Entomology Commons^™

Assessing Male Asian Corn Borer, Ostrinia Furnacalis (Guenée) (Lepidoptera: Crambidae), Mating Frequency Via Spermatophore Quantification, Gelyn D. Sapin, Marcela M. Navasero

The Philippine Agricultural Scientist

The Asian corn borer (ACB), Ostrinia furnacalis (Guenée), has caused significant yield losses to corn due to its destructive feeding. Bt corn currently controls ACB, but it still poses a threat to Philippine traditional varieties. Random mating of ACB is essential to the insect resistance management (IRM) of Bt corn, and studying the mating behavior, specifically the mating frequency of ACB, is a critical part of understanding the IRM in the Philippines. Currently, there is no available information on the mating frequency of ACB. Here, the male and female ACB were allowed to mate at a ratio of 1 male …

Oviposition Preference Of The Asian Corn Borer, Ostrinia Furnacalis (Guenée) (Lepidoptera: Crambidae), Towards Bt And Non-Bt Corn, And Under Confined Conditions, Merdelyn T. Caasi-Lit, Jessica B. Baroga-Barbecho, John David C. Cortez, Edwin A. Benigno

The Philippine Agricultural Scientist

Several experiments were conducted on the ovipositional preference and behavior of the Asian corn borer (ACB), Ostrinia furnacalis (Guenée). Confirmatory tests were conducted in table cages (1.5 m²) to determine if the results would align with those obtained from experiments on semi-field (285 m²) and mini-screen cage (90 m²) spatial scales. Preference for Bt and non-Bt corn was then observed under confined conditions. Table cage test results showed similar oviposition behavior to that observed in prior studies on larger test areas. There is no preference for either Bt or non-Bt corn plants. ACB adults …

Adult Dispersal Of Field-Released Asian Corn Borer, Ostrinia Furnacalis (Guenée) (Lepidoptera: Crambidae), In Northern And Southern Philippines, Mario V. Navasero, Randolph N. Candano, Gelyn D. Sapin, Wilson N. De Panis

The Philippine Agricultural Scientist

The effectiveness of Bt corn in controlling the Asian Corn Borer (ACB), Ostrinia furnacalis Guenée, is well recognized. However, concerns persist regarding the potential development of resistance to Bt toxins by this pest. This resistance could arise from surviving ACB individuals carrying homozygous resistance alleles, potentially leading to resistant offspring if mating between resistant individuals is not prevented or minimized. To address this issue, various studies have investigated the factors that limit or facilitate resistance development. These factors include adult dispersal and mating behavior in the field, as well as ecological conditions such as weather and landscape features. However, there …

Review Of Dr. Adelina A. Barrion’S Contributions To Basic Studies On The Asian Corn Borer And A Tribute To An Outstanding Entomologist, Ireneo L. Lit Jr., Merdelyn T. Caasi-Lit, John David C. Cortez, Aimee Lynn A. Barrion-Dupo

The Philippine Agricultural Scientist

The previous works of the late Dr. Adelina A. Barrion, a distinguished Filipino insect geneticist, on the Asian corn borer (ACB), Ostrinia furnacalis (Guenee), were retrieved. The works were reviewed and highlighted in relation to the recently funded project entitled “Basic studies on the Asian corn borer, Ostrinia furnacalis (Guenee),” funded by the Biotechnology Coalition of the Philippines in collaboration with various players in the corn seed industry. The unpublished papers of Dr. Barrion from the 1981 PCARR project entitled “Breeding for Corn Borer Resistance” were important milestones in basic studies on the ACB. Several studies conducted by her team …

Basic Studies On The Asian Corn Borer, Ostrinia Furnacalis (Guenée), Are Crucial For Insect Resistance Management For Bt Corn In The Philippines, Merdelyn T. Caasi-Lit, Aimee Lynn A. Barrion-Dupo

The Philippine Agricultural Scientist

The Philippines is considered to be “at the forefront of biotech research and commercialization in Southeast Asia and a model for science-based and thorough regulatory policy in the region” (ISAAA 2016). This is evident in the country's pioneering adoption of Bt corn technology in Asia and in the effective handling and implementation of rules and regulations on biosafety and the application of transgenic crops through the regulatory function of the Bureau of Plant Industry (BPI) under the Department of Agriculture.

