Entomology Commons^™

Empirical And Conceptual Approaches To Life-History Trade-Offs: The Size And Number Of Offspring In Broods Of A Parasitoid Wasp, Yoriko Saeki

Theses and Dissertations--Biology

Trade-offs in resource allocation underlie key life history traits of organisms. My dissertation focuses on the size-number trade-off in clonal broods of offspring using the polyembryonic wasp, Copidosoma bakeri parasitizing immature stages of the moth Agrotis ipsilon. I aim to characterize responses of wasp brood size and individual body mass by manipulating the environments in order to understand the allocation pattern in the size number trade-off. In reviewing the functional forms of trade-off relationships in relation to resource constraints, I distinguish among three main trade-off types based on graphical representations of the relationship between the trade-off variables: linear, convex …

Nepetalactones From Essential Oil Of Nepeta Cataria Represent A Stable Fly Feeding And Oviposition Repellent, J. J. Zhu, Dennis R. Berkebile, C. E. Dunlap, A. Zhang, D. Boxler, K. Tangtrakulwanich, R. Behle, Frederick P. Baxendale, Gary J. Brewer

Department of Entomology: Faculty Publications

The stable fly, Stomoxys calcitrans (L.) (Diptera: Muscidae), is one of the most serious pests to livestock. It feeds mainly on cattle and causes significant economic losses in the cattle industry. Standard stable fly control involving insecticides and sanitation is usually costly and often has limited effectiveness. As we continue to evaluate and develop safer fly control strategies, the present study reports on the effectiveness of catnip (Nepeta cataria L.) oil and its constituent compounds, nepetalactones, as stable fly repellents. The essential oil of catnip reduced the feeding of stable flies by >96% in an in vitro bioassay system, …

Ipm Team Representatives, Clyde L. Ogg, Erin Bauer

Department of Entomology: Faculty Publications

Contents

Acknowledgments...................... iv
Preface...................... v
Introduction...................... 1
Chapter 1 – What is Integrated Pest Management...................... 3
Chapter 2 – Monitoring and Inspecting...................... 15
Chapter 3 – Injury and Action Levels...................... 37
Chapter 4 – Treatment Strategies...................... 41
Chapter 5 – How to Develop an IPM Program...................... 49
Chapter 6 – IPM for Ants...................... 59
Chapter 7 – IPM for Cockroaches...................... 69
Chapter 8 – IPM for Bed Bugs...................... 81
Chapter 9 – IPM for Fabric and Pantry Pests...................... 87
Chapter 10 – IPM for Fleas...................... 95
Chapter 11 – IPM for Flies...................... 101
Chapter 12 – IPM for Head Lice...................... …

Using Web-Based Key Character And Classification Instruction For Teaching Undergraduate Students Insect Identification, Douglas A. Golick, Tiffany M. Heng-Moss, Allen L. Steckelberg, David W. Brooks, Leon G. Higley, David Fowler

Department of Entomology: Faculty Publications

The purpose of the study was to determine whether undergraduate students receiving web-based instruction based on traditional, key character, or classification instruction differed in their performance of insect identification tasks. All groups showed a significant improvement in insect identifications on pre- and post-two-dimensional picture specimen quizzes. The study also determined student performance on insect identification tasks was not as good as for family-level identification as compared to broader insect orders and arthropod classification identification tasks. Finally, students erred significantly more by misidentification than misspelling specimen names on prepared specimen quizzes. Results of this study support that short web-based insect identification …

Inter- And Intraspecific Identification Of The Screwworm, Cochliomyia Hominivorax, Using Random Amplified Polymorphic Dna-Polymerase Chain Reaction, Steven R. Skoda, James L. Figarola, Saowaluck Pornkulwat, John E. Foster

Department of Entomology: Faculty Publications

The screwworm, Cochliomyia hominivorax (Coquerel) (Diptera: Calliphoridae), is one of the most devastating arthropod pests of livestock in the Western Hemisphere. Early instars are very difficult to distinguish morphologically from several closely related blow fly species. Random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) markers were developed for identifying C. hominivorax from other wound inhabiting species. Forty decameric primers were screened; nine showed clear reproducible RAPD profiles suitable for distinguishing all life stages of C. hominivorax from 7 other species, including C. macellaria (Fabricius). The results from RAPD-PCR with field-collected samples of unknown first instars agreed with morphological identification that the …

Larval Survival And Plant Injury Of Cry1ab-Susceptible, -Resistant, And -Heterozygous Genotypes Of The Sugarcane Borer On Transgenic Corn Containing Single Or Pyramided Bt Genes, David S. Wangila, B. Rogers Leonard, Yaoyu Bai, Graham P. Head, Fangneng Huang

