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The Multifunctional Capsid Gene Of Hepatitis C Virus, Laura Katherine Mcmullan
The Multifunctional Capsid Gene Of Hepatitis C Virus, Laura Katherine Mcmullan
Student Theses and Dissertations
In populations worldwide, the Hepatitis C Virus displays great diversity. The capsid gene, however, is remarkably conserved and this has led to predictions of RNA secondary structure and an overlapping, alternative reading frame (ARF). We investigated a role of the ARF in HCV infection, by introducing four stop codons into the ARF of a genotype 1a H77 molecular clone. These changes did not alter the capsid protein sequence, but were predicted to disrupt the RNA secondary structures, SLV and SLVI. An infection was launched after inoculation of the mutant HCV RNA into an HCV naïve chimpanzee. The acute infection was …
Genetic And Biochemical Analyses Of The Flaviviridae Capsid Proteins, Catherine Lucy Murray
Genetic And Biochemical Analyses Of The Flaviviridae Capsid Proteins, Catherine Lucy Murray
Student Theses and Dissertations
The small, enveloped viruses of the family Flaviviridae are etiological agents of numerous important human and agricultural diseases including hepatitis C, yellow fever, and bovine viral diarrhea. Efficient dissemination of these viruses is dependent on the production of infectious particles, thought to arise by budding of the capsid protein and associated genomic RNA through a host cell-derived lipid membrane outfitted with envelope glycoproteins. The process of virion morphogenesis is not well understood, but the presumed involvement of numerous viral and cellular components makes it an attractive target for novel therapeutic drug design. To investigate the early events of Flaviviridae particle …