Temporal Decline And Spatial Heterogeneity Of Cry1ab Susceptibility In Asian Corn Borer, Ostrinia Furnacalis (Guenée), Populations In The Philippines: A Review And Synthesis Of Monitoring Data (2015–2024) [Review Article], Edwin P. Alcantara

The Philippine Agricultural Scientist

Transgenic corn expressing the insecticidal Cry1Ab protein has been widely utilized in the Philippines to control the Asian corn borer (ACB), Ostrinia furnacalis (Guenée). Continuous, long-term monitoring is critical to detect shifts in baseline susceptibility and mitigate the risk of field-evolved resistance. This study evaluated the spatiotemporal dynamics of ACB susceptibility to Cry1Ab over nine years (2015–2024). Bioassay data were collected from 12 geographically distinct field populations across the Philippines, alongside a susceptible laboratory reference strain (UPLB). To account for spatial hierarchy and temporal variance, data were analyzed using a Bayesian hierarchical multilevel model with a logit link. Baseline assessments …

Behavioral And Physiological Insights Into Cross-Stage Interactions In The Desert Locust, Benjamin Fürstenau, Vincent O. Nyasembe, Hosea O. Mokaya, Hillary K. Kirwa, Angel Guerrero, Baldwyn Torto

All Peer-Reviewed Publications

Gregarious desert locusts produce stage-specific pheromones that facilitate cohesive behavior in juveniles and synchronize maturation and mating in sexually mature adults. During locust outbreaks, merging populations result in cross-stage interactions, yet their impact on locust biology remains poorly understood. This study tested the hypothesis that cross-stage interactions influence juvenile cohesion and physiological traits. Using behavioral assays and gas chromatography-mass spectrometry, we examined short- and long-term interactions between juvenile and adult desert locusts. In short-term (24 h) cage assays, the presence of adults did not significantly affect grouping behavior in gregarious 3rd instar nymphs, as measured by the mean distance between …

Decomposition Processes And Insect Indicators In Forensic Investigations, Eli Taylor

University Honors Theses

Insects play an essential biological and ecological role in human life, and their predictable relationship with decomposing remains has positioned them as a cornerstone of modern forensic science. Since the early 20th century, forensic entomology has provided critical evidence for estimating the postmortem interval (PMI), relying on patterns of colonization, species succession, and insect developmental timelines. Accurate PMI estimations are vital to medicolegal investigations, influencing the interpretation of biological evidence, guiding resource allocation, and informing determinations of cause and manner of death. However, insect activity is profoundly shaped by environmental and ecological variables, including temperature, humidity, seasonal shifts, and geographic …

Non-Target Organisms And Terrestrial Arthropod Biodiversity In The Corn Agroecosystem: Bt Corn Beyond Acb Pest Management [Review Article], Ireneo L. Lit Jr., Merdelyn T. Caasi-Lit, Kristine O. Abenis

The Philippine Agricultural Scientist

Assessments of risks of modern biotechnology products to non-target organisms (NTOs) are required before a product can be registered and allowed for commercial production. For Bt-engineered plants, data from such studies become part of environmental risk or impact assessments that are reviewed before a hybrid can be registered and allowed for commercial planting. The approaches and methods vary among countries and researchers. Consequently, the choice of species and groups of organisms or guilds that are monitored or studied may range from narrow to broad and encompassing. The inevitability of adverse changes in agroecosystems, including those that involve the corn crop, …

Age-Dependent Reproductive Fitness And Behavioral Dynamics Of The Asian Corn Borer, Ostrinia Furnacalis (Guenée) (Lepidoptera:Crambidae), Marcela M. Navasero, Randolph N. Candano, Maricon Dp Javier, Jethro M. Navasero, Wilson N. De Panis

The Philippine Agricultural Scientist

This study evaluated the effects of parental age of the Asian corn borer (ACB), Ostrinia furnacalis (Guenée), on fecundity, egg hatchability, adult longevity, and the duration of pre-oviposition, oviposition, and post-oviposition periods using arranged mating pairs aged 1 to 5 days old. Mating behavior was also observed and documented. The highest fecundity, measured by the number of egg masses and total eggs produced, was recorded in pairings of 1-day-old males and females, indicating a positive effect of young parental age. In contrast, pairings involving 5-day-old males and females resulted in the lowest egg production, demonstrating an inverse relationship between parental …