Department of Entomology: Faculty Publications

Transgenic corn (Zea mays L.) products expressing multiple Bt proteins targeting a same group of insect pests have become commercially available in the U.S. The first commercialized pyramided Bt corn technologies for managing lepidopteran pests include Genuity® VT Triple Pro™ and Genuity® SmartStax™. In this study, larval survival and plant injury of Cry1Ab-susceptible (Cry1Ab-SS), -resistant (Cry1Ab-RR), and -heterozygous (Cry1Ab-RS) genotypes of the sugarcane borer, Diatraea saccharalis F., on five commercial corn hybrids were evaluated in a leaf tissue bioassay and two greenhouse trials during 2010–2011. The five hybrids included two non-Bt corn and three Bt corn hybrids representing three …

Mortality Impact Of Mon863 Transgenic Maize Roots On Western Corn Rootworm Larvae In The Field, T. L. Clark, D. L. Frank, B. W. French, Lance J. Meinke, D. Moellenbeck, T. T. Vaughn, B. E. Hibbard

Department of Entomology: Faculty Publications

Mortality of western corn rootworm (Diabrotica virgifera virgifera LeConte) due to feeding on MON863 transgenic maize (Zea mays L.) expressing the Cry3Bb1 protein was evaluated at three Missouri sites in both 2003 and 2004 and at one site each in South Dakota, Nebraska, and Iowa in 2004. To do this, survivorship relative to survivorship on isoline maize (i.e., the same genetic background, but without Cry3Bb1) was evaluated. Comparisons were made using low (1650–2500 eggs/m) and high (3300–3500 eggs/m) western corn rootworm egg densities. Significantly fewer beetles were recovered from MON863 than from isoline maize. Emergence from MON863 as …

Using Sex Pheromone Traps In The Decision-Making Process For Pesticide Application Against Fall Armyworm (Spodoptera Frugiperda [Smith] [Lepidoptera: Noctuidae]) Larvae In Maize, Ivan Cruz, Maria De Lourdes Corrêa Figueiredo, Rafael Braga Da Silva, Ivana Fernandes Da Silva, Cristiane De Souza Paula, John E. Foster

Department of Entomology: Faculty Publications

The fall armyworm, Spodoptera frugiperda (Smith) (Lepidoptera: Noctuidae), is a major pest of maize and frequently demands control measures. The timing of insecticide application is a key factor in determining its efficiency, so an experiment was designed to investigate this. Application of insecticide was based on three criteria: (i) the number of trap-caught moths in a Delta-type trap with a commercial sex pheromone lure placed in the center of the target area, soon after plant emergence; (ii) the percentage of plants exhibiting pinhole-type damage (10% or 20%), and (iii) the percentage of plants exhibiting shot hole–type damage (10% or 20%) …

Comparative Toxicity Of Acaricides To Honey Bee (Hymenoptera: Apidae) Workers And Queens, Lizette Dahlgren, Reed M. Johnson, Blair D. Siegfried, Marion D. Ellis

Department of Entomology: Faculty Publications

Acaricides are used to treat honey bee (Apis mellifera L.) colonies to control the varroa mite (Varroa destructor Anderson & Trueman), a worldwide threat to honey bee health. Although acaricides control a serious honey bee parasite and mitigate bee loss, they may cause harm to bees as well. We topically applied five acaricides, each with a different mode of action, to young adult queen and worker bees to generate dose-response curves and LD₅₀. Twenty-four hours after treatment, queens were found to be three times more tolerant of tau-fluvalinate and six times more tolerant of thymol than …

Human Risk Of Infection With Borrelia Burgdorferi, The Lyme Disease Agent, In Eastern United States, Maria A. Diuk-Wasser, Anne Gatewood Hoen, Paul Cislo, Robert Brinkerhoff, Sarah A. Hamer, Michelle Rowland, Roberto Cortinas, Gwenaël Vourc’H, Forrest S. Melton, Graham J. Hickling, Jean I. Tsao, Jonas Bunikis, Alan G. Barbour, Uriel Kitron, Joseph Piesman, Durland Fish

Department of Entomology: Faculty Publications

The geographic pattern of human risk for infection with Borrelia burgdorferi sensu stricto, the tick-borne pathogen that causes Lyme disease, was mapped for the eastern United States. The map is based on standardized field sampling in 304 sites of the density of Ixodes scapularis host-seeking nymphs infected with B. burgdorferi, which is closely associated with human infection risk. Risk factors for the presence and density of infected nymphs were used to model a continuous 8 km × 8 km resolution predictive surface of human risk, including confidence intervals for each pixel. Discontinuous Lyme disease risk foci were identified in …

