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Full-Text Articles in Life Sciences

Animal And Plant Viruses With Double-Stranded Ribonucleic Acid, Peter Gomatos Jan 1963

Animal And Plant Viruses With Double-Stranded Ribonucleic Acid, Peter Gomatos

Student Theses and Dissertations

Reoviruses occur widely in the respiratory and enteric tracts of man and animals, but their relation to disease is not clear. The present study concerns the physical-chemical characteristics and interaction with cells of reovirus type 3. A main objective of the study is to relate some of the distinguishing biological characteristics of the virus to the structure of its RNA.


Biochemical And Biological Studies On The Growth Of An Rna Containing Bacteriophage, Stephen Cooper Jan 1963

Biochemical And Biological Studies On The Growth Of An Rna Containing Bacteriophage, Stephen Cooper

Student Theses and Dissertations

The growth of the RNA-containing bacteriophage f2 has been studied. DNA synthesis appears normal after phage infection. It is possible to dissociate the synthesis of viral RNA from the synthesis of DNA using thymineless bacteria with fluorinated pyrimidines and Mitomycin C as inhibitors of DNA synthesis. It is concluded that the growth of f2 is independent of DNA synthesis.


Studies On Delayed-Type Hypersensitivity Including Attempts To Effect Transfer In Guinea Pigs With Subcellular Materials, Barry R. Bloom Jan 1963

Studies On Delayed-Type Hypersensitivity Including Attempts To Effect Transfer In Guinea Pigs With Subcellular Materials, Barry R. Bloom

Student Theses and Dissertations

The initial observation that delayed-type hypersensitivity could be transferred from an allergic to a normal animal by means of living white cells was made at The Rockefeller Institute many years ago. Since that time it has been recognized that cellular transfer appears to be a unifying principle underlying both contact dermatitis and tuberculin hypersensitivity. Later studies in man have suggested that the phenomenon can be reproduced with use cell-free extracts. Similar attempts have been made with extracts of cells from sensitized guinea pigs but, there exist only three reports in the literature which claim that transfer of contact or tuberculin …


Biochemical And Biological Studies On The Growth Of An Rna Containing Bacteriophage, Stephen Cooper Jan 1963

Biochemical And Biological Studies On The Growth Of An Rna Containing Bacteriophage, Stephen Cooper

Student Theses and Dissertations

The growth of the RNA-containing bacteriophage f2 has been studied. DNA synthesis appear normal after phage infection. It is possible to dissociate the synthesis of viral RNA from the synthesis of DNA using thymine-less bacteria with fluorinated pyrimidines and Mitomycin C as inhibitors of DNA synthesis. It is concluded that the growth of f2 is independent of DNA synthesis.


A Study Ionic Permeability Changes Underlying Excitation In Myelinated Nerve Fibers Of The Frog, Frederick Arthur Dodge Jan 1963

A Study Ionic Permeability Changes Underlying Excitation In Myelinated Nerve Fibers Of The Frog, Frederick Arthur Dodge

Student Theses and Dissertations

No abstract provided.


The Inactivation Of Neurohypophyseal Hormones By The Toad Bladder, Arthur Karlin Jan 1962

The Inactivation Of Neurohypophyseal Hormones By The Toad Bladder, Arthur Karlin

Student Theses and Dissertations

The urinary bladder of the toad, Bufo marinus, was homogenized and the particle-free supernatant fraction prepared. Incubation of this fraction with the hormones, oxytocin, arginine vasopressin, lysine vasopressin, and oxytocin ring amide caused their inactivation. The decrease in hormone activity was roughly exponential with the time of incubation.


Comparison Of The Protein Contents Of Bovine Zymogen Granules And Of Pancreatic Juice, Lewis Joel Greene Jan 1962

Comparison Of The Protein Contents Of Bovine Zymogen Granules And Of Pancreatic Juice, Lewis Joel Greene

Student Theses and Dissertations

A zymogen granule fraction has been isolated from bovine pancreas by differential centrifugation under conditions designed to favor homogeneity at the expense of yield. The fraction has been examined in the electron microscope and found to contain a limited amount of contamination represented by clearly recognizable microsomes and mitochondria. These contaminants could account for a part of the 5% phospholipid and 1% nucleic acid found in the fraction by Schneider partition. The zymogen granule fraction was composed mainly of protein, about 95%. The protein content of the granules has been solubilized by exposure to pH 8, after which a membrane …


Enzymatic Sulfurylation Mechanisms, Irving Hyman Goldberg Jan 1960

Enzymatic Sulfurylation Mechanisms, Irving Hyman Goldberg

Student Theses and Dissertations

Sulfate activation and transfer to lipids, polysaccharides, and aminoalkyl phenols have been studied In a variety of biological systems. The transfer of S3504 from radioactive 3'-phosphoadenoslne 5'-phosphosulfate (PAPS35) into lipid-soluble material has been investigated in cell-free preparations of rat brain and liver. In brain of 10-20 day old rats activity was found in the particulate fraction of the homogenate, whereas in young adult rat liver activity resided in the supernatant fraction (105,000 x g). In both systems incorporation was stimulated by the addition of a crude mixture of the isomers of N-acetyl sphingosine. Evidence accumulated …


The Biology Of A Colonial Hydroid, Chandler Montgomery Fulton Jan 1960

The Biology Of A Colonial Hydroid, Chandler Montgomery Fulton

Student Theses and Dissertations

Simple invertebrate metazoa offer many potentialities for study of growth and differentiation - potentialities which have not been realized because the organisms usually have been difficult to handle in the laboratory. A simple coelenterate, the brackish-water colonial hydroid Cordylophora lacustris, was selected for the present work, and was domesticated so that ii: it could be grown and manipulated under controlled laboratory conditions. Methods have been developed permitting exponential increase in hydranth number with a doubling time of about three days. Five ions - Na.+, K+, ca++, Mg++, c1- - have been found to be required for growth in the defined …