Oviposition Preference And Behavior Of The Asian Corn Borer, Ostrinia Furnacalis (Guenée) (Lepidoptera: Crambidae), On Corn And Non-Corn Plants Under Semi-Field And Mini-Screen Cage Conditions, Merdelyn T. Caasi-Lit, Jessica B. Baroga-Barbecho, John David C. Cortez, Edwin A. Benigno

The Philippine Agricultural Scientist

Oviposition site selection in Ostrinia furnacalis is a hierarchical, multi-stage process influenced by plant phenology and spatial context. Oviposition preferences under mini-screen cage (Sampaloc) and semi-field (BL2) conditions were evaluated to determine the effects of field size, corn developmental stage, and the presence of non-corn plants on egg deposition patterns. Females deposited significantly more egg masses on 40 DAP (pre-reproductive) corn than on early vegetative-stage plants [Sampaloc: n=437 (40 DAP), n=88 (30 DAP); BL2: n=98 (40 DAP), n=84 (30 DAP)], demonstrating a strong phenological preference, particularly in Sampaloc. The presence of non-corn plants has a negligible …

Characterization Of Adult Emergence Pattern And Eclosion Behavior Of The Asian Corn Borer, [Ostrinia Furnacalis (Guenée)] (Lepidoptera: Crambidae), Under Laboratory And Field Conditions In The Philippines, Marcela M. Navasero, Gelyn D. Sapin, Merdelyn T. Caasi-Lit, Wilson N. De Panis, Randolph N. Candano, Maricon Dp Javier, Jethro M. Navasero, Meijei T. Bangangao

The Philippine Agricultural Scientist

This study focuses on understanding the emergence pattern of Asian Corn Borer (ACB), Ostrinia furnacalis (Guenee), in the Philippines vital in crafting integrated pest management schemes, including insect resistance management against pesticides and Bt protein. Although ACB adults are known to be nocturnal and primarily emerge at night, identifying critical windows of emergence for targeted management actions is essential. In the experiments conducted, the scotophase period is the preferred time for adult emergence under laboratory conditions with 248 adults (80.52%) and 60 adults (19.48%) in the photophase period. A similar trend was also observed under field cage conditions with ACB …

Movement Of The Different Larval Stages Of Asian Corn Borer, Ostrinia Furnacalis (Guenée), On Conventional Corn Hybrid Under Field Caged Conditions, Merdelyn T. Caasi-Lit, Mark Bryan A. Lontoc, Victor V. Alpuerto, Abigail P. De Leon, Jenifer Bigkas-Naungayan, John David C. Cortez, Edwin A. Benigno

The Philippine Agricultural Scientist

Larval dispersal is crucial in pest population establishment in crop fields. The movement of Ostrinia furnacalis (ACB) larvae within corn plots was observed to determine which factors affect larval dispersal in the field. Conventional corn plots (3 rows, 0.70 m apart x 9 plants, 0.20 m apart) were artificially infested with different combinations of larval age and pest density. The distributions of larvae were recorded through destructive sampling at 4, 8, 12, and 16 days after infestation (DAI). The behavior of ACB from the time the hatching larvae break open the egg mass shell to ballooning to start actual movement …

The Effect Of Corn Event Mir162 And The Triple Stacked Hybrid, Bt11 X Mir162 X Ga21, On Key Non-Target Organisms Of Corn, Ireneo L. Lit Jr., Merdelyn T. Caasi-Lit, Kristine O. Abenis, Edwin A. Benigno

The Philippine Agricultural Scientist

Assessments of environmental risks, especially to non-target organisms, are required before genetically modified plants are approved for commercial production. Incorporated genes or traits, whether as single events or stacked or combined with other traits, are treated separately. In compliance with Philippine regulations, confined field trials were conducted to evaluate possible effects of corn event Maximus MIR162 and triple stacked corn hybrid Bt11 x MIR162 x GA21 on non-target arthropods from August 2011 to January 2012 in Laoac, Pangasinan, and Tampakan, South Cotabato. Visual counts and opportunistic sampling, sweep net sampling, pitfall traps, and sticky traps were used at each sampling …

Arthropod Communities Associated With Endangered Physaria Thamnophila (Zapata Bladderpod) And Its Nurse Plants, Cesar Adrian Hinojosa

