Entomology Commons^™

Patterns Of Differential Gene Expression In Adult Rotation-Resistant And Wild-Type Western Corn Rootworm Digestive Tracts, Chia-Ching Chu, Jorge A. Zavala, Joseph L. Spencer, Matías J. Curzi, Christopher J. Fields, Jenny Drnevich, Blair D. Siegfried, Manfredo J. Seufferheld

Department of Entomology: Faculty Publications

The western corn rootworm (WCR, Diabrotica virgifera virgifera LeConte) is an important pest of corn. Annual crop rotation between corn and soybean disrupts the corn-dependent WCR life cycle and is widely adopted to manage this pest. This strategy selected for rotation-resistant (RR) WCR with reduced ovipositional fidelity to corn. Previous studies revealed that RR-WCR adults exhibit greater tolerance of soybean diets, different gut physiology, and host–microbe interactions compared to rotation-susceptible wild types (WT). To identify the genetic mechanisms underlying these phenotypic changes, a de novo assembly of the WCR adult gut transcriptome was constructed and used for RNA-sequencing analyses of …

Parental Rna Interference Of Genes Involved In Embryonic Development Of The Western Corn Rootworm, Diabrotica Virgifera Virgifera Leconte, Chitvan Khajuria, Ana Maria Vélez, Murugesan Rangasamy, Haichuan Wang, Elane Fishilevich, Meghan L.F. Frey, Newton Portilho Carneiro, Premchand Gandra, Kenneth E. Narva, B. D. Siegfried

Department of Entomology: Faculty Publications

RNA interference (RNAi) is being developed as a potential tool for insect pest management and one of the most likely target pest species for transgenic plants that express double stranded RNA (dsRNA) is the western corn rootworm. Thus far, most genes proposed as targets for RNAi in rootworm cause lethality in the larval stage. In this study, we describe RNAi-mediated knockdown of two developmental genes, hunchback (hb) and brahma (brm), in the western corn rootworm delivered via dsRNA fed to adult females. dsRNA feeding caused a significant decrease in hb and brm transcripts in the adult …

Selection Of Reference Genes For Rt-Qpcr Analysis In The Monarch Butterfly, Danaus Plexippus (L.), A Migrating Bio-Indicator, Huipeng Pan, Xiaowei Yang, Keith Bidne, Richard L. Hellmich, Blair D. Siegfried, Xuguo Zhou

Department of Entomology: Faculty Publications

Reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) is a powerful technique to quantify gene expression. To facilitate gene expression study and obtain accurate results, normalization relative to stably expressed reference genes is crucial. The monarch butterfly, Danaus plexippus (L.), is one of the most recognized insect species for its spectacular annual migration across North America. Besides its great voyages, D. plexippus has drawn attention to its role as a bio-indicator, ranging from genetically modified organisms (GMOs) to natural ecosystems. In this study, nine reference genes from D. plexippus genome were selected as the candidate reference genes. The expression profiles of these …

Early Detection And Mitigation Of Resistance To Bt Maize By Western Corn Rootworm (Coleoptera: Chrysomelidae), David A. Andow, Steven G. Pueppke, Arthur W. Schaafsma, Aaron J. Gassmann, Thomas W. Sappington, Lance Meinke, Paul D. Mitchell, Terrance M. Hurley, Richard L. Hellmich, R. Pat Porter

Department of Entomology: Faculty Publications

Transgenic Bt maize that produces less than a high-dose has been widely adopted and presents considerable insect resistance management (IRM) challenges.Western corn rootworm, Diabrotica virgifera virgifera LeConte, has rapidly evolved resistance to Bt maize in the field, leading to local loss of efficacy for some corn rootworm Bt maize events. Documenting and responding to this resistance has been complicated by a lack of rapid diagnostic bioassays and by regulatory triggers that hinder timely and effective management responses. These failures are of great concern to the scientific and agricultural community. Specific challenges posed by western corn rootworm resistance to Bt maize, …

A Monographic Revision Of The Genus Hoplopyga Thomson, 1880 (Coleoptera: Scarabaeidae: Cetoniinae: Gymnetini), Jennifer M. Shaughney, Brett C. Ratcliffe

