Entomology Commons^™

Development Of Host-Selective, Pcr-Inhibiting Oligos As A Tool To Investigate The Eukaryotic Symbionts Of Insect Hosts, Christian L. Weinrich

2023 Midwest Ecology & Evolution Conference

Insects are hosts to diverse communities of other organisms. These communities can include microbes such as bacteria, yeast, and protists, as well as multicellular organisms such as nematodes and mites. The prokaryotic associates of many insects have been well-studied and are often investigated by amplifying and sequencing the gene for the 16S ribosomal subunit using universal primers.

However, the eukaryotic associates of many insects remain poorly characterized, and this is in part due to the difficulty in utilizing 18S (the eukaryotic analog for 16S) for eukaryote detection. Because the insect host is also a eukaryote and makes up the bulk …

Everything But The Lab Sink: Using 1,000 Phylogenomically Informative Markers To Resolve Historically Messy Species Groups In Anastrepha Fruit Flies (Diptera: Tephritidae), Kristie J. Schmidt

2023 Midwest Ecology & Evolution Conference

The genus Anastrepha (Diptera: Tephritidae) is long overdue for a major taxonomic revision. Of the 22 recognized species groups within Anastrepha, only ~5 are well supported clades. Molecular methods, such as traditionally used mitochondrial DNA markers, have been unsuccessful at elucidating relationships amongst Anastrepha species; therefore, a more robust phylogenomic tool is likely necessary. To accomplish this, we used Highly Multiplexed Amplicon-based Phylogenomics (HiMAP) to identify 1,000 phylogenomically informative nuclear markers. Post sequencing, we obtained data for 500+ amplicons for 90 Anastrepha spp. belonging to 18 Anastrepha species groups. Our findings agree with some aspects of the most recently …

Effect Of Trap Type And Lure Presence On Diversity Of Jewel Beetles (Coleoptera: Buprestidae) Captured, Maddie Bidinger

2023 Midwest Ecology & Evolution Conference

Woodboring beetles in the family Buprestidae pose a major threat to forest ecosystems. The larvae directly damage host trees by feeding, and may also weaken hosts, making them susceptible to pathogens or physical damage. Moreover, individuals of this taxa are readily transported through international commerce, creating a high risk of establishment outside of their native geographical ranges. For this reason, survey programs use traps to monitor for these and other woodboring beetles. Several different trap designs are typically deployed during surveys because a single trap design hasn’t been demonstrated as equally effective for all taxa. Thus, there is strong interest …

Biting The Hand That Feeds: Food Density, Wing-Length And Fecundity In Aedes Atropalpus, Ellie Armstrong

2023 Midwest Ecology & Evolution Conference

Wing length has been shown to correlate to fecundity in mosquitoes. By measuring wing length and counting eggs, one can create a linear equation that can be used to calculate fecundity using wing length. A 2008 equation by Armistead that looked at wing length fecundity relationship in Ae. atropalpus only had samples over 2.4 mm. Because of this, any wing length under 2.4 that is put in the equation will have a fecundity less than zero. This study was created to replicate the Armistead study with smaller mosquitoes to create an equation that calculates the fecundity of smaller mosquitoes. To …

Does The Genome Of A Putatively Specialist Moth (Schinia Gracilenta) Contain A Signal Of The Indigenous Cultivation Of Its Host Plant (Iva Annua), Wren Renquist

2023 Midwest Ecology & Evolution Conference

When one species depends on one another for survival, they may have shared demographic histories. Further, when symbionts have shorter life spans, their rate of evolution may be quicker than that of their hosts. For this reason, detecting a recent demographic signal in a symbiont’s genome may often be easier than directly studying the host.

Here I study the slender flower moth (Schinia gracilenta) and its host plant, sumpweed (Iva annua). Sumpweed is thought to have been cultivated in North American ca. 5000 BC - AD 1700 and its range may have been expanded during this time. I collected S. …

Pollinators And Flowering Resources In Louisville Urban Green Spaces, Sarah Hobbs

2023 Midwest Ecology & Evolution Conference

Urban Greenspaces can appear in many different forms such as parks, urban orchards, and community gardens. These spaces provide important ecosystem services such as enhanced biodiversity, improved soil health, and increased carbon sequestration. Greenspaces such as parks and community gardens have been relatively well studied in the last few decades compared to urban orchards, despite the multitude of ecosystem services that can be provided or enhanced by these orchards. We hypothesize that urban orchards will provide the optimal conditions for pollinator communities because urban orchards normally have the greatest variety of vegetation and flowering resources, which is positively correlated with …

Leaf Litter Arthropod Communities Not Responsive To Nutrient Pulse Subsidies, Andrew R. Seiler, Stephen P. Yanoviak