Theses and Dissertations

Physaria thamnophila is a federally endangered plant whose associated arthropods are understudied and poorly understood. This study surveyed environmental conditions and the arthropod community within the Arroyo Ramirez Tract of the Lower Rio Grande Valley National Wildlife Refuge, assessed which environmental factors influenced the arthropod community, and sought arthropod taxa statistically associated with Physaria thamnophila. Sticky cards and pitfall traps were deployed for a 7 day period, and vacuum samplings occurred quarterly over the span of a year to collect arthropods that were identified to order and family level for Coleoptera, Hymenoptera, and Lepidoptera. Biotic and abiotic surveys were performed …

Determination Of An Effective Natural Boxelder Bug (Boisea Trivittata) Repellent, Reyna Chavez

Theses and Dissertations

This study investigates the use of ecofriendly repellant alternatives to pest management to control boxelder bugs (Boisea trivittata). This bug gathers by the thousands on warm surfaces of exterior suburban homes during early fall and late spring. I determined bug repellency to turmeric oil (Curcuma longa), catnip (Nepeta cataria), citronella (Cymbopogon nardus), and lavender (Lavandula angustifolia). The treatments consisted of essential oils containing 20% methanol. All treatments demonstrated repellency of Boxelder bugs. Citronella was the most effective and had the highest level of repellency range of 90-99%. Catnip was the least effective with a repellency range of 63-82%.

Reproduction And Larval Development Of The Invasive Asian Longhorned Beetle In South Carolina, Lindsey A. Stone

All Theses

The Asian longhorned beetle (Anoplophora glabripennis Motschulsky; ALB) is a major threat to forest health due to the damage done by larval feeding in host trees. In 2020, ALB was discovered in Charleston County, South Carolina, which presents a novel climate for this invasive pest in North America. I investigated the reproductive biology and larval development of ALB to support detection and management efforts. By exposing 19 host trees to mated pairs of adults, I was able to determine rates of oviposition success (55%), egg viability (55%), and early larval survival (56%). On average, females chewed nearly 1 egg …

Effect Of Planting Date, Insecticide Efficacy, And Hail Injury On Soybean Gall Midge (Resseliella Maxima Gagné) Infestation In Soybean, Natasha Hiromi Umezu

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Resseliella maxima Gagné (Diptera: Cecidomyiidae) is a recently described and economically significant pest of soybean (Glycine max (L.) Merr.) in the Midwestern United States. Since its identification in 2019, this species has become widespread and is now associated with substantial yield losses in parts of the region. Prior to its formal description, orange larvae were observed in soybean associated with plants damaged by hail, suggesting that plant injury may influence infestation. Given the pest’s economic importance, understanding the role of hail injury and developing effective, integrated management strategies are critical for mitigating its impact.

Field studies were conducted to …

Infochemicals For The Management Of Thysanoptera, Nanadagopal Bakthavatsalam, Kesavan Subaharan, Anjenappa Raghavendra, Sevgan Subramaniam

All Peer-Reviewed Publications

Infochemicals, classified as pheromone, kairomone, allomone, synomone and apneumone, play significant role in insect communication. Unlike Lepidoptera, Thysanoptera utilize male produced aggregation pheromone for their communication. Neryl (S)-2-methybutanoate, (R) lavandulyl acetate, (+) R-lavandulyl 3 methyl-butenoate, dodecyl acetate and tetradecyl acetate are the compounds identified as aggregation pheromones for several species. Pheromone lures placed in blue or yellow or white sticky traps are effective in capturing adults of Frankliniella, Thrips, Megalurothips and Odonothrips. Decyl acetate and dodecyl acetate along with other volatile chemicals have been identified as alarm pheromones for many Thysanoptera. Push-Pull technology using alarm pheromone and aggregation pheromone or …

When Thermal Risk Indices Work And When They Don't: A Case Study Of Two Maize Insect Pests, Komi Mensah Agboka, Frank Thomas Ndjomatchoua, Luca Rossini, Ritter A. Guimapi, Elfatih M. Abdel-Rahman

All Peer-Reviewed Publications

The biological life cycle of terrestrial arthropods, using temperature as the primary driving factor has a large interest for insect pests in agriculture, forestry, urban ecosystems, as constitutes the basics for the development of mathematical models for decision making. A recent study proposed a physiologically-based risk index (RI) which finds large applications in the definition of risk maps; however, further case studies are needed to better explore its strengths and limitations. This study aims to extend this knowledge by presenting an application of the RI on two economically significant pests: the fall armyworm Spodoptera frugiperda and the stem borer Busseola …