Department of Entomology: Faculty Publications

A comprehensive revision of the genus Hoplopyga Thomson, 1880 is presented. Eighteen species are redescribed, and two new species are described. A key for identification, distribution maps, and images of the 20 species are provided. Hoplopyga ruteri Antoine is removed from the genus and placed in junior synonymy with Macrocranius similis Schürhoff. Lectotypes are designated for Hoplopyga brasiliensis (Gory and Percheron), Hoplopyga corumbana Schürhoff, and Hoplopyga multipunctata (Gory and Percheron). The following new synonymies are established: Hoplopyga lucidiventris (Thomson) is Hoplopyga foeda (Schaum); Hoplopyga spurca (Janson) is Hoplopyga liturata (Olivier); Hoplopyga monacha (Gory and Percheron) and Hoplopyga rubida (Gory and …

Short-Horned Grasshopper Subfamilies Feed At Different Rates On Big Bluestem And Switchgrass Cultivars, Muhammad Ullah, Fatima Mustafa, William W. Hoback, Mathew L. Brust, Kerri M. Farnsworth-Hoback, John E. Foster

Department of Entomology: Faculty Publications

Grasshopper species belonging to subfamilies Melanoplinae, Gomphocerinae and Oedipodinae were tested for their feeding rate on three types of grass. All grasshopper species were offered Shawnee and Kanlow cultivars of switchgrass, Panicum virgatum L. and big bluestem, Andropogon gerardii Vitman. The grasshoppers, Melanoplus femurrubrum and Melanoplus differentialis were also tested for their feeding on turgid or wilted leaves of the Shawnee cultivar of switchgrass. We found that M. differentialis consumed more switchgrass compared to big bluestem while M. femurrubrum and Arphia xanthoptera consumed the most Shawnee switchgrass. The M. differentialis consumed more turgid grass compared to wilted switchgrass. The feeding …

Genome-Wide Transcript Profiling Reveals Novel Breast Cancer-Associated Intronic Sense Rnas, Sang Woo Kim, Elane Fishilevich, Gustavo Arango-Argoty, Yuefeng Lin, Guodong Liu, Zhihua Li, A. Paula Monaghan, Mark Nichols, Bino John

Department of Entomology: Faculty Publications

Non-coding RNAs (ncRNAs) play major roles in development and cancer progression. To identify novel ncRNAs that may identify key pathways in breast cancer development, we performed high-throughput transcript profiling of tumor and normal matched-pair tissue samples. Initial transcriptome profiling using high-density genome-wide tiling arrays revealed changes in over 200 novel candidate genomic regions that map to intronic regions. Sixteen genomic loci were identified that map to the long introns of five key protein-coding genes, CRIM1, EPAS1, ZEB2, RBMS1, and RFX2. Consistent with the known role of the tumor suppressor ZEB2 in the cancer-associated epithelial to mesenchymal transition (EMT), in situ …

Behavioural Responses Of Stable Flies To Cattle Manure Slurry Associated Odourants, K Tangtrakulwanich, T A. Albuquerque, G. J. Brewer, F. P. Baxendale, L Zurek, D N. Miller, D. B. Taylor, K A. Friesen, J. J. Zhu

Department of Entomology: Faculty Publications

Stable flies (Stomoxys calcitrans [Diptera: Muscidae] L.) are blood-feeding synanthropic pests, which cause significant economic losses in livestock. Stable fly antennae contain olfactory sensilla responsive to host and host environment-associated odours. Field observation indicated that the abundance of stable flies increased significantly in grasslands or crop fields when cattle manure slurry was applied. Major volatile compounds emanating from manure slurry were collected and identified. Behavioural responses of stable flies to those compounds were investigated in laboratory bioassays and field-trapping studies. Results from olfactometer assays revealed that phenol, p-cresol and m-cresol were attractive to adult stable flies. When …

Comparisons Of Antifeedancy And Spatialrepellency Of Three Natural Product Repellents Against Horn Flies, Haematobia Irritans (Diptera:Muscidae), Junwei J. Zhu, Gary J. Brewer, David J. Boxler, Kristina Friesen, David B. Taylor

Department of Entomology: Faculty Publications

BACKGROUND: Horn flies are among the most important biting fly pests of cattle in the United States. Horn fly management is largely dependent upon pesticides, which ultimately leads to the rapid development of insecticide resistance. Alternative control strategies, including repellents, have shown promising results in reducing fly biting. In the present study, we examined the efficacy and longevity of recently identified natural product repellents against horn flies.

