Laboratory and Basic Science Research Commons^™

Influence Of Time Spent In Captivity On Aggressive Behaviors Of Gray Wolves Canis Lupus, Chelsea Knutson

Journal of Earth and Life Science

Knowledge of the characteristics that promote group success is key to understanding the adaptive advantages of sociality for many group-oriented species. In this study, nine Gray Wolves Canis lupis were each observed twelve times over a period of six months to determine if the amount of time spent in the wild/captivity had any effect on aggressive behaviors. The number of aggressive behaviors were recorded for each wolf during each observation. The nine wolves were separated into three groups depending on their upbringing; born and raised in captivity, born in the wild and raised in captivity, and born and raised in …

Low Cost, Multi-Purpose Genotyping Panels For Dairy And Beef Cattle, Michelle Judge

Theses

rhe selection of animals based on DNA has revolutionised animal breeding, but the associated high cost of obtaining genotypes has limited its uptake. The objective of this thesis was to develop a low-cost, low-density, multi-purpose genotyping panel for the procurement of reliable genotype information, and to quantify the long-term consequences of using such low-density genotype panels in breeding programs. The objectives were achieved through a combination of real-life cattle genotype data and simulations to mimic a cattle population. The in-silico development of genotype panels was based on actual genotypes from up to 58,705 beef cattle. Alternative novel strategies were used …

A Comparison Of Benthic Macroinvertebrate Assemblages Between Perennial And Intermittent Headwater Streams Of The Mattole River In Northern California, Usa, Mason S. London

Cal Poly Humboldt theses and projects

Intermittent streams are common throughout the world and comprise 60% or more of total river lengths in the conterminous United States. Despite their prevalence, intermittent streams are understudied, particularly first-order headwater streams, which are vital for maintaining the function, health and biotic diversity of river networks. In June 2016, I sampled five intermittent and five perennial headwater streams in the Mattole River watershed in northwestern coastal California, USA, to compare benthic macroinvertebrate (BMI) assemblages between intermittent and perennial streams. BMI samples were collected using a 500µm mesh D-net at eight randomly located riffles along a 150-m reach, and then composited, …

Planaria: An Animal Model That Integrates Development, Regeneration And Pharmacology, Oné R. Pagán

Biology Faculty Publications

Although planarians are established model organisms in developmental biology and regeneration studies, in the last forty years or so, they have caught the attention of pharmacologists, especially to study the pharmacology of drugs of abuse. This review covers the following topics: some fundamentals of the history of animal models and planarians in biomedical research; an abbreviated story of systematic pharmacology research using planarians as a model organism; an example of how planarians are contributing to the search for compounds against acute cocaine toxicity; an analysis of the number of papers on planarians and pharmacological topics from 1900- 2016; some perspectives …

Ionic Regulation Of Critical Cellular Processes In Non-Excitable Cells, Brandon M. Franklin

Theses and Dissertations--Biology

There are long-standing hypotheses that endogenous ion currents act to control cell dynamics in development, wound healing and regeneration. However, the mechanisms employed by cells to detect the electric field (EF) and translate it into a discernable message to drive specific cell behaviors, such as migration, proliferation and differentiation, are not well understood. A better understanding of how cells are able to sense EFs and react to them is vital to understanding physiological mechanisms are involved in regeneration. Ion channel signaling provides a reasonable suspect for mediating these effects based on their documented involvement in proliferation, migration and differentiation.

To …

Evolution Of Equine Arteritis Virus During Persistent Infection In The Reproductive Tract Of The Stallion And The Male Donkey, Bora Nam

Theses and Dissertations--Veterinary Science

Equine arteritis virus (EAV) establishes persistent infection in the stallion reproductive tract, and the carrier stallion continues to shed virus in semen for weeks to years or lifelong. The objective of this study was to elucidate the intra-host evolution of EAV during persistent infection in stallions. Seven EAV seronegative stallions were experimentally infected with EAV KY84 strain and followed for 726 days post-infection, and sequential clinical samples including semen were collected for virus isolation and next-generation sequencing (NGS). In addition, archived sequential semen samples from two stallions that were naturally infected with EAV KY84 for a long-period (up to 10 …

Correction For Munson And Carroll, “What’S In A Name? New Bacterial Species And Changes To Taxonomic Status From 2012 Through 2015”, Erik Munson, Karen C. Carroll

Clinical Lab Sciences Faculty Research and Publications

Author Correction.

