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Full-Text Articles in Life Sciences
Tumor Cell Glutathione Metabolism Therapeutic Implications, Bradley Alan Arrick
Tumor Cell Glutathione Metabolism Therapeutic Implications, Bradley Alan Arrick
Student Theses and Dissertations
In response to oxidative injury murine tumors depend upon the GSH oxidation-reduction cycle. The susceptibility of tumor cells to lysis by a flux of H2O2, such as generated by granulocytes or activated macrophages, or by the enzyme glucose oxidase, was augmented by interference with the GSH redox cycle. Depletion of tumor cell GSH was by 2 methods: incubation of cells with buthionine sulfoximine (BSO), a nontoxic inhibitor of GSH synthesis, or with l-chloro-2,4-dinitrobenzene_ a substrate for GSH S-transferase. Sensitization to oxidative cytolysis correlated with GSH depletion by either method by the critera of both dose-response and …
Structure-Function Relationships Of Elements Of The Bacteriophage F1 Genome, Phyllis Barbara Moses
Structure-Function Relationships Of Elements Of The Bacteriophage F1 Genome, Phyllis Barbara Moses
Student Theses and Dissertations
Expression of the ten genes on the small filamentous phage f1 genome must be closely regulated during the infection cycle, since this nonlytic coliphage lives in delicate balance with its host. The sequence and context requirements of specific regions involved in f1 mRNA metabolism were investigated by: 1) relocation of an intact transcription unit on the phage genome; 2) construction of a hybrid E. coli-f1 transcription unit which could be studied in a phage or plasmid context; 3) identification and characterization of a rho-dependent transcription termination signal in f1 by cloning it between the promoter and gene of an E. …
Sequence And Functional Structures Of The Extrachromosomal Rdna Minichromosome Of Physrum Polycephalum, Gerald Ray Campbell
Sequence And Functional Structures Of The Extrachromosomal Rdna Minichromosome Of Physrum Polycephalum, Gerald Ray Campbell
Student Theses and Dissertations
The ribosomal RNA genes of Physarum polycephalum are encoded on multiple copies of linear, palindromic extrachromosomal DNA molecules (rDNA), which have been isolated both as a satellite DNA molecule, and as a "minichromosome" with associated chromatin proteins. The genes coding for the 19 S, 26 S, and 5.8 S rRNAs are transcribed as a single, large precursor molecule, beginning nearer the center of the palindrome and transcribing outward. The genes are in the order 19 - 5.8 - 26, and the 26 S gene contains two intervening sequences. The intervening sequences are transcribed, and later excised out. The region of …
A Transmembrane Precursor Of Secretory Component, Keith Elliot Mostov
A Transmembrane Precursor Of Secretory Component, Keith Elliot Mostov
Student Theses and Dissertations
Secretory component (SC), a glycoprotein associated with polymeric IgA and IgM (pIg) in external secretions, is produced by certain epithelial cells and is thought to be the receptor mediating the transcellular transport of pIg. We studied the biosynthesis and processing of rabbit and human SC. Using translation of mRNA from rabbit mammary gland and liver in a cell-free system supplemented with dog pancreas microsomal vesicles, we discovered that the translation products of rabbit SC include at least four polypeptides. Moreover, we found that all four polypeptides are synthesized not as soluble secretory forms, but as larger transmembrane forms that are …
Comparative Biochemistry Of Drug-Binding Proteins In Calmodulin/S100 Family, Daniel Robert Marshak
Comparative Biochemistry Of Drug-Binding Proteins In Calmodulin/S100 Family, Daniel Robert Marshak
Student Theses and Dissertations
Calmodulin is a member of a class of structurally and functionally related proteins. Calmodulin is widely distributed among eukaryotes, and the amino acid sequence of calmodulin is highly conserved. The protein contains multiple structural domains and has multiple activities in vitro, including calcium dependent drug binding. In the studies presented in this thesis, we have used phenothiazines as tools to probe the relationships between structural domains and functional domains in calmodulin. The approach to the problem of structure-function relationships can be framed in three questions: Do other calciurn modulated proteins show calcium dependent drug binding activity? What are the structural …
Genetic And Biochemical Analysis Of The Bryan Strain Of Rous Sarcoma Virus, Teena Louise Lerner
Genetic And Biochemical Analysis Of The Bryan Strain Of Rous Sarcoma Virus, Teena Louise Lerner
Student Theses and Dissertations
Integration of Rous sarcoma virus DNA into its host genome was analyzed under conditions where secondary integration via virus spread was inhibited. This was accomplished by using the noninfectious pol- ,env- alpha variant of the Bryan high titer strain of Rous sarcoma virus (BH-RSV). Twelve independent BHRSV- transformed chicken embryo fibroblast clones were obtained and the provirus-cell junction fragments were mapped by restriction endonuclease cleavage and Southern blotting analyses. We found that expression of the viral genes could occur after proviral integration at many sites on the chicken genome and that there was no apparent preference for specific …
Liver Cell Adhesion Molecules In Embryonic Development, Warren J. Gallin
Liver Cell Adhesion Molecules In Embryonic Development, Warren J. Gallin
Student Theses and Dissertations
Cell-cell adhesion is one of the essential processes for normal embryogenesis. The isolation and characterization of the neural cell adhesion molecule, N-CAM, provided the first substantial opportunity to study this process at a molecular level. Moreover, studies of N-CAM expression in embryos suggested that cell adhesion molecules may play a critical role in induction. This thesis describes the isolation of a glycoprotein, L-CAM, that mediates adhesion between liver cells, using an assay similar to the one used to isolate N-CAM.