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Full-Text Articles in Life Sciences
A Proteomic And Genomic Investigation Into Replication Fork Dynamics In Saccharomyces Cerevisiae, Matthew David Sekedat
A Proteomic And Genomic Investigation Into Replication Fork Dynamics In Saccharomyces Cerevisiae, Matthew David Sekedat
Student Theses and Dissertations
In eukaryotic organisms, each chromosome must be precisely replicated every time a cell divides so that the genetic material can be passed on to the cell’s progeny. The work presented here is an in-depth investigation into the dynamics of the proteins that associate with progressing replication forks in yeast. A focused proteomics approach is employed to specifically identify interactions between the replication fork-coupled GINS complex and other components of the replication machinery. The scope of this technique is extended by applying it to cells that have been synchronized within the cell cycle – revealing the cell cycle dependent interactions of …
Cell Size Control And Asymmetric Cell Fates In Start Of The Saccharomyces Cerevisiae Cell Cycle, Talia, Stefano Di
Cell Size Control And Asymmetric Cell Fates In Start Of The Saccharomyces Cerevisiae Cell Cycle, Talia, Stefano Di
Student Theses and Dissertations
Understanding the molecular and biophysical mechanisms that couple the process of cell growth to cell division is one of the major challenges of modern cell biology. Saccharomyces cerevisiae (budding yeast) has been an important model organism to study the coupling between cell growth and cell division. The insights obtained from studies of this unicellular organism have been pivotal for related studies in animal systems. The classical picture that emerged from studies in budding yeast was that cell cycle commitment in G1, at a point called Start, requires growth to a critical cell size. This deterministic model did not address how …
Peak Mitotic Cyclin Permits Mitotic Exit, Benjamin Drapkin
Peak Mitotic Cyclin Permits Mitotic Exit, Benjamin Drapkin
Student Theses and Dissertations
In eukaryotes, DNA replication, mitosis, and cytokinesis are all regulated by Cyclin-dependent kinase (Cdk). Cyclin/Cdk complexes promote replication origin firing and mitotic entry, and conversely, inhibit pre-replication origin loading and exit from mitosis. Cyclin synthesis and degradation, Cdk phosphorylation and Cdk inhibitors are controlled such that Cdk activity oscillates once per cell cycle. Little is known about the quantitative relationship between the level of Cdk activity and the occurrence, rate, and coordination of cell cycle events. We have addressed this question in Saccharomyces cerevisiae by introducing titrated levels of undegradable mitotic B-cyclin (Clb2kd) in cells prior to release of a …