RESULTS: Catnip oil, geraniol and C8910 acids reduced horn fly feeding in a laboratory bioassay and also exhibited spatial repellency in the olfactometer. Residual activity was observed for up to 3 days in …

A Protocol To Preserve The Integrity Of Stable Fly (Diptera: Muscidae) Dna For Long Distance Shipment, K. M. Kneeland, Steven R. Skoda, Elizabeth A. Claassen, John E. Foster

Department of Entomology: Faculty Publications

Population genetic studies on a global scale may be hampered by the ability to acquire quality samples from distant countries. Preservation methods must be adequate to prevent samples from decay during shipping, so an adequate quantity of quality DNA can be extracted for analysis, and materials used must not be prohibited by the pertinent Postal Service. We tested a shipping protocol for stable flies which preserves the samples and adheres to Postal regulations. Samples were soaked in 95% ethanol for 6, 12, 24 and 48 hours; the ethanol was then poured off. Samples remained 'dry' for 2, 4, 6 and …

Herbivore Response To Soybean Under Differing Induction Methods, John L. Dryburgh

LSU Master's Theses

Plants are attacked by a variety of herbivore feeding guilds and respond with specific responses to specific attacks, which may be localized or systemic. How a plant defends against one feeding guild may alter the plant’s response against a different feeding guild. A better understanding of these interactions will allow for the development of refined pest management programs. One situation in which this may occur is in interactions between chewing and piercing/sucking herbivores, such as aphids. Aphids are important crop pests primarily due to their ability to transmit viruses, the efficacy of which can be affected by plant defenses. To …

Effects Of Detrital Subsidy On Arthropod Communities In Louisiana Rice Fields And Predation On Rice Water Weevil (Lissorhoptrus Oryzophilus), Nathan Mercer

LSU Master's Theses

The rice water weevil (RWW), Lissorhoptrus oryzophilus (Kuschel), is the most important insect pest of rice in the United States. Integrated pest management strategies for RWW in Louisiana consist of cultural controls, resistant cultivars and chemical insecticides. The fourth component of IPM, biological control, is largely absent from the literature for RWW, making exploration of biological control a logical next step in developing a full set of IPM strategies. The three main types of biological control are augmentation, classical and conservation. With little known about RWW predators, conservation biological control makes the most sense as local natural enemy abundance is …

Effectiveness Of An Artisanal Mcphail Trap For Mass Trapping Tephritid Fruit Flies In Haiti, Fractyl Mertilus

LSU Master's Theses

Three field experiments were conducted in a mango orchard in Croix-des-bouquets (Haïti) to develop an effective artisanal McPhail trap, less expensive than the commercial traps, for mass trapping Anastrepha obliqua (Macquart) and Anastrepha suspensa (Loew). A field trial conducted to evaluate the effectiveness of two newly made artisanal trap models (AT1 and AT2) with the commercial McPhail trap (MP) demonstrated that the artisanal traps yielded similar results in the average number of fruit flies caught (8.9±2.6, 13±2.9, and 16±4.1 respectively). Moreover, the cost-efficacy ratio was a lot higher in the artisanal trap models (AT1: 0.42 $ per flies caught, AT2: …

Dopamine Receptor Antagonists As New Mode-Of-Action Insecticide Leads For Control Of Aedes And Culex Mosquito Vectors, Andrew B. Nuss, Karin F K Ejendal, Trevor B. Doyle, Jason M. Meyer, Val J. Watts, Catherine A. Hill

Department of Entomology Faculty Publications

New mode-of-action insecticides are sought to provide continued control of pesticide resistant arthropod vectors of neglected tropical diseases (NTDs). We previously identified antagonists of the AaDOP2 D1-like dopamine receptor (DAR) from the yellow fever mosquito, Aedes aegypti, with toxicity to Ae. aegypti larvae as leads for novel insecticides. To extend DAR-based insecticide discovery, we evaluated the molecular and pharmacological characteristics of an orthologous DAR target, CqDOP2, from Culex quinquefasciatus, the vector of lymphatic filariasis and West Nile virus. CqDOP2 has 94.7% amino acid identity to AaDOP2 and 28.3% identity to the human D1-like DAR, hD1. CqDOP2 and AaDOP2 exhibited similar …

Metatranscriptomic Profiles Of Eastern Subterranean Termites, Reticulitermes Flavipes (Kollar) Fed On Second Generation Feedstocks, Swapna Priya Rajarapu, Jacob T. Shreve, Ketaki P. Bhide, Jyothi Thimmapuram, Michael E. Scharf

