Purification And Characterization Of Cytoplasmic Nadp+-
Isocitrate Dehydrogenase, And Amplification Of The Nadp+-Idh Gene From The Wing-Dimorphic Sand Field Cricket, Gryllus Firmus,
2010
University of Nebraska - Lincoln
Purification And Characterization Of Cytoplasmic Nadp+- Isocitrate Dehydrogenase, And Amplification Of The Nadp+-Idh Gene From The Wing-Dimorphic Sand Field Cricket, Gryllus Firmus, Anthony J. Zera, Susan Newman, David Berkheim, Christine Black, Lindsay Klug, Erica Crone
School of Biological Sciences: Faculty Publications
Cytoplasmic NADP+-isocitrate dehydrogenase (NADP+-IDH) has been purified and characterized, and its gene sequenced in many animal, plant, and yeast species. However, much less information is available on this enzyme-gene in insects. As a first step in investigating the biochemical and molecular mechanisms by which NADP+-IDH contributes to adaptations for flight vs. reproduction in insects, the enzyme was purified to homogeneity in the wing-dimorphic cricket, Gryllus firmus, characterized, and its corresponding gene sequenced. Using a combination of polyethylene glycol precipitation, Cibacron-Blue affinity chromatography, and hydrophobic interaction chromatography the enzyme was purified 291-fold (7% yield; specific activity = 15.8 μmol NADPH/min/mg …
Morphology And Systematics Of Braconid Wasps,
2010
University of Kentucky
Morphology And Systematics Of Braconid Wasps, Charles Andrew Boring
University of Kentucky Doctoral Dissertations
The following morphological structures of the ovipositor of Homolobus truncator (Say) (Hymenoptera : Braconidae) are described and hypotheses of their functions are proposed: a series of sharp ridges on the distal surface of the notch helps maintain a grip on the inner surface of the host cuticle; the sperone directs eggs away from the inner surface of the ventral valves; a flap-like structure on each ventral valve covers the portal through which eggs pass; the valvillus maintains position of the egg within the ovipositor and acts against the egg to force it out; ctenidia on the inner surface of the …
Male Remating Success And The Frequency Of Copulatory Plugs In The Green Lynx Spider Peucetia Viridans (Araneae, Oxyopidae),
2010
Loyola Marymount University
Male Remating Success And The Frequency Of Copulatory Plugs In The Green Lynx Spider Peucetia Viridans (Araneae, Oxyopidae), Martina G. Ramirez, Angelik C. Achekian, Catherine R. Coverley, Rachel M. Pierce, Sarah S. Eiman, Melissa M. Wetkowski
Biology Faculty Works
Peucetia viridans males were allowed to mate with three virgin females and most matings resulted in live spiderlings, even when males lacked palpal paracymbial processes. Among females, the presence of copulatory plugs was inconsistent, and when present, their condition was not uniform; broken-off male paracymbia were often found in epigynal orifices. There was no size effect in a male’s ability to completely plug a female’s genital orifices, as well as no significant change in the pattern of plug production over consecutive mating trials. Among mated, field-collected females, the presence of plugs and paracymbia was variable, with females from some sites …
Nestmate Recognition And The Role Of Cuticular Hydrocarbons In The African Termite Raiding Ant Pachycondyla Analis,
2010
International Centre of Insect Physiology and Ecology Nairobi
Nestmate Recognition And The Role Of Cuticular Hydrocarbons In The African Termite Raiding Ant Pachycondyla Analis, Abdullahi A. Yusuf, Christian W.W. Pirk, Robin M. Crewe, Peter G.N. Njagi, Ian Gordon, Baldwyn Torto
All Peer-Reviewed Publications
Cuticular hydrocarbons (CHCs) are used for chemical communication among nestmates in many ant species, and they may play a role in the discrimination of nestmates and non-nestmates. Using the mandible opening response (MOR) bioassay, we tested the response of the African termite raiding ant, Pachycondyla analis, to CHC extracts of nestmates and non-nestmates. The ants were able to distinguish control chemical cues, from nestmate CHCs, and from non-nestmate CHCs, and, based on a CHC recognition threshold, aggression was demonstrated toward non-nestmates. Gas chromatography (GC) and GC-mass spectrometric analyses showed that CHC components of different ant colonies had chain lengths ranging …
The Transition Zone: Impact Of Riverbanks On Emergent Dragonfly Nymphs. Implications For Riverbank Restoration And Management,
2010
Antioch University of New England
The Transition Zone: Impact Of Riverbanks On Emergent Dragonfly Nymphs. Implications For Riverbank Restoration And Management, Kirsten Hope Martin
Antioch University Dissertations & Theses
The use of riprap in the restoration and stabilization of riverine landscapes is an issue of concern for many ecologists. While current methods of bank stabilization, especially those involving the placement of rocks (riprap) along the waterline, are effective in controlling erosion their presence changes habitat components (slope, substrate composition, near-shore river velocity) at the river-land interface. The additional impacts of river current, water temperature, soil composition, slope, and water level fluctuation, may further imperil emerging nymphs. The purpose of this research is to document the effects of riprap, location (upriver or downriver of hydroelectric intake/outtake facilities), water level fluctuation, …
