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Theses/Dissertations

Rockefeller University

Cholesterol

Publication Year

Articles 1 - 3 of 3

Full-Text Articles in Life Sciences

Bioorthogonal Chemical Reporters Reveal Fatty-Acylation Of Histone H3 Variants And Cholesterol Modification Of Proteins And Trafficking In Cells, John P. Wilson Jan 2011

Bioorthogonal Chemical Reporters Reveal Fatty-Acylation Of Histone H3 Variants And Cholesterol Modification Of Proteins And Trafficking In Cells, John P. Wilson

Student Theses and Dissertations

Lipids are essential components to all known life. They serve many functions from basic building blocks to covalent protein modification and are particularly involved in signaling. Many rare and widespread human health concerns involve lipids but despite their importance, their analysis either free or protein-bound has remained difficult for decades. Here, using bioorthogonal chemistry, we have developed a robust system to identify the proteins modified by lipids and advanced the ability to image cholesterol in cells and tissues. The unbiased proteomic analysis of fatty-acylated proteins using chemical reporters undertaken here revealed a greater diversity of lipid-modified proteins in mammalian cells …


Characterization Of The Functional Role Of The Intracellular Cholesterol Transporter Stard4 In Knockout Mice, And Investigation Of Epigenetic Modulation Of Apoa-I Transcription, Joshua Riegelhaupt Jan 2010

Characterization Of The Functional Role Of The Intracellular Cholesterol Transporter Stard4 In Knockout Mice, And Investigation Of Epigenetic Modulation Of Apoa-I Transcription, Joshua Riegelhaupt

Student Theses and Dissertations

Cholesterol is crucial for mammalian survival by playing important roles, such as regulating membrane fluidity and as a precursor for the synthesis of steroid and sex hormones, bile acids, and Vitamin D. In addition, cellular and organismal regulation of cholesterol is important for health. For example, increased levels of plasma LDL cholesterol are a risk factor for coronary heart disease and stroke. Intracellular cholesterol levels are regulated by a variety of mechanisms, but numerous studies indicate a very important role for transcriptional regulation by Sterol Regulatory Binding Proteins (SREBPs), Liver X Receptors (LXRs) and Unfolded Protein Response (UPR) or ER …


A Novel Subfamily Of Three Star-Related Lipid Transfer Proteins That Are Differentially-Regulated And Function In Intracellular Cholesterol Metabolism, Raymond E. Soccio Jan 2004

A Novel Subfamily Of Three Star-Related Lipid Transfer Proteins That Are Differentially-Regulated And Function In Intracellular Cholesterol Metabolism, Raymond E. Soccio

Student Theses and Dissertations

thesis describes the discovery, cloning, and initial characterization of StarD4, sterol-regulated gene encoding a StAR-related lipid transfer (START) protein, and its close homologues, StarD5 and StarD6. StarD4 was identified using cDNA microarrays, as liver StarD4 expression decreased three-fold in mice fed a high cholesterol diet. StarD4 also sterol-regulated in cultured cells, and a functional sterol regulatory element (SRE) identified in its promoter. StarD4 was preferentially activated in mouse liver by SREBP-rather than SREBP-1, supporting a role in cholesterol rather than fatty acid metabolism. X-ray crystal structure of StarD4 was solved, revealing a hydrophobic lipid binding cavity described for other START …