Burbot Growth Rates In A Northern Mesotrophic Lake, Samuel J. Peterson

Journal of Earth and Life Science

Burbot Lota lota has a circumpolar distribution throughout cold-water areas and is the only freshwater member of the family Gadidae. There are currently very few management techniques applied to Burbot throughout their northern extent. Growth is an indicator of fish health, biomass, and habitat quality; therefore, the objective of this study was to determine the growth rate of Burbot in Lake Bemidji. Burbot (n = 45) were collected along three sites on Lake Bemidji for years 2015-2017. Age was determined from sagittal otoliths, the marginal zones of which were opaque from December to May and translucent from June to November. …

Effects Of Catch-And-Release Angling On Egg Densities In Largemouth Bass Nests, Tyler Robinson

Journal of Earth and Life Science

Predation during early life stages of Centrarchidae offspring are a major cause of decreased reproductive success. For five-weeks Largemouth Bass Micropterus salmoides defend their broods from invaders such as Yellow Perch Perca flavescens and Bluegill Lepomis macrochirus. The focus of this study was to determine if predation upon Largemouth Bass broods increased as removal time from the nest via catch-and-release angling increased. Sampling was conducted on the Whitefish Chain, MN from 7 May - 7 June 2016, where a total of 29 nests were located and sampled. Three treatment groups were used; immediate catch-and-release, three-minute, and a seven- minute …

Effect Of Abiotic Factors On Bluegill Size Structure In Nine Central Minnesota Lakes, Ryan Henry

Journal of Earth and Life Science

Bluegill are the most targeted species of fish in Minnesota. In recent years, the abundance of slow growing, small Bluegill has increased within the state. Angler harvest of Bluegill can reduce the average length of Bluegill up to four times the original length. Abiotic factors such as maximum depth, average water clarity, littoral area, and percent littoral area can influence the dynamics of a lake system. There has been limited research on whether these abiotic factors can influence the size structure of a Bluegill population. The objective of this study is to determine if there is a direct relationship between …

Po2 Dependence Of Oxygen Consumption In Skeletal Muscle Of Hypertensive And Normotensive Rats, Habiba Shah

Theses and Dissertations

Human essential hypertension affects over 75 million people in the United States, and can lead to death due to its several serious health complications such as hypertension-related cardiovascular disease. The purpose of this research was to understand how hypertension could cause physiological changes to the microcirculation, specifically the PO₂ dependence of oxygen consumption (VO₂) in skeletal muscle of normotensive and hypertensive rats. The Spontaneously Hypertensive Rat (SHR) strain was used as the diseased model, and Wistar-Kyoto (WKY) rats were used as controls to conduct this study. The SHR strain develops hypertension between 5-6 weeks after birth with …

Using Extremophile Bacteriophage Discovery In A Stem Education Professional Development Partnership To Explore Model Classroom Research Experiences Integrating The Three Dimentions Of The Next Generation Science Standards, Carrie L. Boudreau Ms

All Student Scholarship

The National Research Council’s (NRC) A Framework for K-12 Science Education: Practices, Crosscutting Concepts, and Core Ideas describes a vision of what it means to be proficient in science. The project discussed in this thesis was developed with a NIH SEPA Grant 8R25OD010937 to the Virology and Electron Microscopy Laboratory at the University of Southern Maine (USM) under the direction of Dr. S. Monroe Duboise. The goal of the project was to explore using discovery of extreme environment bacteria and their bacteriophages as a model for using the three dimensions of learning to teach Next Generation Science Standards (NGSS). Specifically, …

Are Weevils Picky Eaters? Community Structure And Host Specificity Of Neotropical Saproxylic Beetles (Coleoptera: Curculionidae), Jhunior A. Morillo

Dissertations and Theses

Abstract Primary saproxylic beetles play a major role in forest nutrient cycling and making deadwood accessible to other decomposers. Understanding beetle host preferences and patterns of community assembly is critical for their conservation, and for predicting which species might become invasive. This project aims to investigate the ecological and host specificity, as well as the community composition of curculionids in a mosaic of old-growth (OG) and secondary forest on the Osa Peninsula, Costa Rica. The subfamily Scolytinae was expected to be the most species-rich and abundant. Ambrosia beetles were expected to have more generalist species than other curculionids. Old growth …

The Reliability Of The Navicular Drop Test And Its Transferability To Dynamic Movement, Joshua Krispin

