preventing maladaptive repair.