Morphological Characterization And Molecular Mediated Genetic Variation Of Thief Ants (Hymenoptera: Formicidae), Ralph B. Narain, Shripat T. Kamble, Thomas O. Powers

Department of Entomology: Faculty Publications

The morphological characterization and molecular genetic variation were determined in populations of thief ants, Solenopsis molesta (Say). The genetic variations were elucidated using mitochondrial deoxyribonucleic acid (mDNA) sequences of cytochrome oxidase I. DNA from thief ants was extracted with Qiagen’s Gentra PUREGENE DNA Isolation Kit using their solid tissue protocol. Polymerase chain reactions (PCR) were run on the extracted DNA using primers Lep-F1 (forward) and Lep-R1 (reverse). The DNA products were concentrated and purified by Microcon Centrifugal Filter Unit YM-100. Purified DNA samples were sequenced at the University of Arkansas Medical Sciences (UAMS) DNA Sequencing Core Facility. The sequences were …

Inbreeding-Stress Interactions: Evolutionary And Conservation Consequences, David H. Reed, Charles W. Fox, Laramy S. Enders, Torsten N. Kristensen

Department of Entomology: Faculty Publications

The effect of environmental stress on the magnitude of inbreeding depression has a long history of intensive study. Inbreeding-stress interactions are of great importance to the viability of populations of conservation concern and have numerous evolutionary ramifications. However, such interactions are controversial. Several meta-analyses over the last decade, combined with omic studies, have provided considerable insight into the generality of inbreeding-stress interactions, its physiological basis, and have provided the foundation for future studies. In this review, we examine the genetic and physiological mechanisms proposed to explain why inbreeding-stress interactions occur. We specifically examine whether the increase in inbreeding depression with …

Susceptibility Of Cry1ab Maize-Resistant And -Susceptible Strains Of Sugarcane Borer (Lepidoptera: Crambidae) To Four Individual Cry Proteins, Liping Zhang, Fangneng Huang, B. Rogers Leonard, Mao Chen, Thomas Clark, Yu Cheng Zhu, David S. Wangila, Fei Yang, Ying Niu

Department of Entomology: Faculty Publications

Sugarcane borer, Diatraea saccharalis (F.), is a major target of Bt maize in South America and many areas of the US mid-south region. Six laboratory strains of D. saccharalis were established from six single-pair F2 families possessing major resistance alleles to Cry1Ab maize hybrids. Susceptibility of the six strains was evaluated on diet treated with each of four purified trypsin-activated Bt proteins, Cry1Ab, Cry1Aa, Cry1Ac, and Cry1F. Bt susceptibility of the six strains was compared with that of known Cry1Ab-susceptible and -resistant strains of D. saccharalis. At least two of the six strains demonstrated a similar level (>526-fold) …

Using Video-Tracking To Assess Sublethal Effects Of Pesticides On Honey Bees (Apis Mellifera L.), Bethany S. Teeters, Reed M. Johnson, Marion D. Ellis, Blair D. Siegfried

Department of Entomology: Faculty Publications

Concern about the role of pesticides in honey bee decline has highlighted the need to examine the effects of sublethal exposure on bee behaviors. The video-tracking system EthoVisionXT (Noldus Information Technologies) was used to measure the effects of sublethal exposure to tau-fluvalinate and imidacloprid on honey bee locomotion, interactions, and time spent near a food source over a 24-h observation period. Bees were either treated topically with 0.3, 1.5, and 3 μg tau-fluvalinate or exposed to 0.05, 0.5, 5.0, 50, and 500 ppb imidacloprid in a sugar agar cube. Tau-fluvalinate caused a significant reduction in distance moved at all dose …

A Comparison Of Dung Beetle (Coleoptera: Scarabaeidae) Attraction To Native And Exotic Mammal Dung, Sean D. Whipple, W. Wyatt Hoback

Department of Entomology: Faculty Publications

Although the preference of dung beetles (Coleoptera: Scarabaeidae) for specific types and conditions of dung has been given substantial attention, little has been done to investigate the potential effects of exotic mammal introduction for game farms or rewilding projects. We used pitfall traps baited with various native and exotic herbivore, carnivore, and omnivore dung to evaluate dung beetle preference in the Great Plains of North America. Additionally, we analyzed the nutrient quality of each dung type. In total, 9,089 dung beetles from 15 species were captured in 2 yr of sampling. We found significant differences (P < 0.05) in mean dung beetle capture among omnivore, herbivore, and carnivore dung as well as differences in individual species preference for dung type. Omnivore dung was the most attractive with chimpanzee and human dung having the highest mean capture (291.1 ± 27.6 and 287.5 ± 28.5 respectively). Carrion also was highly attractive with a mean of 231.9 ± 20.6 beetles per trap (N = 8). …

