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Full-Text Articles in Life Sciences
Functional Roles Of Nova In Rna Metabolism, Giovanni Stefani
Functional Roles Of Nova In Rna Metabolism, Giovanni Stefani
Student Theses and Dissertations
Nova-1 is a neuron-specific RNA binding protein, distributed both in the nucleus and the cytoplasm of several populations of neurons. The work presented here confirms that Nova-1 binds a sequence of RNA found in an intron of the glycine receptor a2 subunit pre-mRNA. Evidence that Nova-1 acts as a regulator of alternative splicing in transfected cell lines is presented. Furthermore, a direct role of Nova-1 on splicing is demonstrated by establishing an in vitro assay for Nova-l's regulatory role in splicing. Potential functional partners of Nova are suggested by the demonstration of physical interactions between Nova-1 and molecules whose action …
Subcellular Localization And Activity Of The Multidrug Resistance Protein 1, Asha Rajagopal
Subcellular Localization And Activity Of The Multidrug Resistance Protein 1, Asha Rajagopal
Student Theses and Dissertations
Multi-drug resistance or MDR is a major impediment to the successful administration of chemotherapy. Broadly defined, the term MDR applies to any mechanism the cell uses to counter the effects of chemotherapeutic agents, protecting the cell at once against the toxicity of many, structurally dissimilar compounds. There are several routes to MDR for a cell, and they include everything from decreasing intracellular drug concentrations to increasing rates drug metabolism. The study presented here focuses on the role of drug transporters in conferring drug resistance. MDR transporters are traditionally thought to extrude cytotoxins from the cell at the plasma membrane, and …
Measurement Of The Diffractive Structure Function Of The Antiproton In Proton-Antiproton Collisions At √ S = 1800 And 630 Gev, Kenichi Hatakeyama
Measurement Of The Diffractive Structure Function Of The Antiproton In Proton-Antiproton Collisions At √ S = 1800 And 630 Gev, Kenichi Hatakeyama
Student Theses and Dissertations
A measurement of the effective diffractive structure function Ffi of the antiproton obtained from a study of single diffractive dijet events produced in association with a leading antiproton in pp collisions at the center-of-mass energy y/s = 1800 and 630 GeV is presented. Inclusive samples of single diffractive events were collected during the Tevatron collider run of 1995—1996 using the Collider Detector at Fermilab (CDF) by triggering on a leading antiproton detected in a forward Roman Pot spectrometer. From these samples, single diffractive dijet subsamples were selected by requiring two or more jets with transverse energy ET > 7 GeV in …
Protein Semi-Synthesis In Vivo, Izabela Giriat Stankiewicz
Protein Semi-Synthesis In Vivo, Izabela Giriat Stankiewicz
Student Theses and Dissertations
Incorporation of chemical probes into proteins is a powerful way to elucidate biological processes and to engineer novel function. This thesis describes the ligation of synthetic molecules to target proteins in an intracellular environment, and the use of semi-synthetic proteins for whole animal studies. In the first approach a cellular protein is genetically tagged with one half of a split-intein. The complementary half is linked in vitro to the synthetic probe and this fusion is delivered into cells using a transduction peptide. Association of the intein halves in the cytosol triggers protein transsplicing, resulting in the ligation of the probe …
Isolation And Characterization Of Estrogen Receptor- Interacting Proteins, Yun Kyoung Kang
Isolation And Characterization Of Estrogen Receptor- Interacting Proteins, Yun Kyoung Kang
Student Theses and Dissertations
Estrogen fulfills a variety of physiological roles through its nuclear receptors, estrogen receptors (ERs). The action of ERs is modulated and mediated by diverse interacting cofactors. The initial aim of this thesis work was to identify ligand-, subtype- and/or cell typespecific ER-interacting proteins. I identified a number of nuclear extract-derived proteins that interact with immobilized ER ligand binding domains (LBDs) in a 17(3-estradiol (E2)-dependent manner. The most prominent of these are components of the thyroid hormone receptor associated protein (TRAP)/Mediator coactivator complex, which interacts with ERa and ER(3 both in unfractionated nuclear extracts and in purified form equally efficiently. Although …
Rck Domain Model Of Calcium Activation In Bk Channels, Alexander R. Pico
Rck Domain Model Of Calcium Activation In Bk Channels, Alexander R. Pico
Student Theses and Dissertations
Potassium ion channels are ubiquitously expressed from bacteria to mammals where they are involved in various processes ranging from the regulation of osmotic pressure in a single cell to the electrical response in muscle fibers to the generation of action potentials in neurons. The B K channel family (BK for Big K+ conductance) is an interesting subfamily of K+ channels responsive to both membrane voltage and intracellular calcium ion. The unique, high-affinity Ca2+ sensitivity of B K channels is critical to their physiological function in various cell types. The mechanism by which Ca2+ activates B K channel gating, however, is …
Identification And Characterization Of The Dna Damage Response Function Of Human Rif1 And A Search For Human Rap1 Interacting Factors, Joshua Silverman
Identification And Characterization Of The Dna Damage Response Function Of Human Rif1 And A Search For Human Rap1 Interacting Factors, Joshua Silverman
Student Theses and Dissertations
Rifl, the ortholog of the yeast Rapl interacting factor 1, a telomere length regulator was identified. Immunofluorescence did not reveal an association of human Rifl with telomeres. Preliminary co-immunoprecipitation with known telomeric proteins and chromatin immunoprecipitation failed to show an interaction of human Rifl with telomeric protein complexes or with telomeric DNA. Unexpectedly, Rifl responded to ionizing radiation (IR), UV light, and clastogens, forming foci that co-localized with other DNA damage response factors such as 53BP1, ATM, BRCA1, Chkl, Nbsl, and Rad 17. Furthermore, Rifl localized to uncapped telomeres, as do other DNA damage response factors. Among DNA damage response …