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A Structural And Biochemical Analysis Of The Drosophila Protein Period, Heather Anne King
A Structural And Biochemical Analysis Of The Drosophila Protein Period, Heather Anne King
Student Theses and Dissertations
Circadian clocks regulate changes in behavior and physiology that occur with a period of approximately 24 hours and are based on negative feedback loops. The molecular components of circadian clocks are conserved among animals, and a key element in all such clocks is the protein Period (PER), a circadian transcription inhibitor. The stable production, posttranslational modification, and nuclear translocation of PER all contribute to the timing of the clock. This work describes the synthesis and purification of various Drosophila PER fragments for biochemical and crystallographic analysis. Several stable PER fragments are identified, including one crystallizable fragment. The structure of the …
Integration Of Light And Temperature In The Regulation Of Circadian Gene Expression In Drosophila, Catharine E. Boothroyd
Integration Of Light And Temperature In The Regulation Of Circadian Gene Expression In Drosophila, Catharine E. Boothroyd
Student Theses and Dissertations
Clocks are aligned to the environment via inputs from both daily light and temperature cycles. Previous molecular and behavioral studies in Drosophila have largely focused on light-dependent regulation of circadian clocks and their outputs. Although light is the strongest and best understood Zeitgeber for the circadian clock, temperature is also an important factor. This thesis aims to understand better the role of temperature on gene expression and behavior in the fly, as well as to examine how information from both light and temperature are integrated by the clock to regulate circadian gene expression. Genome-wide expression profiles of transcripts from wild-type, …
Genetic And Biochemical Studies On Protein Phosphorylation In The Circadian Clock Of Drosophila Melanogaster, Saul Kivimae
Genetic And Biochemical Studies On Protein Phosphorylation In The Circadian Clock Of Drosophila Melanogaster, Saul Kivimae
Student Theses and Dissertations
Circadian rhythms in physiology and behavior are observed in almost all phyla. Genetically encoded internal clocks generate such rhythms. Identification of gene products required for the generation and maintenance of endogenous circadian near 24-hr rhythms has led to a paradigm of multiple interlocked transcriptional/translational feedback loops as the basis for molecular circadian oscillators in all studied model systems. Protein phosphorylation plays an essential role, regulating the stability, activity and subcellular localization of proteins that constitute the biological clock. This study investigates the role of the protein kinase Doubletime, a Drosophila ortholog of casein kinase Is, in the fruit fly circadian …