Genetic Variability Of Digitonthophagus Gazella (F.) (Coleoptera: Scarabaeidae) From Vieques, Puerto Rico And South Africa, Sean D. Whipple, Erica Lindroth, W. Wyatt Hoback, Marc C. Albrecht, John E. Foster

Department of Entomology: Faculty Publications

Digitonthophagus gazella (F.) has been widely introduced to the New World and both natural dispersal and intentional releases continue. In this study, we compare a population of D. gazella from South Africa and from the island of Vieques, Puerto Rico, using amplified fragment length polymorphism (AFLP) analysis. Genetic diversity was found to be high in both South Africa (H = 0.3623) and Vieques (H = 0.3846), providing no evidence of inbreeding depression on Vieques. Analysis of molecular variance (AMOVA) revealed that 69% of genetic diversity is within the populations and 31% of genetic diversity is between the populations, indicating that …

Population Genetics Of The Western Bean Cutworm (Lepidoptera: Noctuidae) Across The United States, Erica Lindroth, T. E. Hunt, S. R. Skoda, M. D. Culy, D. Lee, John E. Foster

Department of Entomology: Faculty Publications

The western bean cutworm, Striacosta albicosta (Smith), is a secondary pest of maize (Zea mays L.) and dry beans (Phaseolus vulgaris L.) in the western United States. Recently, this insect has undergone a major territory expansion into the eastern United States and has become a pest throughout much of the Corn Belt. This study was instigated to examine the population genetics of this pest to facilitate control and resistance management, as well as to shed light on the current habitat expansion. S. albicosta individuals were collected from 24 different locations across the traditional and expanded range and amplifed …

Pheromone–Mediated Mating Disruption In The Millet Stem Borer, Coniesta Ignefusalis (Lepidoptera: Pyralidae), Ousmane Youm, Yacouba Maliki, David R. Hall, Dudley I. Farman, John E. Foster

Department of Entomology: Faculty Publications

The millet stem borer, Coniesta ignefusalis Hampson (Lepidoptera: Pyralidae), is a major pest of pearl millet in the Sahelian region of Africa. The female sex pheromone has been identified and synthesized, and previous research had shown that the synthetic pheromone could cause high levels of reproductive communication disruption in small plots when released at rates of 640 mg/ha/day, using PVC resin formulation renewed every seven days to maintain efficiency. In the present research, in experiments in farmers’ fields in Niger, 86.8% (SE = 2.6%) communication disruption was achieved when polyethylene vials loaded with 0.5 mg pheromone at 400 dispensers/ha were …

Increase In Susceptibility To Insecticides With Aging Of Wild Anopheles Gambiae Mosquitoes From Côte D’Ivoire, Mouhamadou S. Chouaibou, Joseph Chabi, Georgina Bingham, Tessa B. Knox, Louis N'Dri, Nestor B. Kesse, Bassirou Bonfoh, Helen V Pates Jamet

Department of Entomology: Faculty Publications

Background: Appropriate monitoring of vector insecticide susceptibility is required to provide the rationale for optimal insecticide selection in vector control programs.

Methods: In order to assess the influence of mosquito age on susceptibility to various insecticides, field-collected larvae of An. gambiae s.l. from Tiassalé were reared to adults. Females aged 1, 2, 3, 5 and 10 days were exposed to 5 insecticides (deltamethrin, permethrin, DDT, malathion and propoxur) using WHO susceptibility test kits. Outcome measures included the LT50 (exposure time required to achieve 50% knockdown), the RR (resistance ratio, i.e. a calculation of how much more resistant the wild population …

Larval Performance And Kill Rate Of Convergent Ladybird Beetles, Hippodamia Convergens, On Black Bean Aphids, Aphis Fabae, And Pea Aphids, Acyrthosiphon Pisum, Travis M. Hinkelman, Brigitte Tenhumberg

School of Biological Sciences: Faculty Publications

Generalist predator guilds play a prominent role in structuring insect communities and can contribute to limiting population sizes of insect pest species. A consequence of dietary breadth, particularly in predatory insects, is the inclusion of low-quality, or even toxic, prey items in the predator’s diet. Consumption of low-quality prey items reduces growth, development, and survival of predator larvae, thereby reducing the population sizes of generalist predators. The objective of this paper was to examine the effect of a suspected low-quality aphid species, Aphis fabae (Scopoli) (Hemiptera: Aphididae), on the larval performance of an abundant North American predator, Hippodamia convergens (Guérin-Méneville) …