Department of Entomology Faculty Publications

Background: Second generation lignocellulosic feedstocks are being considered as an alternative to first generation biofuels that are derived from grain starches and sugars. However, the current pre-treatment methods for second generation biofuel production are inefficient and expensive due to the recalcitrant nature of lignocellulose. In this study, we used the lower termite Reticulitermes flavipes (Kollar), as a model to identify potential pretreatment genes/enzymes specifically adapted for use against agricultural feedstocks. Results: Metatranscriptomic profiling was performed on worker termite guts after feeding on corn stover (CS), soybean residue (SR), or 98% pure cellulose (paper) to identify (i) microbial community, (ii) pathway …

Assessing Chemical Control Of Earthworms At Airports, Thomas W. Seamans, Bradley F. Blackwell, Glen E. Bernhardt, Daniel A. Potter

United States Department of Agriculture Wildlife Services: Staff Publications

Earthworms originating from Europe (e.g., Lumbricus spp., Aporrectodea spp.), which are common in the United States and southern Canada can create hazardous conditions at airports by attracting birds that pose a threat to aircraft. These nonnative earthworms are also considered pests on golf courses and sports fields, as well as having detrimental effects on temperate boreal forests. No toxicants or repellents are currently registered for earthworm control in the United States. Our purpose was to identify products that could be used to repel or suppress nonnative earthworms on airports or other managed sites where they pose a hazard or nuisance. …

Identification, Description, And Activity Of Proteins In The Tergal Glands Of The German Cockroach, Blattella Germanica (L.), Aaron Myers

Open Access Theses

German cockroaches are important urban pests that have been linked to asthma and serious allergic reactions in sensitized individuals. In this research project I, (i) identified different proteins expressed in the tergal glands of male German cockroaches, (ii) determined the expression levels of these proteins in different cockroach life stages and tissues, and (iii) investigated the role of the tergal gland alpha-amylase (BGTG-1) protein. ^ Four major tergal gland proteins were separated on denaturing polyacrylamide gels. With peptide sequencing two of these proteins were identified as alpha-amylase (BGTG-1) and Blattella germanica allergen 2 (Bla g 2). Both of these proteins …

Evolution Of Hematophagous Habit In Triatominae (Heteroptera: Reduviidae), Fernando Otálora-Luna, Antonio J. Pérez-Sánchez, Claudia Sandoval, Elis Aldana

Harold W. Manter Laboratory of Parasitology: Library Materials

All members of Triatominae subfamily (Heteroptera: Reduviidae), potential vectors of Trypanosoma cruzi, etiologic agent of the Chagas disease, feed on blood. Through evolution, these bugs have fixed special morphological, physiological, and behavioral aptations (adaptations and exaptations) adequate to feed on blood. Phylogeny suggests that triatomines evolved from predator reduvids which in turn descended from phytophagous hemipterans. Some pleisiomorphic traits developed by the reduvid ancestors of the triatomines facilitated and modeled hematophagy in these insects. Among them, mouthparts, saliva composition, enzymes, and digestive symbionts are the most noticeable. However, the decisive step that allowed the shift from predation to hematophagy …

The Impacts Of Water Availability On Macroinvertebrate, Ector Matias Martell

Open Access Theses & Dissertations

In many regions around the world climate is changing and biotic communities are changing with it. In areas like the southwestern U.S., drought is one of the major sources of disturbance. Although droughts are an integral part of the natural hydrological cycles, they can have devastating effects on freshwater ecosystems when disrupted by extended periods of drought, resulting in changes to the structure and function of biotic communities. In this study, we surveyed four streams in the Sacramento Mountains of New Mexico from 2010 to 2014: Agua Chiquita, Rio Peñasco, Wills Creek, and the Sacramento River; These years encompassed an …

Sewer Overflows And The Vector Mosquito Proximity To Human West Nile Virus Infections, Andrea Simone Bowers

Walden Dissertations and Doctoral Studies

DeKalb and Fulton Counties, which share the metropolitan Atlanta area, have seen an increase in West Nile infected vector mosquitoes; the increase is associated with close proximity to combined sewer overflow facilities. Despite completion of the remediation system in 2008, the mosquito population testing positive for West Nile virus has increased each year from 2010 through 2012. Guided by the Geographical Information System framework and using spatial analysis and regression analyses, this study described and quantified the relationship between sewer system overflows and amplification of vector mosquitoes; an additional goal was to investigate their proximity to human cases of West …