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Characterization Of A Novel Chromatinâ€Induced Mechanism That Couples Microtubule Disassembly And Nuclear Reâ€Formation, Eileen Madeleine Woo
Characterization Of A Novel Chromatinâ€Induced Mechanism That Couples Microtubule Disassembly And Nuclear Reâ€Formation, Eileen Madeleine Woo
Student Theses and Dissertations
Upon completion of mitosis, the disassembly of spindle components and reassembly of nuclear structures occur simultaneously around chromatin. Previous studies have suggested that an important step in this process is the inactivation of the Aurora B kinase by the Triple Aâ€ATPase Cdc48/p97, which physically extracts the protein from chromatin at anaphase. Aurora B is the catalytic subunit of the Chromosome Passenger Complex (CPC), which promotes microtubule polymerization and spindle formation from mitotic chromosomes. Removal of the CPC from chromosomes at anaphase is required for proper nuclear reassembly, but the molecular basis for this requirement remains unclear. On the whole, the …
Regulation Of Chromosome Segregation By The Chromosomal Passenger Complex, Boo Shan Tseng
Regulation Of Chromosome Segregation By The Chromosomal Passenger Complex, Boo Shan Tseng
Student Theses and Dissertations
Life depends on faithful DNA segregation. The molecular underpinnings controlling this segregation are not fully understood. Here I describe the role of the chromosomal passenger complex (CPC) in the regulation of chromosome segregation, using Xenopus egg extracts. The CPC contains the kinase Aurora B, which is well known for its phosphorylation of serine 10 on histone H3 (H3S10). While this phosphorylation is a hallmark of M-phase, its functional significance is enigmatic. In the first part of my thesis, I, with my collaborators, provide a molecular function for this phosphorylation in the chromosomal dissociation of HP1 (heterochromatin protein 1) in M-phase. …
Control Of Chromosome Segregation By The Aurora B Complex, Srinath Cidambi Sampath
Control Of Chromosome Segregation By The Aurora B Complex, Srinath Cidambi Sampath
Student Theses and Dissertations
Chromosome segregation during cell division requires spindle assembly around M-phase chromatin. In cells lacking centrosomes, such as those found in female meiosis, chromosomes themselves nucleate and stabilize microtubules in order to promote accurate spindle formation. Here we present a description of the composition and function of the vertebrate chromosomal passenger complex (CPC), known to include Incenp, Survivin, and the kinase Aurora B. We report the identification of Dasra A and Dasra B as two new components of the vertebrate CPC, and demonstrate that the CPC is required for chromatin-dependent spindle formation in Xenopus egg extracts. The failure of microtubule stabilization …