2023 Midwest Ecology & Evolution Conference

Spatial subsidies are the asymmetric movement of resources from a donor habitat to a distinct recipient habitat. Arthropod rain, i.e., the fall of arthropods from tree crowns to the forest understory, is a potentially important subsidy link between canopy food webs and detritus-based litter food webs. We added homogenized fragments of Blaptica dubia cockroaches in high (12g m-2) and low (2g m-2) concentrations to replicated quadrats of leaf litter as a pulse subsidy in a temperate forest. Controls were: 1) zero subsidy and 2) addition of foam pellets in place of cockroach fragments. Litter in each quadrat was subsampled 2, …

Qtl Analysis Of Body Size And Development Time In Dendroctonus Ponderosae, Camille M. Pushman

2023 Midwest Ecology & Evolution Conference

The mountain pine beetle (MPB, Dendroctonus ponderosae) is a pest species found in western Norh America that attacks and kills Pinus host trees. Previous studies have identified genetic divergence between populations and variation in life-history traits (body size and development time) that are likely important in local adaptation. To understand the underlying genetic architecture of MPB adaptation and divergence, we performed an F₁ intercross between two distinct populations (Utah & Arizona) and performed a QTL analysis. We analyzed double digest RAD sequencing data from 181 F2 individuals. Our final dataset consists of 986 SNPs and we were able to …

Rubbish Habit Or Novel Habitat? Colonization Of Anthropogenic Litter By Stream Invertebrates, Lanie Masset, Jonathan O'Brien

2023 Midwest Ecology & Evolution Conference

We conducted a field experiment to examine the colonization and use of human derived materials by aquatic invertebrates in three western New York streams. The study streams included two soft-bottomed urban streams, Ransom Creek and California Creek, and a hard-bottomed forested stream, Hayes Hollow Creek. In each stream, four different substrate types of similar surface areas: rocks, glass bottles, plastic bottles, and bricks were placed and left in the streams for 6 weeks to promote invertebrate colonization. Baskets filled with both rock and leaf substrates were also placed in the streams for 6 weeks to measure background community composition. The …

Effect Of Functional Trait Variation On Bee Body Temperature, Gabriella A. Muller

2023 Midwest Ecology & Evolution Conference

Bees are both ecologically and economically important pollinators for maintaining agricultural production and the health of many diverse ecosystems. As our world continues to change, bee diversity and population numbers are declining due to increasing temperatures. Functional traits such as body size and coloration can mediate the effects of such harsh thermal conditions on insect performance. Specifically, bee body size can significantly impact thermal parameters, with larger bees gaining and losing heat more slowly than smaller bees due to higher surface area to volume ratios. To obtain a better understanding of these relationships, we studied the variation in body temperature …

Variation In Synthetic Floral Microbiome Composition Is Largely Ignored By Foraging Bumblebees, Nevin P. Cullen

2023 Midwest Ecology & Evolution Conference

Most flowers are occupied by diverse communities of microbes, which can alter floral attractiveness to key pollinators and floral fitness by interacting with floral traits. Different species of flower often acquire unique communities of microbes. Consequently, pollinators are frequently forced to choose between two flowers with distinct floral microbiomes while foraging. However, it is currently unknown whether pollinators have an inherent preference for flowers containing one floral microbiome over another, and whether microbial preference is modified by variation in floral traits. We investigated this knowledge gap using distinct bacteria and yeasts cultured from a nickel-hyperaccumulating plant and its non-accumulating relative. …

Effect Of Variable Canopy Cover On Floral Visitation, Toby Shaya

2023 Midwest Ecology & Evolution Conference

Pollinators provide essential services for ecosystem function. Studies aimed at understanding how availability of floral resources impacts pollinators find that canopy cover impacts bees indirectly by affecting the amount of floral resources in the forest understory and directly by changing the thermal environment in which they can thermoregulate. As ectotherms the maintenance of bee body temperature depends on the distribution of patches of sun and shade throughout a landscape, which can be dependent on canopy cover. The goals of this study were to understand how variable canopy cover drives floral visitation patterns in oak savanna found in the Oak Openings …

The Influence Of Urban Landscape Characteristics And Regional Climate On Bee Diversity, Bridget T. Harter

2023 Midwest Ecology & Evolution Conference

When compared to the lack of resources within homogeneous agricultural lands, cities can serve as a refuge for insect pollinators by providing a variety of floral and nesting resources. Areas of intense urbanization, with a high percentage of impervious surface, reduce the size and quality of pollinator habitat and negatively impact pollinators. The spatial complexity within and between urban landscapes may explain this contradictory view of cities as detrimental and beneficial for insect pollinators. This study examines the influence of (1) the spatial configuration of nearby, herbaceous vegetation and (2) regional climate on bee diversity across cities. Bee abundance and …

Urban Or Rural? Does It Matter To Bumble Bees An Analysis In Kalamazoo, Mi, Elizabeth M. Silber

2023 Midwest Ecology & Evolution Conference

Bumble bee species are declining in vast geographical areas due to many factors, including reductions in suitable nest site locations and floral resources, as land uses shift toward urbanization and agricultural intensification. The potential for urban areas to support bumble bees is understudied. The purpose of this study was to see how environment type impacts bumble bee species richness, abundance, and diversity in both urban and rural environments. Data from private and public spaces was gathered through the usage of citizen science. Observations were solicited under a previously established iNaturalist citizen science project, Southwest Michigan Bee Watch, and past data …