Host Recognition And Acceptance Behaviour In Cotesia Sesamiae And C. Flavipes (Hymenoptera: Braconidae), Parasitoids Of Gramineous Stemborers In Africa,
2010
North-West University
Host Recognition And Acceptance Behaviour In Cotesia Sesamiae And C. Flavipes (Hymenoptera: Braconidae), Parasitoids Of Gramineous Stemborers In Africa, Meshack Obonyo, Fritz Schulthess, Bruno Le Ru, Johnnie Van Den Berg, Paul André Calatayud
All Peer-Reviewed Publications
The host recognition and acceptance behaviour of two braconid larval parasitoids (Cotesia sesamiae and C. flavipes) were studied using natural stemborer hosts (i.e., the noctuid Busseola fusca for C. sesamiae, and the crambid Chilo partellus for C. flavipes) and a non-host (the pyralid Eldana saccharina). A single larva was introduced into an arena together with a female parasitoid and the behaviour of the wasp recorded until it either stung the larva or for a maximum of 5 min if it did not sting the larva. There was a clear hierarchy of behavioural steps, which was similar for both parasitoid species. …
Cockroaches,
2010
Utah State University
Cockroaches, Ryan S. Davis
All Current Publications
Cockroaches are one of the most difficult structural pests to eradicate because of their ability to hide, rapid reproduction, and resistance to and avoidance of many commonly used insecticide sprays and baits. Using an integrated pest management (IPM) program can greatly increase the possibility of successful control. There are 4 cockroach species that commonly infest structures in Utah, each with a specific biology; identify invading cockroaches before you develop an IPM plan. Proper identification of any pest will allow you to understand its biology, and use it against itself!
Importance Of Contact Chemical Cues In Host Recognition And Acceptance By The Braconid Larval Endoparasitoids Cotesia Sesamiae And Cotesia Flavipes,
2010
North-West University
Importance Of Contact Chemical Cues In Host Recognition And Acceptance By The Braconid Larval Endoparasitoids Cotesia Sesamiae And Cotesia Flavipes, Meshack Obonyo, Fritz Schulthess, Bruno Le Ru, Johnnie Van Den Berg, Jean François Silvain, Paul André Calatayud
All Peer-Reviewed Publications
The ability of the congeneric braconid parasitoids Cotesia sesamiae (Cameron) and Cotesia flavipes Cameron to discriminate between stemborer larval cues upon contact was studied using their natural hosts, namely the noctuid Busseola fusca (Fuller) and the crambid Chilo partellus (Swinhoe), respectively, and the pyralid non-host Eldana saccharina (Walker). When the natural host larvae were washed in distilled water, parasitoid behavior was similar to that displayed when in contact with E. saccharina, characterized by the absence of ovipositor insertion. When washed host or non-host larvae were bathed with water extracts of their natural host, the parasitoids showed a significant increase in …
Expression Of Metallothionein And Α-Tubulin In Heavy Metal-Tolerant Anopheles Gambiae Sensu Stricto (Diptera: Culicidae),
2010
Egerton University
Expression Of Metallothionein And Α-Tubulin In Heavy Metal-Tolerant Anopheles Gambiae Sensu Stricto (Diptera: Culicidae), Paul O. Mireji, Joseph Keating, Ahmed Hassanali, Daniel E. Impoinvil, Charles M. Mbogo, Martha N. Muturi, Hudson Nyambaka, Eucharia U. Kenya, John I. Githure, John C. Beier
All Peer-Reviewed Publications
Anopheles mosquitoes have been shown to adapt to heavy metals in their natural habitats. In this study we explored the possibility of using Anopheles gambiae sensu stricto as bio-reporters for environmental heavy metal pollution through expressions of their metal-responsive metallothionein and α-tubulin genes. The study was undertaken with third instar larvae after selection by cadmium, copper, or lead at LC30 through five successive generations. Expression levels were determined in the 5th generation by semi-quantitative RT-PCR on the experimental and control populations. The data were analyzed using one-way ANOVA. The highest metallothionein (F3, 11=4.574, P=0.038) and α-tubulin (F3, 11=12.961, P=0.002) responses …
Environmental Correlates Of Abundances Of Mosquito Species And Stages In Discarded Vehicle Tires,
2010
University of Southern Mississippi
Environmental Correlates Of Abundances Of Mosquito Species And Stages In Discarded Vehicle Tires, Donald A. Yee, Jamie M. Kneitel, Steven A. Juliano
Faculty Publications
Discarded vehicle tires are a common habitat for container mosquito larvae, although the environmental factors that may control their presence or abundance within a tire are largely unknown. We sampled discarded vehicle tires in six sites located within four counties of central Illinois during the spring and summer of 2006 to determine associations between a suite of environmental factors and community composition of container mosquitoes. Our goal was to find patterns of association between environmental factors and abundances of early and late instars. We hypothesized that environmental factors correlated with early instars would be indicative of oviposition cues, whereas environmental …