College of Graduate Studies: Theses & Dissertations

Background: Navicular drop can be defined as the distance the navicular tuberosity moves from a standing weight bearing to a standing neutral position, as the subtalar joint moves from a relaxed position to a neutral position. Navicular drop is an important measurement for clinicians used to describe foot function, pronation, and excessive movement seen in several pathologies. Objective: The purpose of this study is to see if navicular drop is influenced by mode or speed of locomotion, if it is will there be other influences such as the forefoot and heel soft tissue, and will those factors influence those measures. …

Sex Differences In Chronic Stress Effects On Cognition In Rodents, Victoria N. Luine, Juan Gomez, Kevin D. Beck, Rachel E. Bowman

Psychology Faculty Publications

Chronic stress causes deleterious changes in physiological function in systems ranging from neural cells in culture to laboratory rodents, sub-human primates and humans. It is notable, however, that the vast majority of research in this area has been conducted in males. In this review, we provide information about chronic stress effects on cognition in female rodents and contrast it with responses in male rodents. In general, females show cognitive resilience to chronic stressors which impair male cognitive function using spatial tasks including the radial arm maze, radial arm water maze, Morris water maze, Y-maze and object placement. Moreover, stress often …

A Study Of Critical Value Notification In The Outpatient Setting: The Relationship Between Physician Response And Patient Outcomes, Kristie Renee Finney

Theses and Dissertations

Critical values are laboratory values that represent a life-threatening condition for which there is a treatment available. Laboratories make immediate notifications to ordering providers when critical values are identified so that they may quickly act to initiate a treatment for their patient. The majority of laboratories apply the inpatient critical value list to the outpatient setting, although there are many differences between an acutely ill inpatient population and an ambulatory outpatient population. The goal of this study was to determine if providers responded to the critical values in the outpatient setting and to determine if there was a difference in …

Efficient Method For Transfer Of Microinjected Eggs To Mouse Ampulla For Generating Transgenic Mice, Guang Wen, Jin Di, Qian Li, Jianling Chen, Ling Jin, Cheng Wang, Sanqing Xu

Publications and Research

Background: The new method described here is highly efficient in transferring microinjected mouse eggs (MEs) through the bursa membrane of a surrogate mother mouse to the ampulla of the oviduct without damaging the blood vessels on the bursa membrane.

Results: This method causes no loss of blood, and it produces newborn pups/founders from approximately 70% of the transferred MEs, because only a small hole is made on the blood vessel–free area of the bursa membrane and ampulla of the surrogate mother mouse. The infundibulum remains intact. The small hole on the bursa membrane/ ampulla may already heal up before the …

Examining The Combined Effects Of Dissolved Oxygen, Temperature, And Body Size On The Physiological Responses Of A Model Macrobenthic Polychaete Species, Capitella Teleta, Kelsey Burns Gillam

Dissertations

While the scientific community is in consensus that coastal systems are threatened by climate change, few climate change studies test the effects of more than one variable directly related to climate change. The dissolved oxygen (DO) levels of the ocean are currently subject to both global warming and eutrophication; 94% of all hypoxia zones are expected to experience >2°C increase by 2035. This dissertation aims to examine how a model organism responds to simultaneous thermal and DO stress involving four levels of DO (100%, 70%, 50%, and 20%) saturation and three temperatures (15°C, 20°C, and 25°C).

The polychaete, Capitella teleta …

Punctuated Evolution Within A Eurythermic Genus (Mesenchytraeus) Of Segmented Worms: Genetic Modification Of The Glacier Ice Worm F1f0 Atp Synthase, Shirley A. Lang

Graduate School of Biomedical Sciences Theses and Dissertations

Segmented worms (Annelida) are among the most successful animal inhabitants of extreme environments worldwide. An unusual group of Mesenchytraeus worms endemic to the Pacific Northwest of North America occupy geographically proximal ecozones ranging from low elevation temperate rainforests to high altitude glaciers. Along this altitudinal transect, Mesenchytraeus representatives from disparate habitat types were collected and subjected to deep mitochondrial and nuclear phylogenetic analyses. Evidence presented here employing modern bioinformatic analyses (i.e., maximum likelihood, Bayesian inference, multi-species coalescent) supports a Mesenchytraeus “explosion” in the upper Miocene (5-10 million years ago) that gave rise to ice, snow and terrestrial worms, derived from …

Targeting Estrogen Receptor Negative Breast Cancer Cells Using Diarylthiourea Analogs Of Sheta2, Hongye Zou

Dissertations, Masters Theses, Capstones, and Culminating Projects

Abstract will available after document embargo ends.