Pre-Dredging Assessment Of Aquatic Macroinvertebrates From Sharon Lake And Creek, Cincinnati, Ohio, Paige M. Loboschefski, Addyson M. Thomas, Emma J. Weiss

2023 Midwest Ecology & Evolution Conference

Dams can serve a multitude of purposes for humans including water storage and flood control. However, dams can also have negative cascading impacts on the surrounding ecosystem. One impact is sedimentation, where reduced water flow can increase the accumulation of benthic sediment. Nutrients can also accumulate leading to eutrophication, affecting organismal and ecosystem health. In order to improve water and ecosystem quality, dredging can occur to remove benthic sediments. The main objective of this study was to characterize the macroinvertebrate community above and below a dam prior to substrate dredging. This study was conducted in Sharon Lake and Creek near …

Defining Best Management Practices For Bee Conservation In Agroforestry Systems, Elijah C. Cardona

2023 Midwest Ecology & Evolution Conference

Wild bees are an indispensable part of neotropical ecosystems because of their role in agriculture and native flora pollination. Despite their importance, wild bees are threatened throughout their distribution by pesticides, habitat loss, land use change and climate change. The objective of this study was to understand how to better manage agroforestry systems for wild bees by asking if wild bee abundance is influenced by the level of shade cover, agroforest type, the number or diversity of floral resources, tree diversity, and the density of trees in an agroforest. A total of 10 agroforests were selected in San Luis de …

Novel Model For Tau Protein Expression And Function: The Honeybee Brain, Abdallah F. Sher

2023 Midwest Ecology & Evolution Conference

Scientists have long attempted to understand the mechanisms by which senescence occurs. Leading theories suggest that aging is an emergent property of natural selection’s prioritization of reproductive ability. Social insects, such as the honeybee (Apis mellifera) challenge this idea, separating colonies into reproductive and non-reproductive castes. Worker honeybees do not reproduce, suggesting that selection prioritizes tasks other than reproduction. Forager honeybees, for example, display improved cognitive and immune function compared to other behavioral phenotypes of bees, likely to support their ability to perform tasks such as navigating complex environments or making context-dependent decisions while foraging. Foragers are normally older honeybees, …

Foraging Ants Dislodged Via Simulated Instantaneous Leaf Movement, Olivia M. Gamsky, Andrew R. Seiler, Alyssa Y. Stark, Stephen P. Yanoviak

2023 Midwest Ecology & Evolution Conference

Cursorial arboreal organisms like ants face numerous challenges to their adhesive systems. Wind, rain, and the activities of vertebrates frequently dislodge foraging ants from tree surfaces. Falling is a significant hazard resulting in substantial displacement, thus ants are predicted to fully engage their tarsal adhesive systems when the likelihood of disturbance is high. Here we used a small catapult to experimentally test the effect of instantaneous red oak (Quercus rubra) leaf movement on the adhesive capabilities of Camponotus pennsylvanicus worker ants. Catapult velocity and displacement mimicked the effects of Eastern Gray Squirrel (Sciurus carolinensis) branch landings. …

Potential Loss Of Pheromone Receptors In The Red Milkweed Beetle, Tetraopes Tetrophthalmus (Coleoptera: Cerambycidae), Mathew A. Price

2023 Midwest Ecology & Evolution Conference

The family of longhorn beetles (Coleoptera: Cerambycidae) are characterized by their elongate antennae, which are well adapted for detecting volatile compounds. This makes them an ideal family to study odorant receptor genes (ORs), which are the rapidly evolving family of proteins that are used by insects to detect odors at the molecular level. Here, we present data on 48 ORs we have sequenced and annotated from an antennal transcriptome of a male red milkweed beetle, Tetraopes tetrophthalmus. In contrast to most cerambycids, T. tetrophthalmus is not known to produce long-range sex pheromones to attract or select mates; correspondingly, …

Polyploidy’S Impact On Plant-Herbivore Dynamics: Evidence From Experiments With Water-Lily Aphids And Greater Duckweed, Hannah R. Assour

2023 Midwest Ecology & Evolution Conference

Polyploidy, or whole-genome duplication, is a widespread mutational process in plants with a myriad of phenotypic and genotypic consequences. In addition to consequences incurred by the plant, polyploidy can potentially cascade to alter species interactions. Yet, knowledge of whether ploidy-induced variation has effects on plant-herbivore dynamics is scarce. Here, we test whether aphid herbivores exhibit preference for a diploid or polyploid host cytotype and whether ploidy impacts plant resistance and tolerance to aphids, measured as aphid and plant performance respectively. To answer these questions, we evaluated aphid choice and compared the effects of the water-lily aphid (Rhopalosiphum nymphaeae) …